Name: Pseudonocardia adelaidensis DSM 45352
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 13405

Type strain:

Species: Pseudonocardia adelaidensis

Culture col. no.: DSM 45352, ACM 5286, JCM 18302, EUM 221, EUM 211 (erroneously)

Strain history: DSM 45352 <-- C. Franco EUM 221 <-- O. Kaewkla.

NCBI tax ID(s): 648754 (species)

SI-ID 396567

DSM 45352

Section

Pseudonocardia adelaidensis DSM 45352 is an aerobe, spore-forming, mesophilic bacterium that builds an aerial mycelium and was isolated from surface-sterilized stem of a grey box tree Eucalyptus microcarpa.

Gram-positive
rod-shaped
spore-forming
aerobe
mesophilic
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Pseudonocardiales*
	Family	*Pseudonocardiaceae*
	Genus	*Pseudonocardia*
	Species	**Pseudonocardia adelaidensis**
	Full Scientific Name (LPSN)	Pseudonocardia adelaidensis Kaewkla and Franco 2010

Information on morphological and physiological properties Morphology

[Ref.: #29708]	Gram stain	positive

[Ref.: #29708]	Cell length	1 µm

[Ref.: #29708]	Cell width	0.5 µm

[Ref.: #29708]	Cell shape	rod-shaped

[Ref.: #29708]	Motility	no

[Ref.: #21394]	Colony color	Sand yellow (1002)
[Ref.: #21394]	Incubation period	10-14 days
[Ref.: #21394]	Cultivation medium used	ISP 2

[Ref.: #21394]	Colony color	Ivory (1014)
[Ref.: #21394]	Incubation period	10-14 days
[Ref.: #21394]	Cultivation medium used	ISP 3

[Ref.: #21394]	Colony color	Ivory (1014)
[Ref.: #21394]	Incubation period	10-14 days
[Ref.: #21394]	Cultivation medium used	ISP 4

[Ref.: #21394]	Colony color	Sand yellow (1002)
[Ref.: #21394]	Incubation period	10-14 days
[Ref.: #21394]	Cultivation medium used	ISP 5

[Ref.: #21394]	Colony color	Ivory (1015)
[Ref.: #21394]	Incubation period	10-14 days
[Ref.: #21394]	Cultivation medium used	ISP 7

[Ref.: #21394]	Colony color	Brown beige (1011)
[Ref.: #21394]	Incubation period	10-14 days
[Ref.: #21394]	Cultivation medium used	Suter with tyrosine

[Ref.: #21394]	Colony color	Sand yellow (1002)
[Ref.: #21394]	Incubation period	10-14 days
[Ref.: #21394]	Cultivation medium used	Suter without tyrosine

[Ref.: #29708]

Pigment production

yes

[Ref.: #21394]	Multicellular complex forming ability	no
[Ref.: #21394]	Medium Name (multicellularity)	ISP 2

[Ref.: #21394]	Multicellular complex forming ability	yes
[Ref.: #21394]	Multicellular complex name	Aerial Mycelium
[Ref.: #21394]	Multicellular complex color	Oyster white (1013)
[Ref.: #21394]	Medium Name (multicellularity)	ISP 3

[Ref.: #21394]	Multicellular complex forming ability	yes
[Ref.: #21394]	Multicellular complex name	Aerial Mycelium
[Ref.: #21394]	Multicellular complex color	Light ivory (1015)
[Ref.: #21394]	Medium Name (multicellularity)	ISP 4

[Ref.: #21394]	Multicellular complex forming ability	no
[Ref.: #21394]	Medium Name (multicellularity)	ISP 5

[Ref.: #21394]	Multicellular complex forming ability	no
[Ref.: #21394]	Medium Name (multicellularity)	ISP 7

[Ref.: #21394]	Multicellular complex forming ability	no
[Ref.: #21394]	Medium Name (multicellularity)	Suter with tyrosine

[Ref.: #21394]	Multicellular complex forming ability	no
[Ref.: #21394]	Medium Name (multicellularity)	Suter without tyrosine

Information on culture and growth conditions Culture and growth conditions

[Ref.: #16345]

Culture medium

GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)

[Ref.: #16345]

Culture medium growth

[Ref.: #16345]

Culture medium link

Medium recipe at MediaDive

[Ref.: #16345]

Culture medium composition

expand / minimize

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Culture medium

GPHF-MEDIUM (DSMZ Medium 553)

[Ref.: #16345]

Culture medium growth

[Ref.: #16345]

Culture medium link

Medium recipe at MediaDive

[Ref.: #16345]

Culture medium composition

expand / minimize

[Ref.: #21394]

Culture medium

ISP 2

[Ref.: #21394]

Culture medium growth

[Ref.: #21394]

Culture medium composition

expand / minimize

[Ref.: #21394]

Culture medium

ISP 3

[Ref.: #21394]

Culture medium growth

[Ref.: #21394]

Culture medium composition

expand / minimize

[Ref.: #21394]

Culture medium

ISP 4

[Ref.: #21394]

Culture medium growth

[Ref.: #21394]

Culture medium composition

expand / minimize

[Ref.: #21394]

Culture medium

ISP 5

[Ref.: #21394]

Culture medium growth

[Ref.: #21394]

Culture medium composition

expand / minimize

[Ref.: #21394]

Culture medium

ISP 7

[Ref.: #21394]

Culture medium growth

[Ref.: #21394]

Culture medium composition

expand / minimize

[Ref.: #21394]

Culture medium

Suter with tyrosine

[Ref.: #21394]

Culture medium growth

[Ref.: #21394]

Culture medium composition

expand / minimize

[Ref.: #21394]

Culture medium

Suter without tyrosine

[Ref.: #21394]

Culture medium growth

[Ref.: #21394]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #16345]	growth	28 °C
[Ref.: #29708]	growth	15-45 °C
[Ref.: #29708]	optimum	15-37 °C
[Ref.: #67770]	growth	28 °C

[Ref.: #16345]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #29708]			optimum	06-10
[Ref.: #29708]			growth	06-10

Information on physiology and metabolism Physiology and metabolism

[Ref.: #29708]

Oxygen tolerance

aerobe

[Ref.: #29708]

Ability of spore formation

yes

	Salt		Salt conc.
[Ref.: #21394]	NaCl	growth	0	%
[Ref.: #29708]	NaCl	growth	10-15	%
[Ref.: #29708]	NaCl	optimum	15	%

[Ref.: #29708]	Observation	aggregates in chains
[Ref.: #67770]	Observation	quinones: MK-8(H₄)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #21394]	arabinose ChEBI	+
[Ref.: #21394]	cellulose ChEBI	+
[Ref.: #68379]	D-glucose ChEBI	+	fermentation	* from API Coryne
[Ref.: #68379]	D-mannitol ChEBI	+	fermentation	* from API Coryne
[Ref.: #68379]	D-ribose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-xylose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	esculin ChEBI	+	hydrolysis	* from API Coryne
[Ref.: #21394]	fructose ChEBI	+
[Ref.: #29708]	galactose ChEBI	+	carbon source
[Ref.: #68379]	gelatin ChEBI	+	hydrolysis	* from API Coryne
[Ref.: #21394]	glucose ChEBI	+
[Ref.: #68379]	glycogen ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	lactose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	maltose ChEBI	-	fermentation	* from API Coryne
[Ref.: #21394]	mannose ChEBI	+
[Ref.: #29708]	mannose ChEBI	+	carbon source
[Ref.: #29708]	melibiose ChEBI	+	carbon source
[Ref.: #21394]	myo-inositol ChEBI	-
[Ref.: #68379]	nitrate ChEBI	+	reduction	* from API Coryne
[Ref.: #21394]	raffinose ChEBI	+
[Ref.: #29708]	raffinose ChEBI	+	carbon source
[Ref.: #21394]	rhamnose ChEBI	-
[Ref.: #29708]	rhamnose ChEBI	+	carbon source
[Ref.: #29708]	sorbitol ChEBI	+	carbon source
[Ref.: #21394]	sucrose ChEBI	+
[Ref.: #29708]	sucrose ChEBI	+	carbon source
[Ref.: #29708]	trehalose ChEBI	+	carbon source
[Ref.: #68379]	urea ChEBI	+	hydrolysis	* from API Coryne
[Ref.: #21394]	xylose ChEBI	+
[Ref.: #29708]	xylose ChEBI	+	carbon source
	Only first 10 entries are displayed. Click here to see all.

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68379]	alkaline phosphatase	-	3.1.3.1	* from API Coryne
[Ref.: #68382]	alpha-chymotrypsin	+	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68379]	alpha-glucosidase	-	3.2.1.20	* from API Coryne
[Ref.: #68382]	alpha-mannosidase	+	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	+	3.2.1.23	* from API zym
[Ref.: #68379]	beta-galactosidase	-	3.2.1.23	* from API Coryne
[Ref.: #68379]	beta-glucosidase	+	3.2.1.21	* from API Coryne, API zym
[Ref.: #68379]	beta-glucuronidase	-	3.2.1.31	* from API Coryne, API zym
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #68379]	gelatinase	+		* from API Coryne
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	+	3.2.1.52	* from API zym
[Ref.: #68379]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API Coryne
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	-		* from API zym
[Ref.: #68379]	pyrrolidonyl arylamidase	-	3.4.19.3	* from API Coryne
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #68379]	urease	+	3.5.1.5	* from API Coryne
	Only first 10 entries are displayed. Click here to see all.

API® Coryne:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitrateNIT	PyrazinamidasePYZ	Pyrrolidonyl arylamidasePYRA	Alkaline phosphatasePAL	beta-Glucuronidasebeta GUR	beta-Galactosidasebeta GAL	alpha-Glucosidasealpha GLU	N-Acetyl-beta-Glucosidasebeta NAG	Esculin hydrolysis/beta-GlucosidaseESC	Urease/urea hydrolysisURE	Gelatinase/gelatin hydrolysisGEL	Control fermentationControl	Fermentation of D-glucoseGLU	Fermentation of D-riboseRIB	Fermentation of D-xyloseXYL	Fermentation of D-mannitolMAN	Fermentation of maltoseMAL	Fermentation of lactoseLAC	Fermentation of sucroseSAC	Fermentation of glycogenGLYG	CatalaseCAT
[Ref.: #21394]	520	+	not determinedn.d.	-	-	-	-	-	-	+	+	+	not determinedn.d.	+	-	-	+	-	-	not determinedn.d.	-	not determinedn.d.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #21394]	1035	not determinedn.d.	+	+	+	-	+	+/-	-	-	+	+	-	-	+	-	+/-	+	+	+	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #16345]	Sample type/isolated from	surface-sterilized stem of a grey box tree Eucalyptus microcarpa
[Ref.: #16345]	Host species	Eucalyptus microcarpa
[Ref.: #16345]	Geographic location (country and/or sea, region)	Adelaide, Flinders University campus
[Ref.: #16345]	Country	Australia
[Ref.: #16345]	Country ISO 3 Code	AUS
[Ref.: #16345]	Continent	Australia and Oceania

[Ref.: #67770]	Sample type/isolated from	Surface-sterilized stem of a grey box tree (Eucalyptus microcarpa) on the Flinders University campus
[Ref.: #67770]	Host species	Eucalyptus microcarpa
[Ref.: #67770]	Geographic location (country and/or sea, region)	Adelaide, South Australia
[Ref.: #67770]	Country	Australia
[Ref.: #67770]	Country ISO 3 Code	AUS
[Ref.: #67770]	Continent	Australia and Oceania
* marker position based on {}

Isolation sources categories

#Host	#Plants	#Tree
#Host Body-Site	#Plant	#Stem (Branch)
#Host Body-Site	#Plant	#Sterilized plant part

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #16345]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #16345]	Pseudonocardia adelaidensis strain EUM 221 16S ribosomal RNA gene, partial sequence	FJ805427	1412	ENA	648754 tax ID

[Ref.: #16345]	GC-content	78.8 mol%	high performance liquid chromatography (HPLC)
[Ref.: #29708]	GC-content	78.8 mol%

Availability in culture collections External links

[Ref.: #16345]

Culture collection no.

DSM 45352, ACM 5286, JCM 18302, EUM 221, EUM 211 (erroneously)

[Ref.: #82603]

SI-ID 396567

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Pseudonocardia kunmingensis sp. nov., an actinobacterium isolated from surface-sterilized roots of Artemisia annua L.	Zhao GZ, Li J, Huang HY, Zhu WY, Park DJ, Kim CJ, Xu LH, Li WJ	Int J Syst Evol Microbiol	10.1099/ijs.0.027607-0	2010	*
Phylogeny	Pseudonocardia sichuanensis sp. nov., a novel endophytic actinomycete isolated from the root of Jatropha curcas L.	Qin S, Xing K, Fei SM, Lin Q, Chen XM, Cao CL, Sun Y, Wang Y, Li WJ, Jiang JH	Antonie Van Leeuwenhoek	10.1007/s10482-010-9504-7	2010	*
Phylogeny	Pseudonocardia adelaidensis sp. nov., an endophytic actinobacterium isolated from the surface-sterilized stem of a grey box tree (Eucalyptus microcarpa).	Kaewkla O, Franco CMM	Int J Syst Evol Microbiol	10.1099/ijs.0.019208-0	2010	*

References

#16345

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 45352

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#21394

Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .

#29708

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #26091 (see below)

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68379

Automatically annotated from API Coryne .

#68382

Automatically annotated from API zym .

#82603

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID396567.1 )

#26091

IJSEM 2818 2010 ( DOI 10.1099/ijs.0.019208-0 , PubMed 20081021 )

* These data were automatically processed and therefore are not curated

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