Pseudonocardia tetrahydrofuranoxydans (DSM 44239, CIP 109050, CCUG 52126)

Name: Pseudonocardia tetrahydrofuranoxydans (DSM 44239, CIP 109050, CCUG 52126)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 13395

Type strain

Strain Designation K1

Culture col. no. DSM 44239 CIP 109050 CCUG 52126 K1 JCM 14745

NCBI tax ID(s) 1303679 102884

Special Collections DSMZ CCUG JCM CIP Wink compendium Literature strains

Links

Pseudonocardia tetrahydrofuranoxydans K1 is an aerobe, mesophilic, Gram-positive prokaryote that builds an aerial mycelium and was isolated from water of a waste water treatment plant.

Gram-positive rod-shaped aerobe mesophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 113658

DSM 44239,CIP 109050,CCUG 52126,JCM 14745

@ref 20215

Last LPSN update 2025-12-16 09:50:05

Domain Bacillati

Phylum Actinomycetota

Class Actinomycetes

Order Pseudonocardiales

Family Pseudonocardiaceae

Genus Pseudonocardia

Species Pseudonocardia tetrahydrofuranoxydans

Full scientific name Pseudonocardia tetrahydrofuranoxydans Kämpfer et al. 2006

Morphology

Cell morphology

@ref	Gram stain	Cell width	Cell shape	Confidence
31757	positive	1.3 µm	rod-shaped
123490	positive		rod-shaped
125438				94.102
125439	positive			99.8

Colony morphology

@ref	Colony color	Incubation period	Medium used
19854	Saffron yellow	10-14 days	ISP 2
19854	Beige	10-14 days	ISP 3
19854	Beige	10-14 days	ISP 4
19854	Beige	10-14 days	ISP 5
19854	Signal yellow	10-14 days	ISP 6
19854	Beige	10-14 days	ISP 7
59432		2 days
123490

Multicellular morphology

@ref	Complex name	Complex color	Medium name
19854	Aerial Mycelium	Cream	ISP 2
19854	Aerial Mycelium	Cream	ISP 3
19854			ISP 4
19854	Aerial Mycelium	Cream	ISP 5
19854			ISP 6
19854	Aerial Mycelium		ISP 7

Pigmentation

31757

Productionyes

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
11614	GPHF-MEDIUM (DSMZ Medium 553)	Medium recipe at MediaDive	Name: GPHF-MEDIUM (DSMZ Medium 553) Composition: Agar 20.0 g/l Glucose 10.0 g/l Beef extract 5.0 g/l Yeast extract 5.0 g/l Casein peptone 5.0 g/l CaCl2 x 2 H2O 0.74 g/l Distilled water
11614	GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)	Medium recipe at MediaDive	Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
19854	ISP 2		Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19854	ISP 3		Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19854	ISP 4		Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19854	ISP 5		Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
19854	ISP 6		Name: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
19854	ISP 7		Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
40109	MEDIUM 57 - for Streptomyces, Nocardioides, Lentzea albidocapillata and Streptoverticillium reticulum		Distilled water make up to (1000.000 ml);Agar (15.000 g);Glucose (4.000g);Yeast extract (4.000 g);Malt extract (10.000 g);Calcium carbonate (2.000 g)
123490	CIP Medium 57	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
11614	positive	growth	28	mesophilic
19854	positive	optimum	28	mesophilic
31757	positive	growth	11-36
31757	positive	optimum	25-30	mesophilic
40109	positive	growth	30	mesophilic
59432	positive	growth	30	mesophilic
67770	positive	growth	28	mesophilic

Culture pH

@ref	Ability	Type	PH
31757	positive	optimum	7
31757	positive	growth	7

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
31757	aerobe
59432	aerobe
123490	obligate aerobe
125439	obligate aerobe	99.3

Spore formation

31757

Spore formationno

Halophily

@ref	Salt	Growth	Tested relation	Concentration
31757	NaCl	positive	growth	4 %
31757	NaCl	positive	optimum	4 %

Observation

@ref	Observation
31757	aggregates in chains
67770	quinones: MK-8(H₄)

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
31757	16193 ChEBI	3-hydroxybenzoate	+	carbon source
31757	17879 ChEBI	4-hydroxybenzoate	+	carbon source
31757	24996 ChEBI	lactate	+	carbon source
31757	25115 ChEBI	malate	+	carbon source
31757	37684 ChEBI	mannose	+	carbon source
123490	17632 ChEBI	nitrate	+	reduction
123490	16301 ChEBI	nitrite	-	reduction
31757	33942 ChEBI	ribose	+	carbon source
31757	17822 ChEBI	serine	+	carbon source
31757	17992 ChEBI	sucrose	+	carbon source
31757	27082 ChEBI	trehalose	+	carbon source
31757	18222 ChEBI	xylose	+	carbon source

Metabolite production

@ref	Chebi-ID	Metabolite	Production
123490	35581 ChEBI	indole

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
123490	alcohol dehydrogenase	-	1.1.1.1
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68382	beta-galactosidase	-	3.2.1.23	from API zym
68382	beta-glucosidase	-	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
31757	catalase	+	1.11.1.6
123490	catalase	+	1.11.1.6
31757	cytochrome oxidase	+	1.9.3.1
68382	esterase lipase (C 8)	+		from API zym
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
123490	lysine decarboxylase	-	4.1.1.18
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
123490	ornithine decarboxylase	-	4.1.1.17
123490	oxidase	-
68382	trypsin	-	3.4.21.4	from API zym
123490	urease	-	3.5.1.5
68382	valine arylamidase	+		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
19854	not determinedn.d.	+	-	+	-	+	+	+	-	-	+	+	-	-	-	+	-	-	-	-
123490	-	+	+	+	-	+	+	-	-	-	+	+	-	-	-	+	-	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Engineered	#Waste	#Water treatment plant
#Environmental	#Aquatic	-

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
11614	water of a waste water treatment plant	Göttingen	Germany	DEU	Europe
59432	Sludge,waist water,enrichment with tetrahydrofuran	Göttingen	Germany	DEU	Europe
67770	Sludge from a wastewater treatment plant	Göttingen	Germany	DEU	Europe
123490	Environment, Wash water	Göttingen	Germany	DEU	Europe

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
11614	1	Risk group (German classification) According to TRBA 466
123490	2	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID
66792	ASM131340v1 assembly for Pseudonocardia tetrahydrofuranoxydans JCM 14745	contig	GCA_001313405	1303679.3	2734481945	1303679

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
11614	Pseudonocardia tetrahydrofuranoxydans 16S rRNA gene, type strain K1T	AJ249200	1440		102884

GC content

@ref	GC-content (mol%)	Method
31757	71.3
67770	71.3	high performance liquid chromatography (HPLC)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Pseudonocardia tetrahydrofuranoxydans JCM 14745
NCBI: GCA_001313405

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	56.90	no
125439	motility	BacteriaNetⓘ	no	87.10	no
125439	gram_stain	BacteriaNetⓘ	positive	99.80	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	99.30	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Pseudonocardia tetrahydrofuranoxydans JCM 14745
PATRIC: 1303679.3

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	85.35	yes
125438	anaerobic	anaerobicⓘ	no	85.68	yes
125438	spore-forming	spore-formingⓘ	no	81.75	no
125438	aerobic	aerobicⓘ	yes	75.32	yes
125438	thermophilic	thermophileⓘ	no	90.73	yes
125438	flagellated	motile2+ⓘ	no	94.10	yes

Literature

Topic	Title	Authors	Journal	DOI	Year
Metabolism	Bioconversion of AS1387392: bioconversion studies involving Amycolatopsis azurea JCM 3275.	Sasamura S, Ohsumi K, Takase S, Yamada T, Muramatsu H, Fujie A, Mori H, Fujii T, Hino M, Sakamoto K, Hashimoto M.	J Antibiot (Tokyo)	10.1038/ja.2010.108	2010
	Pseudonocardia pini sp. nov., an endophytic actinobacterium isolated from roots of the pine tree Callitris preissii.	Kaewkla O, Franco CMM.	Arch Microbiol	10.1007/s00203-021-02309-3	2021
Phylogeny	Pseudonocardia sediminis sp. nov., isolated from marine sediment.	Zhang DF, Jiang Z, Li L, Liu BB, Zhang XM, Tian XP, Zhang S, Li WJ	Int J Syst Evol Microbiol	10.1099/ijs.0.057844-0	2013
Phylogeny	Pseudonocardia tetrahydrofuranoxydans sp. nov.	Kampfer P, Kohlweyer U, Thiemer B, Andreesen JR	Int J Syst Evol Microbiol	10.1099/ijs.0.64199-0	2006

References

#11614	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 44239
#19854	Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#28030	IJSEM 1535 2006 ( DOI 10.1099/ijs.0.64199-0 , PubMed 16825626 )
#31757	Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #28030
#40109	; Curators of the CIP;
#59432	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 52126
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68382	Automatically annotated from API zym .
#123490	Collection of Institut Pasteur ; Curators of the CIP; CIP 109050
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

Rate our new design

Content

About us

Pseudonocardia tetrahydrofuranoxydans (DSM 44239, CIP 109050, CCUG 52126)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Multicellular morphology

Pigmentation

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Observation

Metabolite utilization

Metabolite production

Enzymes

API zym

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_001313405

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

Help

Social Media