Name: Lentzea flaviverrucosa AS4.0578
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 13312

Type strain:

Species: Lentzea flaviverrucosa

Strain Designation: AS4.0578

Culture col. no.: DSM 44664, AS 4.0578, JCM 11373, BCRC 16960, CGMCC 4.0578, CIP 107743, NBRC 100042

Strain history: CIP <- 2002, Z. Liu, Chinese Academy Sciences, Beijing, China: strain AS4.0578 <- CGMCC <- JCM <- Z. Liu

NCBI tax ID(s): 200379 (species)

SI-ID 100722

AS 4.0578, JCM 11373, DSM 44664, NBRC 100042, CIP 107743, CGMCC 4.0578

Section

Lentzea flaviverrucosa AS4.0578 is an obligate aerobe, spore-forming, mesophilic bacterium that was isolated from soil.

spore-forming
mesophilic
obligate aerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Pseudonocardiales*
	Family	*Pseudonocardiaceae*
	Genus	*Lentzea*
	Species	**Lentzea flaviverrucosa**
	Full Scientific Name (LPSN)	Lentzea flaviverrucosa (ex Yan and Deng 1966) Xie et al. 2002

Information on morphological and physiological properties Morphology

[Ref.: #119380]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #119380]	Cell shape	rod-shaped

[Ref.: #119380]	Motility	no
[Ref.: #69480]	Motility	no Confidence in %91.048

Information on culture and growth conditions Culture and growth conditions

[Ref.: #11860]

Culture medium

GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)

[Ref.: #11860]

Culture medium growth

[Ref.: #11860]

Culture medium link

Medium recipe at MediaDive

[Ref.: #11860]

Culture medium composition

expand / minimize

[Ref.: #39933]

Culture medium

MEDIUM 275 - for Actinomadura rugatobispora and Actinomadura verrucosospora

[Ref.: #39933]

Culture medium growth

[Ref.: #39933]

Culture medium composition

expand / minimize

[Ref.: #119380]

Culture medium

CIP Medium 275

[Ref.: #119380]

Culture medium growth

[Ref.: #119380]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #11860]		growth	28 °C
[Ref.: #39933]		growth	30 °C
[Ref.: #67770]		growth	28 °C
[Ref.: #119380]		growth	22-37 °C
[Ref.: #119380]	no	growth	10 °C
[Ref.: #119380]	no	growth	15 °C
[Ref.: #119380]	no	growth	45 °C

[Ref.: #11860]	Temperature range	mesophilic
[Ref.: #39933]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic
[Ref.: #119380]	Temperature range	psychrophilic
[Ref.: #119380]	Temperature range	thermophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #119380]

Oxygen tolerance

obligate aerobe

[Ref.: #69481]	Ability of spore formation	yes Confidence in %94
[Ref.: #69480]	Ability of spore formation	yes Confidence in %100

[Ref.: #67770]

Observation

quinones: MK-9(H₄), MK-9(H₂)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #119380]	nitrate ChEBI	-	reduction
[Ref.: #119380]	nitrate ChEBI	-	respiration
[Ref.: #119380]	nitrite ChEBI	-	reduction

	Antibiotica	Metabolite	Antibiotic sensitivity	Antibiotic resistance
[Ref.: #119380]		0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)	no	yes

	Physiological tests	Metabolite	Voges-Proskauer-test
[Ref.: #119380]		acetoin ChEBI	-

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #119380]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	+	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	+	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	+	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #119380]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #119380]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	+	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	-		* from API zym
[Ref.: #119380]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #119380]	oxidase	-
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #119380]	10616	-	+	+	+	-	+	-	-	-	-	+	-	+	+	-	+	-	+	+	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #11860]	Sample type/isolated from	soil
[Ref.: #11860]	Geographic location (country and/or sea, region)	Shanxi province, Wutaishan Mountain
[Ref.: #11860]	Country	China
[Ref.: #11860]	Country ISO 3 Code	CHN
[Ref.: #11860]	Continent	Asia

[Ref.: #67770]	Sample type/isolated from	Soil
[Ref.: #67770]	Geographic location (country and/or sea, region)	Wutaishan Mountain, Shanxi Province
[Ref.: #67770]	Country	China
[Ref.: #67770]	Country ISO 3 Code	CHN
[Ref.: #67770]	Continent	Asia

[Ref.: #119380]	Sample type/isolated from	Environment, Soil
[Ref.: #119380]	Geographic location (country and/or sea, region)	Wutaishan mountain
[Ref.: #119380]	Country	China
[Ref.: #119380]	Country ISO 3 Code	CHN
[Ref.: #119380]	Continent	Asia
[Ref.: #119380]	Isolation date	1966
* marker position based on {}

Isolation sources categories

#Environmental

#Terrestrial

#Soil

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AF183957 (>99% sequence identity) for Lentzea from Microbeatlas

Aquatic Samples	15
Soil Samples	231
Animal Samples	10
Plant Samples	33
Total Samples	289

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #11860]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #119380]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #11860]	Lentzea flavoverrucosus 16S ribosomal RNA gene, partial sequence	AF183957	1426	ENA	200379 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Lentzea flaviverrucosa CGMCC 4.578	GCA_900110985	scaffold	GenBank	200379 tax ID
[Ref.: #67770]	Lentzea flaviverrucosa DSM 44664	GCA_003350445	scaffold	GenBank	200379 tax ID
[Ref.: #66792]	Lentzea flaviverrucosa strain DSM 44664	200379.4	wgs	PATRIC	200379 tax ID	*
[Ref.: #66792]	Lentzea flaviverrucosa DSM 44664	2770939509	draft	JGI IMG/M	200379 tax ID	*

[Ref.: #11860]

GC-content

64.1 mol%

thermal denaturation, midpoint method (Tm)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Lentzea flaviverrucosa DSM 44664 NCBI: GCA_003350445.1
[Ref.: #69481]	spore-forming	yes	94	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Lentzea flaviverrucosa strain DSM 44664 PATRIC: 200379.4
[Ref.: #69480]	motile	no	91.755	no
[Ref.: #69480]	gram-positive	yes	89.845	no
[Ref.: #69480]	anaerobic	no	99.21	no
[Ref.: #69480]	aerobic	yes	92.204	no
[Ref.: #69480]	halophile	no	85.418	no
[Ref.: #69480]	spore-forming	yes	93.648	no
[Ref.: #69480]	glucose-util	yes	88.704	no
[Ref.: #69480]	flagellated	no	96.722	no
[Ref.: #69480]	thermophile	no	98.938	yes
[Ref.: #69480]	glucose-ferment	no	91.966	no

Availability in culture collections External links

[Ref.: #11860]

Culture collection no.

DSM 44664, AS 4.0578, JCM 11373, BCRC 16960, CGMCC 4.0578, CIP 107743, NBRC 100042

[Ref.: #82512]

SI-ID 100722

Literature:

	Title	Authors	Journal	DOI	Year
Phylogeny	Description of Lentzea flaviverrucosa sp. nov. and transfer of the type strain of Saccharothrix aerocolonigenes subsp. staurosporea to Lentzea albida.	Xie Q, Wang Y, Huang Y, Wu Y, Ba F, Liu Z	Int J Syst Evol Microbiol	10.1099/00207713-52-5-1815	2002	*
	Discovery of unusual dimeric piperazyl cyclopeptides encoded by a Lentzea flaviverrucosa DSM 44664 biosynthetic supercluster.	Li C, Hu Y, Wu X, Stumpf SD, Qi Y, D'Alessandro JM, Nepal KK, Sarotti AM, Cao S, Blodgett JAV	Proc Natl Acad Sci U S A	10.1073/pnas.2117941119	2022	*

References

#11860

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 44664

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#39933 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#82512

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID100722.1 )

#119380 Collection of Institut Pasteur ; Curators of the CIP; CIP 107743
* These data were automatically processed and therefore are not curated

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Lentzea flaviverrucosa DSM 44664

Lentzea flaviverrucosa strain DSM 44664

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