Name: Oceanobacillus longus T9B
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 133157

Type strain:

Species: Oceanobacillus longus

Strain Designation: T9B

Culture col. no.: IBRC-M 10703, LMG 29250

NCBI tax ID(s): 930120 (species)

Section

Oceanobacillus longus T9B is an obligate aerobe, moderately halophilic, spore-forming bacterium that forms circular colonies and was isolated from water sample .

colony-forming
Gram-positive
motile
rod-shaped
spore-forming
moderately halophilic
obligate aerobe
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Bacillaceae*
	Genus	*Oceanobacillus*
	Species	**Oceanobacillus longus**
	Full Scientific Name (LPSN)	Oceanobacillus longus Amoozegar et al. 2016

Information on morphological and physiological properties Morphology

[Ref.: #24931]	Gram stain	positive

[Ref.: #24931]	Cell length	4.0-20.0 µm

[Ref.: #24931]	Cell width	1 µm

[Ref.: #24931]	Cell shape	rod-shaped

[Ref.: #24931]	Motility	yes

[Ref.: #24931]	Flagellum arrangement	peritrichous

[Ref.: #24931]	Colony size	1.7-1.9 mm
[Ref.: #24931]	Colony color	brown
[Ref.: #24931]	Colony shape	circular
[Ref.: #24931]	Incubation period	2 days
[Ref.: #24931]	Cultivation medium used	7.5% HM agar

Information on culture and growth conditions Culture and growth conditions

[Ref.: #24931]

Culture medium

7.5% HM medium

[Ref.: #24931]

Culture medium growth

[Ref.: #24931]

Culture medium

7.5% HM agar

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Culture medium growth

	Temperatures	Kind of temperature		Temperature
[Ref.: #24931]			growth	25.0-45.0 °C
[Ref.: #24931]			optimum	35 °C

[Ref.: #24931]

Temperature range

mesophilic

	pH	Kind of pH		pH
[Ref.: #24931]			optimum	7
[Ref.: #24931]			growth	6.0-9.0

Information on physiology and metabolism Physiology and metabolism

[Ref.: #24931]

Oxygen tolerance

obligate aerobe

[Ref.: #24931]	Spore description	subterminal or central ellipsoidal,in swollen sporangia
[Ref.: #24931]	Type of spore	endospore
[Ref.: #24931]	Ability of spore formation	yes

[Ref.: #24931]

Halophily / tolerance level

moderately halophilic

	Salt	Tested relation		Salt conc.
[Ref.: #24931]	NaCl		growth	1.0-20.0	%(w/v)
[Ref.: #24931]	NaCl	no	growth	0	%(w/v)
[Ref.: #24931]	NaCl		optimum	5	%(w/v)

[Ref.: #24931]	Murein short key	A31
[Ref.: #24931]	Murein types	A1gamma m-Dpm-direct

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #24931]	ammonium ChEBI	+	nitrogen source
[Ref.: #24931]	casein	-	hydrolysis
[Ref.: #24931]	citrate ChEBI	+	carbon source
[Ref.: #24931]	D-fructose ChEBI	+	builds acid from
[Ref.: #24931]	D-galactose ChEBI	-	builds acid from
[Ref.: #24931]	D-glucose ChEBI	+	builds acid from
[Ref.: #24931]	D-mannitol ChEBI	-	builds acid from
[Ref.: #24931]	D-ribose ChEBI	-	builds acid from
[Ref.: #24931]	D-xylose ChEBI	-	builds acid from
[Ref.: #24931]	dna ChEBI	-	hydrolysis
[Ref.: #24931]	esculin ChEBI	-	hydrolysis
[Ref.: #24931]	gelatin ChEBI	+	hydrolysis
[Ref.: #24931]	glucose ChEBI	-	fermentation
[Ref.: #24931]	lactose ChEBI	-	builds acid from
[Ref.: #24931]	nitrate ChEBI	-	reduction
[Ref.: #24931]	nitrite ChEBI	-	reduction
[Ref.: #24931]	starch ChEBI	-	hydrolysis
[Ref.: #24931]	sucrose ChEBI	-	builds acid from
[Ref.: #24931]	tween 20 ChEBI	+	hydrolysis
[Ref.: #24931]	tween 40 ChEBI	+	hydrolysis
[Ref.: #24931]	tween 60 ChEBI	+	hydrolysis
[Ref.: #24931]	tween 80 ChEBI	+	hydrolysis
[Ref.: #24931]	urea ChEBI	-	hydrolysis
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Production
[Ref.: #24931]	acetoin ChEBI	no
[Ref.: #24931]	hydrogen sulfide ChEBI	no
[Ref.: #24931]	indole ChEBI	no

	Metabolite	Voges-Proskauer-test	Methylred-test	Citrate test
[Ref.: #24931]	acetoin ChEBI	-
[Ref.: #24931]	citrate ChEBI			+
[Ref.: #24931]	glucose ChEBI		-

	Enzyme	Enzyme activity	EC number
[Ref.: #24931]	arginine dihydrolase	-	3.5.3.6
[Ref.: #24931]	catalase	+	1.11.1.6
[Ref.: #24931]	cytochrome oxidase	+	1.9.3.1
[Ref.: #24931]	lysine decarboxylase	-	4.1.1.18
[Ref.: #24931]	ornithine decarboxylase	-	4.1.1.17

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #24931]	Sample type/isolated from	water sample (pH 7.1,salinity 31 %)
[Ref.: #24931]	Geographic location (country and/or sea, region)	Aran-Bidgol salt lake, the largest playa in the centre of Iran
[Ref.: #24931]	Country	Iran
[Ref.: #24931]	Country ISO 3 Code	IRN
[Ref.: #24931]	Continent	Asia
[Ref.: #24931]	Enrichment culture	7.5% HM medium
[Ref.: #24931]	Enrichment culture composition	containing (g l-1): NaCl, 60.75; MgCl2.6H2O,5.25; MgSO4.7H2O, 7.2; CaCl2.2H2O, 0.27; KCl, 1.5;NaHCO3 , 0.045; NaBr, 0.0195; proteose-peptone no. 3, 5; yeast extract, 10; and glucose, 1; solidified with 1.5 % agar
[Ref.: #24931]	Enrichment culture temperature	35 ̊C
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	-
#Condition	#Saline	-

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence HQ433443 (>99% sequence identity) for Oceanobacillus longus subclade from Microbeatlas

Aquatic Samples	8
Soil Samples	13
Animal Samples	24
Plant Samples	1
Total Samples	46

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #24931]	Oceanobacillus longus strain T9B 16S ribosomal RNA gene, partial sequence	HQ433443	1484	GenBank	930120 tax ID

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GC-content

42.9 mol%

high performance liquid chromatography (HPLC)

Availability in culture collections External links

[Ref.: #24931]

Culture collection no.

IBRC-M 10703, LMG 29250

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Oceanobacillus longus sp. nov., a moderately halophilic bacterium isolated from a salt lake.	Amoozegar MA, Bagheri M, Makhdoumi A, Mehrshad M, Didari M, Schumann P, Sproer C, Sanchez-Porro C, Ventosa A	Int J Syst Evol Microbiol	10.1099/ijsem.0.001339	2016	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#24931

M. A. B. Amoozegar, Maryam,Makhdoumi, Ali,Mehrshad, Maliheh,Didari, Maryam,Schumann, Peter,Spröer, Cathrin,Sánchez-Porro, Cristina,Ventosa, Antonio: Oceanobacillus longus sp. nov., a moderately halophilic bacterium isolated from a salt lake. IJSEM 66: 4225 - 4230 2016 ( DOI 10.1099/ijsem.0.001339 , PubMed 27470165 )

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

* These data were automatically processed and therefore are not curated

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