Mycoplana azooxidifex Po 20/26 | Type strain | DSM 100211, LMG 28788

Strain identifier

BacDive ID: 132978

Type strain:

Species: Mycoplana azooxidifex

Strain Designation: Po 20/26

Strain history: <- U. Behrendt, Leibniz-Centre Agricultural Landscape Res. (ZALF), Müncheberg, Germany; Po 20/26

SI-ID 406247

DSM 100211

Other strains from Mycoplana azooxidifex

Section

External links

Mycoplana azooxidifex Po 20/26 is a facultative anaerobe, mesophilic, Gram-negative bacterium that forms circular colonies and was isolated from soil cores from a field experiment "CarboZALF - Carbon dynamics on arable sites".

colony-forming
Gram-negative
rod-shaped
facultative anaerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Alphaproteobacteria*
	Order	*Hyphomicrobiales*
	Family	*Rhizobiaceae*
	Genus	*Mycoplana*
	Species	**Mycoplana azooxidifex**
	Full Scientific Name (LPSN)	Mycoplana azooxidifex (Behrendt et al. 2016) Kuzmanovi? et al. 2022

Synonym

Rhizobium azooxidifex

Information on morphological and physiological properties Morphology

[Ref.: #43980]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %99.987

[Ref.: #43980]	Cell length	2.2-2.4 µm

[Ref.: #43980]	Cell width	0.8-1.0 µm

[Ref.: #43980]	Cell shape	rod-shaped

[Ref.: #43980]	Motility	no

[Ref.: #43980]	Colony size	0.5-1.0 mm
[Ref.: #43980]	Colony color	beige-brownish
[Ref.: #43980]	Colony shape	circular
[Ref.: #43980]	Incubation period	3 days
[Ref.: #43980]	Cultivation medium used	Nutrient agar

Information on culture and growth conditions Culture and growth conditions

[Ref.: #43980]

Culture medium

Nutrient agar (NA)

[Ref.: #43980]

Culture medium growth

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Culture medium

Tryptone soy agar

[Ref.: #43980]

Culture medium growth

[Ref.: #24766]

Culture medium

RHIZOBIUM MEDIUM (DSMZ Medium 98)

[Ref.: #24766]

Culture medium growth

[Ref.: #24766]

Culture medium link

Medium recipe at MediaDive

[Ref.: #24766]

Culture medium composition

expand / minimize

[Ref.: #43980]

Culture medium

MacConkey agar

[Ref.: #43980]

Culture medium growth

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Culture medium

Reasoner's 2A agar (R2A)

[Ref.: #43980]

Culture medium growth

	Kind of temperature		Temperature
[Ref.: #24766]		growth	30 °C
[Ref.: #43980]		optimum	25-30 °C
[Ref.: #43980]	no	growth	10 °C
[Ref.: #43980]	no	growth	37 °C

[Ref.: #24766]	Temperature range	mesophilic
[Ref.: #43980]	Temperature range	mesophilic
[Ref.: #43980]	Temperature range	psychrophilic
[Ref.: #43980]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #43980]			optimum	8
[Ref.: #43980]			growth	6.0-8.0

Information on physiology and metabolism Physiology and metabolism

[Ref.: #43980]

Oxygen tolerance

facultative anaerobe

[Ref.: #43980]	Ability of spore formation	no
[Ref.: #69481]	Ability of spore formation	no Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.998

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #43980]		NaCl		growth	0-3	%(w/v)
[Ref.: #43980]		NaCl		optimum	1	%(w/v)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #43980]	2,3-butanediol ChEBI	-	energy source
[Ref.: #43980]	2-hydroxybutyrate ChEBI	-	energy source
[Ref.: #43980]	2-oxobutanoate ChEBI	-	energy source
[Ref.: #43980]	2-oxoglutarate ChEBI	+	energy source
[Ref.: #43980]	2-oxopentanoate ChEBI	-	energy source
[Ref.: #43980]	4-hydroxy-L-proline ChEBI	-	energy source
[Ref.: #43980]	4-hydroxybutyrate ChEBI	-	energy source
[Ref.: #43980]	4-hydroxyphenylacetic acid ChEBI	-	energy source
[Ref.: #43980]	acetate ChEBI	-	energy source
[Ref.: #68369]	adipate ChEBI	-	assimilation	* from API 20NE
[Ref.: #43980]	alpha-cyclodextrin ChEBI	-	energy source
[Ref.: #43980]	alpha-lactose ChEBI	-	energy source
[Ref.: #68369]	arginine ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #43980]	bromosuccinate ChEBI	+	energy source
[Ref.: #43980]	casein	-	hydrolysis
[Ref.: #43980]	cellulose ChEBI	-	hydrolysis
[Ref.: #43980]	cis-aconitate ChEBI	-	energy source
[Ref.: #43980]	citrate ChEBI	-	energy source
[Ref.: #43980]	D-alanine ChEBI	-	energy source
[Ref.: #43980]	D-arabitol ChEBI	+	energy source
[Ref.: #43980]	D-fructose ChEBI	+	energy source
[Ref.: #43980]	D-galactonic acid lactone ChEBI	-	energy source
[Ref.: #43980]	D-galactose ChEBI	+	energy source
[Ref.: #43980]	D-galacturonic acid ChEBI	-	energy source
[Ref.: #43980]	D-gluconate ChEBI	-	energy source
[Ref.: #43980]	D-glucosaminic acid ChEBI	-	energy source
[Ref.: #68369]	D-glucose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	-	fermentation	* from API 20NE
[Ref.: #43980]	D-glucose 6-phosphate ChEBI	-	energy source
[Ref.: #43980]	D-glucuronate ChEBI	-	energy source
[Ref.: #43980]	D-mannitol ChEBI	+	energy source
[Ref.: #68369]	D-mannitol ChEBI	+	assimilation	* from API 20NE
[Ref.: #43980]	D-mannose ChEBI	+	energy source
[Ref.: #68369]	D-mannose ChEBI	+	assimilation	* from API 20NE
[Ref.: #43980]	D-saccharate ChEBI	-	energy source
[Ref.: #43980]	D-serine ChEBI	-	energy source
[Ref.: #43980]	D-sorbitol ChEBI	+	energy source
[Ref.: #68369]	decanoate ChEBI	-	assimilation	* from API 20NE
[Ref.: #43980]	erythritol ChEBI	+	energy source
[Ref.: #43980]	esculin ChEBI	+	hydrolysis
[Ref.: #68369]	esculin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #43980]	formate ChEBI	-	energy source
[Ref.: #43980]	gamma-aminobutyric acid ChEBI	-	energy source
[Ref.: #68369]	gelatin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #43980]	gentiobiose ChEBI	+	energy source
[Ref.: #68369]	gluconate ChEBI	-	assimilation	* from API 20NE
[Ref.: #43980]	glucose ChEBI	+	energy source
[Ref.: #43980]	glucose 1-phosphate ChEBI	-	energy source
[Ref.: #43980]	glycerol ChEBI	+	energy source
[Ref.: #43980]	glycerol 1-phosphate ChEBI	-	energy source
[Ref.: #43980]	glycogen ChEBI	-	energy source
[Ref.: #43980]	glycyl L-aspartic acid ChEBI	-	energy source
[Ref.: #43980]	glycyl-l-glutamate ChEBI	-	energy source
[Ref.: #43980]	inosine ChEBI	-	energy source
[Ref.: #43980]	itaconate ChEBI	-	energy source
[Ref.: #43980]	L-alaninamide ChEBI	-	energy source
[Ref.: #43980]	L-alanine 4-nitroanilide	-	energy source
[Ref.: #43980]	L-alanylglycine ChEBI	-	energy source
[Ref.: #43980]	L-arabinose ChEBI	+	energy source
[Ref.: #68369]	L-arabinose ChEBI	+	assimilation	* from API 20NE
[Ref.: #43980]	L-asparagine ChEBI	-	energy source
[Ref.: #43980]	L-aspartate ChEBI	-	energy source
[Ref.: #43980]	L-fucose ChEBI	-	energy source
[Ref.: #43980]	L-glutamate ChEBI	+	energy source
[Ref.: #43980]	L-histidine ChEBI	-	energy source
[Ref.: #43980]	L-leucine ChEBI	-	energy source
[Ref.: #43980]	L-ornithine ChEBI	-	energy source
[Ref.: #43980]	L-phenylalanine ChEBI	-	energy source
[Ref.: #43980]	L-proline ChEBI	-	energy source
[Ref.: #43980]	L-pyroglutamic acid ChEBI	-	energy source
[Ref.: #43980]	L-rhamnose ChEBI	+	energy source
[Ref.: #43980]	L-serine ChEBI	-	energy source
[Ref.: #43980]	L-threonine ChEBI	-	energy source
[Ref.: #43980]	lactate ChEBI	+	energy source
[Ref.: #43980]	lactulose ChEBI	-	energy source
[Ref.: #68369]	malate ChEBI	+	assimilation	* from API 20NE
[Ref.: #43980]	malonate ChEBI	-	energy source
[Ref.: #68369]	maltose ChEBI	+	assimilation	* from API 20NE
[Ref.: #43980]	maltose hydrate	+	energy source
[Ref.: #43980]	melibiose ChEBI	-	energy source
[Ref.: #43980]	methyl beta-D-glucopyranoside ChEBI	-	energy source
[Ref.: #43980]	methyl pyruvate ChEBI	+	energy source
[Ref.: #43980]	monomethyl succinate ChEBI	+	energy source
[Ref.: #43980]	myo-inositol ChEBI	+	energy source
[Ref.: #43980]	N-acetylgalactosamine ChEBI	-	energy source
[Ref.: #43980]	N-acetylglucosamine ChEBI	+	energy source
[Ref.: #68369]	N-acetylglucosamine ChEBI	+	assimilation	* from API 20NE
[Ref.: #43980]	nitrate ChEBI	+	reduction
[Ref.: #68369]	nitrate ChEBI	-	reduction	* from API 20NE
[Ref.: #43980]	nitrite ChEBI	+	reduction
[Ref.: #43980]	phenylethylamine ChEBI	-	energy source
[Ref.: #43980]	propionate ChEBI	-	energy source
[Ref.: #43980]	putrescine ChEBI	-	energy source
[Ref.: #43980]	quinate ChEBI	-	energy source
[Ref.: #43980]	raffinose ChEBI	-	energy source
[Ref.: #43980]	ribitol ChEBI	+	energy source
[Ref.: #43980]	sebacic acid ChEBI	-	energy source
[Ref.: #43980]	starch ChEBI	-	hydrolysis
[Ref.: #43980]	succinamate ChEBI	+	energy source
[Ref.: #43980]	succinate ChEBI	+	energy source
[Ref.: #43980]	sucrose ChEBI	+	energy source
[Ref.: #43980]	thymidine ChEBI	-	energy source
[Ref.: #43980]	trehalose ChEBI	+	energy source
[Ref.: #68369]	tryptophan ChEBI	-	energy source	* from API 20NE
[Ref.: #43980]	turanose ChEBI	+	energy source
[Ref.: #43980]	tween 40 ChEBI	-	energy source
[Ref.: #43980]	tween 80 ChEBI	-	energy source
[Ref.: #43980]	uridine ChEBI	-	energy source
[Ref.: #43980]	urocanic acid ChEBI	-	energy source
[Ref.: #43980]	xylitol ChEBI	-	energy source
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #43980]		indole ChEBI	no
[Ref.: #68369]		indole ChEBI	no	* from API 20NE

	Physiological tests	Metabolite	Indole test
[Ref.: #68369]		indole ChEBI	-	* from API 20NE

	Enzyme	Enzyme activity	EC number
[Ref.: #43980]	alkaline phosphatase	+	3.1.3.1
[Ref.: #43980]	alpha-glucosidase	+	3.2.1.20
[Ref.: #43980]	arginine dihydrolase	-	3.5.3.6
[Ref.: #68369]	arginine dihydrolase	-	3.5.3.6	* from API 20NE
[Ref.: #43980]	beta-galactosidase	-	3.2.1.23
[Ref.: #68369]	beta-glucosidase	-	3.2.1.21	* from API 20NE
[Ref.: #43980]	beta-glucuronidase	-	3.2.1.31
[Ref.: #43980]	catalase	+	1.11.1.6
[Ref.: #43980]	cytochrome oxidase	+	1.9.3.1
[Ref.: #68369]	cytochrome oxidase	+	1.9.3.1	* from API 20NE
[Ref.: #43980]	DNase	-
[Ref.: #43980]	gelatinase	-
[Ref.: #68369]	gelatinase	-		* from API 20NE
[Ref.: #43980]	N-acetyl-beta-glucosaminidase	-	3.2.1.52
[Ref.: #43980]	pyrazinamidase	+	3.5.1.B15
[Ref.: #43980]	urease	-	3.5.1.5
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #43980]

Fatty acid	Percentage
C18:1ω7c	69.6
C17:0 iso	15.3
C19:0ω8c cyclo	13.6
C14:0 3-OH / C16:1 iso I	7.1
C16:0	3.4
C16:1ω7c / C15:1 iso-2OH	1.9
10-methyl C19:0	1.9
C18:0 3OH	1.5
C16:0 3OH	1

API® 20NE:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitratesNO3	Indole productionTRP	Fermentation of D-glucoseGLU_ Ferm	Arginine dihydrolaseADH (Arg)	Urease/urea hydrolysisURE	Esculin hydrolysis/beta-GlucosidaseESC	Gelatinase/gelatin hydrolysisGEL	beta-GalactosidasePNPG	Assimilation of D-glucoseGLU_ Assim	Assimilation of L-arabinoseARA	Assimilation of D-mannoseMNE	Assimilation of D-mannitolMAN	Assimilation of N-acetyl-glucosamineNAG	Assimilation of maltoseMAL	Assimilation of gluconateGNT	Assimilation of capric acidCAP	Assimilation adipic acidADI	Assimilation of malic acidMLT	Assimilation of citric acidCIT	Assimilation of phenyl-acetic acidPAC	Cytochrome oxidaseOX
[Ref.: #24766]	24947	-	-	-	-	+	-	-	-	+	+	+	+	+	+	-	-	-	+	-	-	not determinedn.d.
[Ref.: #24766]	24570	-	-	-	-	-	-	-	-	+	+	+	+	+	+	-	-	-	+	-	-	+
[Ref.: #24766]	24202	-	-	-	-	-	-	-	-	+	+	+	+	+	+	-	-	-	+	-	-	not determinedn.d.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #24766]	Sample type/isolated from	soil cores from a field experiment "CarboZALF - Carbon dynamics on arable sites"
[Ref.: #24766]	Geographic location (country and/or sea, region)	State Brandenburg, Uckermark, Dedelow (53° 22' N 13° 47' E)
[Ref.: #24766]	Country	Germany
[Ref.: #24766]	Country ISO 3 Code	DEU
[Ref.: #24766]	Continent	Europe
[Ref.: #24766]	Geographic location	53.3667°/13.7833°

[Ref.: #43980]	Sample type/isolated from	soil cores, Uckermark, Brandenburg, Germany
[Ref.: #43980]	Geographic location (country and/or sea, region)	Uckermark, Brandeburg
[Ref.: #43980]	Country	Germany
[Ref.: #43980]	Country ISO 3 Code	DEU
[Ref.: #43980]	Continent	Europe
[Ref.: #43980]	Geographic location	53.3667°/13.7833°
* marker position based on {}

Isolation sources categories

#Engineered	#Agriculture	#Field
#Environmental	#Terrestrial	#Core sample
#Environmental	#Terrestrial	#Soil

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence LN832063 (>99% sequence identity) for Rhizobium azooxidifex from Microbeatlas

Aquatic Samples	2928
Soil Samples	7114
Animal Samples	1079
Plant Samples	3715
Total Samples	14836

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #24766]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #24766]	Rhizobium sp. Po 20/26 partial 16S rRNA gene, strain Po 20/26	LN832063	1390	ENA	1636188 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Mycoplana azooxidifex DSM 100211	GCA_014196765	scaffold	GenBank	1636188 tax ID	*
[Ref.: #66792]	Rhizobium azooxidifex strain DSM 100211	1636188.3	wgs	PATRIC	1636188 tax ID	*
[Ref.: #66792]	Rhizobium azooxidifex DSM 100211	2829954201	draft	JGI IMG/M	1636188 tax ID	*

[Ref.: #43980]	GC-content	52.8 mol%	Thermal denaturation, fluorometry
[Ref.: #24766]	GC-content	52.8 mol%	fluorimetric

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Rhizobium azooxidifex DSM 100211 NCBI: GCA_014196765.1
[Ref.: #69481]	spore-forming	no	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Rhizobium azooxidifex DSM 100211 NCBI: GCA_014196765.1
[Ref.: #69480]	flagellated	no	82.895	yes
[Ref.: #69480]	gram-positive	no	97.824	yes
[Ref.: #69480]	anaerobic	no	98.745	yes
[Ref.: #69480]	aerobic	yes	88.434	no
[Ref.: #69480]	halophile	no	93.743	yes
[Ref.: #69480]	spore-forming	no	94.521	yes
[Ref.: #69480]	glucose-util	yes	91.205	yes
[Ref.: #69480]	thermophile	no	99.231	yes
[Ref.: #69480]	motile	yes	84.305	no
[Ref.: #69480]	glucose-ferment	no	89.321	yes

Availability in culture collections External links

[Ref.: #24766]

Culture collection no.

DSM 100211, LMG 28788

[Ref.: #91254]

SI-ID 406247

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Rhizobium rhizolycopersici sp. nov., Isolated from the Rhizosphere Soil of Tomato Plants in China.	Thin KK, He SW, Wang X, Wang Y, Rong M, Han JG, Zhang X	Curr Microbiol	10.1007/s00284-020-02323-6	2021	*
Phylogeny	Georhizobium profundi gen. nov., sp. nov., a piezotolerant bacterium isolated from a deep-sea sediment sample of the New Britain Trench.	Cao J, Wei Y, Lai Q, Wu Y, Deng J, Li J, Liu R, Wang L, Fang J	Int J Syst Evol Microbiol	10.1099/ijsem.0.003766	2020	*
Phylogeny	Gellertiella hungarica gen. nov., sp. nov., a novel bacterium of the family Rhizobiaceae isolated from a spa in Budapest.	Toth E, Szuroczki S, Keki Z, Boka K, Szili-Kovacs T, Schumann P	Int J Syst Evol Microbiol	10.1099/ijsem.0.002332	2017	*
Phylogeny	Characterization of the N2O-producing soil bacterium Rhizobium azooxidifex sp. nov.	Behrendt U, Kampfer P, Glaeser SP, Augustin J, Ulrich A	Int J Syst Evol Microbiol	10.1099/ijsem.0.001036	2016	*
Phylogeny	Rhizobium album sp. nov., isolated from a propanil-contaminated soil.	Hang P, Zhang L, Zhou XY, Hu Q, Jiang JD	Antonie Van Leeuwenhoek	10.1007/s10482-018-1160-3	2018	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#24766

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 100211

#43980

Undine Behrendt, Peter Kämpfer, Stefanie P. Glaeser, Jürgen Augustin, Andreas Ulrich: Characterization of the N2O-producing soil bacterium Rhizobium azooxidifex sp. nov.. IJSEM 66: 2354 - 2361 2016 ( DOI 10.1099/ijsem.0.001036 , PubMed 27030972 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68369

Automatically annotated from API 20NE .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#91254

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID406247.1 )

* These data were automatically processed and therefore are not curated

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Rhizobium azooxidifex DSM 100211

Rhizobium azooxidifex DSM 100211

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Change proposal