Name: Pseudonocardia alaniniphila Y-16303
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 13384

Type strain:

Species: Pseudonocardia alaniniphila

Strain Designation: Y-16303

Culture col. no.: DSM 44660, AS 4.1536, CCTCC AA 97001, CIP 107367, JCM 11837, CGMCC 4.1536

Strain history: CIP <- 2002, W.J. Li, Yunnan Univ., Kunming, China: strain Y-16303 <- CCTCC <- C.L. Jiang

NCBI tax ID(s): 75291 (species)

SI-ID 100470

DSM 44660, JCM 11837, AS 4.1536, CIP 107367, CGMCC 4.1536

Section

Pseudonocardia alaniniphila Y-16303 is a facultative anaerobe, mesophilic, Gram-positive bacterium that was isolated from primval forest soil.

Gram-positive
facultative anaerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Pseudonocardiales*
	Family	*Pseudonocardiaceae*
	Genus	*Pseudonocardia*
	Species	**Pseudonocardia alaniniphila**
	Full Scientific Name (LPSN)	Pseudonocardia alaniniphila (Xu et al. 1999) Huang et al. 2002

Synonym

Actinobispora alaniniphila

Information on morphological and physiological properties Morphology

[Ref.: #116174]	Gram stain	positive

[Ref.: #116174]	Motility	no

Multimedia content

[Ref.: #11856]

Caption:

Medium 553 28°C

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Intellectual property rights:

Information on culture and growth conditions Culture and growth conditions

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Culture medium

GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)

[Ref.: #11856]

Culture medium growth

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Culture medium link

Medium recipe at MediaDive

[Ref.: #11856]

Culture medium composition

expand / minimize

[Ref.: #33777]

Culture medium

MEDIUM 116 - for Streptomyces, Nocardia, Streptosporangium and Mycobacterium chlorophenolicum

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Culture medium growth

[Ref.: #33777]

Culture medium composition

expand / minimize

[Ref.: #116174]

Culture medium

CIP Medium 116

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Culture medium growth

[Ref.: #116174]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #11856]		growth	28 °C
[Ref.: #33777]		growth	30 °C
[Ref.: #67770]		growth	28 °C
[Ref.: #116174]		growth	25-41 °C
[Ref.: #116174]	no	growth	10 °C
[Ref.: #116174]	no	growth	45 °C

[Ref.: #11856]	Temperature range	mesophilic
[Ref.: #33777]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic
[Ref.: #116174]	Temperature range	psychrophilic
[Ref.: #116174]	Temperature range	thermophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #116174]

Oxygen tolerance

facultative anaerobe

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #116174]		NaCl		growth	0-10	%

[Ref.: #67770]

Observation

quinones: MK-8(H₄)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #116174]	citrate ChEBI	-	carbon source
[Ref.: #116174]	esculin ChEBI	-	hydrolysis
[Ref.: #116174]	hippurate ChEBI	-	hydrolysis
[Ref.: #116174]	nitrate ChEBI	+	reduction
[Ref.: #116174]	nitrate ChEBI	+	respiration
[Ref.: #116174]	nitrite ChEBI	-	reduction

	Metabolite production	Metabolite	Production
[Ref.: #116174]		indole ChEBI	no

	Physiological tests	Metabolite	Voges-Proskauer-test	Methylred-test
[Ref.: #116174]		acetoin ChEBI	+
[Ref.: #116174]		glucose ChEBI		-

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #116174]	alcohol dehydrogenase	+	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #116174]	beta-galactosidase	-	3.2.1.23
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #116174]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	-		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	-		* from API zym
[Ref.: #116174]	gamma-glutamyltransferase	-	2.3.2.2
[Ref.: #116174]	gelatinase	-
[Ref.: #68382]	leucine arylamidase	-	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #116174]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #116174]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #116174]	oxidase	-
[Ref.: #116174]	phenylalanine ammonia-lyase	-	4.3.1.24
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #116174]	tryptophan deaminase	-
[Ref.: #116174]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #116174]	10301	-	+	-	-	-	-	-	-	-	-	+	+	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #11856]	Sample type/isolated from	primval forest soil
[Ref.: #11856]	Geographic location (country and/or sea, region)	Yunnan, Xichou
[Ref.: #11856]	Country	China
[Ref.: #11856]	Country ISO 3 Code	CHN
[Ref.: #11856]	Continent	Asia

[Ref.: #67770]	Sample type/isolated from	Soil
[Ref.: #67770]	Geographic location (country and/or sea, region)	Xichou, Yunnan
[Ref.: #67770]	Country	China
[Ref.: #67770]	Country ISO 3 Code	CHN
[Ref.: #67770]	Continent	Asia

[Ref.: #116174]	Sample type/isolated from	Environment, Soil
[Ref.: #116174]	Geographic location (country and/or sea, region)	Xichou, Yunnan
[Ref.: #116174]	Country	China
[Ref.: #116174]	Country ISO 3 Code	CHN
[Ref.: #116174]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Environmental	#Terrestrial	#Forest
#Environmental	#Terrestrial	#Soil

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence FR733722 (>99% sequence identity) for Pseudonocardia from Microbeatlas

Aquatic Samples	175
Soil Samples	2822
Animal Samples	266
Plant Samples	735
Total Samples	3998

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #11856]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #116174]	Biosafety level	2	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Pseudonocardia alaniniphila strain YIM 16303 16S ribosomal RNA gene, partial sequence	EU722519	1514	ENA	75291 tax ID
[Ref.: #67770]	Actinobispora alaniniphila 16S ribosomal RNA gene, partial sequence	AF056708	1557	ENA	75291 tax ID
[Ref.: #67770]	Actinobispora alaniniphila 16S ribosomal RNA gene, partial sequence	AF325726	1400	ENA	75291 tax ID
[Ref.: #20218]	Pseudonocardia alaniniphila partial 16S rRNA gene, type strain DSM44660T	FR733722	1512	ENA	75291 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Pseudonocardia alaniniphila Y-16303	GCA_022524485	contig	GenBank	75291 tax ID	*

[Ref.: #67770]

GC-content

69.3 mol%

thermal denaturation, midpoint method (Tm)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Pseudonocardia alaniniphila Y-16303 NCBI: GCA_022524485.1
[Ref.: #69480]	flagellated	no	98.207	no
[Ref.: #69480]	gram-positive	yes	87.49	no
[Ref.: #69480]	anaerobic	no	98.675	no
[Ref.: #69480]	aerobic	yes	90.365	no
[Ref.: #69480]	halophile	no	93.626	no
[Ref.: #69480]	spore-forming	yes	70.719	no
[Ref.: #69480]	motile	no	91.838	no
[Ref.: #69480]	glucose-ferment	no	86.477	no
[Ref.: #69480]	thermophile	no	98.832	yes
[Ref.: #69480]	glucose-util	yes	85.958	no

Availability in culture collections External links

[Ref.: #11856]

Culture collection no.

DSM 44660, AS 4.1536, CCTCC AA 97001, CIP 107367, JCM 11837, CGMCC 4.1536

[Ref.: #82581]

SI-ID 100470

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Pseudonocardia profundimaris sp. nov., isolated from marine sediment.	Zhang G, Wang L, Li J, Zhou Y	Int J Syst Evol Microbiol	10.1099/ijsem.0.001849	2017	*
Phylogeny	Three new species of the genus Actinobispora of the family Pseudonocardiaceae, Actinobispora alaniniphila sp. nov., Actinobispora aurantiaca sp. nov. and Actinobispora xinjiangensis sp. nov.	Xu LH, Jin X, Mao PH, Lu ZF, Cui XL, Jiang CL	Int J Syst Bacteriol	10.1099/00207713-49-2-881	1999	*

References

#11856

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 44660

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#33777 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#82581

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID100470.1 )

#116174 Collection of Institut Pasteur ; Curators of the CIP; CIP 107367
* These data were automatically processed and therefore are not curated

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