Name: Spirosoma aerophilum 5516J-17
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 132974

Type strain:

Species: Spirosoma aerophilum

Strain Designation: 5516J-17

Culture col. no.: DSM 28388, JCM 19950, KACC 17323

Strain history: S.-W. Kwon 5516J-17.

NCBI tax ID(s): 1717229 (species)

SI-ID 398594

DSM 28388

Section

Spirosoma aerophilum 5516J-17 is an aerobe, Gram-negative, rod-shaped bacterium that forms irregular colonies and was isolated from air sample.

colony-forming
Gram-negative
rod-shaped
aerobe
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacteroidota*
	Class	*Cytophagia*
	Order	*Cytophagales*
	Family	*Spirosomataceae*
	Genus	*Spirosoma*
	Species	**Spirosoma aerophilum**
	Full Scientific Name (LPSN)	Spirosoma aerophilum Kim et al. 2016

Information on morphological and physiological properties Morphology

[Ref.: #43963]	Gram stain	negative

[Ref.: #43963]	Cell length	2.5-3.5 µm

[Ref.: #43963]	Cell width	1 µm

[Ref.: #43963]	Cell shape	rod-shaped

[Ref.: #43963]	Motility	no

[Ref.: #43963]	Colony color	yellow
[Ref.: #43963]	Colony shape	irregular
[Ref.: #43963]	Incubation period	2 days
[Ref.: #43963]	Cultivation medium used	R2A agar

Information on culture and growth conditions Culture and growth conditions

[Ref.: #43963]

Culture medium

Reasoner's 2A agar (R2A)

[Ref.: #43963]

Culture medium growth

[Ref.: #24762]

Culture medium

R2A MEDIUM (DSMZ Medium 830)

[Ref.: #24762]

Culture medium growth

[Ref.: #24762]

Culture medium link

Medium recipe at MediaDive

[Ref.: #24762]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #24762]	growth	28 °C
[Ref.: #43963]	growth	10-37 °C
[Ref.: #43963]	optimum	28-30 °C
[Ref.: #67770]	growth	28 °C

[Ref.: #24762]	Temperature range	mesophilic
[Ref.: #43963]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #43963]			optimum	7
[Ref.: #43963]			growth	6.0-11.0

Information on physiology and metabolism Physiology and metabolism

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Oxygen tolerance

aerobe

[Ref.: #43963]

Ability of spore formation

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #43963]		NaCl		growth	0-1	%(w/v)

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Observation

quinon MK-7

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #43963]	3-hydroxybenzoate ChEBI	-	assimilation
[Ref.: #43963]	3-hydroxybutyrate ChEBI	-	assimilation
[Ref.: #43963]	4-hydroxybenzoate ChEBI	-	assimilation
[Ref.: #43963]	adipate ChEBI	-	assimilation
[Ref.: #43963]	carboxymethylcellulose ChEBI	-	hydrolysis
[Ref.: #43963]	casein	-	hydrolysis
[Ref.: #43963]	chitin ChEBI	-	hydrolysis
[Ref.: #43963]	D-glucose ChEBI	+	assimilation
[Ref.: #43963]	D-mannitol ChEBI	-	assimilation
[Ref.: #43963]	D-mannose ChEBI	+	assimilation
[Ref.: #43963]	D-ribose ChEBI	-	assimilation
[Ref.: #43963]	D-sorbitol ChEBI	-	assimilation
[Ref.: #43963]	decanoate ChEBI	-	assimilation
[Ref.: #43963]	dna ChEBI	-	hydrolysis
[Ref.: #43963]	esculin ChEBI	+	hydrolysis
[Ref.: #43963]	gelatin ChEBI	-	hydrolysis
[Ref.: #43963]	glucose ChEBI	-	fermentation
[Ref.: #43963]	glycogen ChEBI	+	assimilation
[Ref.: #43963]	hypoxanthine ChEBI	-	hydrolysis
[Ref.: #43963]	itaconate ChEBI	-	assimilation
[Ref.: #43963]	L-alanine ChEBI	-	assimilation
[Ref.: #43963]	L-arabinose ChEBI	+	assimilation
[Ref.: #43963]	L-fucose ChEBI	-	assimilation
[Ref.: #43963]	L-histidine ChEBI	-	assimilation
[Ref.: #43963]	L-proline ChEBI	-	assimilation
[Ref.: #43963]	L-rhamnose ChEBI	+	assimilation
[Ref.: #43963]	L-serine ChEBI	-	assimilation
[Ref.: #43963]	lactate ChEBI	-	assimilation
[Ref.: #43963]	malate ChEBI	-	assimilation
[Ref.: #43963]	maltose ChEBI	+	assimilation
[Ref.: #43963]	melibiose ChEBI	+	assimilation
[Ref.: #43963]	myo-inositol ChEBI	-	assimilation
[Ref.: #43963]	N-acetylglucosamine ChEBI	+	assimilation
[Ref.: #43963]	nitrate ChEBI	-	reduction
[Ref.: #43963]	phenylacetate ChEBI	-	assimilation
[Ref.: #43963]	potassium 2-dehydro-D-gluconate	-	assimilation
[Ref.: #43963]	potassium 5-dehydro-D-gluconate	-	assimilation
[Ref.: #43963]	propionate ChEBI	-	assimilation
[Ref.: #43963]	salicin ChEBI	+	assimilation
[Ref.: #43963]	sodium acetate ChEBI	-	assimilation
[Ref.: #43963]	sodium citrate ChEBI	-	assimilation
[Ref.: #43963]	sodium malonate ChEBI	-	assimilation
[Ref.: #43963]	starch ChEBI	-	hydrolysis
[Ref.: #43963]	suberic acid ChEBI	-	assimilation
[Ref.: #43963]	sucrose ChEBI	+	assimilation
[Ref.: #43963]	tween 80 ChEBI	+	hydrolysis
[Ref.: #43963]	tyrosine ChEBI	-	hydrolysis
[Ref.: #43963]	urea ChEBI	-	hydrolysis
[Ref.: #43963]	valerate ChEBI	-	assimilation
[Ref.: #43963]	xanthine ChEBI	-	hydrolysis
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #43963]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #43963]	acid phosphatase	+	3.1.3.2
[Ref.: #43963]	alkaline phosphatase	+	3.1.3.1
[Ref.: #43963]	alpha-chymotrypsin	-	3.4.21.1
[Ref.: #43963]	alpha-fucosidase	-	3.2.1.51
[Ref.: #43963]	alpha-galactosidase	+	3.2.1.22
[Ref.: #43963]	alpha-glucosidase	+	3.2.1.20
[Ref.: #43963]	alpha-mannosidase	+	3.2.1.24
[Ref.: #43963]	arginine dihydrolase	-	3.5.3.6
[Ref.: #43963]	beta-galactosidase	+	3.2.1.23
[Ref.: #43963]	beta-glucosidase	-	3.2.1.21
[Ref.: #43963]	beta-glucuronidase	-	3.2.1.31
[Ref.: #43963]	catalase	+	1.11.1.6
[Ref.: #43963]	cystine arylamidase	-	3.4.11.3
[Ref.: #43963]	cytochrome oxidase	-	1.9.3.1
[Ref.: #43963]	esterase (C 4)	+
[Ref.: #43963]	esterase Lipase (C 8)	+
[Ref.: #43963]	leucine arylamidase	+	3.4.11.1
[Ref.: #43963]	lipase (C 14)	-
[Ref.: #43963]	N-acetyl-beta-glucosaminidase	+	3.2.1.52
[Ref.: #43963]	naphthol-AS-BI-phosphohydrolase	+
[Ref.: #43963]	trypsin	-	3.4.21.4
[Ref.: #43963]	valine arylamidase	+
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #43963]

Fatty acid	Percentage
C16:1ω7c / C16:1ω6c	33.9
C16:1ω5c	18.4
C17:0 iso 3OH	8.6
C15:0 iso	8.4
C16:0	7.1
C15:0 anteiso	3.8
C18:0	3.1
C15:0 iso 3OH	2.8
C18:1ω9c	2
C16:0 3OH	1.7
C18:1ω7c / C18:1ω6c	1.6
C14:0	1.5
C17:1 iso ω9c / C16:0 10-methyl	1.4
C18:2ω6,9c / anteiso-C18:0	1.2
iso-C17:1 I / anteiso C17:1 B	1.1
C17:0 2OH	1

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #24762]	Sample type/isolated from	air sample
[Ref.: #24762]	Geographic location (country and/or sea, region)	Jeju Island
[Ref.: #24762]	Country	Republic of Korea
[Ref.: #24762]	Country ISO 3 Code	KOR
[Ref.: #24762]	Continent	Asia

[Ref.: #43963]	Sample type/isolated from	air sample collected from Jeju Island, Republic of Korea
[Ref.: #43963]	Geographic location (country and/or sea, region)	Jeju Island
[Ref.: #43963]	Country	Republic of Korea
[Ref.: #43963]	Country ISO 3 Code	KOR
[Ref.: #43963]	Continent	Asia
[Ref.: #43963]	Enrichment culture	R2A agar
[Ref.: #43963]	Enrichment culture composition	supplemented with 200 µg/mL cycloheximide
[Ref.: #43963]	Enrichment culture duration	7 days
[Ref.: #43963]	Enrichment culture temperature	28 ̊C

[Ref.: #67770]	Sample type/isolated from	Air from Jeju Island
[Ref.: #67770]	Country	Republic of Korea
[Ref.: #67770]	Country ISO 3 Code	KOR
[Ref.: #67770]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Environmental	#Air	-
#Environmental	#Air	#Outdoor Air

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #24762]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #24762]	Spirosoma aerophilum strain 5516J-17 16S ribosomal RNA gene, partial sequence	KR024033	1445	ENA	1717229 tax ID

[Ref.: #43963]	GC-content	55.7 mol%	real time PCR (RTD-PCR)
[Ref.: #24762]	GC-content	55.7 mol%	fluorimetric
[Ref.: #67770]	GC-content	55.7 mol%	thermal denaturation, midpoint method (Tm)

Availability in culture collections External links

[Ref.: #24762]

Culture collection no.

DSM 28388, JCM 19950, KACC 17323

[Ref.: #91250]

SI-ID 398594

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Spirosoma migulaei sp. nov., isolated from soil.	Okiria J, Ten LN, Park SJ, Lee SY, Lee DH, Kang IK, Lee DS, Jung HY	J Microbiol	10.1007/s12275-017-7377-4	2017	*
Phylogeny	Spirosoma aerophilum sp. nov., isolated from an air sample.	Kim SJ, Ahn JH, Weon HY, Hong SB, Seok SJ, Kim JS, Kwon SW	Int J Syst Evol Microbiol	10.1099/ijsem.0.001037	2016	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#24762

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 28388

#43963

Soo-Jin Kim, Jae-Hyung Ahn, Hang-Yeon Weon, Seung-Beom Hong, Soon-Ja Seok, Jeong-Seon Kim, Soon-Wo Kwon: Spirosoma aerophilum sp. nov., isolated from an air sample. IJSEM 66: 2342 - 2346 2016 ( DOI 10.1099/ijsem.0.001037 , PubMed 27031168 )

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#91250

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID398594.1 )

* These data were automatically processed and therefore are not curated

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