Name: Lentibacillus populi WD4L-1
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 133029

Type strain:

Species: Lentibacillus populi

Strain Designation: WD4L-1

Culture col. no.: DSM 101738, CGMCC 1.15454

Strain history: <- J. Gao, Beijing Academy of Agriculture and Forestry Science <- P. Sun, State Key Laboratory of Tree Geetics and Breeding, Beijing; WD4L-1

NCBI tax ID(s): 1827502 (species)

SI-ID 400808

DSM 101738

Section

Lentibacillus populi WD4L-1 is an aerobe, spore-forming, Gram-positive bacterium that forms circular colonies and was isolated from surface-sterilized stem tissue of a poplar tree.

colony-forming
Gram-positive
motile
rod-shaped
spore-forming
aerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Bacillaceae*
	Genus	*Lentibacillus*
	Species	**Lentibacillus populi**
	Full Scientific Name (LPSN)	Lentibacillus populi Sun et al. 2016

Information on morphological and physiological properties Morphology

[Ref.: #25092]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #25092]	Cell length	1.5-2.9 µm

[Ref.: #25092]	Cell width	0.4-0.6 µm

[Ref.: #25092]	Cell shape	rod-shaped

[Ref.: #25092]	Motility	yes
[Ref.: #69480]	Motility	yes Confidence in %96.129

[Ref.: #25092]	Flagellum arrangement	lophotrichous

[Ref.: #25092]	Colony size	1.0-1.5 mm
[Ref.: #25092]	Colony color	cream
[Ref.: #25092]	Colony shape	circular
[Ref.: #25092]	Incubation period	4 days
[Ref.: #25092]	Cultivation medium used	LB agar (lysogeny broth agar)

Information on culture and growth conditions Culture and growth conditions

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Culture medium

LB agar (lysogeny broth agar)

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Culture medium growth

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Culture medium

TSA (Trypticase soy agar;Difco)

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Culture medium growth

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Culture medium

BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

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Culture medium composition

expand / minimize

		Temperature
[Ref.: #24817]	growth	30 °C
[Ref.: #25092]	growth	15.0-50.0 °C
[Ref.: #25092]	optimum	30 °C

[Ref.: #24817]	Temperature range	mesophilic
[Ref.: #25092]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #25092]			optimum	7
[Ref.: #25092]			growth	6.0-9.0

Information on physiology and metabolism Physiology and metabolism

[Ref.: #25092]

Oxygen tolerance

aerobe

[Ref.: #25092]	Spore description	ellipsoidal,terminal
[Ref.: #25092]	Type of spore	endospore
[Ref.: #25092]	Ability of spore formation	yes
[Ref.: #69481]	Ability of spore formation	yes Confidence in %100
[Ref.: #69480]	Ability of spore formation	yes Confidence in %100

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #25092]		NaCl		growth	0.0-15.0	%(w/v)
[Ref.: #25092]		NaCl		optimum	7.0-8.0	%(w/v)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #25092]	acetate ChEBI	+	growth
[Ref.: #25092]	acetoacetate ChEBI	+	growth
[Ref.: #25092]	ammonium ChEBI	+	nitrogen source
[Ref.: #25092]	amygdalin ChEBI	+/-	fermentation
[Ref.: #25092]	arabinose ChEBI	-	fermentation
[Ref.: #25092]	aztreonam ChEBI	+	growth
[Ref.: #25092]	casein	-	hydrolysis
[Ref.: #25092]	citrate ChEBI	+	carbon source
[Ref.: #25092]	D-glucose ChEBI	+	builds acid from
[Ref.: #25092]	D-mannitol ChEBI	+	assimilation
[Ref.: #25092]	D-mannitol ChEBI	+	builds acid from
[Ref.: #25092]	D-mannose ChEBI	+	assimilation
[Ref.: #25092]	D-mannose ChEBI	+	builds acid from
[Ref.: #25092]	esculin ChEBI	+	hydrolysis
[Ref.: #25092]	gelatin ChEBI	+	hydrolysis
[Ref.: #25092]	glucose ChEBI	-	fermentation
[Ref.: #25092]	glucose ChEBI	+	assimilation
[Ref.: #25092]	inosine ChEBI	+	growth
[Ref.: #25092]	lithium chloride ChEBI	+	growth
[Ref.: #25092]	malate ChEBI	+	assimilation
[Ref.: #25092]	maltose ChEBI	-	assimilation
[Ref.: #25092]	mannitol ChEBI	-	fermentation
[Ref.: #25092]	melibiose ChEBI	+	fermentation
[Ref.: #25092]	nitrate ChEBI	+	reduction
[Ref.: #25092]	propionate ChEBI	+	growth
[Ref.: #25092]	rhamnose ChEBI	-	fermentation
[Ref.: #25092]	sodium butyrate ChEBI	+	growth
[Ref.: #25092]	sodium lactate ChEBI	+	growth
[Ref.: #25092]	sorbitol ChEBI	-	fermentation
[Ref.: #25092]	starch ChEBI	-	hydrolysis
[Ref.: #25092]	sucrose ChEBI	+	builds acid from
[Ref.: #25092]	sucrose ChEBI	+/-	fermentation
[Ref.: #25092]	tween 20 ChEBI	+	hydrolysis
[Ref.: #25092]	tween 40 ChEBI	+	growth
[Ref.: #25092]	tween 80 ChEBI	+	hydrolysis
[Ref.: #25092]	tyrosine ChEBI	-	hydrolysis
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #25092]		hydrogen sulfide ChEBI	no

	Physiological tests	Metabolite	Citrate test
[Ref.: #25092]		citrate ChEBI	+

	Enzyme	Enzyme activity	EC number
[Ref.: #25092]	acid phosphatase	+	3.1.3.2
[Ref.: #25092]	alkaline phosphatase	+	3.1.3.1
[Ref.: #25092]	alpha-chymotrypsin	+	3.4.21.1
[Ref.: #25092]	alpha-fucosidase	-	3.2.1.51
[Ref.: #25092]	alpha-galactosidase	-	3.2.1.22
[Ref.: #25092]	alpha-glucosidase	+	3.2.1.20
[Ref.: #25092]	alpha-mannosidase	-	3.2.1.24
[Ref.: #25092]	beta-glucosidase	-	3.2.1.21
[Ref.: #25092]	beta-glucuronidase	-	3.2.1.31
[Ref.: #25092]	catalase	+	1.11.1.6
[Ref.: #25092]	cystine arylamidase	+	3.4.11.3
[Ref.: #25092]	cytochrome oxidase	+	1.9.3.1
[Ref.: #25092]	esterase (C 4)	+
[Ref.: #25092]	esterase Lipase (C 8)	+
[Ref.: #25092]	gelatinase	+
[Ref.: #25092]	leucine arylamidase	+	3.4.11.1
[Ref.: #25092]	lipase (C 14)	-
[Ref.: #25092]	N-acetyl-beta-glucosaminidase	+	3.2.1.52
[Ref.: #25092]	naphthol-AS-BI-phosphohydrolase	+
[Ref.: #25092]	trypsin	-	3.4.21.4
[Ref.: #25092]	valine arylamidase	+
	Only first 10 entries are displayed. Click here to see all.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #24817]	Sample type/isolated from	surface-sterilized stem tissue of a poplar tree
[Ref.: #24817]	Geographic location (country and/or sea, region)	Heilongjiang province, Wudalianchi National Geopark
[Ref.: #24817]	Country	China
[Ref.: #24817]	Country ISO 3 Code	CHN
[Ref.: #24817]	Continent	Asia

[Ref.: #25092]	Enrichment culture	LB agar (lysogeny broth agar)
[Ref.: #25092]	Enrichment culture duration	3-7 days
* marker position based on {}

Isolation sources categories

#Host	#Plants	#Tree
#Host Body-Site	#Plant	#Stem (Branch)
#Host Body-Site	#Plant	#Sterilized plant part

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence KT981892 (>99% sequence identity) for Bacillaceae from Microbeatlas

Aquatic Samples	14
Soil Samples	68
Animal Samples	20
Plant Samples	29
Total Samples	131

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #24817]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #24817]	Lentibacillus populi strain WD4L-1 16S ribosomal RNA gene, partial sequence	KT981892	1469	ENA	1827502 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Lentibacillus populi strain CGMCC 1.15454	1827502.3	wgs	PATRIC	1827502 tax ID	*

[Ref.: #24817]

GC-content

36.9 mol%

thermal denaturation, midpoint method (Tm)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Lentibacillus populi strain CGMCC 1.15454 PATRIC: 1827502.3
[Ref.: #69481]	spore-forming	yes	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Lentibacillus populi strain CGMCC 1.15454 PATRIC: 1827502.3
[Ref.: #69480]	motile	yes	90.915	yes
[Ref.: #69480]	gram-positive	yes	91.434	yes
[Ref.: #69480]	anaerobic	no	99.131	yes
[Ref.: #69480]	aerobic	yes	86.193	yes
[Ref.: #69480]	halophile	yes	87.039	no
[Ref.: #69480]	spore-forming	yes	96.298	yes
[Ref.: #69480]	glucose-util	yes	90.384	yes
[Ref.: #69480]	flagellated	yes	88.704	yes
[Ref.: #69480]	thermophile	no	97.879	no
[Ref.: #69480]	glucose-ferment	no	83.206	no

Availability in culture collections External links

[Ref.: #24817]

Culture collection no.

DSM 101738, CGMCC 1.15454

[Ref.: #91299]

SI-ID 400808

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Whole-Genome Sequencing of Lentibacillus sp. Strain JNUCC-1, Isolated from Fermented Anchovy Sauce (Myeolchi Aekjeot).	Byun HD, Hyun CG	Microbiol Resour Announc	10.1128/MRA.01542-19	2020	*
Phylogeny	Lentibacillus alimentarius sp. nov., isolated from Myeolchi-jeotgal, a traditional Korean high-salt fermented anchovy.	Sundararaman A, Srinivasan S, Lee JH, Lee SS	Antonie Van Leeuwenhoek	10.1007/s10482-017-1006-4	2018	*
Phylogeny	Lentibacillus populi sp. nov., a moderately halophilic, endophytic bacterium isolated from a poplar tree, and emended description of the genus Lentibacillus.	Sun P, Gao JL, Mao XJ, Zhao XH, Sun JG, Lu M	Int J Syst Evol Microbiol	10.1099/ijsem.0.001508	2016	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#24817

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 101738

#25092

P. G. Sun, J. L.,Mao, X. J.,Zhao, X. H.,Sun, J. G.,Lu, M.: Lentibacillus populi sp. nov., a moderately halophilic, endophytic bacterium isolated from a poplar tree, and emended description of the genus Lentibacillus. IJSEM 66: 5281 - 5287 2016 ( DOI 10.1099/ijsem.0.001508 , PubMed 27652598 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#91299

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID400808.1 )

* These data were automatically processed and therefore are not curated

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Lentibacillus populi strain CGMCC 1.15454

Lentibacillus populi strain CGMCC 1.15454

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