Name: Azospirillum oryzae COC8
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 134118

Type strain:

Species: Azospirillum oryzae

Strain Designation: COC8

Culture col. no.: IAM 15130, CCTCC AB 204051, JCM 21588, BCRC 17901, NBRC 102291, CIP 108863

Strain history: CIP <- 2005, IAM

NCBI tax ID(s): 286727 (species)

SI-ID 265384

IAM 15130, NBRC 102291, JCM 21588, LMG 23844, CIP 108863

Section

Azospirillum oryzae COC8 is a facultative aerobe, motile, spiral-shaped bacterium that was isolated from Rhizosphere of Oryza sativa.

motile
spiral-shaped
facultative aerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Alphaproteobacteria*
	Order	*Rhodospirillales*
	Family	*Azospirillaceae*
	Genus	*Azospirillum*
	Species	**Azospirillum oryzae**
	Full Scientific Name (LPSN)	Azospirillum oryzae Xie and Yokota 2005

Information on morphological and physiological properties Morphology

[Ref.: #121451]	Gram stain	negative

[Ref.: #121451]	Cell shape	rod-shaped
[Ref.: #31421]	Cell shape	spiral-shaped

[Ref.: #121451]	Motility	yes
[Ref.: #31421]	Motility	yes

[Ref.: #31421]	Cell length	3.25 µm

[Ref.: #31421]	Cell width	1 µm

Information on culture and growth conditions Culture and growth conditions

[Ref.: #38346]

Culture medium

MEDIUM 72- for trypto casein soja agar

[Ref.: #38346]

Culture medium growth

[Ref.: #38346]

Culture medium composition

expand / minimize

[Ref.: #121451]

Culture medium

CIP Medium 72

[Ref.: #121451]

Culture medium growth

[Ref.: #121451]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #31421]		growth	04-37 °C
[Ref.: #31421]		optimum	30 °C
[Ref.: #38346]		growth	30 °C
[Ref.: #67770]		growth	27 °C
[Ref.: #121451]		growth	22-37 °C
[Ref.: #121451]	no	growth	10 °C
[Ref.: #121451]	no	growth	15 °C
[Ref.: #121451]	no	growth	41 °C

[Ref.: #31421]	Temperature range	mesophilic
[Ref.: #38346]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic
[Ref.: #121451]	Temperature range	psychrophilic
[Ref.: #121451]	Temperature range	thermophilic

	pH	Kind of pH		pH
[Ref.: #31421]			optimum	6.5

Information on physiology and metabolism Physiology and metabolism

[Ref.: #31421]

Oxygen tolerance

facultative aerobe

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #31421]		NaCl		growth	<3	%
[Ref.: #121451]		NaCl		growth	0-10	%

[Ref.: #67770]

Observation

quinones: Q-10

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68371]	amygdalin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #31421]	arabinose ChEBI	+	carbon source
[Ref.: #68371]	arbutin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	cellobiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #121451]	citrate ChEBI	+	carbon source
[Ref.: #68371]	D-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fructose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-galactose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-glucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-lyxose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-ribose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-sorbitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-tagatose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	erythritol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #31421]	esculin ChEBI	+	hydrolysis
[Ref.: #121451]	esculin ChEBI	+	hydrolysis
[Ref.: #68371]	esculin ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #31421]	fructose ChEBI	+	carbon source
[Ref.: #68371]	galactitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #31421]	galactose ChEBI	+	carbon source
[Ref.: #68371]	gentiobiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #31421]	glucose ChEBI	+	carbon source
[Ref.: #121451]	glucose ChEBI	+	fermentation
[Ref.: #121451]	glucose ChEBI	+/-	degradation
[Ref.: #68371]	glycerol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycogen ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	inulin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabinose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-rhamnose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-sorbose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #121451]	lactose ChEBI	+	fermentation
[Ref.: #68371]	lactose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	maltose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	melezitose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	melibiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-glucopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-mannoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl beta-D-xylopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	myo-inositol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	N-acetylglucosamine ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #31421]	nitrate ChEBI	+	reduction
[Ref.: #121451]	nitrate ChEBI	+	reduction
[Ref.: #121451]	nitrate ChEBI	-	respiration
[Ref.: #121451]	nitrite ChEBI	-	reduction
[Ref.: #68371]	Potassium 2-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	Potassium 5-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	raffinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #31421]	rhamnose ChEBI	+	carbon source
[Ref.: #68371]	ribitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #31421]	ribose ChEBI	+	carbon source
[Ref.: #68371]	salicin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #121451]	sodium thiosulfate ChEBI	-	builds gas from
[Ref.: #68371]	starch ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	sucrose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	trehalose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	turanose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	xylitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #31421]	xylose ChEBI	+	carbon source
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #121451]		indole ChEBI	no

	Physiological tests	Metabolite	Voges-Proskauer-test	Methylred-test
[Ref.: #121451]		acetoin ChEBI	-
[Ref.: #121451]		glucose ChEBI		-

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #121451]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #121451]	amylase	-
[Ref.: #121451]	beta-galactosidase	+	3.2.1.23
[Ref.: #68382]	beta-galactosidase	+	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	+	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #121451]	caseinase	-	3.4.21.50
[Ref.: #31421]	catalase	+	1.11.1.6
[Ref.: #121451]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #31421]	cytochrome oxidase	+	1.9.3.1
[Ref.: #121451]	DNase	-
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #121451]	gamma-glutamyltransferase	+	2.3.2.2
[Ref.: #121451]	gelatinase	-
[Ref.: #121451]	lecithinase	-
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #121451]	lipase	-
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #121451]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	-		* from API zym
[Ref.: #121451]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #121451]	oxidase	+
[Ref.: #121451]	phenylalanine ammonia-lyase	+	4.3.1.24
[Ref.: #121451]	protease	-
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #121451]	tryptophan deaminase	-
[Ref.: #121451]	tween esterase	+
[Ref.: #31421]	urease	+	3.5.1.5
[Ref.: #121451]	urease	+	3.5.1.5
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® 50CH acid:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	Acid from glycerolGLY	Acid from erythritolERY	Acid from D-arabinoseDARA	Acid from L-arabinoseLARA	Acid from D-riboseRIB	Acid from D-xyloseDXYL	Acid from L-xyloseLXYL	Acid from D-adonitolADO	Acid from methyl beta-D-xylopyranosideMDX	Acid from D-galactoseGAL	Acid from D-glucoseGLU	Acid from D-fructoseFRU	Acid from D-mannoseMNE	Acid from L-sorboseSBE	Acid from L-rhamnoseRHA	Acid from dulcitolDUL	Acid from inositolINO	Acid from D-mannitolMAN	Acid from D-sorbitolSOR	Acid from methyl alpha-D-mannopyranosideMDM	Acid from methyl alpha-D-glucopyranosideMDG	Acid from N-acetyl-glucosamineNAG	Acid from amygdalinAMY	Acid from arbutinARB	Acid from esculinESC	Acid from salicinSAL	Acid from cellobioseCEL	Acid from maltoseMAL	Acid from lactoseLAC	Acid from melibioseMEL	Acid from sucroseSAC	Acid from trehaloseTRE	Acid from inulinINU	Acid from melezitoseMLZ	Acid from raffinoseRAF	Acid from starchAMD	Acid from glycogenGLYG	Acid from xylitolXLT	Acid from gentiobioseGEN	Acid from turanoseTUR	Acid from D-lyxoseLYX	Acid from D-tagatoseTAG	Acid from D-fucoseDFUC	Acid from L-fucoseLFUC	Acid from D-arabitolDARL	Acid from L-arabitolLARL	Acid from gluconateGNT	Acid from 2-Ketogluconate2KG	Acid from 5-Ketogluconate5KG
[Ref.: #121451]	11407	not determinedn.d.	-	-	-	+	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+/-	-	-	-	+/-	-	-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #121451]	11515	-	+	+	+	-	+	-	-	-	-	+	-	-	+	-	-	+	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #67770]	Sample type/isolated from	Rhizosphere of Oryza sativa
[Ref.: #67770]	Host species	Oryza sativa

[Ref.: #121451]	Sample type/isolated from	Plant, Root of rice plant, Oryza saliva
[Ref.: #121451]	Country	Japan
[Ref.: #121451]	Country ISO 3 Code	JPN
[Ref.: #121451]	Continent	Asia
* marker position based on {}

[Ref.: #69479]

Global distribution of 16S sequence AB185396 (>99% sequence identity) for Azospirillum from Microbeatlas

Aquatic Samples	139
Soil Samples	101
Animal Samples	120
Plant Samples	61
Total Samples	421

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #121451]

Biosafety level

Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #31421]	Azospirillum oryzae gene for 16S rRNA, partial sequence	AB185396	1383	GenBank	286727 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Azospirillum oryzae COC8	GCA_008364795	scaffold	GenBank	286727 tax ID	*

[Ref.: #31421]	GC-content	66.8 mol%
[Ref.: #67770]	GC-content	66.8 mol%	high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Azospirillum oryzae COC8 NCBI: GCA_008364795.1
[Ref.: #69480]	motile	yes	88.659	no
[Ref.: #69480]	flagellated	yes	63.243	no
[Ref.: #69480]	gram-positive	no	98.63	no
[Ref.: #69480]	anaerobic	no	96.321	yes
[Ref.: #69480]	aerobic	yes	74.524	no
[Ref.: #69480]	halophile	no	96.071	no
[Ref.: #69480]	spore-forming	no	89.752	no
[Ref.: #69480]	glucose-util	yes	89.898	yes
[Ref.: #69480]	thermophile	no	98.92	yes
[Ref.: #69480]	glucose-ferment	no	89.541	no

Availability in culture collections External links

[Ref.: #31421]

Culture collection no.

IAM 15130, CCTCC AB 204051, JCM 21588, BCRC 17901, NBRC 102291, CIP 108863

[Ref.: #91720]

SI-ID 265384

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Azospirillum fermentarium sp. nov., a nitrogen-fixing species isolated from a fermenter.	Lin SY, Liu YC, Hameed A, Hsu YH, Lai WA, Shen FT, Young CC	Int J Syst Evol Microbiol	10.1099/ijs.0.050872-0	2013	*
Phylogeny	Azospirillum formosense sp. nov., a diazotroph from agricultural soil.	Lin SY, Shen FT, Young LS, Zhu ZL, Chen WM, Young CC	Int J Syst Evol Microbiol	10.1099/ijs.0.030585-0	2011	*
Phylogeny	Azospirillum oryzae sp. nov., a nitrogen-fixing bacterium isolated from the roots of the rice plant Oryza sativa.	Xie CH, Yokota A	Int J Syst Evol Microbiol	10.1099/ijs.0.63503-0	2005	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#31421

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #27728 (see below)

#38346 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68371

Automatically annotated from API 50CH acid .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#91720

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID265384.1 )

#121451 Collection of Institut Pasteur ; Curators of the CIP; CIP 108863

#27728

IJSEM 1435 2005 ( DOI 10.1099/ijs.0.63503-0 , PubMed 16014463 )

* These data were automatically processed and therefore are not curated

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