Pseudonocardia dioxanivorans (DSM 44775, ATCC 55486, JCM 13855)

Cell morphology

Colony morphology

Multicellular morphology

Multimedia

• @ref:	12080
  multimedia content:	DSM_44775.jpg
  multimedia.multimedia content:	https://www.dsmz.de/microorganisms/photos/DSM_44775.jpg
  intellectual property rights:	© Leibniz-Institut DSMZ
  manual_annotation:	1

Culture medium

@ref	Name	Growth	Medium link	Composition
12080	GPHF-MEDIUM (DSMZ Medium 553)		Medium recipe at MediaDive	Name: GPHF-MEDIUM (DSMZ Medium 553) Composition: Agar 20.0 g/l Glucose 10.0 g/l Beef extract 5.0 g/l Yeast extract 5.0 g/l Casein peptone 5.0 g/l CaCl2 x 2 H2O 0.74 g/l Distilled water
12080	STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252)		Medium recipe at MediaDive	Name: STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252) Composition: Agar 14.985 g/l Starch 9.99001 g/l (NH4)2SO4 1.998 g/l CaCO3 1.998 g/l K2HPO4 0.999001 g/l MgSO4 x 7 H2O 0.999001 g/l NaCl 0.999001 g/l FeSO4 x 7 H2O 0.000999001 g/l MnCl2 x 4 H2O 0.000999001 g/l ZnSO4 x 7 H2O 0.000999001 g/l Distilled water
20113	ISP 2			Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
20113	ISP 3			Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
20113	ISP 4			Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
20113	ISP 5			Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
20113	ISP 6			Name: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
20113	ISP 7			Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes

Culture temp

Oxygen tolerance

Halophily

Observation

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68369	17128 ChEBI	adipate	-	assimilation	from API 20NE
20113	22599 ChEBI	arabinose	+
68369	29016 ChEBI	arginine	-	hydrolysis	from API 20NE
31226	17057 ChEBI	cellobiose	+	carbon source
20113	62968 ChEBI	cellulose	+
68369	17634 ChEBI	D-glucose	+	assimilation	from API 20NE
68369	17634 ChEBI	D-glucose	-	fermentation	from API 20NE
68369	16899 ChEBI	D-mannitol	-	assimilation	from API 20NE
68369	16024 ChEBI	D-mannose	-	assimilation	from API 20NE
68369	27689 ChEBI	decanoate	-	assimilation	from API 20NE
20113	28757 ChEBI	fructose	-
31226	28757 ChEBI	fructose	+	carbon source
31226	28260 ChEBI	galactose	+	carbon source
68369	5291 ChEBI	gelatin	-	hydrolysis	from API 20NE
31226	24265 ChEBI	gluconate	+	carbon source
68369	24265 ChEBI	gluconate	-	assimilation	from API 20NE
20113	17234 ChEBI	glucose	+
31226	21217 ChEBI	L-alaninamide	+	carbon source
68369	30849 ChEBI	L-arabinose	+	assimilation	from API 20NE
68369	25115 ChEBI	malate	-	assimilation	from API 20NE
20113	29864 ChEBI	mannitol	-
31226	29864 ChEBI	mannitol	+	carbon source
20113	17268 ChEBI	myo-inositol	-
68369	59640 ChEBI	N-acetylglucosamine	-	assimilation	from API 20NE
68369	17632 ChEBI	nitrate	-	reduction	from API 20NE
31226	17148 ChEBI	putrescine	+	carbon source
20113	16634 ChEBI	raffinose	+
20113	26546 ChEBI	rhamnose	+
31226	26546 ChEBI	rhamnose	+	carbon source
31226	33942 ChEBI	ribose	+	carbon source
31226	17814 ChEBI	salicin	+	carbon source
31226	30031 ChEBI	succinate	+	carbon source
20113	17992 ChEBI	sucrose	+
31226	27082 ChEBI	trehalose	+	carbon source
68369	27897 ChEBI	tryptophan	-	energy source	from API 20NE
68369	16199 ChEBI	urea	-	hydrolysis	from API 20NE
31226	17151 ChEBI	xylitol	+	carbon source
20113	18222 ChEBI	xylose	+
31226	18222 ChEBI	xylose	+	carbon source

Metabolite production

@ref	Chebi-ID	Metabolite	Production
68369	35581 ChEBI	indole		from API 20NE

Metabolite tests

@ref	Chebi-ID	Metabolite	Indole test
68369	35581 ChEBI	indole	-	from API 20NE

Enzymes

@ref	Value	Activity	Ec
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68369	arginine dihydrolase	-	3.5.3.6	from API 20NE
68382	beta-galactosidase	-	3.2.1.23	from API zym
68382	beta-glucosidase	-	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
68382	cystine arylamidase	-	3.4.11.3	from API zym
68369	gelatinase	-		from API 20NE
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	-		from API zym
68382	trypsin	-	3.4.21.4	from API zym
68369	urease	-	3.5.1.5	from API 20NE

Pathway annotation

Computationally determined by

@ref	pathway	enzyme coverage	annotated reactions	external links
66794	phenylmercury acetate degradation	100	2 of 2
66794	resorcinol degradation	100	2 of 2
66794	glycolate and glyoxylate degradation	100	6 of 6
66794	formaldehyde oxidation	100	3 of 3
66794	adipate degradation	100	2 of 2
66794	coenzyme A metabolism	100	4 of 4
66794	butanoate fermentation	100	4 of 4
66794	methylglyoxal degradation	100	5 of 5
66794	sulfopterin metabolism	100	4 of 4
66794	ethanol fermentation	100	2 of 2
66794	cardiolipin biosynthesis	100	7 of 7
66794	glycogen metabolism	100	5 of 5
66794	vitamin K metabolism	100	5 of 5
66794	valine metabolism	100	9 of 9
66794	enterobactin biosynthesis	100	3 of 3
66794	kanosamine biosynthesis II	100	2 of 2
66794	starch degradation	100	10 of 10
66794	phenylacetate degradation (aerobic)	100	5 of 5
66794	biotin biosynthesis	100	4 of 4
66794	phenol degradation	100	20 of 20
66794	L-lactaldehyde degradation	100	3 of 3
66794	ethylmalonyl-CoA pathway	100	5 of 5
66794	propanol degradation	100	7 of 7
66794	reductive acetyl coenzyme A pathway	100	7 of 7
66794	cis-vaccenate biosynthesis	100	2 of 2
66794	CDP-diacylglycerol biosynthesis	100	2 of 2
66794	UDP-GlcNAc biosynthesis	100	3 of 3
66794	aspartate and asparagine metabolism	100	9 of 9
66794	photosynthesis	100	14 of 14
66794	threonine metabolism	100	10 of 10
66794	ceramide biosynthesis	100	1 of 1
66794	suberin monomers biosynthesis	100	2 of 2
66794	folate polyglutamylation	100	1 of 1
66794	4-hydroxymandelate degradation	100	9 of 9
66794	aerobactin biosynthesis	100	1 of 1
66794	lactate fermentation	100	4 of 4
66794	IAA biosynthesis	100	3 of 3
66794	ppGpp biosynthesis	100	4 of 4
66794	methane metabolism	100	3 of 3
66794	palmitate biosynthesis	100	22 of 22
66794	anapleurotic synthesis of oxalacetate	100	1 of 1
66794	CO2 fixation in Crenarchaeota	100	9 of 9
66794	acetate fermentation	100	4 of 4
66794	citric acid cycle	92.86	13 of 14
66794	leucine metabolism	92.31	12 of 13
66794	phenylalanine metabolism	92.31	12 of 13
66794	degradation of aromatic, nitrogen containing compounds	91.67	11 of 12
66794	pentose phosphate pathway	90.91	10 of 11
66794	propionate fermentation	90	9 of 10
66794	serine metabolism	88.89	8 of 9
66794	NAD metabolism	88.89	16 of 18
66794	molybdenum cofactor biosynthesis	88.89	8 of 9
66794	chorismate metabolism	88.89	8 of 9
66794	vitamin B12 metabolism	88.24	30 of 34
66794	C4 and CAM-carbon fixation	87.5	7 of 8
66794	gluconeogenesis	87.5	7 of 8
66794	ketogluconate metabolism	87.5	7 of 8
66794	isoleucine metabolism	87.5	7 of 8
66794	flavin biosynthesis	86.67	13 of 15
66794	3-phenylpropionate degradation	86.67	13 of 15
66794	peptidoglycan biosynthesis	86.67	13 of 15
66794	heme metabolism	85.71	12 of 14
66794	glutamate and glutamine metabolism	85.71	24 of 28
66794	ubiquinone biosynthesis	85.71	6 of 7
66794	glutathione metabolism	85.71	12 of 14
66794	urea cycle	84.62	11 of 13
66794	tryptophan metabolism	84.21	32 of 38
66794	purine metabolism	82.98	78 of 94
66794	glycolysis	82.35	14 of 17
66794	3-chlorocatechol degradation	80	4 of 5
66794	arachidonate biosynthesis	80	4 of 5
66794	4-hydroxyphenylacetate degradation	80	8 of 10
66794	creatinine degradation	80	4 of 5
66794	gallate degradation	80	4 of 5
66794	hydrogen production	80	4 of 5
66794	Entner Doudoroff pathway	80	8 of 10
66794	factor 420 biosynthesis	80	4 of 5
66794	alanine metabolism	79.31	23 of 29
66794	tetrahydrofolate metabolism	78.57	11 of 14
66794	cysteine metabolism	77.78	14 of 18
66794	vitamin B1 metabolism	76.92	10 of 13
66794	cyclohexanol degradation	75	3 of 4
66794	toluene degradation	75	3 of 4
66794	dTDPLrhamnose biosynthesis	75	6 of 8
66794	glycogen biosynthesis	75	3 of 4
66794	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	75	6 of 8
66794	oxidative phosphorylation	73.63	67 of 91
66794	pyrimidine metabolism	73.33	33 of 45
66794	methionine metabolism	73.08	19 of 26
66794	proline metabolism	72.73	8 of 11
66794	aclacinomycin biosynthesis	71.43	5 of 7
66794	tyrosine metabolism	71.43	10 of 14
66794	lipid metabolism	70.97	22 of 31
66794	arginine metabolism	70.83	17 of 24
66794	sulfate reduction	69.23	9 of 13
66794	isoprenoid biosynthesis	69.23	18 of 26
66794	non-pathway related	68.42	26 of 38
66794	acetyl CoA biosynthesis	66.67	2 of 3
66794	octane oxidation	66.67	2 of 3
66794	selenocysteine biosynthesis	66.67	4 of 6
66794	acetoin degradation	66.67	2 of 3
66794	d-mannose degradation	66.67	6 of 9
66794	cyanate degradation	66.67	2 of 3
66794	sphingosine metabolism	66.67	4 of 6
66794	degradation of pentoses	64.29	18 of 28
66794	metabolism of disaccharids	63.64	7 of 11
66794	vitamin B6 metabolism	63.64	7 of 11
66794	degradation of sugar alcohols	62.5	10 of 16
66794	phosphatidylethanolamine bioynthesis	61.54	8 of 13
66794	degradation of hexoses	61.11	11 of 18
66794	cellulose degradation	60	3 of 5
66794	glycine betaine biosynthesis	60	3 of 5
66794	lipoate biosynthesis	60	3 of 5
66794	bile acid biosynthesis, neutral pathway	58.82	10 of 17
66794	histidine metabolism	58.62	17 of 29
66794	lysine metabolism	57.14	24 of 42
66794	polyamine pathway	56.52	13 of 23
66794	nitrate assimilation	55.56	5 of 9
66794	daunorubicin biosynthesis	55.56	5 of 9
66794	allantoin degradation	55.56	5 of 9
66794	cholesterol biosynthesis	54.55	6 of 11
66794	coenzyme M biosynthesis	50	5 of 10
66794	androgen and estrogen metabolism	50	8 of 16
66794	mannosylglycerate biosynthesis	50	1 of 2
66794	ribulose monophosphate pathway	50	1 of 2
66794	ascorbate metabolism	50	11 of 22
66794	quinate degradation	50	1 of 2
66794	glycine metabolism	50	5 of 10
66794	alginate biosynthesis	50	2 of 4
66794	aminopropanol phosphate biosynthesis	50	1 of 2
66794	pantothenate biosynthesis	50	3 of 6
66794	CMP-KDO biosynthesis	50	2 of 4
66794	phenylpropanoid biosynthesis	46.15	6 of 13
66794	d-xylose degradation	45.45	5 of 11
66794	arachidonic acid metabolism	44.44	8 of 18
66794	lipid A biosynthesis	44.44	4 of 9
66794	chlorophyll metabolism	44.44	8 of 18
66794	benzoyl-CoA degradation	42.86	3 of 7
66794	carotenoid biosynthesis	40.91	9 of 22
66794	myo-inositol biosynthesis	40	4 of 10
66794	bacilysin biosynthesis	40	2 of 5
66794	carnitine metabolism	37.5	3 of 8
66794	(5R)-carbapenem carboxylate biosynthesis	33.33	1 of 3
66794	sulfoquinovose degradation	33.33	1 of 3
66794	degradation of sugar acids	32	8 of 25
66794	mevalonate metabolism	28.57	2 of 7
66794	dolichyl-diphosphooligosaccharide biosynthesis	27.27	3 of 11
66794	catecholamine biosynthesis	25	1 of 4
66794	methanogenesis from CO2	25	3 of 12

API zym

API 20NE

Isolation source categories

Isolation

Taxonmaps

69479

Global distribution of 16S sequence AY340622 (>99% sequence identity) for Pseudonocardia from Microbeatlas

Aquatic Samples	241
Soil Samples	1233
Animal Samples	242
Plant Samples	132
Total Samples	1848

Risk assessment

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	Pseudonocardia dioxanivorans CB1190	complete		675635.11		675635	99.4
66792	ASM19667v2 assembly for Pseudonocardia dioxanivorans CB1190	chromosome	GCA_000196675	675635.36	651053061	675635	87.55

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
31226	Pseudonocardia dioxanivorans 16S ribosomal RNA gene, partial sequence	AY340622	1422		675635

GC content

Predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
Metabolism	Identification and characterization of two types of amino acid-regulated acetyltransferases in actinobacteria.	Lu YX, Liu XX, Liu WB, Ye BC.	Biosci Rep	10.1042/bsr20170157	2017
Enzymology	In vitro characterization of alternative l-threonate and d-erythronate catabolic pathways.	Guo Y, Shen K, Zhang X, Huang H.	Biochem Biophys Res Commun	10.1016/j.bbrc.2023.149440	2024
Genetics	Mining of Microbial Genomes for the Novel Sources of Nitrilases.	Sharma N, Thakur N, Raj T, Savitri, Bhalla TC.	Biomed Res Int	10.1155/2017/7039245	2017
Metabolism	1,4-Dioxane-degrading consortia can be enriched from uncontaminated soils: prevalence of Mycobacterium and soluble di-iron monooxygenase genes.	He Y, Mathieu J, da Silva MLB, Li M, Alvarez PJJ.	Microb Biotechnol	10.1111/1751-7915.12850	2018
	Complete genome sequence of Saccharothrix espanaensis DSM 44229(T) and comparison to the other completely sequenced Pseudonocardiaceae.	Strobel T, Al-Dilaimi A, Blom J, Gessner A, Kalinowski J, Luzhetska M, Puhler A, Szczepanowski R, Bechthold A, Ruckert C.	BMC Genomics	10.1186/1471-2164-13-465	2012
	Profiling microbial community structures and functions in bioremediation strategies for treating 1,4-dioxane-contaminated groundwater.	Miao Y, Heintz MB, Bell CH, Johnson NW, Polasko AL, Favero D, Mahendra S	J Hazard Mater	10.1016/j.jhazmat.2020.124457	2020
	Bioaugmenting the poplar rhizosphere to enhance treatment of 1,4-dioxane.	Simmer R, Mathieu J, da Silva MLB, Lashmit P, Gopishetty S, Alvarez PJJ, Schnoor JL	Sci Total Environ	10.1016/j.scitotenv.2020.140823	2020
	Enhanced long-term attenuation of 1,4-dioxane in bioaugmented flow-through aquifer columns.	da Silva MLB, He Y, Mathieu J, Alvarez PJJ	Biodegradation	10.1007/s10532-020-09903-0	2020
Metabolism	Monitoring, assessment, and prediction of microbial shifts in coupled catalysis and biodegradation of 1,4-dioxane and co-contaminants.	Miao Y, Johnson NW, Phan T, Heck K, Gedalanga PB, Zheng X, Adamson D, Newell C, Wong MS, Mahendra S	Water Res	10.1016/j.watres.2020.115540	2020
Metabolism	Mechanisms of 1,4-Dioxane Biodegradation and Adsorption by Bio-Zeolite in the Presence of Chlorinated Solvents: Experimental and Molecular Dynamics Simulation Studies.	Liu Y, Johnson NW, Liu C, Chen R, Zhong M, Dong Y, Mahendra S	Environ Sci Technol	10.1021/acs.est.9b04154	2019
	Response and recovery of microbial communities subjected to oxidative and biological treatments of 1,4-dioxane and co-contaminants.	Miao Y, Johnson NW, Gedalanga PB, Adamson D, Newell C, Mahendra S	Water Res	10.1016/j.watres.2018.10.070	2018
Metabolism	Co-contaminant effects on 1,4-dioxane biodegradation in packed soil column flow-through systems.	Zhao L, Lu X, Polasko A, Johnson NW, Miao Y, Yang Z, Mahendra S, Gu B	Environ Pollut	10.1016/j.envpol.2018.09.018	2018
Metabolism	Abiotic and bioaugmented granular activated carbon for the treatment of 1,4-dioxane-contaminated water.	Myers MA, Johnson NW, Marin EZ, Pornwongthong P, Liu Y, Gedalanga PB, Mahendra S	Environ Pollut	10.1016/j.envpol.2018.04.011	2018
Genetics	Draft Genome Sequence of the 1,4-Dioxane-Degrading Bacterium Pseudonocardia dioxanivorans BERK-1.	Ramos-Garcia AA, Shankar V, Saski CA, Hsiang T, Freedman DL	Genome Announc	10.1128/genomeA.00207-18	2018
Metabolism	Aerobic biodegradation kinetics for 1,4-dioxane under metabolic and cometabolic conditions.	Barajas-Rodriguez FJ, Freedman DL	J Hazard Mater	10.1016/j.jhazmat.2018.02.030	2018
Phylogeny	Detection and cell sorting of Pseudonocardia species by fluorescence in situ hybridization and flow cytometry using 16S rRNA-targeted oligonucleotide probes.	Li M, Yang Y, He Y, Mathieu J, Yu C, Li Q, Alvarez PJJ	Appl Microbiol Biotechnol	10.1007/s00253-018-8801-3	2018
Metabolism	Synergistic Treatment of Mixed 1,4-Dioxane and Chlorinated Solvent Contaminations by Coupling Electrochemical Oxidation with Aerobic Biodegradation.	Jasmann JR, Gedalanga PB, Borch T, Mahendra S, Blotevogel J	Environ Sci Technol	10.1021/acs.est.7b03134	2017
Metabolism	Hindrance of 1,4-dioxane biodegradation in microcosms biostimulated with inducing or non-inducing auxiliary substrates.	Li M, Liu Y, He Y, Mathieu J, Hatton J, DiGuiseppi W, Alvarez PJ	Water Res	10.1016/j.watres.2017.01.047	2017
Metabolism	1,4-Dioxane degradation potential of members of the genera Pseudonocardia and Rhodococcus.	Inoue D, Tsunoda T, Sawada K, Yamamoto N, Saito Y, Sei K, Ike M	Biodegradation	10.1007/s10532-016-9772-7	2016
Enzymology	Biodegradation Kinetics of 1,4-Dioxane in Chlorinated Solvent Mixtures.	Zhang S, Gedalanga PB, Mahendra S	Environ Sci Technol	10.1021/acs.est.6b02797	2016
Metabolism	Identification of biomarker genes to predict biodegradation of 1,4-dioxane.	Gedalanga PB, Pornwongthong P, Mora R, Chiang SY, Baldwin B, Ogles D, Mahendra S	Appl Environ Microbiol	10.1128/AEM.04162-13	2014
Metabolism	Transition metals and organic ligands influence biodegradation of 1,4-dioxane.	Pornwongthong P, Mulchandani A, Gedalanga PB, Mahendra S	Appl Biochem Biotechnol	10.1007/s12010-014-0841-2	2014
Metabolism	Oxidation of the cyclic ethers 1,4-dioxane and tetrahydrofuran by a monooxygenase in two Pseudonocardia species.	Sales CM, Grostern A, Parales JV, Parales RE, Alvarez-Cohen L	Appl Environ Microbiol	10.1128/AEM.02418-13	2013
Metabolism	RubisCO-based CO2 fixation and C1 metabolism in the actinobacterium Pseudonocardia dioxanivorans CB1190.	Grostern A, Alvarez-Cohen L	Environ Microbiol	10.1111/1462-2920.12144	2013
Phylogeny	Isolation and characterization of bacterial strains that have high ability to degrade 1,4-dioxane as a sole carbon and energy source.	Sei K, Miyagaki K, Kakinoki T, Fukugasako K, Inoue D, Ike M	Biodegradation	10.1007/s10532-012-9614-1	2012
Metabolism	The impact of chlorinated solvent co-contaminants on the biodegradation kinetics of 1,4-dioxane.	Mahendra S, Grostern A, Alvarez-Cohen L	Chemosphere	10.1016/j.chemosphere.2012.10.104	2012
Metabolism	Glyoxylate metabolism is a key feature of the metabolic degradation of 1,4-dioxane by Pseudonocardia dioxanivorans strain CB1190.	Grostern A, Sales CM, Zhuang WQ, Erbilgin O, Alvarez-Cohen L	Appl Environ Microbiol	10.1128/AEM.00067-12	2012
Genetics	Genome sequence of the 1,4-dioxane-degrading Pseudonocardia dioxanivorans strain CB1190.	Sales CM, Mahendra S, Grostern A, Parales RE, Goodwin LA, Woyke T, Nolan M, Lapidus A, Chertkov O, Ovchinnikova G, Sczyrba A, Alvarez-Cohen L	J Bacteriol	10.1128/JB.00415-11	2011
Metabolism	1,4-Dioxane biodegradation at low temperatures in Arctic groundwater samples.	Li M, Fiorenza S, Chatham JR, Mahendra S, Alvarez PJ	Water Res	10.1016/j.watres.2010.02.007	2010
Metabolism	Aerobic biotransformation of polybrominated diphenyl ethers (PBDEs) by bacterial isolates.	Robrock KR, Coelhan M, Sedlak DL, Alvarez-Cohent L	Environ Sci Technol	10.1021/es900411k	2009
Enzymology	Kinetics of 1,4-dioxane biodegradation by monooxygenase-expressing bacteria.	Mahendra S, Alvarez-Cohen L	Environ Sci Technol	10.1021/es060714v	2006
Phylogeny	Pseudonocardia humida sp. nov., an Actinomycete Isolated from Mangrove Soil Showing Distinct Distribution Pattern of Biosynthetic Gene Clusters.	Zan ZY, Ge XF, Huang RR, Liu WZ	Curr Microbiol	10.1007/s00284-022-02784-x	2022
Phylogeny	Pseudonocardia dioxanivorans sp. nov., a novel actinomycete that grows on 1,4-dioxane.	Mahendra S, Alvarez-Cohen L	Int J Syst Evol Microbiol	10.1099/ijs.0.63085-0	2005