Name: Chenggangzhangella methanolivorans DSM 100641
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 132989

Type strain:

Species: Chenggangzhangella methanolivorans

Culture col. no.: DSM 100641, CCTCC AB 2015175, KCTC 42661, CHL 1

Strain history: <- L. Yang, CAS; CHL1

NCBI tax ID(s): 1437009 (species)

SI-ID 400585

DSM 100641

Section

Chenggangzhangella methanolivorans DSM 100641 is an aerobe, Gram-negative, rod-shaped bacterium that forms circular colonies and was isolated from sludge from a sewage treatment tank at an agricultural chemical factory.

colony-forming
Gram-negative
rod-shaped
aerobe
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Alphaproteobacteria*
	Order	*Hyphomicrobiales*
	Family	*Methylocystaceae*
	Genus	*Chenggangzhangella*
	Species	**Chenggangzhangella methanolivorans**
	Full Scientific Name (LPSN)	Chenggangzhangella methanolivorans Yang et al. 2016

Information on morphological and physiological properties Morphology

[Ref.: #43584]	Gram stain	negative

[Ref.: #43584]	Cell length	1.2-1.8 µm

[Ref.: #43584]	Cell width	0.9-1 µm

[Ref.: #43584]	Cell shape	rod-shaped

[Ref.: #43584]	Motility	no

[Ref.: #43584]	Colony size	1-2 mm
[Ref.: #43584]	Colony color	White
[Ref.: #43584]	Colony shape	circular
[Ref.: #43584]	Incubation period	3 days
[Ref.: #43584]	Cultivation medium used	PBMM agar

Information on culture and growth conditions Culture and growth conditions

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Culture medium

PBMM agar

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Culture medium growth

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Culture medium

R2A MEDIUM (DSMZ Medium 830)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

[Ref.: #24777]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #24777]	growth	28 °C
[Ref.: #43584]	growth	5-40 °C
[Ref.: #43584]	optimum	28-30 °C

	pH	Kind of pH		pH
[Ref.: #43584]			optimum	7
[Ref.: #43584]			growth	6-8

Information on physiology and metabolism Physiology and metabolism

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Oxygen tolerance

aerobe

[Ref.: #43584]	Type of spore	endospore
[Ref.: #43584]	Ability of spore formation	no

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #43584]		NaCl		growth	0.5-5	%(w/v)
[Ref.: #43584]		NaCl		optimum	2.5	%(w/v)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #43584]	(S)-lactate ChEBI	-	carbon source
[Ref.: #43584]	2-hydroxybutyrate ChEBI	-	carbon source
[Ref.: #43584]	2-oxobutanoate ChEBI	-	carbon source
[Ref.: #43584]	acetate ChEBI	+	carbon source
[Ref.: #43584]	acetoacetate ChEBI	-	carbon source
[Ref.: #43584]	adipate ChEBI	+	assimilation
[Ref.: #43584]	alpha-D-glucose ChEBI	-	carbon source
[Ref.: #43584]	alpha-lactose ChEBI	-	carbon source
[Ref.: #43584]	bromosuccinate ChEBI	+	carbon source
[Ref.: #43584]	cellobiose ChEBI	-	carbon source
[Ref.: #43584]	citrate ChEBI	-	carbon source
[Ref.: #43584]	D-arabitol ChEBI	-	carbon source
[Ref.: #43584]	D-fructose ChEBI	-	carbon source
[Ref.: #43584]	D-fucose ChEBI	-	carbon source
[Ref.: #43584]	D-galactose ChEBI	-	carbon source
[Ref.: #43584]	D-gluconate ChEBI	+	carbon source
[Ref.: #43584]	D-glucose ChEBI	-	assimilation
[Ref.: #43584]	D-glucose ChEBI	+	builds acid from
[Ref.: #43584]	D-malate ChEBI	+	carbon source
[Ref.: #43584]	D-mannitol ChEBI	-	assimilation
[Ref.: #43584]	D-mannitol ChEBI	-	carbon source
[Ref.: #43584]	D-mannose ChEBI	-	assimilation
[Ref.: #43584]	D-mannose ChEBI	-	carbon source
[Ref.: #43584]	D-saccharate ChEBI	+	carbon source
[Ref.: #43584]	D-sorbitol ChEBI	-	carbon source
[Ref.: #43584]	decanoate ChEBI	+	assimilation
[Ref.: #43584]	dextrin ChEBI	-	carbon source
[Ref.: #43584]	esculin ChEBI	-	hydrolysis
[Ref.: #43584]	formate ChEBI	+	carbon source
[Ref.: #43584]	gamma-aminobutyric acid ChEBI	-	carbon source
[Ref.: #43584]	gelatin ChEBI	-	carbon source
[Ref.: #43584]	gelatin ChEBI	-	hydrolysis
[Ref.: #43584]	gentiobiose ChEBI	-	carbon source
[Ref.: #43584]	glucuronamide ChEBI	+	carbon source
[Ref.: #43584]	glycerol ChEBI	-	carbon source
[Ref.: #43584]	inosine ChEBI	-	carbon source
[Ref.: #43584]	L-arabinose ChEBI	+	assimilation
[Ref.: #43584]	L-aspartate ChEBI	+	carbon source
[Ref.: #43584]	L-fucose ChEBI	-	carbon source
[Ref.: #43584]	L-glutamate ChEBI	+	carbon source
[Ref.: #43584]	L-rhamnose ChEBI	-	carbon source
[Ref.: #43584]	malate ChEBI	+	assimilation
[Ref.: #43584]	maltose ChEBI	-	carbon source
[Ref.: #43584]	maltose ChEBI	+	assimilation
[Ref.: #43584]	melibiose ChEBI	-	carbon source
[Ref.: #43584]	methyl pyruvate ChEBI	+	carbon source
[Ref.: #43584]	myo-inositol ChEBI	+	carbon source
[Ref.: #43584]	N-acetylglucosamine ChEBI	-	assimilation
[Ref.: #43584]	nitrate ChEBI	-	reduction
[Ref.: #43584]	pectin ChEBI	-	carbon source
[Ref.: #43584]	phenylacetate ChEBI	-	assimilation
[Ref.: #43584]	potassium gluconate ChEBI	-	assimilation
[Ref.: #43584]	propionate ChEBI	-	carbon source
[Ref.: #43584]	raffinose ChEBI	-	carbon source
[Ref.: #43584]	sodium citrate ChEBI	-	assimilation
[Ref.: #43584]	stachyose ChEBI	-	carbon source
[Ref.: #43584]	sucrose ChEBI	-	carbon source
[Ref.: #43584]	trehalose ChEBI	-	carbon source
[Ref.: #43584]	turanose ChEBI	-	carbon source
[Ref.: #43584]	tween 20 ChEBI	-	hydrolysis
[Ref.: #43584]	tween 40 ChEBI	-	hydrolysis
[Ref.: #43584]	tween 60 ChEBI	+	hydrolysis
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #43584]		hydrogen sulfide ChEBI	no
[Ref.: #43584]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #43584]	acid phosphatase	+	3.1.3.2
[Ref.: #43584]	alkaline phosphatase	+	3.1.3.1
[Ref.: #43584]	alpha-chymotrypsin	-	3.4.21.1
[Ref.: #43584]	alpha-galactosidase	-	3.2.1.22
[Ref.: #43584]	alpha-glucosidase	-	3.2.1.20
[Ref.: #43584]	alpha-mannosidase	-	3.2.1.24
[Ref.: #43584]	amylase	-
[Ref.: #43584]	arginine dihydrolase	-	3.5.3.6
[Ref.: #43584]	beta-D-fucosidase	-	3.2.1.38
[Ref.: #43584]	beta-galactosidase	-	3.2.1.23
[Ref.: #43584]	beta-glucosidase	-	3.2.1.21
[Ref.: #43584]	beta-glucuronidase	-	3.2.1.31
[Ref.: #43584]	catalase	+	1.11.1.6
[Ref.: #43584]	cellulase	-	3.2.1.4
[Ref.: #43584]	cystine arylamidase	+	3.4.11.3
[Ref.: #43584]	cytochrome oxidase	+	1.9.3.1
[Ref.: #43584]	esterase (C 4)	+
[Ref.: #43584]	esterase Lipase (C 8)	+
[Ref.: #43584]	leucine arylamidase	+	3.4.11.1
[Ref.: #43584]	lipase (C 14)	+
[Ref.: #43584]	N-acetyl-beta-glucosaminidase	-	3.2.1.52
[Ref.: #43584]	naphthol-AS-BI-phosphohydrolase	+
[Ref.: #43584]	trypsin	+	3.4.21.4
[Ref.: #43584]	urease	-	3.5.1.5
[Ref.: #43584]	valine arylamidase	+
	Only first 10 entries are displayed. Click here to see all.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #24777]	Sample type/isolated from	sludge from a sewage treatment tank at an agricultural chemical factory
[Ref.: #24777]	Geographic location (country and/or sea, region)	Jiangsu Province
[Ref.: #24777]	Country	China
[Ref.: #24777]	Country ISO 3 Code	CHN
[Ref.: #24777]	Continent	Asia

[Ref.: #43584]	Sample type/isolated from	a sludge sample collected from a sewage treatment tank at an agricultural chemical factory
[Ref.: #43584]	Geographic location (country and/or sea, region)	Jiangsu Provicne
[Ref.: #43584]	Country	China
[Ref.: #43584]	Country ISO 3 Code	CHN
[Ref.: #43584]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Engineered	#Agriculture	-
#Engineered	#Waste	#Wastewater
#Environmental	#Terrestrial	#Mud (Sludge)
#Engineered	#Built environment	#Container (Reservoir)
#Engineered	#Industrial	#Plant (Factory)
#Engineered	#Waste	#Sewage sludge

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence KF726142 (>99% sequence identity) for Chenggangzhangella methanolivorans subclade from Microbeatlas

Aquatic Samples	37
Soil Samples	10
Animal Samples	45
Plant Samples	13
Total Samples	105

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #24777]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #24777]	Chenggangzhangella methanolivorans strain CHL1 16S ribosomal RNA gene, partial sequence	KF726142	1448	GenBank ENA	1437009 tax ID

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GC-content

70.4 mol%

high performance liquid chromatography (HPLC)

Availability in culture collections External links

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Culture collection no.

DSM 100641, CCTCC AB 2015175, KCTC 42661, CHL 1

[Ref.: #91265]

SI-ID 400585

Literature:

Topic	Title	Authors	Journal	DOI	Year
Metabolism	Dynamic changes in microbial communities during the bioremediation of herbicide (chlorimuron-ethyl and atrazine) contaminated soils by combined degrading bacteria.	Wang J, Li X, Li X, Wang H, Su Z, Wang X, Zhang H	PLoS One	10.1371/journal.pone.0194753	2018	*
Phylogeny	Chenggangzhangella methanolivorans gen. nov., sp. nov., a member of the family Methylocystaceae, transfer of Methylopila helvetica Doronina et al. 2000 to Albibacter helveticus comb. nov. and emended description of the genus Albibacter.	Yang LQ, Liu L, Salam N, Xiao M, Kim CJ, Hozzein WN, Park DJ, Li WJ, Zhang HW	Int J Syst Evol Microbiol	10.1099/ijsem.0.001062	2016	*
Metabolism	A Novel Pathway of Chlorimuron-Ethyl Biodegradation by Chenggangzhangella methanolivorans Strain CHL1 and Its Molecular Mechanisms.	Yu Z, Dai Y, Li T, Gu W, Yang Y, Li X, Peng P, Yang L, Li X, Wang J, Su Z, Li X, Xu M, Zhang H	Int J Mol Sci	10.3390/ijms23179890	2022	*
Metabolism	Whole-Genome Sequencing of a Chlorimuron-Ethyl-Degrading Strain: Chenggangzhangella methanolivorans CHL1 and Its Degrading Enzymes.	Yu Z, Gu W, Yang Y, Li X, Li X, Li T, Wang J, Su Z, Li X, Dai Y, Xu M, Zhang H	Microbiol Spectr	10.1128/spectrum.01822-22	2022	*
	High bioremediation potential of strain Chenggangzhangella methanolivorans CHL1 for soil polluted with metsulfuron-methyl or tribenuron-methyl in a pot experiment.	Yang TT, Zhang HW, Wang J, Li XY, Li X, Su ZC	Environ Sci Pollut Res Int	10.1007/s11356-020-10825-w	2020	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#24777

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 100641

#43584

Li-Qiang Yang, Lan Liu, Nimaichand Salam, Min Xiao, Chang-Jin Kim, Wael N. Hozzein, Dong-Jin Park, Wen-Jun Li, Hui-Wen Zhang: Chenggangzhangella methanolivorans gen. nov., sp. nov., a member of the family Methylocystaceae, transfer of Methylopila helvetica Doronina et al. 2000 to Albibacter helveticus comb. nov. and emended description of the genus Albibacter. IJSEM 66: 2825 - 2830 2016 ( DOI 10.1099/ijsem.0.001062 , PubMed 27046027 )

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#91265

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID400585.1 )

* These data were automatically processed and therefore are not curated

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