Pseudonocardia antitumoralis (DSM 45322, CCTCC M2011255, SCSIO 01299)

Name: Pseudonocardia antitumoralis (DSM 45322, CCTCC M2011255, SCSIO 01299)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 13382

Type strain

Culture col. no. DSM 45322 CCTCC M2011255 SCSIO 01299

NCBI tax ID(s) 1070982

Special Collections DSMZ Wink compendium Literature strains

Links

Pseudonocardia antitumoralis DSM 45322 is an aerobe, spore-forming, mesophilic prokaryote that builds an aerial mycelium and was isolated from deep-sea sediment.

spore-forming Gram-positive rod-shaped aerobe mesophilic 16S sequence

Name and taxonomic classification

SI-ID 403451

DSM 45322

@ref 20215

Last LPSN update 2025-12-16 09:50:04

Domain Bacillati

Phylum Actinomycetota

Class Actinomycetes

Order Pseudonocardiales

Family Pseudonocardiaceae

Genus Pseudonocardia

Species Pseudonocardia antitumoralis

Full scientific name Pseudonocardia antitumoralis Tian et al. 2013

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape
30485	positive	1.9 µm	0.45 µm	rod-shaped

Colony morphology

@ref	Colony color	Medium used
69381	Fawn brown (8007)	ISP 4
69381	Brown beige (1011)	ISP 5
69381	Brown beige (1011)	ISP 6
69381	Brown beige (1011)	suter with tyrosine
69381	Ivory (1014), brown beige (1011)	suter without tyrosine
69381	Ochre brown (8001)	ISP 2
69381	Ochre brown (8001)	ISP 3
69381	Ochre brown (8001)	ISP 7

Multicellular morphology

@ref	Complex name	Complex color	Medium name
69381	Aerial mycelium	Silk grey (7044)	ISP 2
69381	Aerial mycelium	Signal white (9003)	ISP 3
69381	Aerial mycelium		ISP 4
69381	Aerial mycelium	Pure white (9010)	ISP 5
69381	Aerial mycelium	Light ivory (1015)	ISP 6
69381	Aerial mycelium	Light ivory (1015)	ISP 7
69381	Aerial mycelium		suter with tyrosine
69381	Aerial mycelium		suter without tyrosine

Pigmentation

@ref	Production
30485
69381
69381

Multimedia

@ref:	16222
multimedia content:	DSM_45322.jpg
multimedia.multimedia content:	https://www.dsmz.de/microorganisms/photos/DSM_45322.jpg
caption:	Medium 65 28°C
intellectual property rights:	© Leibniz-Institut DSMZ
manual_annotation:	1

@ref:	69381
multimedia content:	DSM_45322_image3.jpeg
multimedia.multimedia content:	DSM_45322_image3.jpeg
caption:	Plates (65, ISP2, ISP3, ISP4, ISP5, ISP7)
intellectual property rights:	Helmholtz-Zentrum für Infektionsforschung GmbH
manual_annotation:	1

@ref:	69381
multimedia content:	DSM_45322_image4.jpeg
multimedia.multimedia content:	DSM_45322_image4.jpeg
caption:	Plates (65, ISP2, ISP3, ISP4, ISP5, ISP7)
intellectual property rights:	Helmholtz-Zentrum für Infektionsforschung GmbH
manual_annotation:	1

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
16222	GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)		Medium recipe at MediaDive	Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
16222	TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)		Medium recipe at MediaDive	Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
16222	positive	growth	28	mesophilic
30485	positive	growth	04-40
30485	positive	optimum	28	mesophilic

Culture pH

@ref	Ability	Type	PH
30485	positive	optimum	7.5
30485	positive	growth	6-8.5

Physiology and metabolism

Oxygen tolerance

30485

Oxygen toleranceaerobe

Spore formation

30485

Spore formationyes

Compound production

16222

Compounddeoxynyboquinone

Halophily

@ref	Salt	Growth	Tested relation	Concentration
30485	NaCl	positive	growth	0-15 %
30485	NaCl	positive	optimum	3 %
69381	NaCl	positive	growth	0-5 %

Observation

30485

Observationaggregates in chains

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
69381	22599 ChEBI	arabinose	-	growth
69381	62968 ChEBI	cellulose	+/-	growth
30485	16947 ChEBI	citrate	+	carbon source
68379	17634 ChEBI	D-glucose	-	fermentation	from API Coryne
68379	16899 ChEBI	D-mannitol	-	fermentation	from API Coryne
68379	16988 ChEBI	D-ribose	-	fermentation	from API Coryne
68379	65327 ChEBI	D-xylose	-	fermentation	from API Coryne
68379	4853 ChEBI	esculin	-	hydrolysis	from API Coryne
69381	28757 ChEBI	fructose	+	growth
69381	17234 ChEBI	glucose	+	growth
68379	28087 ChEBI	glycogen	-	fermentation	from API Coryne
30485	17596 ChEBI	inosine	+	carbon source
69381	17268 ChEBI	inositol	+	growth
30485	17716 ChEBI	lactose	+	carbon source
68379	17716 ChEBI	lactose	-	fermentation	from API Coryne
68379	17306 ChEBI	maltose	-	fermentation	from API Coryne
30485	29864 ChEBI	mannitol	+	carbon source
30485	37684 ChEBI	mannose	+	carbon source
69381	37684 ChEBI	mannose	+	growth
68379	17632 ChEBI	nitrate	-	reduction	from API Coryne
30485	16634 ChEBI	raffinose	+	carbon source
69381	16634 ChEBI	raffinose	+/-	growth
30485	26546 ChEBI	rhamnose	+	carbon source
69381	26546 ChEBI	rhamnose	+	growth
30485	33942 ChEBI	ribose	+	carbon source
30485	30911 ChEBI	sorbitol	+	carbon source
30485	17992 ChEBI	sucrose	+	carbon source
68379	17992 ChEBI	sucrose	-	fermentation	from API Coryne
69381	17992 ChEBI	sucrose	+	growth
68379	16199 ChEBI	urea	-	hydrolysis	from API Coryne
30485	17151 ChEBI	xylitol	+	carbon source
69381	18222 ChEBI	xylose	-	growth

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68379	alkaline phosphatase	+	3.1.3.1	from API Coryne
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68379	alpha-glucosidase	+	3.2.1.20	from API Coryne
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68382	beta-galactosidase	+	3.2.1.23	from API zym
68379	beta-galactosidase	+	3.2.1.23	from API Coryne
68382	beta-glucosidase	+	3.2.1.21	from API zym
68379	beta-glucosidase	-	3.2.1.21	from API Coryne
68382	beta-glucuronidase	-	3.2.1.31	from API zym
68379	beta-glucuronidase	-	3.2.1.31	from API Coryne
30485	catalase	+	1.11.1.6
68382	esterase lipase (C 8)	-		from API zym
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68379	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API Coryne
68379	pyrazinamidase	-	3.5.1.B15	from API Coryne
68379	pyrrolidonyl arylamidase	-	3.4.19.3	from API Coryne
68382	trypsin	-	3.4.21.4	from API zym
68379	urease	-	3.5.1.5	from API Coryne
68382	valine arylamidase	+		from API zym

API coryne

@ref	Reduction of nitrateNIT	PYZ	PYRA	PAL	beta GUR	beta GAL	alpha GLU	beta NAG	ESC	URE	GEL	Control fermentationControl	GLU	RIB	XYL	MAN	MAL	LAC	SAC	GLYG	CAT
69381	-	-	-	+	-	+	+	-	-	-	+/-	not determinedn.d.	-	-	-	-	-	-	-	-	not determinedn.d.

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
69381	not determinedn.d.	+	+/-	-	-	+	+	+/-	-	+/-	+	+/-	-	+	-	+	+	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Aquatic	#Marine
#Environmental	#Aquatic	#Sediment

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent	Latitude	Longitude
16222	deep-sea sediment	Northern South China Sea (120° 0.975' E 19° 0.664' N) at a depth of 3258 m	China	CHN	Asia	6.6667	120.967 6.6667/120.967

Taxonmaps

69479

Global distribution of 16S sequence JN204514 (>99% sequence identity) for Pseudonocardia from Microbeatlas

Aquatic Samples	1601
Soil Samples	6624
Animal Samples	3592
Plant Samples	1889
Total Samples	13706

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
16222	1	Risk group (German classification) According to TRBA 466

Sequence information

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
16222	Pseudonocardia antitumoralis strain SCSIO 01299 16S ribosomal RNA gene, partial sequence	JN204514	1435		1070982

GC content

16222

GC-content (mol%)73.2

Genome-based predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
Pathogenicity	Small molecule deoxynyboquinone triggers alkylation and ubiquitination of Keap1 at Cys489 on Kelch domain for Nrf2 activation and inflammatory therapy.	Linghu KG, Zhang T, Zhang GT, Lv P, Zhang WJ, Zhao GD, Xiong SH, Ma QS, Zhao MM, Chen M, Hu YJ, Zhang CS, Yu H.	J Pharm Anal	10.1016/j.jpha.2023.07.009	2024
Genetics	Bacteriobiota of the Cave Church of Sts. Peter and Paul in Serbia-Culturable and Non-Culturable Communities' Assessment in the Bioconservation Potential of a Peculiar Fresco Painting.	Dimkic I, Copic M, Petrovic M, Stupar M, Savkovic Z, Knezevic A, Subakov Simic G, Ljaljevic Grbic M, Unkovic N.	Int J Mol Sci	10.3390/ijms24021016	2023
Enzymology	Functional Characterization of a Novel Marine Microbial Esterase and its Utilization in the Enantioselective Preparation of (R)-Methyl 2-Chloropropionate.	Cao Y, Deng D, Sun A, Zhang Y, Hu Y	Appl Biochem Biotechnol	10.1007/s12010-016-2094-8	2016
Phylogeny	Pseudonocardia tritici sp. nov., a novel actinomycete isolated from rhizosphere soil of wheat (Triticum aestivum L.).	Song J, Qiu S, Zhao J, Han C, Wang Y, Sun X, Jiang S, Wang X, Xiang W	Antonie Van Leeuwenhoek	10.1007/s10482-018-01210-2	2018
Phylogeny	Pseudonocardia antitumoralis sp. nov., a deoxynyboquinone-producing actinomycete isolated from a deep-sea sediment.	Tian XP, Long LJ, Li SM, Zhang J, Xu Y, He J, Li J, Wang FZ, Li WJ, Zhang CS, Zhang S	Int J Syst Evol Microbiol	10.1099/ijs.0.037135-0	2012

References

#16222	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 45322
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#30485	Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #26819 (see below)
#68379	Automatically annotated from API Coryne .
#68382	Automatically annotated from API zym .
#69381	Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Pseudonocardia antitumoralis (DSM 45322, CCTCC M2011255, SCSIO 01299)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Multicellular morphology

Pigmentation

Multimedia

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Spore formation

Compound production

Halophily

Observation

Metabolite utilization

Enzymes

API coryne

API zym

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

16S sequences

GC content

Genome-based predictions

Literature

Literature

References

BacDive

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