Name: Macrococcus canis KM 45013
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 133084

Type strain:

Species: Macrococcus canis

Strain Designation: KM 45013

Culture col. no.: DSM 101690, CCOS 969, CCUG 68920, CIP 111363

Strain history: CIP <- 2017, DSMZ <- Bern Univ., Bern, Switzerland: strain KM 45013

NCBI tax ID(s): 1855823 (species)

SI-ID 405974

DSM 101690, CCUG 68920, CIP 111363

Section

Macrococcus canis KM 45013 is a facultative anaerobe, psychrophilic, Gram-positive bacterium that forms circular colonies and was isolated from nose of Bernese mountain dog with mucopurulent rhinitis.

colony-forming
Gram-positive
rod-shaped
facultative anaerobe
psychrophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Staphylococcaceae*
	Genus	*Macrococcus*
	Species	**Macrococcus canis**
	Full Scientific Name (LPSN)	Macrococcus canis Gobeli Brawand et al. 2017

Synonym

"Macrococcoides canis"

Information on morphological and physiological properties Morphology

[Ref.: #25240]	Gram stain	positive
[Ref.: #117136]	Gram stain	positive

[Ref.: #25240]	Cell length	0.8 µm

[Ref.: #25240]	Cell shape	rod-shaped
[Ref.: #117136]	Cell shape	coccus-shaped

[Ref.: #25240]	Motility	no
[Ref.: #117136]	Motility	no

[Ref.: #25240]	Type of hemolysis	CAMP test
[Ref.: #25240]	Hemolysis ability	1
[Ref.: #25240]	Colony size	2 mm
[Ref.: #25240]	Colony color	greyish white
[Ref.: #25240]	Colony shape	circular
[Ref.: #25240]	Incubation period	1 day
[Ref.: #25240]	Cultivation medium used	TSA-SB agar

Information on culture and growth conditions Culture and growth conditions

[Ref.: #25240]

Culture medium

TSA-SB agar

[Ref.: #25240]

Culture medium growth

[Ref.: #24872]

Culture medium

TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)

[Ref.: #24872]

Culture medium growth

[Ref.: #24872]

Culture medium link

Medium recipe at MediaDive

[Ref.: #24872]

Culture medium composition

expand / minimize

[Ref.: #24872]

Culture medium

COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)

[Ref.: #24872]

Culture medium growth

[Ref.: #24872]

Culture medium link

Medium recipe at MediaDive

[Ref.: #24872]

Culture medium composition

expand / minimize

[Ref.: #117136]

Culture medium

CIP Medium 72

[Ref.: #117136]

Culture medium growth

[Ref.: #117136]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #24872]	growth	28 °C
[Ref.: #25240]	growth	18 °C
[Ref.: #25240]	growth	37 °C
[Ref.: #25240]	growth	43 °C
[Ref.: #63530]	growth	37 °C

[Ref.: #24872]	Temperature range	mesophilic
[Ref.: #25240]	Temperature range	psychrophilic
[Ref.: #25240]	Temperature range	mesophilic
[Ref.: #25240]	Temperature range	thermophilic
[Ref.: #63530]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #25240]	Oxygen tolerance	facultative anaerobe
[Ref.: #63530]	Oxygen tolerance	aerobe

Note that the displayed test results represent raw data and therefore may deviate!

Antibiotic susceptibility testing

Test 1

Test 2

Reference

[Ref.: #24872]
Mueller-Hinton Agar
37

aerob
yes
Inhibition zone diameter in mm
16-18
10
16-18
18-20
18
28-30
24-26
16
resistant0
44
40
34-36
34-26
26
22
32
22
30
30
10
16-18
26
20
14
28
22-24
40
10
30
36
30-32
38-40
resistant0
26-28
18
22

[Ref.: #24872]
Mueller-Hinton Agar
37

aerob
yes
Inhibition zone diameter in mm
16-18
10
16-18
18-20
18
28-30
24-26
16
resistant0
44
40
34-36
34-26
26
22
32
22
30
30
10
16-18
26
20
14
28
22-24
40
10
30
36
30-32
38-40
resistant0
26-28
18
22

Only first 15 entries are displayed. Click here to see all.

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #25240]		NaCl		growth	9	%
[Ref.: #25240]		NaCl		growth	12	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #25240]	(R)-amygdalin ChEBI	-	assimilation
[Ref.: #68375]	arabinose ChEBI	-	fermentation	* from API ID32STA
[Ref.: #68375]	arginine ChEBI	-	hydrolysis	* from API ID32STA
[Ref.: #68375]	cellobiose ChEBI	-	fermentation	* from API ID32STA
[Ref.: #25240]	cyclodextrin ChEBI	-	assimilation
[Ref.: #68375]	D-fructose ChEBI	+	fermentation	* from API ID32STA
[Ref.: #25240]	D-galactose ChEBI	-	builds acid from
[Ref.: #68375]	D-glucose ChEBI	-	fermentation	* from API ID32STA
[Ref.: #25240]	D-mannitol ChEBI	+	builds acid from
[Ref.: #68375]	D-mannitol ChEBI	-	fermentation	* from API ID32STA
[Ref.: #25240]	D-mannose ChEBI	+	builds acid from
[Ref.: #68375]	D-mannose ChEBI	-	fermentation	* from API ID32STA
[Ref.: #25240]	D-ribose ChEBI	-	builds acid from
[Ref.: #68375]	D-ribose ChEBI	-	fermentation	* from API ID32STA
[Ref.: #25240]	D-sorbitol ChEBI	-	builds acid from
[Ref.: #25240]	D-xylose ChEBI	-	builds acid from
[Ref.: #68375]	esculin ChEBI	-	hydrolysis	* from API ID32STA
[Ref.: #25240]	lactose ChEBI	-	builds acid from
[Ref.: #68375]	lactose ChEBI	-	fermentation	* from API ID32STA
[Ref.: #25240]	maltose ChEBI	+	builds acid from
[Ref.: #68375]	maltose ChEBI	-	fermentation	* from API ID32STA
[Ref.: #68375]	N-acetylglucosamine ChEBI	-	fermentation	* from API ID32STA
[Ref.: #68375]	nitrate ChEBI	+	reduction	* from API ID32STA
[Ref.: #68375]	ornithine ChEBI	-	degradation	* from API ID32STA
[Ref.: #25240]	raffinose ChEBI	-	builds acid from
[Ref.: #68375]	raffinose ChEBI	-	fermentation	* from API ID32STA
[Ref.: #25240]	salicin ChEBI	-	assimilation
[Ref.: #25240]	sucrose ChEBI	-	builds acid from
[Ref.: #68375]	sucrose ChEBI	-	fermentation	* from API ID32STA
[Ref.: #25240]	trehalose ChEBI	+	builds acid from
[Ref.: #68375]	trehalose ChEBI	-	fermentation	* from API ID32STA
[Ref.: #68375]	turanose ChEBI	-	fermentation	* from API ID32STA
[Ref.: #68375]	urea ChEBI	-	hydrolysis	* from API ID32STA
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Antibiotic sensitivity	Concentration		Antibiotic resistance
[Ref.: #25240]	bacitracin ChEBI				no
[Ref.: #25240]	novobiocin ChEBI				yes
[Ref.: #25240]	optochin ChEBI				yes
[Ref.: #25240]	polymyxin b ChEBI				no
[Ref.: #25240]	vibriostat ChEBI				yes
[Ref.: #68375]	novobiocin ChEBI	yes	1.8	µg		* from API ID32STA

	Metabolite production	Metabolite	Production
[Ref.: #68375]		acetoin ChEBI	yes	* from API ID32STA

	Physiological tests	Metabolite	Voges-Proskauer-test
[Ref.: #68375]		acetoin ChEBI	+	* from API ID32STA

	Enzyme	Enzyme activity	EC number
[Ref.: #25240]	alanine arylamidase	-	3.4.11.2
[Ref.: #68375]	alkaline phosphatase	-	3.1.3.1	* from API ID32STA
[Ref.: #25240]	alpha-galactosidase	-	3.2.1.22
[Ref.: #25240]	alpha-glucosidase	+	3.2.1.20
[Ref.: #25240]	alpha-mannosidase	-	3.2.1.24
[Ref.: #25240]	arginine dihydrolase	+	3.5.3.6
[Ref.: #25240]	arginine dihydrolase	-	3.5.3.6
[Ref.: #68375]	arginine dihydrolase	-	3.5.3.6	* from API ID32STA
[Ref.: #25240]	beta-galactopyranosidase	-
[Ref.: #25240]	beta-galactosidase	-	3.2.1.23
[Ref.: #68375]	beta-galactosidase	-	3.2.1.23	* from API ID32STA
[Ref.: #68375]	beta-glucosidase	-	3.2.1.21	* from API ID32STA
[Ref.: #25240]	beta-glucuronidase	-	3.2.1.31
[Ref.: #68375]	beta-glucuronidase	-	3.2.1.31	* from API ID32STA
[Ref.: #25240]	catalase	+	1.11.1.6
[Ref.: #25240]	cytochrome oxidase	+	1.9.3.1
[Ref.: #25240]	DNase	+
[Ref.: #68375]	L-arginine arylamidase	-		* from API ID32STA
[Ref.: #25240]	L-aspartate arylamidase	-	3.4.11.21
[Ref.: #25240]	leucine arylamidase	+	3.4.11.1
[Ref.: #68375]	ornithine decarboxylase	-	4.1.1.17	* from API ID32STA
[Ref.: #25240]	phosphatase	-
[Ref.: #25240]	phosphatidylinositol phospholipase C	-	3.1.4.11
[Ref.: #25240]	proline-arylamidase	-	3.4.11.5
[Ref.: #25240]	pyrrolidonyl arylamidase	+	3.4.19.3
[Ref.: #68375]	pyrrolidonyl arylamidase	-	3.4.19.3	* from API ID32STA
[Ref.: #25240]	tripeptide aminopeptidase	-	3.4.11.4
[Ref.: #25240]	tyrosine arylamidase	+
[Ref.: #25240]	urease	-	3.5.1.5
[Ref.: #68375]	urease	-	3.5.1.5	* from API ID32STA
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #25240]

Fatty acid	Percentage
C14:0	29.5
C18:3ω6c (6,9,12)	22.3
C16:0 n alcohol	20.7
C16:1ω11c	18.4
C16:0	5
C18:0	1.5
C14:0 iso	1.2
C12:0 iso	0.9
C20:0	0.7

API® ID32STA:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Urease/urea hydrolysisURE	Arginine dihydrolaseADH (Arg)	Ornithine decarboxylaseODC	Esculin hydrolysisESC	Fermentation of D-glucoseGLU	Fermentation of D-fructoseFRU	Fermentation of D-mannoseMNE	Fermentation of maltoseMAL	Fermentation of lactoseLAC	Fermentation of trehaloseTRE	Fermentation of D-mannitolMAN	Fermentation of raffinoseRAF	Fermentation of D-riboseRIB	Fermentation of cellobioseCEL	Reduction of nitrateNIT	Acetoin production (Voges Proskauer test)VP	beta-Galactosidasebeta GAL	L-arginine arylamidaseArgA	Alkaline phosphatasePAL	Pyrrolidonyl arylamidasePyrA	Novobiocin resistanceNOVO	Fermentation of sucroseSAC	Fermentation of N-acetyl-glucosamineNAG	Fermentation of turanoseTUR	Fermentation of arabinoseARA	beta-Glucuronidasebeta GUR
[Ref.: #24872]	6235	-	-	-	-	-	+	-	-	-	-	-	-	-	-	+	+	-	-	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #24872]	Sample type/isolated from	nose of Bernese mountain dog with mucopurulent rhinitis
[Ref.: #24872]	Country	Switzerland
[Ref.: #24872]	Country ISO 3 Code	CHE
[Ref.: #24872]	Continent	Europe

[Ref.: #63530]	Sample type/isolated from	Sinusitis,Bernese Mountain dog
[Ref.: #63530]	Sampling date	2013-04-30
[Ref.: #63530]	Geographic location (country and/or sea, region)	Bern
[Ref.: #63530]	Country	Switzerland
[Ref.: #63530]	Country ISO 3 Code	CHE
[Ref.: #63530]	Continent	Europe

[Ref.: #117136]	Sample type/isolated from	Animal, Bernese mountain dog, mucopurulent rhinitis
[Ref.: #117136]	Geographic location (country and/or sea, region)	Bern
[Ref.: #117136]	Country	Switzerland
[Ref.: #117136]	Country ISO 3 Code	CHE
[Ref.: #117136]	Continent	Europe

[Ref.: #25240]	Enrichment culture	trypticase soy agar
[Ref.: #25240]	Enrichment culture composition	containing 5 % sheep blood (TSA-SB; Becton Dickinson)
[Ref.: #25240]	Enrichment culture duration	1 day
[Ref.: #25240]	Enrichment culture temperature	37 ̊C
* marker position based on {}

Isolation sources categories

#Infection	#Inflammation	-
#Host	#Mammals	#Canidae (Dog)
#Host Body-Site	#Organ	#Nose

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence KU933342 (>99% sequence identity) for Macrococcus from Microbeatlas

Aquatic Samples	930
Soil Samples	1069
Animal Samples	8379
Plant Samples	372
Total Samples	10750

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #117136]

Biosafety level

Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #24872]	Macrococcus canis strain KM 45013 16S ribosomal RNA gene, partial sequence	KU933342	1547	ENA	1855823 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Macrococcus canis KM 45013	GCA_002119805	complete	GenBank	1855823 tax ID	*

[Ref.: #24872]

GC-content

36.9 mol%

high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Macrococcus canis KM 45013 NCBI: GCA_002119805.1
[Ref.: #69480]	flagellated	no	92.07	yes
[Ref.: #69480]	gram-positive	yes	93.802	yes
[Ref.: #69480]	anaerobic	no	98.704	no
[Ref.: #69480]	halophile	yes	87.157	yes
[Ref.: #69480]	spore-forming	no	78.565	no
[Ref.: #69480]	glucose-util	yes	88.419	no
[Ref.: #69480]	aerobic	yes	88.091	no
[Ref.: #69480]	thermophile	no	99.855	no
[Ref.: #69480]	glucose-ferment	no	73.346	no
[Ref.: #69480]	motile	no	88.685	yes

Availability in culture collections External links

[Ref.: #24872]

Culture collection no.

DSM 101690, CCOS 969, CCUG 68920, CIP 111363

[Ref.: #91349]

SI-ID 405974

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Macrococcus armenti sp. nov., a novel bacterium isolated from the skin and nasal cavities of healthy pigs and calves.	Keller JE, Schwendener S, Overesch G, Perreten V	Int J Syst Evol Microbiol	10.1099/ijsem.0.005245	2022	*
Phylogeny	Macrococcus canis sp. nov., a skin bacterium associated with infections in dogs.	Gobeli Brawand S, Cotting K, Gomez-Sanz E, Collaud A, Thomann A, Brodard I, Rodriguez-Campos S, Strauss C, Perreten V	Int J Syst Evol Microbiol	10.1099/ijsem.0.001673	2017	*
Genetics	Complete Genome Sequence of the Type Strain of Macrococcus canis.	Gobeli Brawand S, Rychener L, Schwendener S, Pantucek R, Perreten V	Genome Announc	10.1128/genomeA.01507-17	2018	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#24872

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 101690

#25240

S. C. Gobeli Brawand, Kerstin,Gómez-Sanz, Elena,Collaud, Alexandra,Thomann, Andreas,Brodard, Isabelle,Rodriguez-Campos, Sabrina,Strauss, Christian,Perreten, Vincent: Macrococcus canis sp. nov., a skin bacterium associated with infections in dogs. IJSEM 67: 621 - 626 2017 ( DOI 10.1099/ijsem.0.001673 , PubMed 27902286 )

#63530 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 68920

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68375

Automatically annotated from API ID32STA .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#91349

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID405974.1 )

#117136 Collection of Institut Pasteur ; Curators of the CIP; CIP 111363
* These data were automatically processed and therefore are not curated

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