Name: Cohnella lubricantis KSS-154-50
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 133071

Type strain:

Species: Cohnella lubricantis

Strain Designation: KSS-154-50

Culture col. no.: DSM 103658, CCM 8707, LMG 29763, CIP 111180

Strain history: CIP <- 2016, S. Glaeser, Giessen Univ., Giessen, Germany: strain KSS-154-50

NCBI tax ID(s): 2163172 (species)

SI-ID 399652

DSM 103658

Section

Cohnella lubricantis KSS-154-50 is an aerobe, spore-forming, mesophilic bacterium that forms circular colonies and was isolated from metalworking fluid, coolant lubricant.

colony-forming
Gram-positive
rod-shaped
spore-forming
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Paenibacillaceae*
	Genus	*Cohnella*
	Species	**Cohnella lubricantis**
	Full Scientific Name (LPSN)	Cohnella lubricantis Kämpfer et al. 2017

Information on morphological and physiological properties Morphology

[Ref.: #25177]	Gram stain	positive
[Ref.: #120656]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #25177]	Cell length	2.0-3.0 µm

[Ref.: #25177]	Cell width	0.8-1.0 µm

[Ref.: #25177]	Cell shape	rod-shaped
[Ref.: #120656]	Cell shape	rod-shaped

[Ref.: #25177]	Motility	no
[Ref.: #120656]	Motility	no
[Ref.: #69480]	Motility	yes Confidence in %97.926

[Ref.: #25177]	Type of hemolysis	gamma
[Ref.: #25177]	Colony color	beige
[Ref.: #25177]	Colony shape	circular
[Ref.: #25177]	Cultivation medium used	R2A agar

Information on culture and growth conditions Culture and growth conditions

[Ref.: #25177]

Culture medium

tryptic soy broth (TSB)

[Ref.: #25177]

Culture medium growth

[Ref.: #25177]

Culture medium

nutrient agar (NA)

[Ref.: #25177]

Culture medium growth

[Ref.: #25177]

Culture medium

tryptic soy agar (TSA)

[Ref.: #25177]

Culture medium growth

[Ref.: #25177]

Culture medium

Columbia agar

[Ref.: #25177]

Culture medium growth

[Ref.: #25177]

Culture medium composition

expand / minimize

[Ref.: #25177]

Culture medium

3.3xPYE agar

[Ref.: #25177]

Culture medium growth

[Ref.: #25177]

Culture medium

Reasoner's 2A agar (R2A)

[Ref.: #25177]

Culture medium growth

[Ref.: #24859]

Culture medium

NUTRIENT AGAR (DSMZ Medium 1)

[Ref.: #24859]

Culture medium growth

[Ref.: #24859]

Culture medium link

Medium recipe at MediaDive

[Ref.: #24859]

Culture medium composition

expand / minimize

[Ref.: #120656]

Culture medium

CIP Medium 72

[Ref.: #120656]

Culture medium growth

[Ref.: #120656]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #24859]		growth	30 °C
[Ref.: #25177]		optimum	28 °C
[Ref.: #25177]		growth	20.0-45.0 °C
[Ref.: #25177]	no	growth	15 °C
[Ref.: #25177]	no	growth	50 °C

[Ref.: #24859]	Temperature range	mesophilic
[Ref.: #25177]	Temperature range	mesophilic
[Ref.: #25177]	Temperature range	psychrophilic
[Ref.: #25177]	Temperature range	thermophilic

	pH	Kind of pH		pH
[Ref.: #25177]			optimum	6.5

Information on physiology and metabolism Physiology and metabolism

[Ref.: #25177]

Oxygen tolerance

aerobe

[Ref.: #25177]	Spore description	oval
[Ref.: #25177]	Type of spore	endospore
[Ref.: #25177]	Ability of spore formation	yes
[Ref.: #69481]	Ability of spore formation	yes Confidence in %100
[Ref.: #69480]	Ability of spore formation	yes Confidence in %100

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #25177]		NaCl	no	growth	3	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #25177]	2-oxoglutarate ChEBI	-	assimilation
[Ref.: #25177]	acetate ChEBI	-	assimilation
[Ref.: #25177]	adipate ChEBI	-	assimilation
[Ref.: #25177]	alanine ChEBI	-	assimilation
[Ref.: #25177]	arbutin ChEBI	-	assimilation
[Ref.: #25177]	azelaate ChEBI	-	assimilation
[Ref.: #25177]	casein	-	hydrolysis
[Ref.: #25177]	cellobiose ChEBI	-	builds acid from
[Ref.: #25177]	cellobiose ChEBI	-	assimilation
[Ref.: #25177]	cis-aconitate ChEBI	-	assimilation
[Ref.: #25177]	citrate ChEBI	-	carbon source
[Ref.: #25177]	citrate ChEBI	-	assimilation
[Ref.: #25177]	D-arabitol ChEBI	-	builds acid from
[Ref.: #25177]	D-fructose ChEBI	-	assimilation
[Ref.: #25177]	D-galactose ChEBI	-	assimilation
[Ref.: #25177]	D-glucose ChEBI	-	builds acid from
[Ref.: #25177]	D-glucose ChEBI	-	assimilation
[Ref.: #25177]	D-mannitol ChEBI	-	builds acid from
[Ref.: #25177]	D-mannitol ChEBI	-	assimilation
[Ref.: #25177]	D-mannose ChEBI	-	assimilation
[Ref.: #25177]	D-sorbitol ChEBI	-	builds acid from
[Ref.: #25177]	D-sorbitol ChEBI	-	assimilation
[Ref.: #25177]	D-xylose ChEBI	-	builds acid from
[Ref.: #25177]	D-xylose ChEBI	-	assimilation
[Ref.: #25177]	erythritol ChEBI	-	builds acid from
[Ref.: #25177]	galactitol ChEBI	-	builds acid from
[Ref.: #25177]	gamma-aminobutyric acid ChEBI	-	assimilation
[Ref.: #25177]	gelatin ChEBI	-	hydrolysis
[Ref.: #25177]	gluconate ChEBI	-	assimilation
[Ref.: #25177]	histidine ChEBI	-	assimilation
[Ref.: #25177]	itaconate ChEBI	-	assimilation
[Ref.: #25177]	L-arabinose ChEBI	-	builds acid from
[Ref.: #25177]	L-arabinose ChEBI	-	assimilation
[Ref.: #25177]	L-rhamnose ChEBI	-	builds acid from
[Ref.: #25177]	L-rhamnose ChEBI	-	assimilation
[Ref.: #25177]	lactose ChEBI	-	builds acid from
[Ref.: #25177]	malate ChEBI	-	assimilation
[Ref.: #25177]	maltitol ChEBI	-	assimilation
[Ref.: #25177]	maltose ChEBI	-	builds acid from
[Ref.: #25177]	maltose ChEBI	-	assimilation
[Ref.: #25177]	melibiose ChEBI	-	builds acid from
[Ref.: #25177]	melibiose ChEBI	-	assimilation
[Ref.: #25177]	mesaconate ChEBI	-	assimilation
[Ref.: #25177]	myo-inositol ChEBI	-	builds acid from
[Ref.: #25177]	myo-inositol ChEBI	-	assimilation
[Ref.: #25177]	N-acetylglucosamine ChEBI	-	assimilation
[Ref.: #25177]	propionate ChEBI	-	assimilation
[Ref.: #25177]	putrescine ChEBI	-	assimilation
[Ref.: #25177]	pyruvate ChEBI	-	assimilation
[Ref.: #25177]	raffinose ChEBI	-	builds acid from
[Ref.: #25177]	ribitol ChEBI	-	builds acid from
[Ref.: #25177]	ribose ChEBI	-	assimilation
[Ref.: #25177]	salicin ChEBI	-	builds acid from
[Ref.: #25177]	salicin ChEBI	-	assimilation
[Ref.: #25177]	serine ChEBI	-	assimilation
[Ref.: #25177]	starch ChEBI	-	hydrolysis
[Ref.: #25177]	sucrose ChEBI	-	builds acid from
[Ref.: #25177]	sucrose ChEBI	-	assimilation
[Ref.: #25177]	trans-aconitate ChEBI	-	assimilation
[Ref.: #25177]	trehalose ChEBI	-	builds acid from
[Ref.: #25177]	trehalose ChEBI	-	assimilation
[Ref.: #25177]	tryptophan ChEBI	-	energy source
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Production
[Ref.: #25177]	acetoin ChEBI	no
[Ref.: #25177]	hydrogen sulfide ChEBI	no
[Ref.: #25177]	indole ChEBI	no

	Metabolite	Voges-Proskauer-test	Indole test	Citrate test
[Ref.: #25177]	acetoin ChEBI	-
[Ref.: #25177]	citrate ChEBI			-
[Ref.: #25177]	indole ChEBI		-

	Enzyme	Enzyme activity	EC number
[Ref.: #25177]	arginine dihydrolase	-	3.5.3.6
[Ref.: #25177]	catalase	-	1.11.1.6
[Ref.: #25177]	cytochrome oxidase	+	1.9.3.1
[Ref.: #25177]	DNase	-
[Ref.: #25177]	gelatinase	-
[Ref.: #25177]	lysine decarboxylase	-	4.1.1.18
[Ref.: #25177]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #25177]	tryptophan deaminase	-	4.1.99.1
[Ref.: #25177]	urease	-	3.5.1.5

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #25177]

Fatty acid	Percentage
C15:0 anteiso	36.5
C16:0 iso	14.3
C16:0	11.8
C15:0 iso	9.7
C17:0 anteiso	5.6
C14:0	3
C15:0	2.6
C17:0 iso	2.4
C14:0 iso	2.1
C18:1ω9c	1.5

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #24859]	Sample type/isolated from	metalworking fluid, coolant lubricant
[Ref.: #24859]	Geographic location (country and/or sea, region)	Hessen, Gießen
[Ref.: #24859]	Country	Germany
[Ref.: #24859]	Country ISO 3 Code	DEU
[Ref.: #24859]	Continent	Europe

[Ref.: #25177]	Sample type/isolated from	coolant lubricant solution
[Ref.: #25177]	Enrichment culture	tryptone soy agar (TSA; Oxoid)
[Ref.: #25177]	Enrichment culture duration	1 day
[Ref.: #25177]	Enrichment culture temperature	28 ̊C
[Ref.: #25177]	Isolation/enrichment procedure	subcultivated

[Ref.: #120656]	Sample type/isolated from	Environment, Metalworking fluid
[Ref.: #120656]	Geographic location (country and/or sea, region)	Giessen, Hessen
[Ref.: #120656]	Country	Germany
[Ref.: #120656]	Country ISO 3 Code	DEU
[Ref.: #120656]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Engineered

#Industrial

#Engineered product

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #24859]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #120656]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #24859]	Cohnella lubricantis strain KSS-154-50 16S ribosomal RNA gene, partial sequence	KX581041	1467	ENA	2163172 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Cohnella lubricantis DSM 103658	GCA_017874835	contig	GenBank	2163172 tax ID	*
[Ref.: #66792]	Cohnella lubricantis DSM 103658	GCA_014212015	scaffold	GenBank	2163172 tax ID	*
[Ref.: #66792]	Cohnella lubricantis strain DSM 103658	2163172.3	wgs	PATRIC	2163172 tax ID	*
[Ref.: #66792]	Cohnella lubricantis strain DSM 103658	2163172.4	wgs	PATRIC	2163172 tax ID	*
[Ref.: #66792]	Cohnella lubricantis DSM 103658	2913573228	draft	JGI IMG/M	2163172 tax ID	*

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Cohnella lubricantis strain DSM 103658 PATRIC: 2163172.4
[Ref.: #69481]	spore-forming	yes	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Cohnella lubricantis DSM 103658 NCBI: GCA_014212015.1
[Ref.: #69480]	flagellated	yes	67.786	no
[Ref.: #69480]	gram-positive	yes	88.183	yes
[Ref.: #69480]	anaerobic	no	98.935	yes
[Ref.: #69480]	aerobic	yes	87.809	yes
[Ref.: #69480]	halophile	no	92.31	no
[Ref.: #69480]	spore-forming	yes	95.367	no
[Ref.: #69480]	glucose-util	yes	82.539	yes
[Ref.: #69480]	thermophile	no	93.377	no
[Ref.: #69480]	motile	yes	86.971	no
[Ref.: #69480]	glucose-ferment	no	89.94	no

Availability in culture collections External links

[Ref.: #24859]

Culture collection no.

DSM 103658, CCM 8707, LMG 29763, CIP 111180

[Ref.: #91336]

SI-ID 399652

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Cohnella lubricantis sp. nov., isolated from a coolant lubricant solution.	Kampfer P, Glaeser SP, Busse HJ	Int J Syst Evol Microbiol	10.1099/ijsem.0.001654	2017	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#24859

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 103658

#25177

P. G. Kämpfer, S. P.,Busse, H. J.: Cohnella lubricantis sp. nov., isolated from a coolant lubricant solution. IJSEM 67: 466 - 471 2017 ( DOI 10.1099/ijsem.0.001654 , PubMed 27902352 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#91336

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID399652.1 )

#120656 Collection of Institut Pasteur ; Curators of the CIP; CIP 111180
* These data were automatically processed and therefore are not curated

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Cohnella lubricantis strain DSM 103658

Cohnella lubricantis DSM 103658

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