Name: Rhodococcus sovatensis H004
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 133063

Type strain:

Species: Rhodococcus sovatensis

Strain Designation: H004

Culture col. no.: DSM 102881, NCAIM B.02632

Strain history: <- A. Táncsics, Szent István University, Hungary; H004 <- I. Máthé, Sapientia Hungarian University of Transylvania, Romania; H004

NCBI tax ID(s): 1805840 (species)

SI-ID 405667

DSM 102881

Section

Rhodococcus sovatensis H004 is an aerobe, Gram-positive, rod-shaped prokaryote that was isolated from water from a hypersaline and heliothermal lake.

Gram-positive
rod-shaped
aerobe
16S sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Genus	*Rhodococcus*
	Species	**Rhodococcus sovatensis**
	Full Scientific Name (LPSN)	Rhodococcus sovatensis Táncsics et al. 2017

Information on morphological and physiological properties Morphology

[Ref.: #25206]	Gram stain	positive

[Ref.: #25206]	Cell length	1.2-2.2 µm

[Ref.: #25206]	Cell shape	rod-shaped

[Ref.: #25206]	Motility	no

[Ref.: #25206]	Colony color	orange opaque
[Ref.: #25206]	Cultivation medium used	trypticase soy agar (TSA; BBL)

Multimedia content

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Caption:

Medium 215 28°C

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Intellectual property rights:

Information on culture and growth conditions Culture and growth conditions

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Culture medium

trypticase soy agar (TSA; BBL)

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Culture medium growth

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Culture medium

trypticase soy broth (TSB)

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Culture medium growth

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Culture medium

TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

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Culture medium composition

expand / minimize

	Kind of temperature		Temperature
[Ref.: #24851]		growth	28 °C
[Ref.: #25206]		growth	10-35 °C
[Ref.: #25206]		optimum	28-30 °C
[Ref.: #25206]	no	growth	37 °C

[Ref.: #24851]	Temperature range	mesophilic
[Ref.: #25206]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #25206]			optimum	7
[Ref.: #25206]			growth	6-10

Information on physiology and metabolism Physiology and metabolism

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Oxygen tolerance

aerobe

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #25206]		NaCl		resistant	0.0-9.0	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #25206]	D-arabitol ChEBI	+	assimilation
[Ref.: #25206]	D-fructose ChEBI	+	assimilation
[Ref.: #25206]	D-glucose ChEBI	+	assimilation
[Ref.: #25206]	D-mannitol ChEBI	+	assimilation
[Ref.: #25206]	D-mannose ChEBI	+	assimilation
[Ref.: #25206]	D-ribose ChEBI	+	assimilation
[Ref.: #25206]	D-sorbitol ChEBI	+	assimilation
[Ref.: #25206]	esculin ChEBI	-	hydrolysis
[Ref.: #25206]	glucose ChEBI	-	fermentation
[Ref.: #25206]	malate ChEBI	+	assimilation
[Ref.: #25206]	nitrate ChEBI	-	reduction
[Ref.: #25206]	nitrite ChEBI	-	reduction
[Ref.: #25206]	potassium gluconate ChEBI	+	assimilation
[Ref.: #25206]	salicin ChEBI	+	assimilation
[Ref.: #25206]	sodium citrate ChEBI	+	assimilation
[Ref.: #25206]	trehalose ChEBI	+	assimilation
[Ref.: #25206]	tween 80 ChEBI	-	hydrolysis
[Ref.: #25206]	xylitol ChEBI	+	assimilation
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Production
[Ref.: #25206]	acetoin ChEBI	no
[Ref.: #25206]	hydrogen sulfide ChEBI	yes
[Ref.: #25206]	indole ChEBI	no

	Physiological tests	Metabolite	Voges-Proskauer-test	Methylred-test
[Ref.: #25206]		acetoin ChEBI	-
[Ref.: #25206]		glucose ChEBI		-

	Enzyme	Enzyme activity	EC number
[Ref.: #25206]	acid phosphatase	-	3.1.3.2
[Ref.: #25206]	alkaline phosphatase	+	3.1.3.1
[Ref.: #25206]	alpha-chymotrypsin	-	3.4.21.1
[Ref.: #25206]	alpha-fucosidase	-	3.2.1.51
[Ref.: #25206]	alpha-galactosidase	-	3.2.1.22
[Ref.: #25206]	alpha-glucosidase	+	3.2.1.20
[Ref.: #25206]	alpha-mannosidase	-	3.2.1.24
[Ref.: #25206]	amylase	-
[Ref.: #25206]	beta-galactosidase	-	3.2.1.23
[Ref.: #25206]	beta-glucosidase	-	3.2.1.21
[Ref.: #25206]	beta-glucuronidase	-	3.2.1.31
[Ref.: #25206]	caseinase	-	3.4.21.50
[Ref.: #25206]	catalase	+	1.11.1.6
[Ref.: #25206]	cystine arylamidase	+	3.4.11.3
[Ref.: #25206]	cytochrome oxidase	-	1.9.3.1
[Ref.: #25206]	esterase (C 4)	+
[Ref.: #25206]	esterase Lipase (C 8)	+
[Ref.: #25206]	gelatinase	-
[Ref.: #25206]	leucine arylamidase	+	3.4.11.1
[Ref.: #25206]	lipase (C 14)	+
[Ref.: #25206]	N-acetyl-beta-glucosaminidase	-	3.2.1.52
[Ref.: #25206]	naphthol-AS-BI-phosphohydrolase	-
[Ref.: #25206]	trypsin	-	3.4.21.4
[Ref.: #25206]	urease	-	3.5.1.5
[Ref.: #25206]	valine arylamidase	+
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

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Fatty acid	Percentage
C15:0	24.4
C18:1ω9c	12.1
C19:1ω11c / C19:1ω9c	10.3
C16:1ω7c / iso-C15:02-OH	10.1
C16:0	8.6
C17:1ω8c	7.6
C18:0 10-methyl	6.2
C18:0	3.4
C15:1ω5c	3.2
C17:0	3
C14:0	2.9
C17:0 10-methyl	2.7
C13:0	1.9
C20:0	1.6

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #24851]	Sample type/isolated from	water from a hypersaline and heliothermal lake
[Ref.: #24851]	Geographic location (country and/or sea, region)	Sovata, Lake Ursu at a depth of 4 m
[Ref.: #24851]	Country	Romania
[Ref.: #24851]	Country ISO 3 Code	ROU
[Ref.: #24851]	Continent	Europe
[Ref.: #24851]	Geographic location	46.6028°/25.0864°

[Ref.: #25206]	Enrichment culture	Halomonas agar medium
[Ref.: #25206]	Enrichment culture duration	7 days
[Ref.: #25206]	Enrichment culture temperature	28 ̊C
[Ref.: #25206]	Isolation/enrichment procedure	standard dilution plating technique
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	#Lake (large)
#Condition	#Saline	-

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

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Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #24851]	Rhodococcus sovatensis strain H004 16S ribosomal RNA gene, partial sequence	KU189221	1414	ENA	1805840 tax ID

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GC-content

65.4 mol%

high performance liquid chromatography (HPLC)

Availability in culture collections External links

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Culture collection no.

DSM 102881, NCAIM B.02632

[Ref.: #91328]

SI-ID 405667

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Rhodococcus sovatensis sp. nov., an actinomycete isolated from the hypersaline and heliothermal Lake Ursu.	Tancsics A, Mathe I, Benedek T, Toth EM, Atasayar E, Sproer C, Marialigeti K, Felfoldi T, Kriszt B	Int J Syst Evol Microbiol	10.1099/ijsem.0.001514	2017	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#24851

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 102881

#25206

A. M. Táncsics, Istvan,Benedek, Tibor,Tóth, Erika M,Atasayar, Ewelina,Spröer, Cathrin,Márialigeti, Karoly,Felföldi, Tamas,Kriszt, Balazs: Rhodococcus sovatensis sp. nov., an actinomycete isolated from the hypersaline and heliothermal Lake Ursu. IJSEM 67: 190 - 196 2017 ( DOI 10.1099/ijsem.0.001514 , PubMed 27902266 )

#91328

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID405667.1 )

* These data were automatically processed and therefore are not curated

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