Prauserella halophila (DSM 44617, YIM 90001, KCTC 19000)

Name: Prauserella halophila (DSM 44617, YIM 90001, KCTC 19000)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 13316

Type strain

Culture col. no. DSM 44617 YIM 90001 KCTC 19000 CIP 107978 CCTCC AA 001015 2 more

NCBI tax ID(s) 185641

Special Collections DSMZ JCM KCTC CIP Wink compendium

Links

Prauserella halophila DSM 44617 is a mesophilic, Gram-positive, rod-shaped prokaryote that was isolated from soil in hypersaline habitat.

Gram-positive rod-shaped mesophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 128007

DSM 44617,BCRC 16311,CCRC 16311,JCM 13023,CIP 107978,KCTC 19000

@ref 20215

Last LPSN update 2025-12-16 09:50:03

Domain Bacillati

Phylum Actinomycetota

Class Actinomycetes

Order Pseudonocardiales

Family Pseudonocardiaceae

Genus Prauserella

Species Prauserella halophila

Full scientific name Prauserella halophila Li et al. 2003

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Motility
119809	positive	rod-shaped
125439	positive

Colony morphology

@ref	Colony color	Incubation period	Medium used
19683	Beige	10-14 days	ISP 2
19683	Beige	10-14 days	ISP 3
19683	Beige	10-14 days	ISP 4
19683	Beige	10-14 days	ISP 5
19683	Beige	10-14 days	ISP 6
19683	Beige	10-14 days	ISP 7

Multicellular morphology

@ref	Forms multicellular complex	Medium name
19683		ISP 2
19683		ISP 3
19683		ISP 4
19683		ISP 5
19683		ISP 6
19683		ISP 7

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
11930	GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)	Medium recipe at MediaDive	Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65; with strain-specific modifications) Composition: NaCl 100.0 g/l Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
19683	ISP 2		Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19683	ISP 3		Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19683	ISP 4		Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19683	ISP 5		Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
19683	ISP 6		Name: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
19683	ISP 7		Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
33186	Marine agar (MA)		Distilled water make up to (1000.000 ml);Marine agar (55.100 g)
119809	CIP Medium 13	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
11930	positive	growth	28	mesophilic
19683	positive	optimum	28	mesophilic
33186	positive	growth	30	mesophilic
67770	positive	growth	28	mesophilic
119809	positive	growth	25-37	mesophilic
119809	negative	growth	10
119809	negative	growth	15
119809	negative	growth	41
119809	negative	growth	45

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
125439	obligate aerobe	92.6

Halophily

@ref	Salt	Growth	Tested relation	Concentration
119809	NaCl	positive	growth	6-10 %
119809	NaCl		growth	0 %
119809	NaCl		growth	2 %
119809	NaCl		growth	4 %

Observation

67770

Observationquinones: MK-9(H₄)

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity
19683	22599 ChEBI	arabinose	-
19683	62968 ChEBI	cellulose	-
119809	16947 ChEBI	citrate	-
119809	4853 ChEBI	esculin	-
19683	28757 ChEBI	fructose	+
19683	17234 ChEBI	glucose	-
19683	29864 ChEBI	mannitol	+
19683	17268 ChEBI	myo-inositol	-
119809	17632 ChEBI	nitrate	-
119809	16301 ChEBI	nitrite	-
19683	16634 ChEBI	raffinose	-
19683	26546 ChEBI	rhamnose	-
19683	17992 ChEBI	sucrose	+
19683	18222 ChEBI	xylose	-

Metabolite production

@ref	Chebi-ID	Metabolite	Production
119809	35581 ChEBI	indole

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test
119809	15688 ChEBI	acetoin	+
119809	17234 ChEBI	glucose

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	-	3.1.3.2	from API zym
119809	alcohol dehydrogenase	-	1.1.1.1
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	-	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68382	beta-galactosidase	-	3.2.1.23	from API zym
119809	beta-galactosidase	-	3.2.1.23
68382	beta-glucosidase	-	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
119809	catalase	+	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
119809	gamma-glutamyltransferase	-	2.3.2.2
119809	gelatinase	-
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
119809	lysine decarboxylase	-	4.1.1.18
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
119809	ornithine decarboxylase	-	4.1.1.17
119809	oxidase	-
119809	phenylalanine ammonia-lyase	-	4.3.1.24
68382	trypsin	-	3.4.21.4	from API zym
119809	tryptophan deaminase	-
119809	urease	-	3.5.1.5
68382	valine arylamidase	-		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
19683	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	-	not determinedn.d.	not determinedn.d.	-	-	-	not determinedn.d.	-	-	-	-	not determinedn.d.	not determinedn.d.	-	-	-
119809	-	+	+	+	-	+	-	-	-	-	-	+	-	-	-	-	-	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Terrestrial	#Soil
#Condition	#Saline	-

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
11930	soil in hypersaline habitat	Lingiang province	China	CHN	Asia
67770	Soil from hypersaline habitats	Xinjang Province	China	CHN	Asia
119809	Environment, Soil, hypersaline	Xinjiang	China	CHN	Asia

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
11930	1	Risk group (German classification) According to TRBA 466
19683	1	Risk group (German classification) According to TRBA 466
119809	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM2417189v1 assembly for Prauserella halophila DSM 44617	scaffold	GCA_024171895	185641.3	2596583595	185641	74.79
124043	ASM3952829v1 assembly for Prauserella halophila JCM 13023	scaffold	GCA_039528295			185641	41.27

Genome browser: GCA_024171895

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
20218	Prauserella halophila strain YIM 90001 16S ribosomal RNA gene, partial sequence	AF466190	1514		185641

GC content

@ref	GC-content (mol%)	Method
67770	69.8	genome sequence analysis
67770	65.8	thermal denaturation, midpoint method (Tm)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Prauserella halophila DSM 44617
NCBI: GCA_024171895

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	57.10	no
125439	motility	BacteriaNetⓘ	no	68.70	no
125439	gram_stain	BacteriaNetⓘ	positive	96.90	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	92.60	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Genetics	Genome-Based Taxonomic Classification of the Phylum Actinobacteria.	Nouioui I, Carro L, Garcia-Lopez M, Meier-Kolthoff JP, Woyke T, Kyrpides NC, Pukall R, Klenk HP, Goodfellow M, Goker M.	Front Microbiol	10.3389/fmicb.2018.02007	2018
Phylogeny	Prauserella muralis sp. nov., from the indoor environment.	Schafer J, Martin K, Kampfer P	Int J Syst Evol Microbiol	10.1099/ijs.0.012005-0	2009
Phylogeny	Prauserella halophila sp. nov. and Prauserella alba sp. nov., moderately halophilic actinomycetes from saline soil.	Li WJ, Xu P, Tang SK, Xu LH, Kroppenstedt RM, Stackebrandt E, Jiang CL	Int J Syst Evol Microbiol	10.1099/ijs.0.02611-0	2003

References

#11930	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 44617
#19683	Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#33186	; Curators of the CIP;
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68382	Automatically annotated from API zym .
#119809	Collection of Institut Pasteur ; Curators of the CIP; CIP 107978
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Prauserella halophila (DSM 44617, YIM 90001, KCTC 19000)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Multicellular morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Halophily

Observation

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

API zym

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_024171895

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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