Name: Chloroflexus islandicus isl-2
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 133098

Type strain:

Species: Chloroflexus islandicus

Strain Designation: isl-2

Culture col. no.: DSM 29225, JCM 30533, VKM B-2978

Strain history: V. A. Gaisin; Centre Bioeng. RAS, Russia; isl-2.

NCBI tax ID(s): 1707952 (species)

SI-ID 399737

DSM 29225

Section

Chloroflexus islandicus isl-2 is an aerobe, photoheterotroph, thermophilic bacterium that forms filamentous colonies and builds filaments.

colony-forming
filament-shaped
Gram-negative
motile
pigmented
aerobe
photoheterotroph
thermophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Chloroflexota*
	Class	*Chloroflexia*
	Order	*Chloroflexales*
	Family	*Chloroflexaceae*
	Genus	*Chloroflexus*
	Species	**Chloroflexus islandicus**
	Full Scientific Name (LPSN)	Chloroflexus islandicus Gaisin et al. 2017

Information on morphological and physiological properties Morphology

[Ref.: #43175]	Gram stain	negative

[Ref.: #43175]	Cell length	4-7 µm

[Ref.: #43175]	Cell width	0.6 µm

[Ref.: #43175]	Cell shape	filament-shaped

[Ref.: #43175]	Motility	yes

[Ref.: #43175]	Flagellum arrangement	gliding

[Ref.: #43175]	Colony color	green
[Ref.: #43175]	Colony shape	filamentous

[Ref.: #43175]	Pigment production	yes
[Ref.: #43175]	Pigmentation color	green
[Ref.: #43175]	Pigment name	bacteriochlorophyll c
[Ref.: #43175]	Pigment production	yes
[Ref.: #43175]	Pigment name	bacteriochlorophyll a
[Ref.: #43175]	Pigment production	yes
[Ref.: #43175]	Pigment name	beta-carotene
[Ref.: #43175]	Pigment production	yes
[Ref.: #43175]	Pigment name	gamma-carotene

[Ref.: #43175]	Multicellular complex forming ability	yes
[Ref.: #43175]	Multicellular complex name	filaments
[Ref.: #43175]	Multicellular complex further description	unbranched multicellular filaments of varying lengths

Information on culture and growth conditions Culture and growth conditions

[Ref.: #24886]

Culture medium

MEDIUM FOR BROAD RANGE PHOTOTROPHIC BACTERIA (DSMZ Medium 1561)

[Ref.: #24886]

Culture medium growth

[Ref.: #24886]

Culture medium link

Medium recipe at MediaDive

[Ref.: #24886]

Culture medium composition

expand / minimize

[Ref.: #43175]

Culture medium growth

[Ref.: #43175]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #43175]	growth	46-59 °C
[Ref.: #43175]	optimum	55 °C
[Ref.: #67770]	growth	55 °C

[Ref.: #43175]	Temperature range	thermophilic
[Ref.: #67770]	Temperature range	thermophilic

	pH	Kind of pH		pH
[Ref.: #43175]			optimum	7.5-7.7

Information on physiology and metabolism Physiology and metabolism

[Ref.: #43175]	Oxygen tolerance	aerobe
[Ref.: #43175]	Oxygen tolerance	anaerobe

[Ref.: #43175]	Nutrition type	photoheterotroph
[Ref.: #43175]	Nutrition type	chemoheterotroph

[Ref.: #43175]

Ability of spore formation

[Ref.: #43175]	Observation	chlorosomes are present
[Ref.: #43175]	Observation	folic acid required as growth factor
[Ref.: #67770]	Observation	quinones: MK-10

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #43175]	acetate ChEBI	+	aerobic growth
[Ref.: #43175]	acetate ChEBI	+	anaerobic growth with light
[Ref.: #43175]	ammonium chloride ChEBI	+	nitrogen source
[Ref.: #43175]	aspartate ChEBI	+	anaerobic growth with light
[Ref.: #43175]	bromosuccinate ChEBI	+	anaerobic growth with light
[Ref.: #43175]	casamino acids	+	aerobic growth
[Ref.: #43175]	casamino acids	+	anaerobic growth with light
[Ref.: #43175]	casein hydrolysate	+	aerobic growth
[Ref.: #43175]	casein hydrolysate	+	anaerobic growth with light
[Ref.: #43175]	ethanol ChEBI	+	anaerobic growth with light
[Ref.: #43175]	fructose ChEBI	+	aerobic growth
[Ref.: #43175]	fructose ChEBI	+	anaerobic growth with light
[Ref.: #43175]	glucose ChEBI	+	aerobic growth
[Ref.: #43175]	glucose ChEBI	+	anaerobic growth with light
[Ref.: #43175]	glutamate ChEBI	+	anaerobic growth with light
[Ref.: #43175]	glycerol ChEBI	+	aerobic growth
[Ref.: #43175]	glycerol ChEBI	+	anaerobic growth with light
[Ref.: #43175]	glycylglycine ChEBI	+	anaerobic growth with light
[Ref.: #43175]	mannitol ChEBI	+	anaerobic growth with light
[Ref.: #43175]	methanol ChEBI	+	anaerobic growth with light
[Ref.: #43175]	pyruvate ChEBI	+	anaerobic growth with light
[Ref.: #43175]	soyton	+	aerobic growth
[Ref.: #43175]	soyton	+	anaerobic growth with light
[Ref.: #43175]	sucrose ChEBI	+	aerobic growth
[Ref.: #43175]	sucrose ChEBI	+	anaerobic growth with light
[Ref.: #43175]	yeast extract	+	aerobic growth
[Ref.: #43175]	yeast extract	+	anaerobic growth with light
	Only first 10 entries are displayed. Click here to see all.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #24886]	Sample type/isolated from	water-sand suspension from a geyser basin
[Ref.: #24886]	Geographic location (country and/or sea, region)	geyser Strokkur
[Ref.: #24886]	Country	Iceland
[Ref.: #24886]	Country ISO 3 Code	ISL
[Ref.: #24886]	Continent	Europe

[Ref.: #43175]	Sample type/isolated from	water-sand suspension
[Ref.: #43175]	Geographic location (country and/or sea, region)	Strokkur Geyser basin
[Ref.: #43175]	Country	Iceland
[Ref.: #43175]	Country ISO 3 Code	ISL
[Ref.: #43175]	Continent	Europe
[Ref.: #43175]	Enrichment culture	enrichment medium
[Ref.: #43175]	Enrichment culture composition	KH2PO4 0.5 g/l; NH4Cl 0.5 g/l; MgCl2x6H2O 0.3 g/l; KCl 0.5 g/l; NaCl 0.5 g/l; Na2S2O3 0.1 g/l; CaCl2x2H2O 0.05 g/l; Na2Sx9H2O 0.05 g/l; yeast extract 0.3 g/l; sodium acetate 0.5 g/l; sodium malate 0.5 g/l; HEPES 3.0 g/l; trace-element solution 1 ml/l; and Pfennig’s vitamin solution 1 ml/l, pH 8
[Ref.: #43175]	Enrichment culture temperature	54 ̊C
[Ref.: #43175]	Isolation/enrichment procedure	grown in the light

[Ref.: #67770]	Sample type/isolated from	Water-sand suspension sampled from the Strokkur Geyser basin (overflow near the channel)
[Ref.: #67770]	Country	Iceland
[Ref.: #67770]	Country ISO 3 Code	ISL
[Ref.: #67770]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	-
#Environmental	#Terrestrial	#Sandy
#Condition	#Thermophilic (>45°C)	-

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence KP939041 (>99% sequence identity) for Chloroflexus islandicus subclade from Microbeatlas

Aquatic Samples	206
Soil Samples	193
Animal Samples	119
Plant Samples	7
Total Samples	525

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #24886]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #43175]	Chloroflexus islandicus strain isl-2 16S ribosomal RNA gene, partial sequence	KP939041	1482	GenBank	1707952 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Chloroflexus islandicus isl-2	GCA_001650695	scaffold	GenBank	1707952 tax ID
[Ref.: #66792]	Chloroflexus islandicus isl-2	2775507287	complete	JGI IMG/M	1707952 tax ID	*

[Ref.: #43175]	GC-content	59.65 mol%	genome sequence analysis
[Ref.: #24886]	GC-content	59.65 mol%	sequence analysis

Availability in culture collections External links

[Ref.: #24886]

Culture collection no.

DSM 29225, JCM 30533, VKM B-2978

[Ref.: #91362]

SI-ID 399737

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Chloroflexus islandicus sp. nov., a thermophilic filamentous anoxygenic phototrophic bacterium from a geyser.	Gaisin VA, Kalashnikov AM, Grouzdev DS, Sukhacheva MV, Kuznetsov BB, Gorlenko VM	Int J Syst Evol Microbiol	10.1099/ijsem.0.001820	2017	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#24886

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 29225

#43175

Vasil A. Gaisin, Alexander M. Kalashnikov, Denis S. Grouzdev, Marina V. Sukhacheva, Boris B. Kuznetsov, Vladimir M. Gorlenko: Chloroflexus islandicus sp. nov., a thermophilic filamentous anoxygenic phototrophic bacterium from a geyser. IJSEM 67: 1381 - 1386 2017 ( DOI 10.1099/ijsem.0.001820 , PubMed 28126046 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#91362

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID399737.1 )

* These data were automatically processed and therefore are not curated

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