Actinotalea caeni (KCTC 33604, JCM 30447, EBR-4-2)

Name: Actinotalea caeni (KCTC 33604, JCM 30447, EBR-4-2)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 133106

Type strain

Strain Designation EBR-4-2

Culture col. no. KCTC 33604 JCM 30447 EBR-4-2

NCBI tax ID(s) 1348467

Special Collections JCM KCTC Literature strains

History JCM <-- L. Jin; KRIBB, South Korea; EBR-4-2.

Links

Actinotalea caeni EBR-4-2 is an aerobe, Gram-positive, rod-shaped bacterium that forms circular colonies and was isolated from sludge of a biofilm reactor.

Gram-positive rod-shaped colony-forming aerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 287479

KCTC 33604,JCM 30447

@ref 20215

Last LPSN update 2026-05-29 08:38:01

Domain Bacteria

Phylum Actinomycetota

Class Actinomycetes

Order Micrococcales

Family Cellulomonadaceae

Genus Actinotalea

Species Actinotalea caeni

Full scientific name Actinotalea caeni Jin et al. 2017

Synonyms (1)

"Pseudactinotalea caeni"

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Motility	Confidence
25310	positive	rod-shaped
125439	positive			98.679

Colony morphology

@ref	Colony color	Colony shape	Medium used
25310	light yellow	circular	R2A

Culture and growth conditions

Culture medium

@ref	Name	Growth
25310	Reasoner's 2A agar (R2A)

Culture temp

@ref	Growth	Type	Temperature (°C)
25310	positive	growth	10-55
25310	positive	optimum	30
67770	positive	growth	28

Culture pH

@ref	Ability	Type	PH	PH range
25310	positive	optimum	7
25310	positive	growth	6.0-9.0	alkaliphile

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
25310	aerobe
125439	obligate aerobe	99.816

Spore formation

25310

Spore formationno

Halophily

@ref	Salt	Growth	Tested relation	Concentration
25310	NaCl		optimum	3.0-5.0 %
25310	NaCl	positive	growth	1.0-8.0 %(w/v)

Observation

67770

Observationquinones: MK-10(H₄)

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
25310		2-oxogluconate	-	assimilation
25310	16193 ChEBI	3-hydroxybenzoate	-	assimilation
25310	37054 ChEBI	3-hydroxybutyrate	-	assimilation
25310	17879 ChEBI	4-hydroxybenzoate	-	assimilation
25310	58143 ChEBI	5-dehydro-D-gluconate	-	assimilation
25310	30089 ChEBI	acetate	+	assimilation
25310	17128 ChEBI	adipate	-	assimilation
25310	17968 ChEBI	butyrate	-	assimilation
25310	16947 ChEBI	citrate	-	assimilation
25310	17634 ChEBI	D-glucose	+	assimilation
25310	16899 ChEBI	D-mannitol	+	assimilation
25310	16024 ChEBI	D-mannose	-	assimilation
25310	16988 ChEBI	D-ribose	-	assimilation
25310	17924 ChEBI	D-sorbitol	-	assimilation
25310	4853 ChEBI	esculin	+	hydrolysis
25310	5291 ChEBI	gelatin	+	hydrolysis
25310	24265 ChEBI	gluconate	-	assimilation
25310	17234 ChEBI	glucose	-	builds acid from
25310	28087 ChEBI	glycogen	+	assimilation
25310	27570 ChEBI	histidine	-	assimilation
25310	17240 ChEBI	itaconate	-	assimilation
25310	16977 ChEBI	L-alanine	-	assimilation
25310	30849 ChEBI	L-arabinose	+	assimilation
25310	18287 ChEBI	L-fucose	-	assimilation
25310	17203 ChEBI	L-proline	-	assimilation
25310	62345 ChEBI	L-rhamnose	-	assimilation
25310	17115 ChEBI	L-serine	-	assimilation
25310	24996 ChEBI	lactate	+	assimilation
25310	25115 ChEBI	malate	-	assimilation
25310	17306 ChEBI	maltose	+	assimilation
25310	28053 ChEBI	melibiose	+	assimilation
25310	17268 ChEBI	myo-inositol	-	assimilation
25310	506227 ChEBI	N-acetylglucosamine	+	assimilation
25310	17632 ChEBI	nitrate	-	reduction
25310	18401 ChEBI	phenylacetate	-	assimilation
25310	17272 ChEBI	propionate	-	assimilation
25310	17814 ChEBI	salicin	-	assimilation
25310	9300 ChEBI	suberic acid	-	assimilation
25310	17992 ChEBI	sucrose	+	assimilation
25310	31011 ChEBI	valerate	-	assimilation

Metabolite production

@ref	Chebi-ID	Metabolite	Production
25310	35581 ChEBI	indole

Enzymes

@ref	Value	Activity	Ec
25310	acid phosphatase	+	3.1.3.2
25310	alkaline phosphatase	-	3.1.3.1
25310	alpha-chymotrypsin	+	3.4.21.1
25310	alpha-fucosidase	-	3.2.1.51
25310	alpha-galactosidase	-	3.2.1.22
25310	alpha-glucosidase	+	3.2.1.20
25310	alpha-mannosidase	+	3.2.1.24
25310	arginine dihydrolase	-	3.5.3.6
25310	beta-galactosidase	+	3.2.1.23
25310	beta-glucosidase	-	3.2.1.21
25310	beta-glucuronidase	-	3.2.1.31
25310	catalase	+	1.11.1.6
25310	cystine arylamidase	+	3.4.11.3
25310	cytochrome oxidase	+	1.9.3.1
25310	esterase (C 4)	+
25310	esterase Lipase (C 8)	+
25310	leucine arylamidase	+	3.4.11.1
25310	lipase (C 14)	+
25310	N-acetyl-beta-glucosaminidase	+	3.2.1.52
25310	naphthol-AS-BI-phosphohydrolase	+
25310	trypsin	+	3.4.21.4
25310	urease	-	3.5.1.5
25310	valine arylamidase	+

Fatty acid profile

Metadata FA analysis

@ref

25310

Fatty acid	Percentage
C15:0 anteiso	32.8
C16:0	23.1
N alcohol C16:0	14.6
C15:1 anteiso A	9.4
C15:0 iso	5.3
C17:0 anteiso	4.2
C14:0	3.1
C16:0 iso	2
C17:0	1.8
C17:0 iso	1.6
C14:0 iso	1.5
C18:0	0.7

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Engineered	#Bioreactor	-
#Environmental	#Terrestrial	#Mud (Sludge)
#Environmental	#Biofilm	-

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
25310	sludge of a biofilm reactor	Daejeon, Republic of Korea	Republic of Korea	KOR	Asia
67770	Biofilm reactor	Daejeon	Republic of Korea	KOR	Asia

Taxonmaps

69479

Global distribution of 16S sequence KF056990 (>99% sequence identity) for Cellulomonadaceae from Microbeatlas

Aquatic Samples	398
Soil Samples	388
Animal Samples	641
Plant Samples	145
Total Samples	1572

Interaction and safety

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
67770	ASM973379v1 assembly for Actinotalea caeni JCM 30447	contig	GCA_009733795	1348467.3	2890385099	1348467	64.74

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
25310	Actinotalea caeni strain EBR-4-2 16S ribosomal RNA gene, partial sequence	KF056990	1463		1348467

GC content

@ref	GC-content (mol%)	Method
25310	74.8	high performance liquid chromatography (HPLC)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: ASM973379v1 assembly for Actinotalea caeni JCM 30447
NCBI: GCA_009733795

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	99.82	no
125439	motility	BacteriaNetⓘ	no	78.14	no
125439	gram_stain	BacteriaNetⓘ	positive	98.68	no
125439	spore_formation	BacteriaNetⓘ	no	74.91	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Actinotalea caeni strain JCM 30447
PATRIC: 1348467.3

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	86.76	no
125438	anaerobic	anaerobicⓘ	no	95.35	yes
125438	spore-forming	spore-formingⓘ	no	72.85	no
125438	aerobic	aerobicⓘ	yes	86.25	yes
125438	thermophilic	thermophileⓘ	no	95.38	yes
125438	flagellated	motile2+ⓘ	no	74.50	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Genetics	Physiological and Genomic Characterization of Actinotalea subterranea sp. nov. from Oil-Degrading Methanogenic Enrichment and Reclassification of the Family Actinotaleaceae.	Semenova EM, Grouzdev DS, Sokolova DS, Tourova TP, Poltaraus AB, Potekhina NV, Shishina PN, Bolshakova MA, Avtukh AN, Ianutsevich EA, Tereshina VM, Nazina TN.	Microorganisms	10.3390/microorganisms10020378	2022
Phylogeny	Actinotalea caeni sp. nov., isolated from a sludge sample of a biofilm reactor.	Jin L, Ko SR, Lee CS, Ahn CY, Lee JS, Lee KC, Oh HM, Lee HG	Int J Syst Evol Microbiol	10.1099/ijsem.0.001769	2017

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#25310	L. K. Jin, S. R.,Lee, C. S.,Ahn, C. Y.,Lee, J. S.,Lee, K. C.,Oh, H. M.,Lee, H. G.: Actinotalea caeni sp. nov., isolated from a sludge sample of a biofilm reactor. IJSEM 67: 1595 - 1599 2017 ( DOI 10.1099/ijsem.0.001769 , PubMed 28036250 )
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

Content

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Actinotalea caeni (KCTC 33604, JCM 30447, EBR-4-2)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Observation

Metabolite utilization

Metabolite production

Enzymes

Fatty acid profile

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Sequence information

Genome sequences

Genome browser: GCA_009733795

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

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