Name: Peptococcus simiae M108
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 133028

Type strain:

Species: Peptococcus simiae

Strain Designation: M108

Culture col. no.: DSM 100347, VKM B-2932

Strain history: <- V. Shcherbakova, Russian Academy of Sciences, Inst. of Biochem. and Physiol., Moscow, Russia; M108 <- A.N. Shkoporov and B.A. Efimov, Russian Natl. Res. Med. Univ., Moscow

NCBI tax ID(s): 1643805 (species)

SI-ID 399113

DSM 100347

Section

Peptococcus simiae M108 is an obligate anaerobe, chemoorganoheterotroph, mesophilic bacterium that forms circular colonies and was isolated from faeces of healthy adult Macaca mulatta.

coccus-shaped
colony-forming
Gram-positive
chemoorganoheterotroph
mesophilic
obligate anaerobe
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Clostridia*
	Order	*Eubacteriales*
	Family	*Peptococcaceae*
	Genus	*Peptococcus*
	Species	**Peptococcus simiae**
	Full Scientific Name (LPSN)	Peptococcus simiae Shkoporov et al. 2016

Information on morphological and physiological properties Morphology

[Ref.: #25108]	Gram stain	positive

[Ref.: #25108]	Cell length	1.1-1.4 µm

[Ref.: #25108]	Cell shape	coccus-shaped

[Ref.: #25108]	Motility	no

[Ref.: #25108]	Type of hemolysis	gamma
[Ref.: #25108]	Colony size	0.25-0.55 mm
[Ref.: #25108]	Colony color	grey
[Ref.: #25108]	Colony shape	circular
[Ref.: #25108]	Incubation period	7 days
[Ref.: #25108]	Cultivation medium used	Columbia blood agar

Information on culture and growth conditions Culture and growth conditions

[Ref.: #25108]

Culture medium

Columbia blood agar

[Ref.: #25108]

Culture medium growth

[Ref.: #24816]

Culture medium

COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)

[Ref.: #24816]

Culture medium growth

[Ref.: #24816]

Culture medium link

Medium recipe at MediaDive

[Ref.: #24816]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #24816]			growth	37 °C

[Ref.: #24816]	Temperature range	mesophilic
[Ref.: #25108]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #25108]

Oxygen tolerance

obligate anaerobe

[Ref.: #25108]

Nutrition type

chemoorganoheterotroph

[Ref.: #25108]

Ability of spore formation

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #25108]		NaCl		maximum	4	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68380]	arginine ChEBI	-	hydrolysis	* from API rID32A
[Ref.: #25108]	cellobiose ChEBI	-	builds acid from
[Ref.: #25108]	D-glucose ChEBI	-	builds acid from
[Ref.: #25108]	D-mannitol ChEBI	-	builds acid from
[Ref.: #25108]	D-mannose ChEBI	-	builds acid from
[Ref.: #25108]	D-mannose ChEBI	-	fermentation
[Ref.: #68380]	D-mannose ChEBI	-	fermentation	* from API rID32A
[Ref.: #25108]	D-sorbitol ChEBI	-	builds acid from
[Ref.: #25108]	D-trehalose ChEBI	-	builds acid from
[Ref.: #25108]	D-xylose ChEBI	-	builds acid from
[Ref.: #25108]	elemental sulfur ChEBI	+	electron acceptor
[Ref.: #25108]	esculin ChEBI	-	hydrolysis
[Ref.: #25108]	ferric iron ChEBI	-	electron acceptor
[Ref.: #25108]	gelatin ChEBI	-	degradation
[Ref.: #25108]	glycerol ChEBI	-	builds acid from
[Ref.: #25108]	L-arabinose ChEBI	-	builds acid from
[Ref.: #68380]	L-glutamate ChEBI	-	degradation	* from API rID32A
[Ref.: #25108]	L-rhamnose ChEBI	-	builds acid from
[Ref.: #25108]	lactose ChEBI	-	builds acid from
[Ref.: #25108]	maltose ChEBI	-	builds acid from
[Ref.: #25108]	melezitose ChEBI	-	builds acid from
[Ref.: #25108]	nitrate ChEBI	-	electron acceptor
[Ref.: #68380]	nitrate ChEBI	-	reduction	* from API rID32A
[Ref.: #25108]	peptone	+	required for growth
[Ref.: #25108]	raffinose ChEBI	-	builds acid from
[Ref.: #25108]	raffinose ChEBI	-	fermentation
[Ref.: #68380]	raffinose ChEBI	-	fermentation	* from API rID32A
[Ref.: #25108]	salicin ChEBI	-	builds acid from
[Ref.: #25108]	sucrose ChEBI	-	builds acid from
[Ref.: #25108]	sulfate ChEBI	-	electron acceptor
[Ref.: #25108]	sulfite ChEBI	+	electron acceptor
[Ref.: #25108]	taurine ChEBI	+	electron acceptor
[Ref.: #25108]	thiosulfate ChEBI	+	electron acceptor
[Ref.: #25108]	tryptophan ChEBI	-	energy source
[Ref.: #68380]	tryptophan ChEBI	-	energy source	* from API rID32A
[Ref.: #68380]	urea ChEBI	-	hydrolysis	* from API rID32A
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Production
[Ref.: #25108]	hydrogen sulfide ChEBI	yes
[Ref.: #25108]	indole ChEBI	no
[Ref.: #68380]	indole ChEBI	no	* from API rID32A

	Physiological tests	Metabolite	Indole test
[Ref.: #25108]		indole ChEBI	-
[Ref.: #68380]		indole ChEBI	-	* from API rID32A

	Enzyme	Enzyme activity	EC number
[Ref.: #25108]	6-phospho-beta-galactosidase	-	3.2.1.85
[Ref.: #68380]	alanine arylamidase	-	3.4.11.2	* from API rID32A
[Ref.: #68380]	alkaline phosphatase	-	3.1.3.1	* from API rID32A
[Ref.: #25108]	alpha-arabinosidase	-	3.2.1.55
[Ref.: #68380]	alpha-arabinosidase	-	3.2.1.55	* from API rID32A
[Ref.: #25108]	alpha-fucosidase	-	3.2.1.51
[Ref.: #68380]	alpha-fucosidase	-	3.2.1.51	* from API rID32A
[Ref.: #25108]	alpha-galactosidase	-	3.2.1.22
[Ref.: #68380]	alpha-galactosidase	-	3.2.1.22	* from API rID32A
[Ref.: #25108]	alpha-glucosidase	-	3.2.1.20
[Ref.: #68380]	alpha-glucosidase	-	3.2.1.20	* from API rID32A
[Ref.: #25108]	arginine arylamidase	-
[Ref.: #68380]	arginine dihydrolase	-	3.5.3.6	* from API rID32A
[Ref.: #25108]	beta-galactosidase	-	3.2.1.23
[Ref.: #68380]	beta-galactosidase	-	3.2.1.23	* from API rID32A
[Ref.: #68380]	beta-Galactosidase 6-phosphate	-		* from API rID32A
[Ref.: #25108]	beta-glucosidase	-	3.2.1.21
[Ref.: #68380]	beta-glucosidase	-	3.2.1.21	* from API rID32A
[Ref.: #25108]	beta-glucuronidase	-	3.2.1.31
[Ref.: #68380]	beta-glucuronidase	-	3.2.1.31	* from API rID32A
[Ref.: #25108]	catalase	+	1.11.1.6
[Ref.: #25108]	cytochrome oxidase	-	1.9.3.1
[Ref.: #68380]	glutamate decarboxylase	-	4.1.1.15	* from API rID32A
[Ref.: #25108]	glutamyl-glutamate arylamidase	-
[Ref.: #68380]	glutamyl-glutamate arylamidase	-		* from API rID32A
[Ref.: #25108]	glycin arylamidase	-
[Ref.: #68380]	glycin arylamidase	-		* from API rID32A
[Ref.: #25108]	histidine arylamidase	-
[Ref.: #68380]	histidine arylamidase	-		* from API rID32A
[Ref.: #68380]	L-arginine arylamidase	-		* from API rID32A
[Ref.: #25108]	leucine arylamidase	-	3.4.11.1
[Ref.: #68380]	leucine arylamidase	-	3.4.11.1	* from API rID32A
[Ref.: #25108]	leucyl glycin arylamidase	-	3.4.11.1
[Ref.: #68380]	leucyl glycin arylamidase	-	3.4.11.1	* from API rID32A
[Ref.: #25108]	N-acetyl-beta-glucosaminidase	-	3.2.1.52
[Ref.: #68380]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API rID32A
[Ref.: #25108]	nitrate reductase	-	1.7.99.4
[Ref.: #25108]	phenylalanine arylamidase	-
[Ref.: #68380]	phenylalanine arylamidase	-		* from API rID32A
[Ref.: #25108]	proline-arylamidase	-	3.4.11.5
[Ref.: #68380]	proline-arylamidase	-	3.4.11.5	* from API rID32A
[Ref.: #25108]	pyroglutamic acid arylamidase	-
[Ref.: #68380]	pyrrolidonyl arylamidase	-	3.4.19.3	* from API rID32A
[Ref.: #25108]	serine arylamidase	-
[Ref.: #68380]	serine arylamidase	-		* from API rID32A
[Ref.: #25108]	tryptophan deaminase	-	4.1.99.1
[Ref.: #68380]	tryptophan deaminase	-	4.1.99.1	* from API rID32A
[Ref.: #25108]	tyrosine arylamidase	-
[Ref.: #68380]	tyrosine arylamidase	-		* from API rID32A
[Ref.: #25108]	urease	-	3.5.1.5
[Ref.: #68380]	urease	-	3.5.1.5	* from API rID32A
	Only first 10 entries are displayed. Click here to see all.

API® rID32A:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Urease/urea hydrolysisURE	Arginine dihydrolaseADH (Arg)	alpha-Galactosidasealpha GAL	beta-Galactosidasebeta GAL	beta-Galactosidase 6-phosphatebeta GP	alpha-Glucosidasealpha GLU	beta-Glucosidasebeta GLU	alpha-Arabinosidasealpha ARA	beta-Glucuronidasebeta GUR	beta-N-Acetyl-beta-glucosaminidasebeta NAG	Fermentation of D-mannoseMNE	Fermentation of raffinoseRAF	Glutamate decarboxylaseGDC	alpha-Fucosidasealpha FUC	Reduction of nitrateNIT	Indole productionIND	Alkaline phosphatasePAL	L-arginine arylamidaseArgA	Proline arylamidaseProA	Leucyl glycin arylamidaseLGA	Phenylalanine arylamidasePheA	Leucine arylamidaseLeuA	Pyrrolidonyl arylamidasePyrA	Tyrosine arylamidaseTyrA	Alanine arylamidaseAlaA	Glycin arylamidaseGlyA	Histidine arylamidaseHisA	Glutamyl-glutamate arylamidaseGGA	Serine arylamidaseSerA
[Ref.: #24816]	12881	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #24816]	Sample type/isolated from	faeces of healthy adult Macaca mulatta
[Ref.: #24816]	Host species	Macaca mulatta
[Ref.: #24816]	Geographic location (country and/or sea, region)	Rijswijk
[Ref.: #24816]	Country	Netherlands
[Ref.: #24816]	Country ISO 3 Code	NLD
[Ref.: #24816]	Continent	Europe

[Ref.: #25108]	Sample type/isolated from	faecal samples from three healthy female rhe- sus macaques (Macaca mulatta)
[Ref.: #25108]	Enrichment culture	Clostridium perfringens TSC agar (Himedia)
[Ref.: #25108]	Enrichment culture duration	3 days
[Ref.: #25108]	Enrichment culture temperature	37 ̊C
* marker position based on {}

Isolation sources categories

#Infection	#Patient	-
#Host	#Mammals	#Primates
#Host Body Product	#Gastrointestinal tract	#Feces (Stool)
#Host	#Human	#Female

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence KP322014 (>99% sequence identity) for Peptococcus simiae subclade from Microbeatlas

Aquatic Samples
Soil Samples	2
Animal Samples	199
Plant Samples
Total Samples	201

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #24816]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #24816]	Peptococcus simiae strain M108 16S ribosomal RNA gene, partial sequence	KP322014	1488	ENA	1643805 tax ID

[Ref.: #25108]	GC-content	51 mol%
[Ref.: #24816]	GC-content	51.0 mol%	high performance liquid chromatography (HPLC)

Availability in culture collections External links

[Ref.: #24816]

Culture collection no.

DSM 100347, VKM B-2932

[Ref.: #91298]

SI-ID 399113

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Peptococcus simiae sp. nov., isolated from rhesus macaque faeces and emended description of the genus Peptococcus.	Shkoporov AN, Efimov BA, Kondova I, Ouwerling B, Chaplin AV, Shcherbakova VA, Langermans JAM	Int J Syst Evol Microbiol	10.1099/ijsem.0.001494	2016	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#24816

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 100347

#25108

A. N. E. Shkoporov, B. A.,Kondova, I.,Ouwerling, B.,Chaplin, A. V.,Shcherbakova, V. A.,Langermans, J. A.: Peptococcus simiae sp. nov., isolated from rhesus macaque faeces and emended description of the genus Peptococcus. IJSEM 66: 5187 - 5191 2016 ( DOI 10.1099/ijsem.0.001494 , PubMed 27613234 )

#68380

Automatically annotated from API rID32A .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#91298

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID399113.1 )

* These data were automatically processed and therefore are not curated

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