Pseudonocardia tropica DSM 45199 is an aerobe, spore-forming, mesophilic prokaryote that builds an aerial mycelium and was isolated from surface-sterilized stem of Maytenus austroyunnanensis collected from the tropical rainforest.
spore-forming Gram-positive rod-shaped aerobe mesophilic genome sequence 16S sequence| @ref 20215 |
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| Domain Bacillati |
| Phylum Actinomycetota |
| Class Actinomycetes |
| Order Pseudonocardiales |
| Family Pseudonocardiaceae |
| Genus Pseudonocardia |
| Species Pseudonocardia tropica |
| Full scientific name Pseudonocardia tropica Qin et al. 2010 |
| @ref | Gram stain | Cell shape | Motility | |
|---|---|---|---|---|
| 29750 | positive | rod-shaped |
| @ref | Colony color | Incubation period | Medium used | |
|---|---|---|---|---|
| 21391 | Clay brown (8003) | 10-14 days | ISP 2 | |
| 21391 | Clay brown (8003) | 10-14 days | ISP 3 | |
| 21391 | Beige brown (8024) | 10-14 days | ISP 4 | |
| 21391 | Clay brown (8003) | 10-14 days | ISP 5 | |
| 21391 | Ochre brown (8001) | 10-14 days | ISP 7 | |
| 21391 | Nut brown (8011) | 10-14 days | Suter with tyrosine | |
| 21391 | Fawn brown (8007) | 10-14 days | Suter without tyrosine |
| @ref | Forms multicellular complex | Complex name | Complex color | Medium name | |
|---|---|---|---|---|---|
| 21391 | Aerial Mycelium | Pure white (9010) | ISP 2 | ||
| 21391 | Aerial Mycelium | Cream (9001) | ISP 3 | ||
| 21391 | Aerial Mycelium | Traffic white (9016) | ISP 4 | ||
| 21391 | Aerial Mycelium | Beige (1001) | ISP 5 | ||
| 21391 | ISP 7 | ||||
| 21391 | Aerial Mycelium | Oyster white (1013) | Suter with tyrosine | ||
| 21391 | Aerial Mycelium | Light grey (7035) | Suter without tyrosine |
| 29750 | Productionyes |
| @ref: | 15711 |
| multimedia content: | DSM_45199.jpg |
| multimedia.multimedia content: | https://www.dsmz.de/microorganisms/photos/DSM_45199.jpg |
| caption: | Medium 553 28°C |
| intellectual property rights: | © Leibniz-Institut DSMZ |
| manual_annotation: | 1 |
| @ref | Name | Growth | Medium link | Composition | |
|---|---|---|---|---|---|
| 15711 | GPHF-MEDIUM (DSMZ Medium 553) | Medium recipe at MediaDive | Name: GPHF-MEDIUM (DSMZ Medium 553) Composition: Agar 20.0 g/l Glucose 10.0 g/l Beef extract 5.0 g/l Yeast extract 5.0 g/l Casein peptone 5.0 g/l CaCl2 x 2 H2O 0.74 g/l Distilled water | ||
| 21391 | ISP 2 | Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes | |||
| 21391 | ISP 3 | Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms: | |||
| 21391 | ISP 4 | Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml | |||
| 21391 | ISP 5 | Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes | |||
| 21391 | ISP 7 | Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes | |||
| 21391 | Suter with tyrosine | Name: Synthetically suter medium (5337) with and without tyrosine - see Kutzner,H.J., R. M. Kroppensted and F. Korn-Wendisch. (1986) Composition: Glycerol 15.0 g/l Tyrosine 1.0 (optional, see preparation comment) L-arginine 5.0 g/l L-glutamic acid 5.0 g/l L-methionine 0.3 g/l L-isoleucine 0.3 g/l K2HPO4 0.5 g/l MgSO4 x 7 H2O 0.2 g/l Agar 20.0 g/l Trace element solution (5341) 1.0 ml Composition of Trace element solution (5341): CuSO4 x 5 H2O 10.0 g/l CaCl2 x 2 H2O 10.0 g/l FeSO4 x 7 H2O 10.0 g/l ZnSO4 x 7 H2O 10.0 g/l MnSO4 x 7 H2O 40.0 g/l Preparation: Control medium is prepared without tyrosine Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigment Organisms: All Actinomycetes | |||
| 21391 | Suter without tyrosine | Name: Synthetically suter medium (5337) with and without tyrosine - see Kutzner,H.J., R. M. Kroppensted and F. Korn-Wendisch. (1986) Composition: Glycerol 15.0 g/l Tyrosine 1.0 (optional, see preparation comment) L-arginine 5.0 g/l L-glutamic acid 5.0 g/l L-methionine 0.3 g/l L-isoleucine 0.3 g/l K2HPO4 0.5 g/l MgSO4 x 7 H2O 0.2 g/l Agar 20.0 g/l Trace element solution (5341) 1.0 ml Composition of Trace element solution (5341): CuSO4 x 5 H2O 10.0 g/l CaCl2 x 2 H2O 10.0 g/l FeSO4 x 7 H2O 10.0 g/l ZnSO4 x 7 H2O 10.0 g/l MnSO4 x 7 H2O 40.0 g/l Preparation: Control medium is prepared without tyrosine Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigment Organisms: All Actinomycetes |
| 29750 | Oxygen toleranceaerobe |
| 29750 | Spore formationyes |
| @ref | Chebi-ID | Metabolite | Utilization activity | Kind of utilization tested | |
|---|---|---|---|---|---|
| 21391 | 22599 ChEBI | arabinose | + | ||
| 29750 | 17057 ChEBI | cellobiose | + | carbon source | |
| 21391 | 62968 ChEBI | cellulose | + | ||
| 68379 | 17634 ChEBI | D-glucose | - | fermentation | from API Coryne |
| 68379 | 16899 ChEBI | D-mannitol | - | fermentation | from API Coryne |
| 68379 | 16988 ChEBI | D-ribose | - | fermentation | from API Coryne |
| 68379 | 65327 ChEBI | D-xylose | - | fermentation | from API Coryne |
| 68379 | 4853 ChEBI | esculin | - | hydrolysis | from API Coryne |
| 21391 | 28757 ChEBI | fructose | + | ||
| 29750 | 28757 ChEBI | fructose | + | carbon source | |
| 68379 | 5291 ChEBI | gelatin | + | hydrolysis | from API Coryne |
| 21391 | 17234 ChEBI | glucose | + | ||
| 29750 | 17234 ChEBI | glucose | + | carbon source | |
| 68379 | 28087 ChEBI | glycogen | - | fermentation | from API Coryne |
| 68379 | 17716 ChEBI | lactose | - | fermentation | from API Coryne |
| 29750 | 17306 ChEBI | maltose | + | carbon source | |
| 68379 | 17306 ChEBI | maltose | - | fermentation | from API Coryne |
| 29750 | 29864 ChEBI | mannitol | + | carbon source | |
| 21391 | 37684 ChEBI | mannose | + | ||
| 21391 | 17268 ChEBI | myo-inositol | + | ||
| 29750 | 17632 ChEBI | nitrate | + | reduction | |
| 68379 | 17632 ChEBI | nitrate | - | reduction | from API Coryne |
| 21391 | 16634 ChEBI | raffinose | + | ||
| 21391 | 26546 ChEBI | rhamnose | + | ||
| 21391 | 17992 ChEBI | sucrose | + | ||
| 29750 | 17992 ChEBI | sucrose | + | carbon source | |
| 68379 | 16199 ChEBI | urea | - | hydrolysis | from API Coryne |
| 21391 | 18222 ChEBI | xylose | + | ||
| 29750 | 18222 ChEBI | xylose | + | carbon source |
| @ref | Value | Activity | Ec | |
|---|---|---|---|---|
| 68382 | alkaline phosphatase | - | 3.1.3.1 | from API zym |
| 68379 | alkaline phosphatase | - | 3.1.3.1 | from API Coryne |
| 68382 | alpha-chymotrypsin | - | 3.4.21.1 | from API zym |
| 68382 | alpha-fucosidase | - | 3.2.1.51 | from API zym |
| 68382 | alpha-galactosidase | - | 3.2.1.22 | from API zym |
| 68382 | alpha-glucosidase | - | 3.2.1.20 | from API zym |
| 68379 | alpha-glucosidase | - | 3.2.1.20 | from API Coryne |
| 68382 | alpha-mannosidase | - | 3.2.1.24 | from API zym |
| 68382 | beta-galactosidase | - | 3.2.1.23 | from API zym |
| 68379 | beta-galactosidase | - | 3.2.1.23 | from API Coryne |
| 68379 | beta-glucosidase | - | 3.2.1.21 | from API Coryne |
| 68382 | beta-glucuronidase | - | 3.2.1.31 | from API zym |
| 68379 | beta-glucuronidase | - | 3.2.1.31 | from API Coryne |
| 29750 | catalase | + | 1.11.1.6 | |
| 68382 | cystine arylamidase | - | 3.4.11.3 | from API zym |
| 68379 | gelatinase | + | from API Coryne | |
| 68382 | lipase (C 14) | - | from API zym | |
| 68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 | from API zym |
| 68379 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 | from API Coryne |
| 68382 | naphthol-AS-BI-phosphohydrolase | - | from API zym | |
| 68379 | pyrrolidonyl arylamidase | - | 3.4.19.3 | from API Coryne |
| 68382 | trypsin | - | 3.4.21.4 | from API zym |
| 68379 | urease | - | 3.5.1.5 | from API Coryne |
| @ref | Reduction of nitrateNIT | PYZ | PYRA | PAL | beta GUR | beta GAL | alpha GLU | beta NAG | ESC | URE | GEL | Control fermentationControl | GLU | RIB | XYL | MAN | MAL | LAC | SAC | GLYG | CAT | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 21391 | - | not determinedn.d. | - | - | - | - | - | - | - | - | + | not determinedn.d. | - | - | - | - | - | - | not determinedn.d. | - | not determinedn.d. |
| Cat1 | Cat2 | Cat3 | |
|---|---|---|---|
| #Host | #Plants | #Tree | |
| #Host Body-Site | #Plant | #Stem (Branch) | |
| #Host Body-Site | #Plant | #Sterilized plant part | |
| #Climate | #Hot | #Tropical |
| @ref | Sample type | Host species | Geographic location | Country | Country ISO 3 Code | Continent | |
|---|---|---|---|---|---|---|---|
| 15711 | surface-sterilized stem of Maytenus austroyunnanensis collected from the tropical rainforest | Maytenus austroyunnanensis | Yunnan Province, Xishuangbanna | China | CHN | Asia | |
| 67770 | Surface-sterilized stem of Maytenus austroyunnanensis from the tropical rainforest | Maytenus austroyunnanensis | Xishuangbanna, Yunnan Province, south-west China | China | CHN | Asia |
Global distribution of 16S sequence GQ906587 (>99% sequence identity) for Pseudonocardia from Microbeatlas ![]()
| @ref | Description | Assembly level | INSDC accession | NCBI tax ID | Score | |
|---|---|---|---|---|---|---|
| 124043 | ASM4010531v1 assembly for Pseudonocardia tropica JCM 19149 | contig | 681289 | 49.29 | ||
| 124043 | ASM3954459v1 assembly for Pseudonocardia tropica JCM 19149 | scaffold | 681289 | 43.9 |
| @ref | Description | Accession | Length | Database | NCBI tax ID | |
|---|---|---|---|---|---|---|
| 15711 | Pseudonocardia tropica strain YIM 61452 16S ribosomal RNA gene, partial sequence | GQ906587 | 1461 | 681289 |
| Topic | Title | Authors | Journal | DOI | Year | |
|---|---|---|---|---|---|---|
| Phylogeny | Phylogenetic appraisal of antagonistic, slow growing actinomycetes isolated from hypersaline inland solar salterns at Sambhar salt Lake, India. | Jose PA, Jebakumar SR. | Front Microbiol | 10.3389/fmicb.2013.00190 | 2013 | |
| Phylogeny | Pseudonocardia spirodelae sp. nov., isolated from duckweed and formal proposal to reclassify Pseudonocardia antarctica as a later heterotypic synonym of Pseudonocardia alni and reclassify Pseudonocardia carboxydivorans as Pseudonocardia alni subsp. carboxydivorans. | Butdee W, Saimee Y, Suriyachadkun C, Duangmal K. | Int J Syst Evol Microbiol | 10.1099/ijsem.0.006608 | 2025 | |
| Phylogeny | Pseudonocardia nematodicida sp. nov., isolated from mangrove sediment in Hainan, China. | Liu M, Xing SS, Yuan WD, Wei H, Sun QG, Lin XZ, Huang HQ, Bao SX. | Antonie Van Leeuwenhoek | 10.1007/s10482-015-0512-5 | 2015 | |
| Phylogeny | Pseudonocardia tropica sp. nov., an endophytic actinomycete isolated from the stem of Maytenus austroyunnanensis. | Qin S, Zhu WY, Jiang JH, Klenk HP, Li J, Zhao GZ, Xu LH, Li WJ | Int J Syst Evol Microbiol | 10.1099/ijs.0.020099-0 | 2009 |
| #15711 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 45199 |
| #20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 ) |
| #21391 | Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig . |
| #29750 | Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #26132 (see below) |
| #67770 | Japan Collection of Microorganism (JCM) ; Curators of the JCM; |
| #68379 | Automatically annotated from API Coryne . |
| #68382 | Automatically annotated from API zym . |
| #69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta . |
| #124043 | Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy . |
| #126262 | A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 ) |
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