Ensifer garamanticus (LMG 24692, CIP 109916, ORS 1400)

Name: Ensifer garamanticus (LMG 24692, CIP 109916, ORS 1400)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 133536

Type strain

Culture col. no. LMG 24692 CIP 109916 ORS 1400

NCBI tax ID(s) 680247

Special Collections CIP Literature strains

Links

Ensifer garamanticus LMG 24692 is an aerobe, Gram-negative, rod-shaped prokaryote that was isolated from Plant, Root nodule, Argyrolobium uniflorum.

Gram-negative rod-shaped aerobe genome sequence 16S sequence

Name and taxonomic classification

SI-ID 368955

NBRC 107849,CIP 109916,LMG 24692

@ref 20215

Last LPSN update 2025-12-16 10:01:48

Domain Pseudomonadati

Phylum Pseudomonadota

Class Alphaproteobacteria

Order Hyphomicrobiales

Family Rhizobiaceae

Genus Ensifer

Species Ensifer garamanticus

Full scientific name Ensifer garamanticus Merabet et al. 2010

Synonyms (1)

Sinorhizobium garamanticum

BacDive ID	Other strains from **Ensifer garamanticus** (2)	Type strain
137015	E. garamanticus CIP 109848, LMG 24693, ORS 1401
137016	E. garamanticus CIP 109849, LMG 24694, STM 354

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Motility
29389	negative	rod-shaped
119535	negative	rod-shaped

Pigmentation

29389

Productionyes

Culture and growth conditions

Culture medium

@ref	Name	Growth	Composition	Medium link
37881	MEDIUM 705 - for Ensifer		Distilled water make up to (1.000 l);Sodium chloride (0.050 g);Magnesiumsulphate heptahydrate (0.200 g);Calcium chloride dihydrate (0.040 g);Agar (15.000 g);Yeast extract (1.000 g);Potassium di-hydrogen phosphate (0.500 g);Sodium glutamate (0.500 g);Ferri
119535	CIP Medium 705			Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
29389	positive	growth	28-40
37881	positive	growth	30	mesophilic

Culture pH

@ref	Ability	Type	PH	PH range
29389	positive	growth	07-12	alkaliphile

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance
29389	aerobe
119535	obligate aerobe

Spore formation

29389

Spore formationno

Halophily

@ref	Salt	Growth	Tested relation	Concentration
29389	NaCl	positive	growth	01-04 %

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
29389	15792 ChEBI	malonate	+	carbon source
119535	17632 ChEBI	nitrate	+	reduction
119535	16301 ChEBI	nitrite	-	reduction

Metabolite production

@ref	Chebi-ID	Metabolite	Production
119535	35581 ChEBI	indole

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68382	beta-galactosidase	+	3.2.1.23	from API zym
68382	beta-glucosidase	+	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
119535	catalase	+	1.11.1.6
68382	cystine arylamidase	+	3.4.11.3	from API zym
68382	esterase (C 4)	-		from API zym
68382	esterase lipase (C 8)	-		from API zym
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
68382	N-acetyl-beta-glucosaminidase	+	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
119535	oxidase	+
68382	trypsin	+	3.4.21.4	from API zym
119535	urease	-	3.5.1.5
68382	valine arylamidase	+		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
119535	-	+	-	-	-	+	+	+	+	-	+	+	-	+	-	+	+	+	-	-

Isolation, sampling and environmental information

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent	Isolation date
119535	Plant, Root nodule, Argyrolobium uniflorum	South	Tunisia	TUN	Africa	1993

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
119535	1	Risk group (French classification)

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	NCBI tax ID	Score
124043	ASM2989206v1 assembly for Sinorhizobium garamanticum LMG 24692	complete	GCA_029892065	680247.3	680247	98.04

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
124043	Ensifer garamanticus strain ORS 1400 16S ribosomal RNA gene, partial sequence.	AY500255	1419		680247

GC content

29389

GC-content (mol%)62.4

Genome-based predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
	aac(6')-Iaq, a novel aminoglycoside acetyltransferase gene identified from an animal isolate Brucella intermedia DW0551.	Lin N, Xu W, Huang D, Liu C, Lu J, Zhu M, Bao Q, Pan W.	Front Cell Infect Microbiol	10.3389/fcimb.2025.1551240	2025
Phylogeny	Taxonomic and symbiotic diversity of bacteria isolated from nodules of Acacia tortilis subsp. raddiana in arid soils of Tunisia.	Fterich A, Mahdhi M, Lafuente A, Pajuelo E, Caviedes MA, Rodriguez-Llorente ID, Mars M.	Can J Microbiol	10.1139/w2012-048	2012
	Deep-Sea Actinobacteria Mitigate Salinity Stress in Tomato Seedlings and Their Biosafety Testing.	Rangseekaew P, Barros-Rodriguez A, Pathom-Aree W, Manzanera M.	Plants (Basel)	10.3390/plants10081687	2021
	Characterization and Genomic Analysis of Fererhizobium litorale gen. nov., sp. nov., Isolated from the Sandy Sediments of the Sea of Japan Seashore.	Romanenko L, Otstavnykh N, Tanaka N, Kurilenko V, Svetashev V, Tekutyeva L, Mikhailov V, Isaeva M.	Microorganisms	10.3390/microorganisms11102385	2023
Phylogeny	Proposal of Ensifer psoraleae sp. nov., Ensifer sesbaniae sp. nov., Ensifer morelense comb. nov. and Ensifer americanum comb. nov.	Wang YC, Wang F, Hou BC, Wang ET, Chen WF, Sui XH, Chen WX, Li Y, Zhang YB.	Syst Appl Microbiol	10.1016/j.syapm.2013.05.001	2013
Phylogeny	Multilocus sequence analysis of root nodule isolates from Lotus arabicus (Senegal), Lotus creticus, Argyrolobium uniflorum and Medicago sativa (Tunisia) and description of Ensifer numidicus sp. nov. and Ensifer garamanticus sp. nov.	Merabet C, Martens M, Mahdhi M, Zakhia F, Sy A, Le Roux C, Domergue O, Coopman R, Bekki A, Mars M, Willems A, de Lajudie P	Int J Syst Evol Microbiol	10.1099/ijs.0.012088-0	2009

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#29389	Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #25796 (see below)
#37881	; Curators of the CIP;
#68382	Automatically annotated from API zym .
#119535	Collection of Institut Pasteur ; Curators of the CIP; CIP 109916
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Ensifer garamanticus (LMG 24692, CIP 109916, ORS 1400)

Name and taxonomic classification

Morphology

Cell morphology

Pigmentation

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Metabolite utilization

Metabolite production

Enzymes

API zym

Isolation, sampling and environmental information

Isolation

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_029892065

16S sequences

GC content

Genome-based predictions

Literature

Literature

References

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