Name: Pseudonocardia spinosispora LM 141
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 13391

Type strain:

Species: Pseudonocardia spinosispora

Strain Designation: LM 141

Culture col. no.: DSM 44797, IMSNU 50581, JCM 11935, KCTC 9991, NRRL B-24156, BCRC 16382, CIP 108013

Strain history: CIP <- 2003, KCTC <- SD Lee, Seoul Natl Univ

NCBI tax ID(s): 1123025 (strain), 103441 (species)

SI-ID 100892

KCTC 9991, NRRL B-24156, CIP 108013, DSM 44797, BCRC 16382, CCRC 16382

Other strains from Pseudonocardia spinosispora

P. spinosispora STI43373(IMET), IMET 43351

Section

Pseudonocardia spinosispora LM 141 is an aerobe, spore-forming, mesophilic bacterium that was isolated from soil from gold mine cave.

Gram-positive
spore-forming
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Pseudonocardiales*
	Family	*Pseudonocardiaceae*
	Genus	*Pseudonocardia*
	Species	**Pseudonocardia spinosispora**
	Full Scientific Name (LPSN)	Pseudonocardia spinosispora Lee et al. 2002

Information on morphological and physiological properties Morphology

[Ref.: #67771]	Gram stain	positive
[Ref.: #123262]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #123262]	Cell shape	rod-shaped

[Ref.: #123262]	Motility	no
[Ref.: #69480]	Motility	no Confidence in %92.156

Information on culture and growth conditions Culture and growth conditions

[Ref.: #11984]

Culture medium

GPHF-MEDIUM (DSMZ Medium 553)

[Ref.: #11984]

Culture medium growth

[Ref.: #11984]

Culture medium link

Medium recipe at MediaDive

[Ref.: #11984]

Culture medium composition

expand / minimize

[Ref.: #33191]

Culture medium

MEDIUM 57 - for Streptomyces, Nocardioides, Lentzea albidocapillata and Streptoverticillium reticulum

[Ref.: #33191]

Culture medium growth

[Ref.: #33191]

Culture medium composition

expand / minimize

[Ref.: #123262]

Culture medium

CIP Medium 57

[Ref.: #123262]

Culture medium growth

[Ref.: #123262]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #11984]		growth	28 °C
[Ref.: #33191]		growth	30 °C
[Ref.: #67770]		growth	28 °C
[Ref.: #67771]		growth	30 °C
[Ref.: #123262]		growth	25-30 °C
[Ref.: #123262]	no	growth	10 °C
[Ref.: #123262]	no	growth	41 °C
[Ref.: #123262]	no	growth	45 °C

[Ref.: #11984]	Temperature range	mesophilic
[Ref.: #33191]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic
[Ref.: #67771]	Temperature range	mesophilic
[Ref.: #123262]	Temperature range	mesophilic
[Ref.: #123262]	Temperature range	psychrophilic
[Ref.: #123262]	Temperature range	thermophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #67771]	Oxygen tolerance	aerobe
[Ref.: #123262]	Oxygen tolerance	obligate aerobe

[Ref.: #69481]	Ability of spore formation	yes Confidence in %100
[Ref.: #69480]	Ability of spore formation	yes Confidence in %98.605

	Salt	Tested relation		Salt conc.
[Ref.: #123262]	NaCl		growth	0-6	%
[Ref.: #123262]	NaCl	no	growth	8	%
[Ref.: #123262]	NaCl	no	growth	10	%

[Ref.: #67770]

Observation

quinones: MK-8(H₄)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #123262]	citrate ChEBI	-	carbon source
[Ref.: #123262]	esculin ChEBI	-	hydrolysis
[Ref.: #123262]	hippurate ChEBI	-	hydrolysis
[Ref.: #123262]	nitrate ChEBI	+	reduction
[Ref.: #123262]	nitrate ChEBI	-	respiration
[Ref.: #123262]	nitrite ChEBI	-	reduction
[Ref.: #123262]	sodium thiosulfate ChEBI	-	builds gas from

	Antibiotica	Metabolite	Antibiotic sensitivity	Antibiotic resistance
[Ref.: #123262]		0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)	no	yes

	Metabolite production	Metabolite	Production
[Ref.: #123262]		indole ChEBI	no

	Physiological tests	Metabolite	Voges-Proskauer-test	Methylred-test
[Ref.: #123262]		acetoin ChEBI	-
[Ref.: #123262]		glucose ChEBI		-

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #123262]	alcohol dehydrogenase	+	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	-	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #123262]	amylase	-
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #123262]	caseinase	-	3.4.21.50
[Ref.: #123262]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #123262]	DNase	-
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	-		* from API zym
[Ref.: #123262]	gamma-glutamyltransferase	-	2.3.2.2
[Ref.: #123262]	gelatinase	-
[Ref.: #123262]	lecithinase	-
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #123262]	lipase	-
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #123262]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #123262]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #123262]	oxidase	+
[Ref.: #123262]	phenylalanine ammonia-lyase	-	4.3.1.24
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #123262]	tryptophan deaminase	-
[Ref.: #123262]	tween esterase	-
[Ref.: #123262]	urease	+	3.5.1.5
[Ref.: #68382]	valine arylamidase	+		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® Biotype100:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
[Ref.: #123262]	2541	not determinedn.d.	-	+	+	-	+	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	+	-	-	-	-	-	+	+	-	-	+	+	-	-	-	-	-	-	-	-	-	-	+	+	-	-	-	-	-	-	-	-	-	-	-	+	+	+	-	-	+	-	-	-	-	-	-	-	-	+	-	-	+	+	+	+	+	-	-	-	-	+	+	+	+	+	+	-	+	+	-	+	+

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #123262]	10821	-	-	+	-	-	+	+	-	-	-	+	+	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #11984]	Sample type/isolated from	soil from gold mine cave
[Ref.: #11984]	Geographic location (country and/or sea, region)	Korea, Kongju City
[Ref.: #11984]	Country	Republic of Korea
[Ref.: #11984]	Country ISO 3 Code	KOR
[Ref.: #11984]	Continent	Asia

[Ref.: #67770]	Sample type/isolated from	Soil at a gold mine cave
[Ref.: #67770]	Geographic location (country and/or sea, region)	near Kongju City
[Ref.: #67770]	Country	Republic of Korea
[Ref.: #67770]	Country ISO 3 Code	KOR
[Ref.: #67770]	Continent	Asia

[Ref.: #67771]	Sample type/isolated from	From soil
[Ref.: #67771]	Country	Republic of Korea
[Ref.: #67771]	Country ISO 3 Code	KOR
[Ref.: #67771]	Continent	Asia

[Ref.: #123262]	Sample type/isolated from	Environment, Soil
[Ref.: #123262]	Geographic location (country and/or sea, region)	Kongju
[Ref.: #123262]	Country	Republic of Korea
[Ref.: #123262]	Country ISO 3 Code	KOR
[Ref.: #123262]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Engineered	#Other	#Mine
#Environmental	#Terrestrial	#Soil

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #11984]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #123262]	Biosafety level	2	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #11984]	Pseudonocardia spinospora partial 16S rRNA gene, isolate LM 141T	AJ249206	1512	ENA	103441 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Pseudonocardia spinosispora DSM 44797	GCA_000429025	scaffold	GenBank	1123025 tax ID
[Ref.: #66792]	Pseudonocardia spinosispora DSM 44797	1123025.3	wgs	PATRIC	1123025 tax ID	*
[Ref.: #66792]	Pseudonocardia spinosispora DSM 44797	2524614769	draft	JGI IMG/M	1123025 tax ID	*

[Ref.: #11984]

GC-content

70.4 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Pseudonocardia spinosispora DSM 44797 DSM 44797 NCBI: GCA_000429025.1
[Ref.: #69481]	spore-forming	yes	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Pseudonocardia spinosispora DSM 44797 PATRIC: 1123025.3
[Ref.: #69480]	motile	no	93.592	no
[Ref.: #69480]	flagellated	no	98.181	no
[Ref.: #69480]	gram-positive	yes	89.053	no
[Ref.: #69480]	anaerobic	no	99.261	yes
[Ref.: #69480]	halophile	no	88.275	no
[Ref.: #69480]	spore-forming	yes	76.945	no
[Ref.: #69480]	glucose-util	yes	88.253	no
[Ref.: #69480]	aerobic	yes	94.577	yes
[Ref.: #69480]	thermophile	no	98.249	no
[Ref.: #69480]	glucose-ferment	no	91.935	no

Availability in culture collections External links

[Ref.: #11984]

Culture collection no.

DSM 44797, IMSNU 50581, JCM 11935, KCTC 9991, NRRL B-24156, BCRC 16382, CIP 108013

[Ref.: #82588]

SI-ID 100892

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Pseudonocardia eucalypti sp. nov., an endophytic actinobacterium with a unique knobby spore surface, isolated from roots of a native Australian eucalyptus tree.	Kaewkla O, Franco CMM	Int J Syst Evol Microbiol	10.1099/ijs.0.022327-0	2010	*
Phylogeny	Pseudonocardia acaciae sp. nov., isolated from roots of Acacia auriculiformis A. Cunn. ex Benth.	Duangmal K, Thamchaipenet A, Matsumoto A, Takahashi Y	Int J Syst Evol Microbiol	10.1099/ijs.0.007724-0	2009	*
Phylogeny	Pseudonocardia spinosispora sp. nov., isolated from Korean soil.	Lee SD, Kim ES, Kang SO, Hah YC	Int J Syst Evol Microbiol	10.1099/00207713-52-5-1603	2002	*

References

#11984

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 44797

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#33191 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#67771 Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;

#68382

Automatically annotated from API zym .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#82588

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID100892.1 )

#123262 Collection of Institut Pasteur ; Curators of the CIP; CIP 108013
* These data were automatically processed and therefore are not curated

Change proposal

You found an error in BacDive? Please tell us about it!

Note that changes will be reviewed and judged. If your changes are legitimate, changes will occur within the next BacDive update. Only proposed changes supported by the according reference will be reviewed. The BacDive team reserves the right to reject proposed changes.

Search for species Pseudonocardia spinosispora in external resources:
SILVA
BRENDA
PANGAEA
StrainInfo
GBIF

Pseudonocardia spinosispora DSM 44797 DSM 44797

Pseudonocardia spinosispora DSM 44797

Change proposal

Change proposal