Name: Nakamurella silvestris S20-107
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 133020

Type strain:

Species: Nakamurella silvestris

Strain Designation: S20-107

Culture col. no.: DSM 102309, LMG 29427

Strain history: <- R. Margesin, Inst. Microbiology, Univ. Innsbruck, Austria; S20-107

NCBI tax ID(s): 1645681 (species)

SI-ID 406432

DSM 102309

Section

Nakamurella silvestris S20-107 is an aerobe, chemoorganotroph, Gram-positive bacterium that forms circular colonies and was isolated from alpine forest soil.

colony-forming
Gram-positive
rod-shaped
aerobe
chemoorganotroph
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Nakamurellales*
	Family	*Nakamurellaceae*
	Genus	*Nakamurella*
	Species	**Nakamurella silvestris**
	Full Scientific Name (LPSN)	Nakamurella silvestris França et al. 2016

Information on morphological and physiological properties Morphology

[Ref.: #25063]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #25063]	Cell length	2.1-2.6 µm

[Ref.: #25063]	Cell width	0.8-0.9 µm

[Ref.: #25063]	Cell shape	rod-shaped

[Ref.: #25063]	Motility	no

[Ref.: #25063]	Colony size	0.6 mm
[Ref.: #25063]	Colony color	creamy white
[Ref.: #25063]	Colony shape	circular
[Ref.: #25063]	Incubation period	4 days
[Ref.: #25063]	Cultivation medium used	R2A agar (Reasoner's 2A agar)

Information on culture and growth conditions Culture and growth conditions

[Ref.: #25063]

Culture medium

Reasoner's 2A agar (R2A)

[Ref.: #25063]

Culture medium growth

[Ref.: #25063]

Culture medium

NA (Nutrient Agar)

[Ref.: #25063]

Culture medium growth

[Ref.: #25063]

Culture medium

TSA (Trypticase soy agar)

[Ref.: #25063]

Culture medium growth

[Ref.: #24808]

Culture medium

TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)

[Ref.: #24808]

Culture medium growth

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Culture medium link

Medium recipe at MediaDive

[Ref.: #24808]

Culture medium composition

expand / minimize

	Kind of temperature		Temperature
[Ref.: #24808]		growth	28 °C
[Ref.: #25063]		growth	0.0-30.0 °C
[Ref.: #25063]		optimum	20.0-30.0 °C
[Ref.: #25063]	no	growth	35 °C

[Ref.: #24808]	Temperature range	mesophilic
[Ref.: #25063]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #25063]			optimum	6.0-7.0
[Ref.: #25063]			growth	6.0-9.0

Information on physiology and metabolism Physiology and metabolism

[Ref.: #25063]	Oxygen tolerance	aerobe
[Ref.: #25063]	Oxygen tolerance	microaerophile

[Ref.: #25063]

Nutrition type

chemoorganotroph

[Ref.: #25063]	Ability of spore formation	no
[Ref.: #69481]	Ability of spore formation	no Confidence in %98
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.974

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #25063]		NaCl		optimum	0.0-1.0	%(w/v)
[Ref.: #25063]		NaCl		growth	0.0-3.0	%(w/v)

[Ref.: #25063]	Murein short key	A31
[Ref.: #25063]	Murein types	A1gamma m-Dpm-direct

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #25063]	adipate ChEBI	-	assimilation
[Ref.: #25063]	carboxymethylcellulose ChEBI	-	hydrolysis
[Ref.: #25063]	D-glucose ChEBI	+	assimilation
[Ref.: #25063]	D-mannitol ChEBI	-	assimilation
[Ref.: #25063]	D-mannose ChEBI	+	assimilation
[Ref.: #25063]	decanoate ChEBI	-	assimilation
[Ref.: #25063]	esculin ChEBI	+	hydrolysis
[Ref.: #25063]	L-arabinose ChEBI	+	assimilation
[Ref.: #25063]	malate ChEBI	-	assimilation
[Ref.: #25063]	maltose ChEBI	+	assimilation
[Ref.: #25063]	N-acetylglucosamine ChEBI	+	assimilation
[Ref.: #25063]	nitrate ChEBI	-	reduction
[Ref.: #25063]	phenylacetate ChEBI	-	assimilation
[Ref.: #25063]	potassium gluconate ChEBI	+	assimilation
[Ref.: #25063]	skimmed milk	-	hydrolysis
[Ref.: #25063]	sodium citrate ChEBI	-	assimilation
[Ref.: #25063]	starch ChEBI	+	hydrolysis
[Ref.: #25063]	tryptophan ChEBI	-	energy source
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #25063]		indole ChEBI	no

	Physiological tests	Metabolite	Indole test
[Ref.: #25063]		indole ChEBI	-

	Enzyme	Enzyme activity	EC number
[Ref.: #25063]	acid phosphatase	+	3.1.3.2
[Ref.: #25063]	alpha-chymotrypsin	-	3.4.21.1
[Ref.: #25063]	alpha-fucosidase	-	3.2.1.51
[Ref.: #25063]	alpha-galactosidase	-	3.2.1.22
[Ref.: #25063]	alpha-glucosidase	+	3.2.1.20
[Ref.: #25063]	alpha-mannosidase	-	3.2.1.24
[Ref.: #25063]	arginine dihydrolase	-	3.5.3.6
[Ref.: #25063]	beta-galactosidase	+	3.2.1.23
[Ref.: #25063]	beta-glucosidase	+	3.2.1.21
[Ref.: #25063]	beta-glucuronidase	-	3.2.1.31
[Ref.: #25063]	catalase	+	1.11.1.6
[Ref.: #25063]	cystine arylamidase	+	3.4.11.3
[Ref.: #25063]	cytochrome oxidase	-	1.9.3.1
[Ref.: #25063]	esterase Lipase (C 8)	+
[Ref.: #25063]	gelatinase	-
[Ref.: #25063]	leucine arylamidase	+	3.4.11.1
[Ref.: #25063]	lipase (C 14)	-
[Ref.: #25063]	N-acetyl-beta-glucosaminidase	+	3.2.1.52
[Ref.: #25063]	naphthol-AS-BI-phosphohydrolase	+
[Ref.: #25063]	pyrazinamidase	+	3.5.1.B15
[Ref.: #25063]	pyrrolidonyl arylamidase	-	3.4.19.3
[Ref.: #25063]	trypsin	-	3.4.21.4
[Ref.: #25063]	tryptophan deaminase	-	4.1.99.1
[Ref.: #25063]	urease	+	3.5.1.5
	Only first 10 entries are displayed. Click here to see all.

	Pathway	Enzyme coverage	Annotated reactions
	Pathway annotation	Computationally determined by
[Ref.: #66794]	acetate fermentation	100	4 of 4
[Ref.: #66794]	cis-vaccenate biosynthesis	100	2 of 2
[Ref.: #66794]	cardiolipin biosynthesis	100	7 of 7
[Ref.: #66794]	threonine metabolism	100	10 of 10
[Ref.: #66794]	aspartate and asparagine metabolism	100	9 of 9
[Ref.: #66794]	palmitate biosynthesis	100	22 of 22
[Ref.: #66794]	ppGpp biosynthesis	100	4 of 4
[Ref.: #66794]	adipate degradation	100	2 of 2
[Ref.: #66794]	methylglyoxal degradation	100	5 of 5
[Ref.: #66794]	valine metabolism	100	9 of 9
[Ref.: #66794]	starch degradation	100	10 of 10
[Ref.: #66794]	lipoate biosynthesis	100	5 of 5
[Ref.: #66794]	propanol degradation	100	7 of 7
[Ref.: #66794]	3-chlorocatechol degradation	100	5 of 5
[Ref.: #66794]	UDP-GlcNAc biosynthesis	100	3 of 3
[Ref.: #66794]	folate polyglutamylation	100	1 of 1
[Ref.: #66794]	CDP-diacylglycerol biosynthesis	100	2 of 2
[Ref.: #66794]	coenzyme A metabolism	100	4 of 4
[Ref.: #66794]	formaldehyde oxidation	100	3 of 3
[Ref.: #66794]	vitamin K metabolism	100	5 of 5
[Ref.: #66794]	suberin monomers biosynthesis	100	2 of 2
[Ref.: #66794]	cellulose degradation	100	5 of 5
[Ref.: #66794]	NAD metabolism	100	18 of 18
[Ref.: #66794]	anapleurotic synthesis of oxalacetate	100	1 of 1
[Ref.: #66794]	glutamate and glutamine metabolism	89.29	25 of 28
[Ref.: #66794]	molybdenum cofactor biosynthesis	88.89	8 of 9
[Ref.: #66794]	chorismate metabolism	88.89	8 of 9
[Ref.: #66794]	serine metabolism	88.89	8 of 9
[Ref.: #66794]	allantoin degradation	88.89	8 of 9
[Ref.: #66794]	ketogluconate metabolism	87.5	7 of 8
[Ref.: #66794]	gluconeogenesis	87.5	7 of 8
[Ref.: #66794]	peptidoglycan biosynthesis	86.67	13 of 15
[Ref.: #66794]	pyrimidine metabolism	86.67	39 of 45
[Ref.: #66794]	reductive acetyl coenzyme A pathway	85.71	6 of 7
[Ref.: #66794]	photosynthesis	85.71	12 of 14
[Ref.: #66794]	leucine metabolism	84.62	11 of 13
[Ref.: #66794]	phenylalanine metabolism	84.62	11 of 13
[Ref.: #66794]	glycolate and glyoxylate degradation	83.33	5 of 6
[Ref.: #66794]	vitamin B12 metabolism	82.35	28 of 34
[Ref.: #66794]	glycolysis	82.35	14 of 17
[Ref.: #66794]	purine metabolism	81.91	77 of 94
[Ref.: #66794]	pentose phosphate pathway	81.82	9 of 11
[Ref.: #66794]	flavin biosynthesis	80	12 of 15
[Ref.: #66794]	factor 420 biosynthesis	80	4 of 5
[Ref.: #66794]	Entner Doudoroff pathway	80	8 of 10
[Ref.: #66794]	glycogen metabolism	80	4 of 5
[Ref.: #66794]	propionate fermentation	80	8 of 10
[Ref.: #66794]	alanine metabolism	79.31	23 of 29
[Ref.: #66794]	tetrahydrofolate metabolism	78.57	11 of 14
[Ref.: #66794]	heme metabolism	78.57	11 of 14
[Ref.: #66794]	citric acid cycle	78.57	11 of 14
[Ref.: #66794]	CO2 fixation in Crenarchaeota	77.78	7 of 9
[Ref.: #66794]	d-mannose degradation	77.78	7 of 9
[Ref.: #66794]	sulfopterin metabolism	75	3 of 4
[Ref.: #66794]	glycogen biosynthesis	75	3 of 4
[Ref.: #66794]	dTDPLrhamnose biosynthesis	75	6 of 8
[Ref.: #66794]	butanoate fermentation	75	3 of 4
[Ref.: #66794]	isoleucine metabolism	75	6 of 8
[Ref.: #66794]	C4 and CAM-carbon fixation	75	6 of 8
[Ref.: #66794]	tryptophan metabolism	73.68	28 of 38
[Ref.: #66794]	metabolism of disaccharids	72.73	8 of 11
[Ref.: #66794]	proline metabolism	72.73	8 of 11
[Ref.: #66794]	histidine metabolism	72.41	21 of 29
[Ref.: #66794]	glutathione metabolism	71.43	10 of 14
[Ref.: #66794]	ubiquinone biosynthesis	71.43	5 of 7
[Ref.: #66794]	lipid metabolism	70.97	22 of 31
[Ref.: #66794]	oxidative phosphorylation	70.33	64 of 91
[Ref.: #66794]	urea cycle	69.23	9 of 13
[Ref.: #66794]	degradation of sugar alcohols	68.75	11 of 16
[Ref.: #66794]	non-pathway related	68.42	26 of 38
[Ref.: #66794]	enterobactin biosynthesis	66.67	2 of 3
[Ref.: #66794]	octane oxidation	66.67	2 of 3
[Ref.: #66794]	cyanate degradation	66.67	2 of 3
[Ref.: #66794]	L-lactaldehyde degradation	66.67	2 of 3
[Ref.: #66794]	degradation of hexoses	66.67	12 of 18
[Ref.: #66794]	IAA biosynthesis	66.67	2 of 3
[Ref.: #66794]	sulfoquinovose degradation	66.67	2 of 3
[Ref.: #66794]	acetyl CoA biosynthesis	66.67	2 of 3
[Ref.: #66794]	acetoin degradation	66.67	2 of 3
[Ref.: #66794]	degradation of pentoses	64.29	18 of 28
[Ref.: #66794]	d-xylose degradation	63.64	7 of 11
[Ref.: #66794]	vitamin B6 metabolism	63.64	7 of 11
[Ref.: #66794]	arginine metabolism	62.5	15 of 24
[Ref.: #66794]	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	62.5	5 of 8
[Ref.: #66794]	methionine metabolism	61.54	16 of 26
[Ref.: #66794]	isoprenoid biosynthesis	61.54	16 of 26
[Ref.: #66794]	cysteine metabolism	61.11	11 of 18
[Ref.: #66794]	4-hydroxyphenylacetate degradation	60	6 of 10
[Ref.: #66794]	metabolism of amino sugars and derivatives	60	3 of 5
[Ref.: #66794]	glycine betaine biosynthesis	60	3 of 5
[Ref.: #66794]	lysine metabolism	59.52	25 of 42
[Ref.: #66794]	degradation of aromatic, nitrogen containing compounds	58.33	7 of 12
[Ref.: #66794]	degradation of sugar acids	56	14 of 25
[Ref.: #66794]	4-hydroxymandelate degradation	55.56	5 of 9
[Ref.: #66794]	coenzyme M biosynthesis	50	5 of 10
[Ref.: #66794]	phenylmercury acetate degradation	50	1 of 2
[Ref.: #66794]	biotin biosynthesis	50	2 of 4
[Ref.: #66794]	ascorbate metabolism	50	11 of 22
[Ref.: #66794]	glycine metabolism	50	5 of 10
[Ref.: #66794]	kanosamine biosynthesis II	50	1 of 2
[Ref.: #66794]	ethanol fermentation	50	1 of 2
[Ref.: #66794]	mannosylglycerate biosynthesis	50	1 of 2
[Ref.: #66794]	CMP-KDO biosynthesis	50	2 of 4
[Ref.: #66794]	tyrosine metabolism	50	7 of 14
[Ref.: #66794]	aminopropanol phosphate biosynthesis	50	1 of 2
[Ref.: #66794]	sulfate reduction	46.15	6 of 13
[Ref.: #66794]	phenol degradation	45	9 of 20
[Ref.: #66794]	lipid A biosynthesis	44.44	4 of 9
[Ref.: #66794]	arachidonic acid metabolism	44.44	8 of 18
[Ref.: #66794]	aclacinomycin biosynthesis	42.86	3 of 7
[Ref.: #66794]	benzoyl-CoA degradation	42.86	3 of 7
[Ref.: #66794]	phenylacetate degradation (aerobic)	40	2 of 5
[Ref.: #66794]	D-cycloserine biosynthesis	40	2 of 5
[Ref.: #66794]	arachidonate biosynthesis	40	2 of 5
[Ref.: #66794]	myo-inositol biosynthesis	40	4 of 10
[Ref.: #66794]	ethylmalonyl-CoA pathway	40	2 of 5
[Ref.: #66794]	gallate degradation	40	2 of 5
[Ref.: #66794]	3-phenylpropionate degradation	40	6 of 15
[Ref.: #66794]	phosphatidylethanolamine bioynthesis	38.46	5 of 13
[Ref.: #66794]	vitamin B1 metabolism	38.46	5 of 13
[Ref.: #66794]	carnitine metabolism	37.5	3 of 8
[Ref.: #66794]	bile acid biosynthesis, neutral pathway	35.29	6 of 17
[Ref.: #66794]	selenocysteine biosynthesis	33.33	2 of 6
[Ref.: #66794]	nitrate assimilation	33.33	3 of 9
[Ref.: #66794]	(5R)-carbapenem carboxylate biosynthesis	33.33	1 of 3
[Ref.: #66794]	pantothenate biosynthesis	33.33	2 of 6
[Ref.: #66794]	sphingosine metabolism	33.33	2 of 6
[Ref.: #66794]	androgen and estrogen metabolism	31.25	5 of 16
[Ref.: #66794]	phenylpropanoid biosynthesis	30.77	4 of 13
[Ref.: #66794]	dolichyl-diphosphooligosaccharide biosynthesis	27.27	3 of 11
[Ref.: #66794]	polyamine pathway	26.09	6 of 23
[Ref.: #66794]	cyclohexanol degradation	25	1 of 4
[Ref.: #66794]	lactate fermentation	25	1 of 4
[Ref.: #66794]	catecholamine biosynthesis	25	1 of 4
[Ref.: #66794]	toluene degradation	25	1 of 4
[Ref.: #66794]	chlorophyll metabolism	22.22	4 of 18
		Only first 10 entries are displayed. Click here to see all.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #24808]	Sample type/isolated from	alpine forest soil
[Ref.: #24808]	Geographic location (country and/or sea, region)	South Tyrol, Schwarzseespitze/Ritten
[Ref.: #24808]	Country	Italy
[Ref.: #24808]	Country ISO 3 Code	ITA
[Ref.: #24808]	Continent	Europe

[Ref.: #25063]	Enrichment culture	R2A agar (Reasoner's 2A agar)
[Ref.: #25063]	Enrichment culture temperature	20 ̊C
* marker position based on {}

Isolation sources categories

#Climate	#Cold	#Alpine
#Environmental	#Terrestrial	#Forest
#Environmental	#Terrestrial	#Soil

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence KP899234 (>99% sequence identity) for Nakamurella from Microbeatlas

Aquatic Samples	19
Soil Samples	62
Animal Samples	113
Plant Samples	117
Total Samples	311

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #24808]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #24808]	Nakamurella silvestris strain S20-107 16S ribosomal RNA gene, partial sequence	KP899234	1344	ENA	1645681 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Nakamurella silvestris DSM 102309	GCA_003576535	scaffold	GenBank	1645681 tax ID	*
[Ref.: #66792]	Nakamurella silvestris strain DSM 102309	1645681.3	wgs	PATRIC	1645681 tax ID	*
[Ref.: #66792]	Nakamurella silvestris DSM 102309	2833868136	draft	JGI IMG/M	1645681 tax ID	*

[Ref.: #25063]	GC-content	69.1-71.9 mol%	high performance liquid chromatography (HPLC)
[Ref.: #24808]	GC-content	70.5 mol%	high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Nakamurella silvestris DSM 102309 NCBI: GCA_003576535.1
[Ref.: #69481]	spore-forming	no	98	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Nakamurella silvestris DSM 102309 NCBI: GCA_003576535.1
[Ref.: #69480]	motile	no	91.606	no
[Ref.: #69480]	flagellated	no	98.224	yes
[Ref.: #69480]	gram-positive	yes	88.253	yes
[Ref.: #69480]	anaerobic	no	99.108	yes
[Ref.: #69480]	aerobic	yes	93.844	no
[Ref.: #69480]	halophile	no	94.713	no
[Ref.: #69480]	spore-forming	no	83.821	yes
[Ref.: #69480]	glucose-util	yes	86.813	yes
[Ref.: #69480]	thermophile	no	98.558	yes
[Ref.: #69480]	glucose-ferment	no	88.857	no

Availability in culture collections External links

[Ref.: #24808]

Culture collection no.

DSM 102309, LMG 29427

[Ref.: #91294]

SI-ID 406432

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Nakamurella deserti sp. nov., isolated from rhizosphere soil of Reaumuria in the Taklamakan desert.	Liu SW, Li FN, Qi X, Xie YY, Sun CH	Int J Syst Evol Microbiol	10.1099/ijsem.0.003132	2018	*
Phylogeny	Nakamurella silvestris sp. nov., an actinobacterium isolated from alpine forest soil.	Franca L, Albuquerque L, Zhang DC, Nouioui I, Klenk HP, da Costa MS, Margesin R	Int J Syst Evol Microbiol	10.1099/ijsem.0.001541	2016	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#24808

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 102309

#25063

L. A. Franca, L.,Zhang, D. C.,Nouioui, I.,Klenk, H. P.,da Costa, M. S.,Margesin, R.: Nakamurella silvestris sp. nov., an actinobacterium isolated from alpine forest soil. IJSEM 66: 5460 - 5464 2016 ( DOI 10.1099/ijsem.0.001541 , PubMed 27707433 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#66794

Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#91294

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID406432.1 )

* These data were automatically processed and therefore are not curated

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Nakamurella silvestris DSM 102309

Nakamurella silvestris DSM 102309

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