Name: Hallella colorans A1336
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 132987

Type strain:

Species: Hallella colorans

Strain Designation: A1336

Culture col. no.: DSM 100333, CCOS 902, CCUG 67421, KCTC 15694

Strain history: <- DSM <- M Buhl, Univ. Tubingen, A1336

NCBI tax ID(s): 1703337 (species)

SI-ID 397050

DSM 100333, CCUG 67421

Section

Hallella colorans A1336 is an obligate anaerobe, mesophilic, Gram-negative human pathogen that forms circular colonies and was isolated from human wound swab; mixed infection of a 77 year old female patient.

colony-forming
Gram-negative
pleomorphic-shaped
human pathogen
mesophilic
obligate anaerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacteroidota*
	Class	*Bacteroidia*
	Order	*Bacteroidales*
	Family	*Prevotellaceae*
	Genus	*Hallella*
	Species	**Hallella colorans**
	Full Scientific Name (LPSN)	Hallella colorans (Buhl et al. 2016) Hitch et al. 2023

Synonym

Prevotella colorans

Information on morphological and physiological properties Morphology

[Ref.: #43096]	Gram stain	negative
[Ref.: #67771]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %99.999

[Ref.: #43096]	Cell length	0.9-1.3 µm

[Ref.: #43096]	Cell width	0.4-0.6 mm

[Ref.: #43096]	Cell shape	pleomorphic-shaped
[Ref.: #67771]	Cell shape	rod-shaped
[Ref.: #67771]	Cell shape	pleomorphic-shaped

[Ref.: #43096]	Motility	no
[Ref.: #67771]	Motility	no
[Ref.: #69480]	Motility	no Confidence in %93.558

[Ref.: #43096]	Colony size	1 mm
[Ref.: #43096]	Colony color	greyish-brown
[Ref.: #43096]	Colony shape	circular
[Ref.: #43096]	Incubation period	2-5 days
[Ref.: #43096]	Cultivation medium used	BHI agar

[Ref.: #63417]	Incubation period	2 days

Information on culture and growth conditions Culture and growth conditions

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Culture medium

BHI agar

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Culture medium growth

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Culture medium composition

expand / minimize

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Culture medium

chocolate agar

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Culture medium growth

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Culture medium

Schädler agar

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Culture medium growth

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Culture medium

liver broth

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Culture medium growth

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Culture medium

CMC broth

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Culture medium growth

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Culture medium

FASTIDIOUS ANAEROBE BROTH (DSMZ Medium 1203a)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

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Culture medium composition

expand / minimize

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Culture medium

COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

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Culture medium composition

expand / minimize

	Kind of temperature		Temperature
[Ref.: #24775]		growth	37 °C
[Ref.: #43096]		optimum	35-37 °C
[Ref.: #43096]	no	growth	45 °C
[Ref.: #43096]		growth	30-37 °C
[Ref.: #63417]		growth	37 °C
[Ref.: #67771]		growth	37 °C

[Ref.: #24775]	Temperature range	mesophilic
[Ref.: #43096]	Temperature range	mesophilic
[Ref.: #43096]	Temperature range	thermophilic
[Ref.: #43096]	Temperature range	mesophilic
[Ref.: #63417]	Temperature range	mesophilic
[Ref.: #67771]	Temperature range	mesophilic

	Kind of pH		pH
[Ref.: #43096]		optimum	7-9
[Ref.: #43096]		growth	6
[Ref.: #43096]	no	growth	5

Information on physiology and metabolism Physiology and metabolism

[Ref.: #43096]	Oxygen tolerance	obligate anaerobe
[Ref.: #63417]	Oxygen tolerance	anaerobe
[Ref.: #67771]	Oxygen tolerance	anaerobe
[Ref.: #69480]	Oxygen tolerance	anaerobe Confidence in %99.998

[Ref.: #43096]	Ability of spore formation	no
[Ref.: #67771]	Ability of spore formation	no
[Ref.: #69481]	Ability of spore formation	no Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.999

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #43096]	arabinose ChEBI	-	fermentation
[Ref.: #68380]	arginine ChEBI	-	hydrolysis	* from API rID32A
[Ref.: #43096]	cellobiose ChEBI	+	fermentation
[Ref.: #68380]	D-mannose ChEBI	-	fermentation	* from API rID32A
[Ref.: #43096]	glucose ChEBI	+	fermentation
[Ref.: #68380]	L-glutamate ChEBI	-	degradation	* from API rID32A
[Ref.: #43096]	lactose ChEBI	+	fermentation
[Ref.: #43096]	maltose ChEBI	+	fermentation
[Ref.: #43096]	mannitol ChEBI	-	fermentation
[Ref.: #43096]	mannose ChEBI	+	fermentation
[Ref.: #43096]	melezitose ChEBI	-	fermentation
[Ref.: #68380]	nitrate ChEBI	-	reduction	* from API rID32A
[Ref.: #43096]	raffinose ChEBI	+	fermentation
[Ref.: #68380]	raffinose ChEBI	-	fermentation	* from API rID32A
[Ref.: #43096]	salicin ChEBI	-	fermentation
[Ref.: #43096]	sorbitol ChEBI	-	fermentation
[Ref.: #43096]	sucrose ChEBI	+	fermentation
[Ref.: #43096]	trehalose ChEBI	-	fermentation
[Ref.: #68380]	tryptophan ChEBI	-	energy source	* from API rID32A
[Ref.: #68380]	urea ChEBI	-	hydrolysis	* from API rID32A
[Ref.: #43096]	xylose ChEBI	-	fermentation
	Only first 10 entries are displayed. Click here to see all.

	Antibiotica	Metabolite	Antibiotic resistance	Concentration
[Ref.: #43096]		colistin ChEBI	yes	10	µg (disc)
[Ref.: #43096]		vancomycin ChEBI	yes	5	µg (disc)

	Metabolite production	Metabolite	Production
[Ref.: #43096]		indole ChEBI	no
[Ref.: #68380]		indole ChEBI	no	* from API rID32A

	Physiological tests	Metabolite	Indole test
[Ref.: #68380]		indole ChEBI	-	* from API rID32A

	Enzyme	Enzyme activity	EC number
[Ref.: #43096]	6-phospho-beta-galactosidase	-	3.2.1.85
[Ref.: #43096]	alanine arylamidase	+	3.4.11.2
[Ref.: #68380]	alanine arylamidase	+	3.4.11.2	* from API rID32A
[Ref.: #43096]	alkaline phosphatase	+	3.1.3.1
[Ref.: #68380]	alkaline phosphatase	+	3.1.3.1	* from API rID32A
[Ref.: #43096]	alpha-arabinosidase	-	3.2.1.55
[Ref.: #68380]	alpha-arabinosidase	-	3.2.1.55	* from API rID32A
[Ref.: #68380]	alpha-fucosidase	-	3.2.1.51	* from API rID32A
[Ref.: #68380]	alpha-galactosidase	-	3.2.1.22	* from API rID32A
[Ref.: #43096]	alpha-glucosidase	+	3.2.1.20
[Ref.: #68380]	alpha-glucosidase	+	3.2.1.20	* from API rID32A
[Ref.: #43096]	arginine arylamidase	+
[Ref.: #68380]	arginine dihydrolase	-	3.5.3.6	* from API rID32A
[Ref.: #43096]	beta-galactosidase	+	3.2.1.23
[Ref.: #68380]	beta-galactosidase	+	3.2.1.23	* from API rID32A
[Ref.: #68380]	beta-Galactosidase 6-phosphate	-		* from API rID32A
[Ref.: #43096]	beta-glucosidase	-	3.2.1.21
[Ref.: #68380]	beta-glucosidase	-	3.2.1.21	* from API rID32A
[Ref.: #68380]	beta-glucuronidase	-	3.2.1.31	* from API rID32A
[Ref.: #43096]	catalase	-	1.11.1.6
[Ref.: #68380]	glutamate decarboxylase	-	4.1.1.15	* from API rID32A
[Ref.: #43096]	glutamyl-glutamate arylamidase	+
[Ref.: #68380]	glutamyl-glutamate arylamidase	+		* from API rID32A
[Ref.: #68380]	glycin arylamidase	-		* from API rID32A
[Ref.: #68380]	histidine arylamidase	-		* from API rID32A
[Ref.: #68380]	leucine arylamidase	-	3.4.11.1	* from API rID32A
[Ref.: #43096]	leucyl glycin arylamidase	+	3.4.11.1
[Ref.: #68380]	leucyl glycin arylamidase	+	3.4.11.1	* from API rID32A
[Ref.: #43096]	N-acetyl-beta-glucosaminidase	+	3.2.1.52
[Ref.: #68380]	N-acetyl-beta-glucosaminidase	+	3.2.1.52	* from API rID32A
[Ref.: #68380]	phenylalanine arylamidase	-		* from API rID32A
[Ref.: #68380]	proline-arylamidase	-	3.4.11.5	* from API rID32A
[Ref.: #68380]	pyrrolidonyl arylamidase	-	3.4.19.3	* from API rID32A
[Ref.: #68380]	serine arylamidase	+		* from API rID32A
[Ref.: #68380]	tryptophan deaminase	-	4.1.99.1	* from API rID32A
[Ref.: #68380]	tyrosine arylamidase	-		* from API rID32A
[Ref.: #68380]	urease	-	3.5.1.5	* from API rID32A
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #43096]

Fatty acid	Percentage
C17:0 iso 3OH	15.5
C15:0 anteiso	15.2
C15:0 iso	12.4

API® rID32A:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Urease/urea hydrolysisURE	Arginine dihydrolaseADH (Arg)	alpha-Galactosidasealpha GAL	beta-Galactosidasebeta GAL	beta-Galactosidase 6-phosphatebeta GP	alpha-Glucosidasealpha GLU	beta-Glucosidasebeta GLU	alpha-Arabinosidasealpha ARA	beta-Glucuronidasebeta GUR	beta-N-Acetyl-beta-glucosaminidasebeta NAG	Fermentation of D-mannoseMNE	Fermentation of raffinoseRAF	Glutamate decarboxylaseGDC	alpha-Fucosidasealpha FUC	Reduction of nitrateNIT	Indole productionIND	Alkaline phosphatasePAL	L-arginine arylamidaseArgA	Proline arylamidaseProA	Leucyl glycin arylamidaseLGA	Phenylalanine arylamidasePheA	Leucine arylamidaseLeuA	Pyrrolidonyl arylamidasePyrA	Tyrosine arylamidaseTyrA	Alanine arylamidaseAlaA	Glycin arylamidaseGlyA	Histidine arylamidaseHisA	Glutamyl-glutamate arylamidaseGGA	Serine arylamidaseSerA
[Ref.: #24775]	12490	-	-	-	+	-	+	-	-	-	+	-	-	-	-	-	-	+	+/-	-	+	-	-	-	-	+	-	-	+	+

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #24775]	Sample type/isolated from	human wound swab; mixed infection of a 77 year old female patient
[Ref.: #24775]	Country	Germany
[Ref.: #24775]	Country ISO 3 Code	DEU
[Ref.: #24775]	Continent	Europe

[Ref.: #43096]	Sample type/isolated from	wound of a 77 year old female patient
[Ref.: #43096]	Enrichment culture	brain-heart infusion (BHI) agar (Oxoid)
[Ref.: #43096]	Enrichment culture composition	supplemented with 5 % defibrinated sheep blood and IsoVitalex enrichment (BD)
[Ref.: #43096]	Enrichment culture temperature	37 ̊C

[Ref.: #63417]	Sample type/isolated from	Human wound
[Ref.: #63417]	Sampling date	2012-02-09
[Ref.: #63417]	Geographic location (country and/or sea, region)	Tübingen Univ Hospital
[Ref.: #63417]	Country	Germany
[Ref.: #63417]	Country ISO 3 Code	DEU
[Ref.: #63417]	Continent	Europe

[Ref.: #67771]	Sample type/isolated from	From human wound swab, mixed infection of a 77 year old female patient
[Ref.: #67771]	Country	Germany
[Ref.: #67771]	Country ISO 3 Code	DEU
[Ref.: #67771]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Infection	#Disease	-
#Infection	#Patient	#Swab
#Host	#Human	#Female
#Host Body-Site	#Other	#Wound

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #24775]	Pathogenicity (human)	yes, in single cases
[Ref.: #24775]	Biosafety level	1	Risk group (German classification) According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #24775]	Prevotella colorans strain A1336 16S ribosomal RNA gene, partial sequence	KT886066	1491	ENA	1703337 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67771]	Hallella colorans DSM 100333	GCA_003096815	scaffold	GenBank	1703337 tax ID
[Ref.: #66792]	Prevotella colorans strain DSM 100333	1703337.3	wgs	PATRIC	1703337 tax ID	*
[Ref.: #66792]	Prevotella colorans DSM 100333	2756170230	draft	JGI IMG/M	1703337 tax ID	*

[Ref.: #24775]	GC-content	43.2 mol%	high performance liquid chromatography (HPLC)
[Ref.: #67771]	GC-content	43.2 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Prevotella colorans DSM 100333 NCBI: GCA_003096815.1
[Ref.: #69481]	spore-forming	no	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Prevotella colorans strain DSM 100333 PATRIC: 1703337.3
[Ref.: #69480]	motile	no	92.494	yes
[Ref.: #69480]	gram-positive	no	97.4	yes
[Ref.: #69480]	anaerobic	yes	99.137	yes
[Ref.: #69480]	aerobic	no	98.55	yes
[Ref.: #69480]	halophile	no	87.739	no
[Ref.: #69480]	spore-forming	no	97.643	yes
[Ref.: #69480]	thermophile	no	94.16	no
[Ref.: #69480]	glucose-util	yes	85.77	no
[Ref.: #69480]	flagellated	no	97.269	no
[Ref.: #69480]	glucose-ferment	yes	60.04	no

Availability in culture collections External links

[Ref.: #24775]

Culture collection no.

DSM 100333, CCOS 902, CCUG 67421, KCTC 15694

[Ref.: #91263]

SI-ID 397050

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Prevotella colorans sp. nov., isolated from a human wound.	Buhl M, Willmann M, Liese J, Autenrieth IB, Marschal M	Int J Syst Evol Microbiol	10.1099/ijsem.0.001134	2016	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#24775

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 100333

#43096

Michael Buhl, Matthias Willmann, Jan Liese, Ingo B. Autenrieth, Matthias Marschal: Prevotella colorans sp. nov., isolated from a human wound. IJSEM 66: 3005 - 3009 2016 ( DOI 10.1099/ijsem.0.001134 , PubMed 27150727 )

#63417 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 67421

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67771 Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;

#68380

Automatically annotated from API rID32A .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#91263

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID397050.1 )

* These data were automatically processed and therefore are not curated

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Prevotella colorans DSM 100333

Prevotella colorans strain DSM 100333

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