Name: Ancylomarina subtilis FA102
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 133003

Type strain:

Species: Ancylomarina subtilis

Strain Designation: FA102

Culture col. no.: DSM 28825, CICC 10902, KCTC 42257

Strain history: <- Zong-Jun Du, Shandong Univ., China

NCBI tax ID(s): 1639035 (species)

SI-ID 399341

DSM 28825

Section

Ancylomarina subtilis FA102 is a facultative anaerobe, moderately halophilic, Gram-negative bacterium that forms circular colonies and was isolated from marine sediment.

colony-forming
filament-shaped
Gram-negative
facultative anaerobe
moderately halophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacteroidota*
	Class	*Bacteroidia*
	Order	*Bacteroidales*
	Family	*Marinifilaceae*
	Genus	*Ancylomarina*
	Species	**Ancylomarina subtilis**
	Full Scientific Name (LPSN)	Ancylomarina subtilis Wu et al. 2016

Information on morphological and physiological properties Morphology

[Ref.: #24917]	Gram stain	negative
[Ref.: #67771]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %100

[Ref.: #24917]	Cell length	2.9-30.0 µm

[Ref.: #24917]	Cell width	0.3-0.4 µm

[Ref.: #24917]	Cell shape	filament-shaped
[Ref.: #67771]	Cell shape	filament-shaped

[Ref.: #24917]	Motility	no
[Ref.: #67771]	Motility	no

[Ref.: #24917]	Colony size	1.0-1.5 mm
[Ref.: #24917]	Colony color	beige
[Ref.: #24917]	Colony shape	circular
[Ref.: #24917]	Incubation period	2-3 days
[Ref.: #24917]	Cultivation medium used	2216E agar (Hopebio)

Information on culture and growth conditions Culture and growth conditions

[Ref.: #24917]

Culture medium

2216E agar (Hopebio)

[Ref.: #24917]

Culture medium growth

[Ref.: #24791]

Culture medium

BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)

[Ref.: #24791]

Culture medium growth

[Ref.: #24791]

Culture medium link

Medium recipe at MediaDive

[Ref.: #24791]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #24791]	growth	28 °C
[Ref.: #24917]	growth	8.0-33.0 °C
[Ref.: #24917]	optimum	28.0-30.0 °C
[Ref.: #67771]	growth	28-30 °C

[Ref.: #24791]	Temperature range	mesophilic
[Ref.: #24917]	Temperature range	mesophilic
[Ref.: #67771]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #24917]			optimum	7.5
[Ref.: #24917]			growth	6.0-8.5

Information on physiology and metabolism Physiology and metabolism

[Ref.: #24917]	Oxygen tolerance	facultative anaerobe
[Ref.: #67771]	Oxygen tolerance	facultative anaerobe

[Ref.: #24917]	Ability of spore formation	no
[Ref.: #69481]	Ability of spore formation	no Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.998

[Ref.: #24917]

Halophily / tolerance level

moderately halophilic

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #24917]		NaCl		growth	0.5-5.0	%(w/v)
[Ref.: #24917]		NaCl		optimum	2	%(w/v)

[Ref.: #67771]

Observation

quinones: MK-7

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #24917]	5-dehydro-D-gluconate ChEBI	+	builds acid from
[Ref.: #24917]	agar ChEBI	+	hydrolysis
[Ref.: #24917]	alginate ChEBI	+	hydrolysis
[Ref.: #24917]	carboxymethylcellulose ChEBI	-	hydrolysis
[Ref.: #24917]	cellobiose ChEBI	-	builds acid from
[Ref.: #24917]	citrate ChEBI	-	carbon source
[Ref.: #24917]	D-galactose ChEBI	+	builds acid from
[Ref.: #24917]	D-glucose ChEBI	+	builds acid from
[Ref.: #24917]	esculin ChEBI	-	builds acid from
[Ref.: #24917]	esculin ChEBI	+	hydrolysis
[Ref.: #24917]	gelatin ChEBI	+	hydrolysis
[Ref.: #24917]	glucuronamide ChEBI	+	oxidation
[Ref.: #24917]	L-malate ChEBI	+	oxidation
[Ref.: #24917]	N-acetylglucosamine ChEBI	+	builds acid from
[Ref.: #24917]	nitrate ChEBI	-	reduction
[Ref.: #24917]	O-nitrophenyl-beta-D-galactopyranosid	+	hydrolysis
[Ref.: #24917]	starch ChEBI	-	hydrolysis
[Ref.: #24917]	tween 80 ChEBI	-	hydrolysis
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Production
[Ref.: #24917]	acetoin ChEBI	no
[Ref.: #24917]	hydrogen sulfide ChEBI	no
[Ref.: #24917]	indole ChEBI	yes

	Physiological tests	Metabolite	Voges-Proskauer-test	Citrate test
[Ref.: #24917]		acetoin ChEBI	-
[Ref.: #24917]		citrate ChEBI		-

	Enzyme	Enzyme activity	EC number
[Ref.: #24917]	acid phosphatase	+	3.1.3.2
[Ref.: #24917]	alkaline phosphatase	+	3.1.3.1
[Ref.: #24917]	alpha-galactosidase	-	3.2.1.22
[Ref.: #24917]	alpha-glucosidase	-	3.2.1.20
[Ref.: #24917]	alpha-mannosidase	-	3.2.1.24
[Ref.: #24917]	arginine dihydrolase	-	3.5.3.6
[Ref.: #24917]	beta-D-fucosidase	-	3.2.1.38
[Ref.: #24917]	beta-galactosidase	-	3.2.1.23
[Ref.: #24917]	beta-glucosidase	-	3.2.1.21
[Ref.: #24917]	beta-glucuronidase	+	3.2.1.31
[Ref.: #24917]	beta-glucuronidase	-	3.2.1.31
[Ref.: #24917]	catalase	-	1.11.1.6
[Ref.: #24917]	chymotrypsin	+	3.4.4.5
[Ref.: #24917]	cystine arylamidase	-	3.4.11.3
[Ref.: #24917]	cytochrome oxidase	-	1.9.3.1
[Ref.: #24917]	esterase (C 4)	+
[Ref.: #24917]	esterase lipase (C 8)	-
[Ref.: #24917]	gelatinase	+
[Ref.: #24917]	leucine arylamidase	-	3.4.11.1
[Ref.: #24917]	lipase (C 14)	-
[Ref.: #24917]	lysine decarboxylase	-	4.1.1.18
[Ref.: #24917]	N-acetyl-beta-glucosaminidase	+	3.2.1.52
[Ref.: #24917]	naphthol-AS-BI-phosphohydrolase	+
[Ref.: #24917]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #24917]	trypsin	-	3.4.21.4
[Ref.: #24917]	tryptophan deaminase	-	4.1.99.1
[Ref.: #24917]	urease	-	3.5.1.5
[Ref.: #24917]	valine arylamidase	-
	Only first 10 entries are displayed. Click here to see all.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #24791]	Sample type/isolated from	marine sediment
[Ref.: #24791]	Geographic location (country and/or sea, region)	coast of Weihai (37° 32' 01.93'' N 122° 03' 44.01'' E)
[Ref.: #24791]	Country	China
[Ref.: #24791]	Country ISO 3 Code	CHN
[Ref.: #24791]	Continent	Asia
[Ref.: #24791]	Geographic location	37.5339°/122.0620°

[Ref.: #67771]	Sample type/isolated from	From marine sediment
[Ref.: #67771]	Geographic location (country and/or sea, region)	coaste of Weihai
[Ref.: #67771]	Country	China
[Ref.: #67771]	Country ISO 3 Code	CHN
[Ref.: #67771]	Continent	Asia

[Ref.: #24917]	Enrichment culture duration	5 days
[Ref.: #24917]	Enrichment culture temperature	25 ̊C
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	#Marine
#Environmental	#Aquatic	#Sediment

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence KP214056 (>99% sequence identity) for Ancylomarina subtilis subclade from Microbeatlas

Aquatic Samples	226
Soil Samples	18
Animal Samples	7
Plant Samples	10
Total Samples	261

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #24791]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #24791]	Ancylomarina subtilis strain FA102 16S ribosomal RNA gene, partial sequence	KP214056	1450	ENA	1639035 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Ancylomarina subtilis DSM 28825	GCA_004217115	scaffold	GenBank	1639035 tax ID	*
[Ref.: #66792]	Ancylomarina subtilis strain DSM 28825	1639035.3	wgs	PATRIC	1639035 tax ID	*
[Ref.: #66792]	Ancylomarina subtilis DSM 28825	2795386138	draft	JGI IMG/M	1639035 tax ID	*

[Ref.: #24791]	GC-content	36.5 mol%	high performance liquid chromatography (HPLC)
[Ref.: #67771]	GC-content	36.5 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Ancylomarina subtilis DSM 28825 NCBI: GCA_004217115.1
[Ref.: #69481]	spore-forming	no	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Ancylomarina subtilis DSM 28825 NCBI: GCA_004217115.1
[Ref.: #69480]	flagellated	no	90.368	no
[Ref.: #69480]	gram-positive	no	97.972	yes
[Ref.: #69480]	anaerobic	no	54.632	yes
[Ref.: #69480]	aerobic	no	95.326	yes
[Ref.: #69480]	halophile	yes	66.663	yes
[Ref.: #69480]	spore-forming	no	92.707	yes
[Ref.: #69480]	glucose-util	yes	80.448	no
[Ref.: #69480]	thermophile	no	99.26	no
[Ref.: #69480]	motile	no	88.658	yes
[Ref.: #69480]	glucose-ferment	no	58.881	no

Availability in culture collections External links

[Ref.: #24791]

Culture collection no.

DSM 28825, CICC 10902, KCTC 42257

[Ref.: #91279]

SI-ID 399341

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Ancylomarina psychrotolerans sp. nov., isolated from sediments of Fildes Peninsula and emended the description of genus Ancylomarina.	Jia C, Cui HC, Han YQ, Fu TY, Du R, Wang XL, Shi XC, Zhang XH	Antonie Van Leeuwenhoek	10.1007/s10482-018-1025-9	2018	*
Phylogeny	Description of Ancylomarina subtilis gen. nov., sp. nov., isolated from coastal sediment, proposal of Marinilabiliales ord. nov. and transfer of Marinilabiliaceae, Prolixibacteraceae and Marinifilaceae to the order Marinilabiliales.	Wu WJ, Zhao JX, Chen GJ, Du ZJ	Int J Syst Evol Microbiol	10.1099/ijsem.0.001342	2016	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#24791

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 28825

#24917

W.-J. Z. Wu, Jin-Xin,Chen, Guan-Jun,Du, Zong-Jun: Description of Ancylomarina subtilis gen. nov., sp. nov., isolated from coastal sediment, proposal of Marinilabiliales ord. nov. and transfer of Marinilabiliaceae, Prolixibacteraceae and Marinifilaceae to the order Marinilabiliales. IJSEM 66: 4243 - 4249 2016 ( DOI 10.1099/ijsem.0.001342 , PubMed 27470589 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67771 Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#91279

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID399341.1 )

* These data were automatically processed and therefore are not curated

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Ancylomarina subtilis DSM 28825

Ancylomarina subtilis DSM 28825

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