Name: Microbulbifer echini AM134
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 133417

Type strain:

Species: Microbulbifer echini

Strain Designation: AM134

Culture col. no.: KACC 18258, JCM 30400

Strain history: J.-W. Bae; Dept. of Biol., Kyung Hee Univ., South Korea; AM134.

NCBI tax ID(s): 1529067 (species)

Section

Microbulbifer echini AM134 is a facultative aerobe, Gram-negative, rod-shaped bacterium that forms circular colonies and was isolated from gut of a purple sea urchin collected from the coastal water.

colony-forming
Gram-negative
rod-shaped
facultative aerobe
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Cellvibrionales*
	Family	*Microbulbiferaceae*
	Genus	*Microbulbifer*
	Species	**Microbulbifer echini**
	Full Scientific Name (LPSN)	Microbulbifer echini Lee et al. 2017

Information on morphological and physiological properties Morphology

[Ref.: #25262]	Gram stain	negative

[Ref.: #25262]	Cell length	3.3-5.5 µm

[Ref.: #25262]	Cell width	0.9-1.1 µm
[Ref.: #25262]	Cell width	0.6-2.7 µm

[Ref.: #25262]	Cell shape	rod-shaped
[Ref.: #25262]	Cell shape	coccus-shaped

[Ref.: #25262]	Motility	no

[Ref.: #25262]	Colony size	0.1-0.2 cm
[Ref.: #25262]	Colony color	ivory to pale brown
[Ref.: #25262]	Colony shape	circular
[Ref.: #25262]	Cultivation medium used	MA

Information on culture and growth conditions Culture and growth conditions

[Ref.: #25262]

Culture medium

Marine agar (MA)

[Ref.: #25262]

Culture medium growth

		Temperature
[Ref.: #25262]	growth	10-36 °C
[Ref.: #25262]	optimum	30 °C
[Ref.: #67770]	growth	30 °C

[Ref.: #25262]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #25262]			optimum	7
[Ref.: #25262]			growth	6.2-9.0

Information on physiology and metabolism Physiology and metabolism

[Ref.: #25262]

Oxygen tolerance

facultative aerobe

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #25262]		NaCl		growth	1-8	%(w/v)
[Ref.: #25262]		NaCl		optimum	2	%(w/v)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #25262]	amygdalin ChEBI	-	builds acid from
[Ref.: #25262]	arbutin ChEBI	-	builds acid from
[Ref.: #25262]	casein	+	hydrolysis
[Ref.: #25262]	cellobiose ChEBI	-	builds acid from
[Ref.: #25262]	D-arabinose ChEBI	-	builds acid from
[Ref.: #25262]	D-arabitol ChEBI	-	builds acid from
[Ref.: #25262]	D-fructose ChEBI	-	builds acid from
[Ref.: #25262]	D-fucose ChEBI	-	builds acid from
[Ref.: #25262]	D-galactose ChEBI	-	builds acid from
[Ref.: #25262]	D-glucose ChEBI	-	builds acid from
[Ref.: #25262]	D-lyxose ChEBI	-	builds acid from
[Ref.: #25262]	D-mannitol ChEBI	-	builds acid from
[Ref.: #25262]	D-mannose ChEBI	+	builds acid from
[Ref.: #25262]	D-ribose ChEBI	-	builds acid from
[Ref.: #25262]	D-sorbitol ChEBI	-	builds acid from
[Ref.: #25262]	D-tagatose ChEBI	-	builds acid from
[Ref.: #25262]	D-xylose ChEBI	-	builds acid from
[Ref.: #25262]	dna ChEBI	+	hydrolysis
[Ref.: #25262]	erythritol ChEBI	-	builds acid from
[Ref.: #25262]	esculin ChEBI	-	hydrolysis
[Ref.: #25262]	galactitol ChEBI	-	builds acid from
[Ref.: #25262]	gelatin ChEBI	+	hydrolysis
[Ref.: #25262]	gentiobiose ChEBI	-	builds acid from
[Ref.: #25262]	glucose ChEBI	-	fermentation
[Ref.: #25262]	glycerol ChEBI	-	builds acid from
[Ref.: #25262]	glycogen ChEBI	+	builds acid from
[Ref.: #25262]	inulin ChEBI	-	builds acid from
[Ref.: #25262]	L-arabinose ChEBI	-	builds acid from
[Ref.: #25262]	L-arabitol ChEBI	-	builds acid from
[Ref.: #25262]	L-fucose ChEBI	-	builds acid from
[Ref.: #25262]	L-rhamnose ChEBI	-	builds acid from
[Ref.: #25262]	L-sorbose ChEBI	-	builds acid from
[Ref.: #25262]	L-xylose ChEBI	-	builds acid from
[Ref.: #25262]	lactose ChEBI	-	builds acid from
[Ref.: #25262]	maltose ChEBI	+	builds acid from
[Ref.: #25262]	melezitose ChEBI	-	builds acid from
[Ref.: #25262]	melibiose ChEBI	-	builds acid from
[Ref.: #25262]	methyl alpha-D-glucopyranoside ChEBI	-	builds acid from
[Ref.: #25262]	methyl alpha-D-mannoside ChEBI	-	builds acid from
[Ref.: #25262]	methyl beta-D-xylopyranoside ChEBI	-	builds acid from
[Ref.: #25262]	myo-inositol ChEBI	-	builds acid from
[Ref.: #25262]	N-acetylglucosamine ChEBI	-	builds acid from
[Ref.: #25262]	nitrate ChEBI	+	reduction
[Ref.: #25262]	nitrite ChEBI	-	reduction
[Ref.: #25262]	potassium 2-dehydro-D-gluconate	-	builds acid from
[Ref.: #25262]	potassium 5-dehydro-D-gluconate	-	builds acid from
[Ref.: #25262]	potassium gluconate ChEBI	-	builds acid from
[Ref.: #25262]	raffinose ChEBI	-	builds acid from
[Ref.: #25262]	ribitol ChEBI	-	builds acid from
[Ref.: #25262]	salicin ChEBI	-	builds acid from
[Ref.: #25262]	starch ChEBI	-	builds acid from
[Ref.: #25262]	starch ChEBI	+	hydrolysis
[Ref.: #25262]	sucrose ChEBI	-	builds acid from
[Ref.: #25262]	trehalose ChEBI	+	builds acid from
[Ref.: #25262]	turanose ChEBI	-	builds acid from
[Ref.: #25262]	tween 20 ChEBI	-	hydrolysis
[Ref.: #25262]	tween 40 ChEBI	+	hydrolysis
[Ref.: #25262]	tween 60 ChEBI	+	hydrolysis
[Ref.: #25262]	tween 80 ChEBI	+	hydrolysis
[Ref.: #25262]	urea ChEBI	+	hydrolysis
[Ref.: #25262]	xylitol ChEBI	-	builds acid from
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Production
[Ref.: #25262]	acetoin ChEBI	no
[Ref.: #25262]	hydrogen sulfide ChEBI	no
[Ref.: #25262]	indole ChEBI	no

	Physiological tests	Metabolite	Voges-Proskauer-test	Methylred-test
[Ref.: #25262]		acetoin ChEBI	-
[Ref.: #25262]		glucose ChEBI		-

	Enzyme	Enzyme activity	EC number
[Ref.: #25262]	acid phosphatase	+	3.1.3.2
[Ref.: #25262]	alkaline phosphatase	+	3.1.3.1
[Ref.: #25262]	alpha-glucosidase	+	3.2.1.20
[Ref.: #25262]	catalase	+	1.11.1.6
[Ref.: #25262]	cytochrome oxidase	+	1.9.3.1
[Ref.: #25262]	esterase (C 4)	+
[Ref.: #25262]	esterase Lipase (C 8)	+
[Ref.: #25262]	gelatinase	+
[Ref.: #25262]	leucine arylamidase	+	3.4.11.1
[Ref.: #25262]	lipase (C 14)	+
[Ref.: #25262]	naphthol-AS-BI-phosphohydrolase	+
[Ref.: #25262]	trypsin	-	3.4.21.4
[Ref.: #25262]	valine arylamidase	+
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #25262]

Fatty acid	Percentage	ECL
C18:1ω6c / C18:1ω7c	19.4
C16:0	16.4
C14:0	11.2
cyclo C19:0ω8c	9.7
C12:0 3OH	6.6
cyclo C17:0	5.3
C12:0	3.2
C10:0 3OH	2.9
C15:0 iso	1.2
C17:1ω8c	0.8
C9:0	0.7
C16:0 3OH	0.6
C19:1ω6c / Unknown ECL 18.846	0.5	19
C17:0	0.5
C11:0 3OH	0.5
10-Methyl C19:0	0.5

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #25262]	Sample type/isolated from	gut of a purple sea urchin (Heliocidaris crassispina) collected from the coastal water
[Ref.: #25262]	Geographic location (country and/or sea, region)	Dokdo Island in Korea
[Ref.: #25262]	Country	Republic of Korea
[Ref.: #25262]	Country ISO 3 Code	KOR
[Ref.: #25262]	Continent	Asia

[Ref.: #67770]	Sample type/isolated from	Gut of a purple sea urchin, Heliocidaris crassispina
[Ref.: #67770]	Host species	Heliocidaris crassispina
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	-
#Host	#Invertebrates (Other)	#Echinodermata
#Host Body-Site	#Gastrointestinal tract	#Stomach

What are isolation sources categories?

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #25262]	16S rRNA gene sequences	KJ789957	1455	ENA	1529067 tax ID

[Ref.: #25262]	GC-content	56.1 mol%	Thermal denaturation, fluorometry
[Ref.: #67770]	GC-content	56.1 mol%	thermal denaturation, midpoint method (Tm)

Availability in culture collections External links

[Ref.: #25262]

Culture collection no.

KACC 18258, JCM 30400

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Microbulbifer echini sp. nov., isolated from the gastrointestinal tract of a purple sea urchin, Heliocidaris crassispina.	Lee JY, Kim PS, Hyun DW, Kim HS, Shin NR, Jung MJ, Yun JH, Kim MS, Whon TW, Bae JW	Int J Syst Evol Microbiol	10.1099/ijsem.0.001731	2017	*
Phylogeny	Microbulbifer okhotskensis sp. nov., isolated from a deep bottom sediment of the Okhotsk Sea.	Romanenko L, Kurilenko V, Otstavnykh N, Velansky P, Isaeva M, Mikhailov V	Arch Microbiol	10.1007/s00203-022-03161-9	2022	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#25262

J. Y. K. Lee, P. S.,Hyun, D. W.,Kim, H. S.,Shin, N. R.,Jung, M. J.,Yun, J. H.,Kim, M. S.,Whon, T. W.,Bae, J. W.: Microbulbifer echini sp. nov., isolated from the gastrointestinal tract of a purple sea urchin, Heliocidaris crassispina. IJSEM 67: 998 - 1004 2017 ( DOI 10.1099/ijsem.0.001731 , PubMed 27959777 )

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;
* These data were automatically processed and therefore are not curated

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