Name: Pseudonocardia tetrahydrofuranoxydans K1
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 13395

Type strain:

Species: Pseudonocardia tetrahydrofuranoxydans

Strain Designation: K1

Culture col. no.: DSM 44239, CIP 109050, CCUG 52126, JCM 14745

Strain history: CIP <- 2005, P. Kämpfer, Giessen Univ., Giessen, Germany <- J.R. Sudreesen: strain K1

NCBI tax ID(s): 1303679 (strain), 102884 (species)

SI-ID 113658

DSM 44239, CIP 109050, CCUG 52126, JCM 14745

Special Collections

Section

Pseudonocardia tetrahydrofuranoxydans K1 is an aerobe, Gram-positive, rod-shaped bacterium that builds an aerial mycelium and was isolated from water of a waste water treatment plant.

Gram-positive
rod-shaped
aerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Pseudonocardiales*
	Family	*Pseudonocardiaceae*
	Genus	*Pseudonocardia*
	Species	**Pseudonocardia tetrahydrofuranoxydans**
	Full Scientific Name (LPSN)	Pseudonocardia tetrahydrofuranoxydans Kämpfer et al. 2006

Information on morphological and physiological properties Morphology

[Ref.: #31757]	Gram stain	positive
[Ref.: #123490]	Gram stain	positive

[Ref.: #31757]	Cell width	1.3 µm

[Ref.: #31757]	Cell shape	rod-shaped
[Ref.: #123490]	Cell shape	rod-shaped

[Ref.: #31757]	Motility	no
[Ref.: #123490]	Motility	no
[Ref.: #69480]	Motility	no Confidence in %94.102

[Ref.: #19854]	Colony color	Saffron yellow
[Ref.: #19854]	Incubation period	10-14 days
[Ref.: #19854]	Cultivation medium used	ISP 2

[Ref.: #19854]	Colony color	Beige
[Ref.: #19854]	Incubation period	10-14 days
[Ref.: #19854]	Cultivation medium used	ISP 3

[Ref.: #19854]	Colony color	Beige
[Ref.: #19854]	Incubation period	10-14 days
[Ref.: #19854]	Cultivation medium used	ISP 4

[Ref.: #19854]	Colony color	Beige
[Ref.: #19854]	Incubation period	10-14 days
[Ref.: #19854]	Cultivation medium used	ISP 5

[Ref.: #19854]	Colony color	Signal yellow
[Ref.: #19854]	Incubation period	10-14 days
[Ref.: #19854]	Cultivation medium used	ISP 6

[Ref.: #19854]	Colony color	Beige
[Ref.: #19854]	Incubation period	10-14 days
[Ref.: #19854]	Cultivation medium used	ISP 7

[Ref.: #59432]	Incubation period	2 days

[Ref.: #31757]

Pigment production

yes

[Ref.: #19854]	Multicellular complex forming ability	yes
[Ref.: #19854]	Multicellular complex name	Aerial Mycelium
[Ref.: #19854]	Multicellular complex color	Cream
[Ref.: #19854]	Medium Name (multicellularity)	ISP 2

[Ref.: #19854]	Multicellular complex forming ability	yes
[Ref.: #19854]	Multicellular complex name	Aerial Mycelium
[Ref.: #19854]	Multicellular complex color	Cream
[Ref.: #19854]	Medium Name (multicellularity)	ISP 3

[Ref.: #19854]	Multicellular complex forming ability	no
[Ref.: #19854]	Medium Name (multicellularity)	ISP 4

[Ref.: #19854]	Multicellular complex forming ability	yes
[Ref.: #19854]	Multicellular complex name	Aerial Mycelium
[Ref.: #19854]	Multicellular complex color	Cream
[Ref.: #19854]	Medium Name (multicellularity)	ISP 5

[Ref.: #19854]	Multicellular complex forming ability	no
[Ref.: #19854]	Medium Name (multicellularity)	ISP 6

[Ref.: #19854]	Multicellular complex forming ability	yes
[Ref.: #19854]	Multicellular complex name	Aerial Mycelium
[Ref.: #19854]	Medium Name (multicellularity)	ISP 7

Information on culture and growth conditions Culture and growth conditions

[Ref.: #11614]

Culture medium

GPHF-MEDIUM (DSMZ Medium 553)

[Ref.: #11614]

Culture medium growth

[Ref.: #11614]

Culture medium link

Medium recipe at MediaDive

[Ref.: #11614]

Culture medium composition

expand / minimize

[Ref.: #11614]

Culture medium

GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)

[Ref.: #11614]

Culture medium growth

[Ref.: #11614]

Culture medium link

Medium recipe at MediaDive

[Ref.: #11614]

Culture medium composition

expand / minimize

[Ref.: #19854]

Culture medium

ISP 2

[Ref.: #19854]

Culture medium growth

[Ref.: #19854]

Culture medium composition

expand / minimize

[Ref.: #19854]

Culture medium

ISP 3

[Ref.: #19854]

Culture medium growth

[Ref.: #19854]

Culture medium composition

expand / minimize

[Ref.: #19854]

Culture medium

ISP 4

[Ref.: #19854]

Culture medium growth

[Ref.: #19854]

Culture medium composition

expand / minimize

[Ref.: #19854]

Culture medium

ISP 5

[Ref.: #19854]

Culture medium growth

[Ref.: #19854]

Culture medium composition

expand / minimize

[Ref.: #19854]

Culture medium

ISP 6

[Ref.: #19854]

Culture medium growth

[Ref.: #19854]

Culture medium composition

expand / minimize

[Ref.: #19854]

Culture medium

ISP 7

[Ref.: #19854]

Culture medium growth

[Ref.: #19854]

Culture medium composition

expand / minimize

[Ref.: #40109]

Culture medium

MEDIUM 57 - for Streptomyces, Nocardioides, Lentzea albidocapillata and Streptoverticillium reticulum

[Ref.: #40109]

Culture medium growth

[Ref.: #40109]

Culture medium composition

expand / minimize

[Ref.: #123490]

Culture medium

CIP Medium 57

[Ref.: #123490]

Culture medium growth

[Ref.: #123490]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #11614]	growth	28 °C
[Ref.: #19854]	optimum	28 °C
[Ref.: #31757]	growth	11-36 °C
[Ref.: #31757]	optimum	25-30 °C
[Ref.: #40109]	growth	30 °C
[Ref.: #59432]	growth	30 °C
[Ref.: #67770]	growth	28 °C

	pH	Kind of pH		pH
[Ref.: #31757]			optimum	7
[Ref.: #31757]			growth	7

Information on physiology and metabolism Physiology and metabolism

[Ref.: #31757]	Oxygen tolerance	aerobe
[Ref.: #59432]	Oxygen tolerance	aerobe
[Ref.: #123490]	Oxygen tolerance	obligate aerobe

[Ref.: #31757]

Ability of spore formation

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #31757]		NaCl		growth	4	%
[Ref.: #31757]		NaCl		optimum	4	%

[Ref.: #31757]	Observation	aggregates in chains
[Ref.: #67770]	Observation	quinones: MK-8(H₄)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #31757]	3-hydroxybenzoate ChEBI	+	carbon source
[Ref.: #31757]	4-hydroxybenzoate ChEBI	+	carbon source
[Ref.: #31757]	lactate ChEBI	+	carbon source
[Ref.: #31757]	malate ChEBI	+	carbon source
[Ref.: #31757]	mannose ChEBI	+	carbon source
[Ref.: #123490]	nitrate ChEBI	+	reduction
[Ref.: #123490]	nitrite ChEBI	-	reduction
[Ref.: #31757]	ribose ChEBI	+	carbon source
[Ref.: #31757]	serine ChEBI	+	carbon source
[Ref.: #31757]	sucrose ChEBI	+	carbon source
[Ref.: #31757]	trehalose ChEBI	+	carbon source
[Ref.: #31757]	xylose ChEBI	+	carbon source
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #123490]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #123490]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #31757]	catalase	+	1.11.1.6
[Ref.: #123490]	catalase	+	1.11.1.6
[Ref.: #31757]	cytochrome oxidase	+	1.9.3.1
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #123490]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #123490]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #123490]	oxidase	-
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #123490]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	+		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #19854]	666	not determinedn.d.	+	-	+	-	+	+	+	-	-	+	+	-	-	-	+	-	-	-	-
[Ref.: #123490]	11633	-	+	+	+	-	+	+	-	-	-	+	+	-	-	-	+	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #11614]	Sample type/isolated from	water of a waste water treatment plant
[Ref.: #11614]	Geographic location (country and/or sea, region)	Göttingen
[Ref.: #11614]	Country	Germany
[Ref.: #11614]	Country ISO 3 Code	DEU
[Ref.: #11614]	Continent	Europe

[Ref.: #59432]	Sample type/isolated from	Sludge,waist water,enrichment with tetrahydrofuran
[Ref.: #59432]	Geographic location (country and/or sea, region)	Göttingen
[Ref.: #59432]	Country	Germany
[Ref.: #59432]	Country ISO 3 Code	DEU
[Ref.: #59432]	Continent	Europe

[Ref.: #67770]	Sample type/isolated from	Sludge from a wastewater treatment plant
[Ref.: #67770]	Geographic location (country and/or sea, region)	Göttingen
[Ref.: #67770]	Country	Germany
[Ref.: #67770]	Country ISO 3 Code	DEU
[Ref.: #67770]	Continent	Europe

[Ref.: #123490]	Sample type/isolated from	Environment, Wash water
[Ref.: #123490]	Geographic location (country and/or sea, region)	Göttingen
[Ref.: #123490]	Country	Germany
[Ref.: #123490]	Country ISO 3 Code	DEU
[Ref.: #123490]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Engineered	#Waste	#Water treatment plant
#Environmental	#Aquatic	-

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #11614]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #123490]	Biosafety level	2	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #11614]	Pseudonocardia tetrahydrofuranoxydans 16S rRNA gene, type strain K1T	AJ249200	1440	GenBank ENA	102884 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Pseudonocardia tetrahydrofuranoxydans JCM 14745	GCA_001313405	contig	GenBank ENA	1303679 tax ID	*
[Ref.: #66792]	Pseudonocardia tetrahydrofuranoxydans JCM 14745	1303679.3	wgs	PATRIC	1303679 tax ID	*
[Ref.: #66792]	Pseudonocardia tetrahydrofuranoxydans JCM 14745	2734481945	draft	JGI IMG/M	1303679 tax ID	*

[Ref.: #31757]	GC-content	71.3 mol%
[Ref.: #67770]	GC-content	71.3 mol%	high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Pseudonocardia tetrahydrofuranoxydans JCM 14745 PATRIC: 1303679.3
[Ref.: #69481]	spore-forming	spore-formingⓘ	yes	70	no

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Pseudonocardia tetrahydrofuranoxydans JCM 14745 PATRIC: 1303679.3
[Ref.: #69480]	gram-positive	gram-positiveⓘ	yes	85.349	yes
[Ref.: #69480]	anaerobic	anaerobicⓘ	no	85.676	yes
[Ref.: #69480]	spore-forming	spore-formingⓘ	no	81.753	no
[Ref.: #69480]	aerobic	aerobicⓘ	yes	75.32	yes
[Ref.: #69480]	thermophilic	thermophileⓘ	no	90.729	yes
[Ref.: #69480]	flagellated	motile2+ⓘ	no	94.102	yes

Availability in culture collections External links

[Ref.: #11614]

Culture collection no.

DSM 44239, CIP 109050, CCUG 52126, JCM 14745

[Ref.: #82592]

SI-ID 113658

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Pseudonocardia sediminis sp. nov., isolated from marine sediment.	Zhang DF, Jiang Z, Li L, Liu BB, Zhang XM, Tian XP, Zhang S, Li WJ	Int J Syst Evol Microbiol	10.1099/ijs.0.057844-0	2013	*
Phylogeny	Pseudonocardia tetrahydrofuranoxydans sp. nov.	Kampfer P, Kohlweyer U, Thiemer B, Andreesen JR	Int J Syst Evol Microbiol	10.1099/ijs.0.64199-0	2006	*

References

#11614

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 44239

#19854

Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#31757

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #28030 (see below)

#40109 ; Curators of the CIP;
#59432 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 52126

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68382

Automatically annotated from API zym .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#82592

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID113658.1 )

#123490 Collection of Institut Pasteur ; Curators of the CIP; CIP 109050

#28030

IJSEM 1535 2006 ( DOI 10.1099/ijs.0.64199-0 , PubMed 16825626 )

* These data were automatically processed and therefore are not curated

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Pseudonocardia tetrahydrofuranoxydans JCM 14745

Pseudonocardia tetrahydrofuranoxydans JCM 14745

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