Name: Pseudactinotalea terrae 5GHs33-03
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 133083

Type strain:

Species: Pseudactinotalea terrae

Strain Designation: 5GHs33-03

Culture col. no.: DSM 27157, KACC 16542, NBRC 111006

Strain history: <- S.-W. Kwon, Korean Agricultural Collection, KACC; 5GHs33-03

NCBI tax ID(s): 1743262 (species)

SI-ID 401644

DSM 27157

Section

Pseudactinotalea terrae 5GHs33-03 is an aerobe, Gram-negative, rod-shaped bacterium that forms circular colonies and was isolated from greenhouse soil.

colony-forming
Gram-negative
rod-shaped
aerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Micrococcales*
	Family	*Cellulomonadaceae*
	Genus	*Pseudactinotalea*
	Species	**Pseudactinotalea terrae**
	Full Scientific Name (LPSN)	Pseudactinotalea terrae Cho et al. 2017

Information on morphological and physiological properties Morphology

[Ref.: #25230]	Gram stain	negative
[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #25230]	Cell length	1.2-2.2 µm

[Ref.: #25230]	Cell width	0.8-1.0 µm

[Ref.: #25230]	Cell shape	rod-shaped

[Ref.: #25230]	Colony size	1 mm
[Ref.: #25230]	Colony color	yellow
[Ref.: #25230]	Colony shape	circular
[Ref.: #25230]	Incubation period	4 days
[Ref.: #25230]	Cultivation medium used	R2A

Information on culture and growth conditions Culture and growth conditions

[Ref.: #25230]

Culture medium

Reasoner's 2A agar (R2A)

[Ref.: #25230]

Culture medium growth

[Ref.: #24871]

Culture medium

R2A MEDIUM (DSMZ Medium 830)

[Ref.: #24871]

Culture medium growth

[Ref.: #24871]

Culture medium link

Medium recipe at MediaDive

[Ref.: #24871]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #24871]	growth	28 °C
[Ref.: #25230]	growth	4-37 °C
[Ref.: #25230]	optimum	28-30 °C

[Ref.: #24871]	Temperature range	mesophilic
[Ref.: #25230]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #25230]			optimum	7
[Ref.: #25230]			growth	6.0-10.0

Information on physiology and metabolism Physiology and metabolism

[Ref.: #25230]

Oxygen tolerance

aerobe

[Ref.: #69480]

Ability of spore formation

no Confidence in %99.959

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #25230]		NaCl		growth	0.0-7.0	%(w/v)
[Ref.: #25230]		NaCl		optimum	0	%(w/v)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #25230]	3-hydroxybenzoate ChEBI	-	assimilation
[Ref.: #25230]	3-hydroxybutyrate ChEBI	-	assimilation
[Ref.: #25230]	4-hydroxybenzoate ChEBI	-	assimilation
[Ref.: #25230]	adipate ChEBI	-	assimilation
[Ref.: #25230]	casein	-	hydrolysis
[Ref.: #25230]	cellulose ChEBI	-	hydrolysis
[Ref.: #25230]	chitin ChEBI	-	hydrolysis
[Ref.: #25230]	D-glucose ChEBI	+	assimilation
[Ref.: #25230]	D-mannitol ChEBI	+	assimilation
[Ref.: #25230]	D-mannose ChEBI	-	assimilation
[Ref.: #25230]	D-ribose ChEBI	+	assimilation
[Ref.: #25230]	D-sorbitol ChEBI	-	assimilation
[Ref.: #25230]	decanoate ChEBI	-	assimilation
[Ref.: #25230]	dna ChEBI	-	hydrolysis
[Ref.: #25230]	esculin ChEBI	+	hydrolysis
[Ref.: #25230]	gelatin ChEBI	+	hydrolysis
[Ref.: #25230]	glucose ChEBI	-	fermentation
[Ref.: #25230]	glycogen ChEBI	-	assimilation
[Ref.: #25230]	hypoxanthine ChEBI	-	hydrolysis
[Ref.: #25230]	itaconate ChEBI	-	assimilation
[Ref.: #25230]	L-alanine ChEBI	-	assimilation
[Ref.: #25230]	L-arabinose ChEBI	-	assimilation
[Ref.: #25230]	L-fucose ChEBI	-	assimilation
[Ref.: #25230]	L-histidine ChEBI	-	assimilation
[Ref.: #25230]	L-proline ChEBI	-	assimilation
[Ref.: #25230]	L-rhamnose ChEBI	+	assimilation
[Ref.: #25230]	L-serine ChEBI	-	assimilation
[Ref.: #25230]	lactate ChEBI	-	assimilation
[Ref.: #25230]	malate ChEBI	-	assimilation
[Ref.: #25230]	maltose ChEBI	+	assimilation
[Ref.: #25230]	melibiose ChEBI	+	assimilation
[Ref.: #25230]	myo-inositol ChEBI	-	assimilation
[Ref.: #25230]	N-acetylglucosamine ChEBI	+	assimilation
[Ref.: #25230]	nitrate ChEBI	+	reduction
[Ref.: #25230]	phenylacetate ChEBI	-	assimilation
[Ref.: #25230]	potassium 2-dehydro-D-gluconate	-	assimilation
[Ref.: #25230]	potassium 5-dehydro-D-gluconate	-	assimilation
[Ref.: #25230]	potassium gluconate ChEBI	-	assimilation
[Ref.: #25230]	propionate ChEBI	-	assimilation
[Ref.: #25230]	salicin ChEBI	-	assimilation
[Ref.: #25230]	sodium acetate ChEBI	-	assimilation
[Ref.: #25230]	sodium citrate ChEBI	-	assimilation
[Ref.: #25230]	sodium malonate ChEBI	-	assimilation
[Ref.: #25230]	starch ChEBI	-	hydrolysis
[Ref.: #25230]	suberic acid ChEBI	-	assimilation
[Ref.: #25230]	sucrose ChEBI	+	assimilation
[Ref.: #25230]	tween 80 ChEBI	-	hydrolysis
[Ref.: #25230]	tyrosine ChEBI	-	hydrolysis
[Ref.: #25230]	valerate ChEBI	-	assimilation
[Ref.: #25230]	xanthine ChEBI	-	hydrolysis
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #25230]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #25230]	acid phosphatase	-	3.1.3.2
[Ref.: #25230]	alkaline phosphatase	-	3.1.3.1
[Ref.: #25230]	alpha-chymotrypsin	-	3.4.21.1
[Ref.: #25230]	alpha-fucosidase	-	3.2.1.51
[Ref.: #25230]	alpha-galactosidase	-	3.2.1.22
[Ref.: #25230]	alpha-glucosidase	+	3.2.1.20
[Ref.: #25230]	alpha-mannosidase	+	3.2.1.24
[Ref.: #25230]	arginine dihydrolase	-	3.5.3.6
[Ref.: #25230]	beta-galactosidase	+	3.2.1.23
[Ref.: #25230]	beta-glucosidase	+	3.2.1.21
[Ref.: #25230]	beta-glucuronidase	-	3.2.1.31
[Ref.: #25230]	cystine arylamidase	-	3.4.11.3
[Ref.: #25230]	cytochrome oxidase	+	1.9.3.1
[Ref.: #25230]	esterase (C 4)	+
[Ref.: #25230]	esterase Lipase (C 8)	+
[Ref.: #25230]	leucine arylamidase	+	3.4.11.1
[Ref.: #25230]	lipase (C 14)	-
[Ref.: #25230]	N-acetyl-beta-glucosaminidase	+	3.2.1.52
[Ref.: #25230]	naphthol-AS-BI-phosphohydrolase	+
[Ref.: #25230]	trypsin	-	3.4.21.4
[Ref.: #25230]	urease	-	3.5.1.5
[Ref.: #25230]	valine arylamidase	+
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #25230]

Fatty acid	Percentage
C15:0 anteiso	70.1
C14:0 iso	15.2
C15:0 iso	6.8
C14:0	1.7
C13:0 iso	1.7

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #24871]	Sample type/isolated from	greenhouse soil
[Ref.: #24871]	Geographic location (country and/or sea, region)	Gyeonggi province, Yongin region
[Ref.: #24871]	Country	Republic of Korea
[Ref.: #24871]	Country ISO 3 Code	KOR
[Ref.: #24871]	Continent	Asia

[Ref.: #25230]	Enrichment culture	R2A medium
[Ref.: #25230]	Isolation/enrichment procedure	dilution method
* marker position based on {}

Isolation sources categories

#Engineered	#Agriculture	#Greenhouse
#Environmental	#Terrestrial	#Soil

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence KR869780 (>99% sequence identity) for Pseudactinotalea terrae from Microbeatlas

Aquatic Samples	25
Soil Samples	100
Animal Samples	128
Plant Samples	46
Total Samples	299

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #24871]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #24871]	Pseudactinotalea terrae strain 5GHs33-3 16S ribosomal RNA gene, partial sequence	KR869780	1462	ENA	1743262 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Pseudactinotalea terrae KACC 16542	GCA_009733845	contig	GenBank	1743262 tax ID	*
[Ref.: #66792]	Pseudactinotalea terrae strain KACC 16542	1743262.3	wgs	PATRIC	1743262 tax ID	*
[Ref.: #66792]	Pseudactinotalea terrae KACC 16542	2890205655	draft	JGI IMG/M	1743262 tax ID	*

[Ref.: #25230]	GC-content	69 mol%	Thermal denaturation, fluorometry
[Ref.: #24871]	GC-content	69.0 mol%	fluorimetric

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Pseudactinotalea terrae strain KACC 16542 PATRIC: 1743262.3
[Ref.: #69481]	spore-forming	no	65	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Pseudactinotalea terrae strain KACC 16542 PATRIC: 1743262.3
[Ref.: #69480]	motile	no	51.22	no
[Ref.: #69480]	gram-positive	yes	72.901	yes
[Ref.: #69480]	anaerobic	no	99.309	no
[Ref.: #69480]	aerobic	yes	89.224	yes
[Ref.: #69480]	halophile	no	93.703	no
[Ref.: #69480]	spore-forming	no	89.623	no
[Ref.: #69480]	thermophile	no	96.469	yes
[Ref.: #69480]	glucose-util	yes	86.516	yes
[Ref.: #69480]	flagellated	no	96.28	no
[Ref.: #69480]	glucose-ferment	no	84.027	yes

Availability in culture collections External links

[Ref.: #24871]

Culture collection no.

DSM 27157, KACC 16542, NBRC 111006

[Ref.: #91348]

SI-ID 401644

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Pseudactinotalea terrae gen. nov., sp. nov., isolated from greenhouse soil, and reclassification of Actinotalea suaedae as Pseudactinotalea suaedae comb. nov.	Cho H, Hamada M, Ahn JH, Weon HY, Joa JH, Suzuki KI, Kwon SW, Kim SJ	Int J Syst Evol Microbiol	10.1099/ijsem.0.001701	2017	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#24871

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 27157

#25230

H. H. Cho, M.,Ahn, J. H.,Weon, H. Y.,Joa, J. H.,Suzuki, K. I.,Kwon, S. W.,Kim, S. J.: Pseudactinotalea terrae gen. nov., sp. nov., isolated from greenhouse soil, and reclassification of Actinotalea suaedae as Pseudactinotalea suaedae comb. nov. IJSEM 67: 704 - 709 2017 ( DOI 10.1099/ijsem.0.001701 , PubMed 27902298 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#91348

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID401644.1 )

* These data were automatically processed and therefore are not curated

Change proposal

You found an error in BacDive? Please tell us about it!

Note that changes will be reviewed and judged. If your changes are legitimate, changes will occur within the next BacDive update. Only proposed changes supported by the according reference will be reviewed. The BacDive team reserves the right to reject proposed changes.

Search for species Pseudactinotalea terrae in external resources:
SILVA
BRENDA
PANGAEA
StrainInfo
GBIF

Pseudactinotalea terrae strain KACC 16542

Pseudactinotalea terrae strain KACC 16542

Change proposal

Change proposal