Name: Desulfonatronospira sulfatiphila ASO3-2
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 133075

Type strain:

Species: Desulfonatronospira sulfatiphila

Strain Designation: ASO3-2

Culture col. no.: DSM 100427, UNIQEM U993

Strain history: <- D. Sorokin, Dept. Biotechnol., Delft Univ. Technol., Netherlands; ASO3-2 <- D. Sorokin {2014}

NCBI tax ID(s): 1640152 (species)

SI-ID 403960

DSM 100427

Section

Desulfonatronospira sulfatiphila ASO3-2 is an obligate anaerobe, thermophilic, Gram-negative bacterium that was isolated from sediment of a hypersaline soda lake.

Gram-negative
rod-shaped
obligate anaerobe
thermophilic
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Deltaproteobacteria*
	Order	*Desulfovibrionales*
	Family	*Desulfohalobiaceae*
	Genus	*Desulfonatronospira*
	Species	**Desulfonatronospira sulfatiphila**
	Full Scientific Name (LPSN)	Desulfonatronospira sulfatiphila Sorokin and Chernyh 2017

Information on morphological and physiological properties Morphology

[Ref.: #25180]	Gram stain	negative

[Ref.: #25180]	Cell length	1.5-3.0 µm

[Ref.: #25180]	Cell width	0.7-0.8 µm

[Ref.: #25180]	Cell shape	rod-shaped

[Ref.: #25180]	Motility	no

Information on culture and growth conditions Culture and growth conditions

[Ref.: #25180]

Culture medium

mineral sodium carbonate-based medium

[Ref.: #25180]

Culture medium growth

[Ref.: #25180]

Culture medium composition

expand / minimize

[Ref.: #24863]

Culture medium

DESULFONATRONOSPIRA MEDIUM (DSMZ Medium 1101)

[Ref.: #24863]

Culture medium growth

[Ref.: #24863]

Culture medium link

Medium recipe at MediaDive

[Ref.: #24863]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #24863]	growth	30 °C
[Ref.: #25180]	maximum	40 °C
[Ref.: #25180]	growth	33.0-35.0 °C

[Ref.: #24863]	Temperature range	mesophilic
[Ref.: #25180]	Temperature range	thermophilic
[Ref.: #25180]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #25180]			optimum	9.7-10.0
[Ref.: #25180]			growth	9.0-10.3

Information on physiology and metabolism Physiology and metabolism

[Ref.: #25180]

Oxygen tolerance

obligate anaerobe

[Ref.: #25180]

Ability of spore formation

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #25180]		Na+		growth	1.0-4.0	M
[Ref.: #25180]		Na+		optimum	2	M

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #25180]	butanol ChEBI	+	energy source
[Ref.: #25180]	ethanol ChEBI	+	energy source
[Ref.: #25180]	formate ChEBI	+	energy source
[Ref.: #25180]	lactate ChEBI	+	energy source
[Ref.: #25180]	pyruvate ChEBI	+	energy source
[Ref.: #25180]	sulfate ChEBI	+	electron acceptor
[Ref.: #25180]	sulfite ChEBI	+	electron acceptor

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #24863]	Sample type/isolated from	sediment of a hypersaline soda lake
[Ref.: #24863]	Geographic location (country and/or sea, region)	Kulunda Steppe, Altai, lake Bitter-1
[Ref.: #24863]	Country	Russia
[Ref.: #24863]	Country ISO 3 Code	RUS
[Ref.: #24863]	Continent	Europe

[Ref.: #25180]	Sample type/isolated from	sediments of hypersaline soda lake Bitter-1
[Ref.: #25180]	Enrichment culture	pure culture
[Ref.: #25180]	Enrichment culture composition	using formate as electron donor and sulfate as electron acceptor
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	#Lake (large)
#Environmental	#Aquatic	#Sediment
#Condition	#Alkaline	-
#Condition	#Saline	-

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence KP223255 (>99% sequence identity) for Desulfonatronospira sulfatiphila from Microbeatlas

Aquatic Samples	4
Soil Samples
Animal Samples
Plant Samples
Total Samples	4

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #24863]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #24863]	Desulfonatronospira sulfatiphila strain ASO3-2 16S ribosomal RNA gene, partial sequence	KP223255	1590	ENA	1640152 tax ID

[Ref.: #24863]

GC-content

51.1 mol%

thermal denaturation, midpoint method (Tm)

Availability in culture collections External links

[Ref.: #24863]

Culture collection no.

DSM 100427, UNIQEM U993

[Ref.: #91340]

SI-ID 403960

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Desulfonatronospira sulfatiphila sp. nov., and Desulfitispora elongata sp. nov., two novel haloalkaliphilic sulfidogenic bacteria from soda lakes.	Sorokin DY, Chernyh NA	Int J Syst Evol Microbiol	10.1099/ijsem.0.001640	2017	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#24863

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 100427

#25180

D. Y. C. Sorokin, N. A.: Desulfonatronospira sulfatiphila sp. nov., and Desulfitispora elongata sp. nov., two novel haloalkaliphilic sulfidogenic bacteria from soda lakes. IJSEM 67: 396 - 401 2017 ( DOI 10.1099/ijsem.0.001640 , PubMed 27902279 )

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#91340

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID403960.1 )

* These data were automatically processed and therefore are not curated

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