Azospirillum oryzae (IAM 15130, CCTCC AB 204051, COC8)

Name: Azospirillum oryzae (IAM 15130, CCTCC AB 204051, COC8)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 134118

Type strain

Strain Designation COC8

Culture col. no. IAM 15130 CCTCC AB 204051 COC8 NBRC 102291 BCRC 17901 2 more

NCBI tax ID(s) 286727

Special Collections JCM Literature strains CIP

Links

Azospirillum oryzae COC8 is a facultative aerobe, motile, spiral-shaped prokaryote that was isolated from Rhizosphere of Oryza sativa.

motile spiral-shaped facultative aerobe genome sequence 16S sequence

Name and taxonomic classification

SI-ID 265384

IAM 15130,NBRC 102291,JCM 21588,LMG 23844,CIP 108863

@ref 20215

Last LPSN update 2025-12-16 10:01:58

Domain Pseudomonadati

Phylum Pseudomonadota

Class Alphaproteobacteria

Order Rhodospirillales

Family Azospirillaceae

Genus Azospirillum

Species Azospirillum oryzae

Full scientific name Azospirillum oryzae Xie and Yokota 2005

Morphology

Cell morphology

@ref	Cell length	Cell width	Cell shape	Gram stain	Confidence
31421	3.25 µm	1 µm	spiral-shaped
125439				negative	98.2
125438				negative	98.5
121451			rod-shaped	negative

Culture and growth conditions

Culture medium

@ref	Name	Growth	Composition	Medium link
38346	MEDIUM 72- for trypto casein soja agar		Distilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
121451	CIP Medium 72			Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
31421	positive	growth	04-37
31421	positive	optimum	30	mesophilic
38346	positive	growth	30	mesophilic
67770	positive	growth	27	mesophilic
121451	positive	growth	22-37
121451	negative	growth	10
121451	negative	growth	15
121451	negative	growth	41

Culture pH

@ref	Ability	Type	PH
31421	positive	optimum	6.5

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
31421	facultative aerobe
125439	obligate aerobe	98.9

Spore formation

@ref	Spore formation	Confidence
125439		96.7

Halophily

@ref	Salt	Growth	Tested relation	Concentration
31421	NaCl	positive	growth	<3 %
121451	NaCl	positive	growth	0-10 %

Observation

67770

Observationquinones: Q-10

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68371	27613 ChEBI	amygdalin	-	builds acid from	from API 50CH acid
31421	22599 ChEBI	arabinose	+	carbon source
68371	18305 ChEBI	arbutin	-	builds acid from	from API 50CH acid
68371	17057 ChEBI	cellobiose	-	builds acid from	from API 50CH acid
121451	16947 ChEBI	citrate	+	carbon source
68371	17108 ChEBI	D-arabinose	-	builds acid from	from API 50CH acid
68371	18333 ChEBI	D-arabitol	-	builds acid from	from API 50CH acid
68371	15824 ChEBI	D-fructose	+	builds acid from	from API 50CH acid
68371	12936 ChEBI	D-galactose	-	builds acid from	from API 50CH acid
68371	17634 ChEBI	D-glucose	-	builds acid from	from API 50CH acid
68371	62318 ChEBI	D-lyxose	-	builds acid from	from API 50CH acid
68371	16899 ChEBI	D-mannitol	-	builds acid from	from API 50CH acid
68371	16024 ChEBI	D-mannose	-	builds acid from	from API 50CH acid
68371	16988 ChEBI	D-ribose	-	builds acid from	from API 50CH acid
68371	17924 ChEBI	D-sorbitol	-	builds acid from	from API 50CH acid
68371	16443 ChEBI	D-tagatose	-	builds acid from	from API 50CH acid
68371	65327 ChEBI	D-xylose	-	builds acid from	from API 50CH acid
68371	17113 ChEBI	erythritol	-	builds acid from	from API 50CH acid
31421	4853 ChEBI	esculin	+	hydrolysis
121451	4853 ChEBI	esculin	+	hydrolysis
68371	4853 ChEBI	esculin	+	builds acid from	from API 50CH acid
31421	28757 ChEBI	fructose	+	carbon source
68371	16813 ChEBI	galactitol	-	builds acid from	from API 50CH acid
31421	28260 ChEBI	galactose	+	carbon source
68371	28066 ChEBI	gentiobiose	-	builds acid from	from API 50CH acid
31421	17234 ChEBI	glucose	+	carbon source
121451	17234 ChEBI	glucose	+	fermentation
121451	17234 ChEBI	glucose	+/-	degradation
68371	17754 ChEBI	glycerol	-	builds acid from	from API 50CH acid
68371	28087 ChEBI	glycogen	-	builds acid from	from API 50CH acid
68371	15443 ChEBI	inulin	-	builds acid from	from API 50CH acid
68371	30849 ChEBI	L-arabinose	+	builds acid from	from API 50CH acid
68371	18403 ChEBI	L-arabitol	-	builds acid from	from API 50CH acid
68371	18287 ChEBI	L-fucose	-	builds acid from	from API 50CH acid
68371	62345 ChEBI	L-rhamnose	-	builds acid from	from API 50CH acid
68371	17266 ChEBI	L-sorbose	-	builds acid from	from API 50CH acid
68371	65328 ChEBI	L-xylose	-	builds acid from	from API 50CH acid
121451	17716 ChEBI	lactose	+	fermentation
68371	17716 ChEBI	lactose	-	builds acid from	from API 50CH acid
68371	17306 ChEBI	maltose	-	builds acid from	from API 50CH acid
68371	6731 ChEBI	melezitose	-	builds acid from	from API 50CH acid
68371	28053 ChEBI	melibiose	-	builds acid from	from API 50CH acid
68371	320061 ChEBI	methyl alpha-D-glucopyranoside	-	builds acid from	from API 50CH acid
68371	43943 ChEBI	methyl alpha-D-mannoside	-	builds acid from	from API 50CH acid
68371	74863 ChEBI	methyl beta-D-xylopyranoside	-	builds acid from	from API 50CH acid
68371	17268 ChEBI	myo-inositol	-	builds acid from	from API 50CH acid
68371	59640 ChEBI	N-acetylglucosamine	-	builds acid from	from API 50CH acid
31421	17632 ChEBI	nitrate	+	reduction
121451	17632 ChEBI	nitrate	+	reduction
121451	17632 ChEBI	nitrate	-	respiration
121451	16301 ChEBI	nitrite	-	reduction
68371		Potassium 2-ketogluconate	-	builds acid from	from API 50CH acid
68371		Potassium 5-ketogluconate	-	builds acid from	from API 50CH acid
68371	16634 ChEBI	raffinose	-	builds acid from	from API 50CH acid
31421	26546 ChEBI	rhamnose	+	carbon source
68371	15963 ChEBI	ribitol	-	builds acid from	from API 50CH acid
31421	33942 ChEBI	ribose	+	carbon source
68371	17814 ChEBI	salicin	-	builds acid from	from API 50CH acid
121451	132112 ChEBI	sodium thiosulfate	-	builds gas from
68371	28017 ChEBI	starch	-	builds acid from	from API 50CH acid
68371	17992 ChEBI	sucrose	-	builds acid from	from API 50CH acid
68371	27082 ChEBI	trehalose	-	builds acid from	from API 50CH acid
68371	32528 ChEBI	turanose	-	builds acid from	from API 50CH acid
68371	17151 ChEBI	xylitol	-	builds acid from	from API 50CH acid
31421	18222 ChEBI	xylose	+	carbon source

Metabolite production

@ref	Chebi-ID	Metabolite	Production
121451	35581 ChEBI	indole

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test	Methylred-test
121451	15688 ChEBI	acetoin	-
121451	17234 ChEBI	glucose		-

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
121451	alcohol dehydrogenase	-	1.1.1.1
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	-	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
121451	amylase	-
68382	beta-galactosidase	+	3.2.1.23	from API zym
121451	beta-galactosidase	+	3.2.1.23
68382	beta-glucosidase	+	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
121451	caseinase	-	3.4.21.50
31421	catalase	+	1.11.1.6
121451	catalase	+	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
31421	cytochrome oxidase	+	1.9.3.1
121451	DNase	-
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
121451	gamma-glutamyltransferase	+	2.3.2.2
121451	gelatinase	-
121451	lecithinase	-
68382	leucine arylamidase	+	3.4.11.1	from API zym
121451	lipase	-
68382	lipase (C 14)	-		from API zym
121451	lysine decarboxylase	-	4.1.1.18
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	-		from API zym
121451	ornithine decarboxylase	-	4.1.1.17
121451	oxidase	+
121451	phenylalanine ammonia-lyase	+	4.3.1.24
121451	protease	-
68382	trypsin	-	3.4.21.4	from API zym
121451	tryptophan deaminase	-
121451	tween esterase	+
31421	urease	+	3.5.1.5
121451	urease	+	3.5.1.5
68382	valine arylamidase	-		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
121451	-	+	+	+	-	+	-	-	-	-	+	-	-	+	-	-	+	-	-	-

API 50CHac

@ref	ControlQ	GLY	ERY	DARA	LARA	RIB	DXYL	LXYL	ADO	MDX	GAL	GLU	FRU	MNE	SBE	RHA	DUL	INO	MAN	SOR	MDM	MDG	NAG	AMY	ARB	ESC	SAL	CEL	MAL	LAC	MEL	SAC	TRE	INU	MLZ	RAF	AMD	GLYG	XLT	GEN	TUR	LYX	TAG	DFUC	LFUC	DARL	LARL	GNT	2KG	5KG
121451	not determinedn.d.	-	-	-	+	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+/-	-	-	-	+/-	-	-

Isolation, sampling and environmental information

Isolation

@ref	Sample type	Country	Country ISO 3 Code	Continent	Host species
67770	Rhizosphere of Oryza sativa				Oryza sativa
121451	Plant, Root of rice plant, Oryza saliva	Japan	JPN	Asia

Taxonmaps

69479

Global distribution of 16S sequence AB185396 (>99% sequence identity) for Azospirillum from Microbeatlas

Aquatic Samples	139
Soil Samples	101
Animal Samples	120
Plant Samples	61
Total Samples	421

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
121451	1	Risk group (French classification)

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	NCBI tax ID	Score
66792	ASM836479v1 assembly for Azospirillum oryzae COC8	scaffold	GCA_008364795	286727.5	286727	65.93
124043	ASM3016069v1 assembly for Azospirillum oryzae NBRC 102291	scaffold	GCA_030160695	286727.13	286727	18.41

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
31421	Azospirillum oryzae gene for 16S rRNA, partial sequence	AB185396	1383	286727
124043	Azospirillum oryzae gene for 16S rRNA, partial sequence, strain: NBRC 102291.	AB681747	1387	286727
124043	Azospirillum oryzae strain JCM 21588 16S ribosomal RNA gene, partial sequence.	GU256443	1391	286727

GC content

@ref	GC-content (mol%)	Method
31421	66.8
67770	66.8	high performance liquid chromatography (HPLC)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Azospirillum oryzae COC8
NCBI: GCA_008364795

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	96.70	no
125439	motility	BacteriaNetⓘ	yes	78.90	no
125439	gram_stain	BacteriaNetⓘ	negative	98.20	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	98.90	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Azospirillum oryzae COC8
NCBI: GCA_008364795.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	98.50	no
125438	anaerobic	anaerobicⓘ	no	90.05	yes
125438	aerobic	aerobicⓘ	yes	66.31	yes
125438	spore-forming	spore-formingⓘ	no	89.13	no
125438	thermophilic	thermophileⓘ	no	98.44	yes
125438	flagellated	motile2+ⓘ	yes	81.04	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	Genome Sequences of Novel Azospirillum sp. Strains B21 and Sh1, Isolated from Raised Sphagnum Bogs, and Type Strains Azospirillum lipoferum 59b and Azospirillum oryzae COC8.	Grouzdev DS, Tikhonova EN, Kravchenko IK.	Microbiol Resour Announc	10.1128/mra.01174-19	2019
Phylogeny	Azospirillum fermentarium sp. nov., a nitrogen-fixing species isolated from a fermenter.	Lin SY, Liu YC, Hameed A, Hsu YH, Lai WA, Shen FT, Young CC	Int J Syst Evol Microbiol	10.1099/ijs.0.050872-0	2013
Phylogeny	Azospirillum formosense sp. nov., a diazotroph from agricultural soil.	Lin SY, Shen FT, Young LS, Zhu ZL, Chen WM, Young CC	Int J Syst Evol Microbiol	10.1099/ijs.0.030585-0	2011
Phylogeny	Azospirillum oryzae sp. nov., a nitrogen-fixing bacterium isolated from the roots of the rice plant Oryza sativa.	Xie CH, Yokota A	Int J Syst Evol Microbiol	10.1099/ijs.0.63503-0	2005

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#31421	Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #27728 (see below)
#38346	; Curators of the CIP;
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68371	Automatically annotated from API 50CH acid .
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#121451	Collection of Institut Pasteur ; Curators of the CIP; CIP 108863
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

Rate our new design

Content

About us

Azospirillum oryzae (IAM 15130, CCTCC AB 204051, COC8)

Name and taxonomic classification

Morphology

Cell morphology

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Observation

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

API zym

API 50CHac

Isolation, sampling and environmental information

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_008364795

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

Help

Social Media