Name: Chryseobacterium nepalense C-5-3
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 133365

Type strain:

Species: Chryseobacterium nepalense

Strain Designation: C-5-3

Culture col. no.: KEMB 9005-411, KACC 18907, JCM 31469

Strain history: J. Kim; Kyonggi Univ., South Korea; C-5-3.

NCBI tax ID(s): 1854498 (species)

Special Collections

Section

When using BacDive for research please cite our paper

Chryseobacterium nepalense C-5-3 is an aerobe, Gram-negative, rod-shaped bacterium that forms circular colonies and was isolated from isolated from oil-contaminated soil of Biratnagar.

colony-forming
Gram-negative
rod-shaped
aerobe
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	09-12-2024 (DD-MM-YYYY)
	Domain	*Bacteria*
	Phylum	*Bacteroidota*
	Class	*Flavobacteriia*
	Order	*Flavobacteriales*
	Family	*Weeksellaceae*
	Genus	*Chryseobacterium*
	Species	**Chryseobacterium nepalense**
	Full Scientific Name (LPSN)	Chryseobacterium nepalense Chaudhary and Kim 2017

Information on morphological and physiological properties Morphology

[Ref.: #25219]	Gram stain	negative

[Ref.: #25219]	Cell length	1.2-1.8 µm

[Ref.: #25219]	Cell width	0.6-0.7 µm

[Ref.: #25219]	Cell shape	rod-shaped

[Ref.: #25219]	Motility	no

[Ref.: #25219]	Colony size	2-4 mm
[Ref.: #25219]	Colony color	yellow
[Ref.: #25219]	Colony shape	circular
[Ref.: #25219]	Incubation period	3 days
[Ref.: #25219]	Cultivation medium used	R2A

[Ref.: #25219]	Pigment production	yes
[Ref.: #25219]	Pigment name	Flexirubin-type

Information on culture and growth conditions Culture and growth conditions

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Culture medium

Reasoner's 2A agar (R2A)

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Culture medium growth

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Culture medium

Trypticase Soy Agar (TSA)

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Culture medium growth

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Culture medium

LB (Luria-Bertani) MEDIUM

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Culture medium growth

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Culture medium

Marine agar (MA)

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Culture medium growth

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Culture medium

brain heart infusion agar

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Culture medium growth

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Culture medium

Nutrient agar (NA)

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Culture medium growth

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Culture medium

veal infusion agar

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Culture medium growth

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Culture medium

MacConkey agar

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Culture medium growth

		Temperature
[Ref.: #25219]	growth	10-45 °C
[Ref.: #25219]	optimum	20-40 °C
[Ref.: #67770]	growth	30 °C

	pH	Kind of pH		pH
[Ref.: #25219]			optimum	6.5-9.0
[Ref.: #25219]			growth	6.0-11.0

Information on physiology and metabolism Physiology and metabolism

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Oxygen tolerance

aerobe

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Ability of spore formation

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #25219]		NaCl		growth	0	%

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Observation

quinones: MK-6

[Ref.: #25219]	Name of tolerated compound	NaCl
[Ref.: #25219]	Tolerance percentage	0-4

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #25219]	3-hydroxybenzoate ChEBI	-	assimilation
[Ref.: #25219]	3-hydroxybutyrate ChEBI	-	assimilation
[Ref.: #25219]	4-hydroxybenzoate ChEBI	-	assimilation
[Ref.: #25219]	4-nitrophenyl beta-D-galactopyranoside ChEBI	+	assimilation
[Ref.: #25219]	adipate ChEBI	-	assimilation
[Ref.: #25219]	carboxymethylcellulose ChEBI	+	hydrolysis
[Ref.: #25219]	casein	-	hydrolysis
[Ref.: #25219]	chitin ChEBI	-	hydrolysis
[Ref.: #25219]	D-glucose ChEBI	+	assimilation
[Ref.: #25219]	D-mannitol ChEBI	-	assimilation
[Ref.: #25219]	D-mannose ChEBI	-	assimilation
[Ref.: #25219]	D-ribose ChEBI	-	assimilation
[Ref.: #25219]	D-sorbitol ChEBI	-	assimilation
[Ref.: #25219]	decanoate ChEBI	-	assimilation
[Ref.: #25219]	dna ChEBI	+	hydrolysis
[Ref.: #25219]	esculin ChEBI	+	hydrolysis
[Ref.: #25219]	esculin ferric citrate	+	assimilation
[Ref.: #25219]	gelatin ChEBI	+	hydrolysis
[Ref.: #25219]	gelatin ChEBI	+	assimilation
[Ref.: #25219]	glucose ChEBI	-	fermentation
[Ref.: #25219]	glycogen ChEBI	+	assimilation
[Ref.: #25219]	hypoxanthine ChEBI	-	hydrolysis
[Ref.: #25219]	itaconate ChEBI	-	assimilation
[Ref.: #25219]	L-alanine ChEBI	-	assimilation
[Ref.: #25219]	L-arabinose ChEBI	+	assimilation
[Ref.: #25219]	L-arginine ChEBI	-	fermentation
[Ref.: #25219]	L-fucose ChEBI	-	assimilation
[Ref.: #25219]	L-histidine ChEBI	+	assimilation
[Ref.: #25219]	L-proline ChEBI	+/-	assimilation
[Ref.: #25219]	L-rhamnose ChEBI	+	assimilation
[Ref.: #25219]	L-serine ChEBI	-	assimilation
[Ref.: #25219]	L-tryptophan ChEBI	+	assimilation
[Ref.: #25219]	lactate ChEBI	-	assimilation
[Ref.: #25219]	malate ChEBI	-	assimilation
[Ref.: #25219]	maltose ChEBI	+	assimilation
[Ref.: #25219]	melibiose ChEBI	-	assimilation
[Ref.: #25219]	myo-inositol ChEBI	-	assimilation
[Ref.: #25219]	N-acetylglucosamine ChEBI	-	assimilation
[Ref.: #25219]	nitrate ChEBI	-	reduction
[Ref.: #25219]	phenylacetate ChEBI	-	assimilation
[Ref.: #25219]	potassium 2-dehydro-D-gluconate	-	assimilation
[Ref.: #25219]	potassium 5-dehydro-D-gluconate	-	assimilation
[Ref.: #25219]	potassium gluconate ChEBI	-	assimilation
[Ref.: #25219]	propionate ChEBI	-	assimilation
[Ref.: #25219]	salicin ChEBI	+	assimilation
[Ref.: #25219]	sodium acetate ChEBI	-	assimilation
[Ref.: #25219]	sodium citrate ChEBI	-	assimilation
[Ref.: #25219]	sodium malonate ChEBI	-	assimilation
[Ref.: #25219]	starch ChEBI	+	hydrolysis
[Ref.: #25219]	suberic acid ChEBI	-	assimilation
[Ref.: #25219]	sucrose ChEBI	-	assimilation
[Ref.: #25219]	tween 40 ChEBI	-	hydrolysis
[Ref.: #25219]	tween 80 ChEBI	+	hydrolysis
[Ref.: #25219]	tyrosine ChEBI	-	hydrolysis
[Ref.: #25219]	urea ChEBI	-	fermentation
[Ref.: #25219]	valerate ChEBI	-	assimilation
[Ref.: #25219]	xanthine ChEBI	-	hydrolysis
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Antibiotic sensitivity	Concentration		Antibiotic resistance	Concentration
[Ref.: #25219]	ampicillin ChEBI				yes	30	µg
[Ref.: #25219]	chloramphenicol ChEBI				yes	100	µg
[Ref.: #25219]	cycloheximide ChEBI				yes	30	µg
[Ref.: #25219]	gentamicin ChEBI	yes	10	µg
[Ref.: #25219]	kanamycin ChEBI	yes	30	µg
[Ref.: #25219]	nalidixic acid ChEBI	yes	30	µg
[Ref.: #25219]	neomycin ChEBI				yes	30	µg
[Ref.: #25219]	novobiocin ChEBI	yes	30	µg
[Ref.: #25219]	penicillin ChEBI				yes	10	µg
[Ref.: #25219]	rifampicin ChEBI	yes	10	µg
[Ref.: #25219]	streptomycin ChEBI	yes	10	µg
[Ref.: #25219]	sulfamethoxazole ChEBI				yes	30	µg
[Ref.: #25219]	tetracycline ChEBI	yes	30	µg
[Ref.: #25219]	trimethoprim ChEBI	yes	30	µg
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #25219]		hydrogen sulfide ChEBI	no
[Ref.: #25219]		indole ChEBI	yes

	Enzyme	Enzyme activity	EC number
[Ref.: #25219]	acid phosphatase	+	3.1.3.2
[Ref.: #25219]	alkaline phosphatase	+	3.1.3.1
[Ref.: #25219]	alpha-chymotrypsin	-	3.4.21.1
[Ref.: #25219]	alpha-fucosidase	-	3.2.1.51
[Ref.: #25219]	alpha-galactosidase	-	3.2.1.22
[Ref.: #25219]	alpha-glucosidase	+	3.2.1.20
[Ref.: #25219]	alpha-mannosidase	-	3.2.1.24
[Ref.: #25219]	beta-galactosidase	-	3.2.1.23
[Ref.: #25219]	beta-glucosidase	+	3.2.1.21
[Ref.: #25219]	beta-glucuronidase	-	3.2.1.31
[Ref.: #25219]	catalase	+	1.11.1.6
[Ref.: #25219]	cystine arylamidase	+/-	3.4.11.3
[Ref.: #25219]	cytochrome oxidase	+	1.9.3.1
[Ref.: #25219]	esterase (C 4)	+
[Ref.: #25219]	esterase Lipase (C 8)	+
[Ref.: #25219]	leucine arylamidase	+	3.4.11.1
[Ref.: #25219]	lipase (C 14)	-
[Ref.: #25219]	N-acetyl-beta-glucosaminidase	+	3.2.1.52
[Ref.: #25219]	naphthol-AS-BI-phosphohydrolase	+
[Ref.: #25219]	trypsin	-	3.4.21.4
[Ref.: #25219]	valine arylamidase	+
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #25219]

Fatty acid	Percentage
C15:0 iso	29.4
C16:1ω7c / C16:1ω6c	20.68
C17:0 iso 3OH	17.66
C17:1 iso ω9c / C16:0 10-methyl	16.59

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #25219]	Sample type/isolated from	isolated from oil-contaminated soil of Biratnagar
[Ref.: #25219]	Geographic location (country and/or sea, region)	Biratnagar, Morang, Nepal
[Ref.: #25219]	Country	Nepal
[Ref.: #25219]	Country ISO 3 Code	NPL
[Ref.: #25219]	Continent	Asia
[Ref.: #25219]	Geographic location	26.0000°/87.0000°

[Ref.: #67770]	Sample type/isolated from	Oil-contaminated soil of Biratnagar
[Ref.: #67770]	Geographic location (country and/or sea, region)	Morang
[Ref.: #67770]	Country	Nepal
[Ref.: #67770]	Country ISO 3 Code	NPL
[Ref.: #67770]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Engineered	#Contamination	#Oil (Fuel)
#Environmental	#Terrestrial	#Soil

What are isolation sources categories?

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #25219]	Chryseobacterium nepalense strain C-5-3 16S ribosomal RNA gene, partial sequence	KX129820	1458	GenBank ENA	1854498 tax ID

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GC-content

38.6 mol%

high performance liquid chromatography (HPLC)

Availability in culture collections External links

[Ref.: #25219]

Culture collection no.

KEMB 9005-411, KACC 18907, JCM 31469

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Chryseobacterium cheonjiense sp. nov., isolated from forest soil.	Chaudhary DK, Dahal RH, Kim DU, Kim J	Arch Microbiol	10.1007/s00203-020-02065-w	2020	*
Phylogeny	Chryseobacterium nepalense sp. nov., isolated from oil-contaminated soil.	Chaudhary DK, Kim J	Int J Syst Evol Microbiol	10.1099/ijsem.0.001680	2017	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#25219

D. K. K. Chaudhary, J.: Chryseobacterium nepalense sp. nov., isolated from oil-contaminated soil. IJSEM 67: 646 - 652 2017 ( DOI 10.1099/ijsem.0.001680 , PubMed 27902299 )

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;
* These data were automatically processed and therefore are not curated

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