Name: Pseudonocardia xishanensis DSM 45635
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 13412

Type strain:

Species: Pseudonocardia xishanensis

Culture col. no.: DSM 45635, JCM 17906, KCTC 29005, YIM 63638, BCRC 16943

Strain history: <- S-K Tang, Yunnan Inst., China

NCBI tax ID(s): 630995 (species)

SI-ID 403372

DSM 45635

Section

Pseudonocardia xishanensis DSM 45635 is an aerobe, mesophilic, Gram-positive bacterium that builds an aerial mycelium and was isolated from surface-sterilized roots of Artemisia annua L..

Gram-positive
aerobe
mesophilic
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Pseudonocardiales*
	Family	*Pseudonocardiaceae*
	Genus	*Pseudonocardia*
	Species	**Pseudonocardia xishanensis**
	Full Scientific Name (LPSN)	Pseudonocardia xishanensis Zhao et al. 2012

Information on morphological and physiological properties Morphology

[Ref.: #30479]	Gram stain	positive
[Ref.: #67771]	Gram stain	positive

[Ref.: #30479]	Motility	no

[Ref.: #69413]	Colony color	Beige (1001)
[Ref.: #69413]	Cultivation medium used	ISP 6

[Ref.: #69413]	Colony color	Ivory (1014)
[Ref.: #69413]	Cultivation medium used	ISP 4

[Ref.: #69413]	Colony color	Light ivory (1015)
[Ref.: #69413]	Cultivation medium used	ISP 3

[Ref.: #69413]	Colony color	Sand yellow (1002)
[Ref.: #69413]	Cultivation medium used	suter without tyrosine

[Ref.: #69413]	Colony color	Sand yellow (1002), golden yellow (1004)
[Ref.: #69413]	Cultivation medium used	ISP 2

[Ref.: #30479]	Pigment production	yes
[Ref.: #69413]	Pigment production	yes
[Ref.: #69413]	Pigmentation color	Sand yellow (1002)
[Ref.: #69413]	Pigment name	soluble pigment

[Ref.: #69413]	Multicellular complex forming ability	yes
[Ref.: #69413]	Multicellular complex name	Aerial mycelium
[Ref.: #69413]	Medium Name (multicellularity)	ISP 2

[Ref.: #69413]	Multicellular complex forming ability	yes
[Ref.: #69413]	Multicellular complex name	Aerial mycelium
[Ref.: #69413]	Multicellular complex color	Cream (9001)
[Ref.: #69413]	Medium Name (multicellularity)	ISP 3

[Ref.: #69413]	Multicellular complex forming ability	yes
[Ref.: #69413]	Multicellular complex name	Aerial mycelium
[Ref.: #69413]	Medium Name (multicellularity)	ISP 4

[Ref.: #69413]	Multicellular complex forming ability	yes
[Ref.: #69413]	Multicellular complex name	Aerial mycelium
[Ref.: #69413]	Multicellular complex color	Pure white (6010), brown beige (1011)
[Ref.: #69413]	Medium Name (multicellularity)	ISP 5

[Ref.: #69413]	Multicellular complex forming ability	yes
[Ref.: #69413]	Multicellular complex name	Aerial mycelium
[Ref.: #69413]	Medium Name (multicellularity)	ISP 6

[Ref.: #69413]	Multicellular complex forming ability	yes
[Ref.: #69413]	Multicellular complex name	Aerial mycelium
[Ref.: #69413]	Multicellular complex color	Light ivory (1015), beige (1001)
[Ref.: #69413]	Medium Name (multicellularity)	ISP 7

[Ref.: #69413]	Multicellular complex forming ability	yes
[Ref.: #69413]	Multicellular complex name	Aerial mycelium
[Ref.: #69413]	Multicellular complex color	Beige (1001), cream (9001)
[Ref.: #69413]	Medium Name (multicellularity)	suter with tyrosine

[Ref.: #69413]	Multicellular complex forming ability	yes
[Ref.: #69413]	Multicellular complex name	Aerial mycelium
[Ref.: #69413]	Multicellular complex color	Cream (1002)
[Ref.: #69413]	Medium Name (multicellularity)	suter without tyrosine

Multimedia content

[Ref.: #69413]

Caption:

Plates (65, ISP2, ISP3, ISP4, ISP5, ISP7)

[Ref.: #69413]

Intellectual property rights:

Helmholtz-Zentrum für Infektionsforschung GmbH

[Ref.: #69413]

Caption:

Plates (65, ISP2, ISP3, ISP4, ISP5, ISP7)

[Ref.: #69413]

Intellectual property rights:

Helmholtz-Zentrum für Infektionsforschung GmbH

Information on culture and growth conditions Culture and growth conditions

[Ref.: #17967]

Culture medium

GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)

[Ref.: #17967]

Culture medium growth

[Ref.: #17967]

Culture medium link

Medium recipe at MediaDive

[Ref.: #17967]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #17967]	growth	28 °C
[Ref.: #30479]	growth	10-42 °C
[Ref.: #30479]	optimum	28.5 °C
[Ref.: #67770]	growth	28 °C

[Ref.: #17967]	Temperature range	mesophilic
[Ref.: #30479]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #30479]			optimum	6.5
[Ref.: #30479]			growth	05-09

Information on physiology and metabolism Physiology and metabolism

[Ref.: #30479]	Oxygen tolerance	aerobe
[Ref.: #67771]	Oxygen tolerance	aerobe

	Salt		Salt conc.
[Ref.: #30479]	NaCl	growth	0-7	%
[Ref.: #30479]	NaCl	optimum	1.5	%
[Ref.: #69413]	NaCl	growth	0-5	%

[Ref.: #67770]	Observation	quinones: MK-8(H₄)
[Ref.: #67771]	Observation	quinones: MK-8(H4)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #30479]	arabinose ChEBI	+	carbon source
[Ref.: #69413]	arabinose ChEBI	-	growth
[Ref.: #30479]	cellobiose ChEBI	+	carbon source
[Ref.: #69413]	cellulose ChEBI	-	growth
[Ref.: #68379]	D-glucose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-mannitol ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-ribose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-xylose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	esculin ChEBI	-	hydrolysis	* from API Coryne
[Ref.: #30479]	fructose ChEBI	+	carbon source
[Ref.: #69413]	fructose ChEBI	+/-	growth
[Ref.: #30479]	galactose ChEBI	+	carbon source
[Ref.: #68379]	gelatin ChEBI	-	hydrolysis	* from API Coryne
[Ref.: #30479]	glucose ChEBI	+	carbon source
[Ref.: #69413]	glucose ChEBI	+	growth
[Ref.: #30479]	glycerol ChEBI	+	carbon source
[Ref.: #68379]	glycogen ChEBI	-	fermentation	* from API Coryne
[Ref.: #69413]	inositol ChEBI	+/-	growth
[Ref.: #30479]	lactose ChEBI	+	carbon source
[Ref.: #68379]	lactose ChEBI	-	fermentation	* from API Coryne
[Ref.: #30479]	maltose ChEBI	+	carbon source
[Ref.: #68379]	maltose ChEBI	-	fermentation	* from API Coryne
[Ref.: #30479]	mannitol ChEBI	+	carbon source
[Ref.: #30479]	mannose ChEBI	+	carbon source
[Ref.: #69413]	mannose ChEBI	+/-	growth
[Ref.: #30479]	myo-inositol ChEBI	+	carbon source
[Ref.: #68379]	nitrate ChEBI	-	reduction	* from API Coryne
[Ref.: #69413]	raffinose ChEBI	-	growth
[Ref.: #30479]	rhamnose ChEBI	+	carbon source
[Ref.: #69413]	rhamnose ChEBI	-	growth
[Ref.: #30479]	sorbitol ChEBI	+	carbon source
[Ref.: #30479]	sucrose ChEBI	+	carbon source
[Ref.: #69413]	sucrose ChEBI	+	growth
[Ref.: #68379]	sucrose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	urea ChEBI	-	hydrolysis	* from API Coryne
[Ref.: #30479]	xylose ChEBI	+	carbon source
[Ref.: #69413]	xylose ChEBI	+	growth
	Only first 10 entries are displayed. Click here to see all.

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68379]	alkaline phosphatase	+	3.1.3.1	* from API Coryne, API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68379]	alpha-glucosidase	+	3.2.1.20	* from API Coryne, API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68379]	beta-galactosidase	-	3.2.1.23	* from API Coryne, API zym
[Ref.: #68379]	beta-glucosidase	-	3.2.1.21	* from API Coryne, API zym
[Ref.: #68379]	beta-glucuronidase	-	3.2.1.31	* from API Coryne, API zym
[Ref.: #30479]	catalase	+	1.11.1.6
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #68379]	gelatinase	-		* from API Coryne
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68379]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API Coryne, API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #68379]	pyrazinamidase	-	3.5.1.B15	* from API Coryne
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #30479]	urease	+	3.5.1.5
[Ref.: #68379]	urease	-	3.5.1.5	* from API Coryne
[Ref.: #68382]	valine arylamidase	+		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® Coryne:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitrateNIT	PyrazinamidasePYZ	Pyrrolidonyl arylamidasePYRA	Alkaline phosphatasePAL	beta-Glucuronidasebeta GUR	beta-Galactosidasebeta GAL	alpha-Glucosidasealpha GLU	N-Acetyl-beta-Glucosidasebeta NAG	Esculin hydrolysis/beta-GlucosidaseESC	Urease/urea hydrolysisURE	Gelatinase/gelatin hydrolysisGEL	Control fermentationControl	Fermentation of D-glucoseGLU	Fermentation of D-riboseRIB	Fermentation of D-xyloseXYL	Fermentation of D-mannitolMAN	Fermentation of maltoseMAL	Fermentation of lactoseLAC	Fermentation of sucroseSAC	Fermentation of glycogenGLYG	CatalaseCAT
[Ref.: #69413]	3906	-	-	+/-	+	-	-	+	-	-	-	-	not determinedn.d.	-	-	-	-	-	-	-	-	not determinedn.d.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #69413]	6090	not determinedn.d.	+	+	+	-	+	+	+/-	-	-	+	+	-	-	-	+	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #17967]	Sample type/isolated from	surface-sterilized roots of Artemisia annua L.
[Ref.: #17967]	Host species	Artemisia annua
[Ref.: #17967]	Geographic location (country and/or sea, region)	Yunnan province, Kunming City, Xishan Mountain
[Ref.: #17967]	Country	China
[Ref.: #17967]	Country ISO 3 Code	CHN
[Ref.: #17967]	Continent	Asia

[Ref.: #67770]	Sample type/isolated from	Surface-sterilized roots of Artemisia annua from Xishan Mountain
[Ref.: #67770]	Host species	Artemisia annua
[Ref.: #67770]	Geographic location (country and/or sea, region)	Kunming City, Yunnan Province, south-west China
[Ref.: #67770]	Country	China
[Ref.: #67770]	Country ISO 3 Code	CHN
[Ref.: #67770]	Continent	Asia

[Ref.: #67771]	Sample type/isolated from	From plant
[Ref.: #67771]	Geographic location (country and/or sea, region)	Yunnan Province
[Ref.: #67771]	Country	China
[Ref.: #67771]	Country ISO 3 Code	CHN
[Ref.: #67771]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Host	#Plants	#Herbaceous plants (Grass,Crops)
#Host Body-Site	#Plant	#Root (Rhizome)
#Host Body-Site	#Plant	#Sterilized plant part

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence FJ817397 (>99% sequence identity) for Pseudonocardia xishanensis from Microbeatlas

Aquatic Samples	13
Soil Samples	42
Animal Samples	12
Plant Samples	8
Total Samples	75

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #17967]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #17967]	Pseudonocardia xishanensis strain YIM 63638 16S ribosomal RNA gene, partial sequence	FJ817397	1521	ENA	630995 tax ID

[Ref.: #17967]	GC-content	72.1 mol%	high performance liquid chromatography (HPLC)
[Ref.: #67771]	GC-content	72.1 mol%

Availability in culture collections External links

[Ref.: #17967]

Culture collection no.

DSM 45635, JCM 17906, KCTC 29005, YIM 63638, BCRC 16943

[Ref.: #82609]

SI-ID 403372

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Pseudonocardia pini sp. nov., an endophytic actinobacterium isolated from roots of the pine tree Callitris preissii.	Kaewkla O, Franco CMM	Arch Microbiol	10.1007/s00203-021-02309-3	2021	*
Phylogeny	Pseudonocardia thailandensis sp. nov., an actinomycete isolated from a subterranean termite nest.	Sujarit K, Sujada N, Kudo T, Ohkuma M, Pathom-Aree W, Lumyong S	Int J Syst Evol Microbiol	10.1099/ijsem.0.002017	2017	*
Phylogeny	Pseudonocardia xishanensis sp. nov., an endophytic actinomycete isolated from the roots of Artemisia annua L.	Zhao GZ, Li J, Zhu WY, Wei DQ, Zhang JL, Xu LH, Li WJ	Int J Syst Evol Microbiol	10.1099/ijs.0.037028-0	2011	*

References

#17967

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 45635

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#30479

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #26813 (see below)

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#67771 Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;

#68379

Automatically annotated from API Coryne .

#68382

Automatically annotated from API zym .

#69413

Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#82609

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID403372.1 )

#26813

IJSEM 2395 2012 ( DOI 10.1099/ijs.0.037028-0 , PubMed 22140165 )

* These data were automatically processed and therefore are not curated

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