Kutzneria viridogrisea (DSM 43850, ATCC 25242, NIHJ 523)

Name: Kutzneria viridogrisea (DSM 43850, ATCC 25242, NIHJ 523)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 13294

Type strain

Strain Designation MCRL 0044, TA 597

Culture col. no. DSM 43850 ATCC 25242 NIHJ 523 MCRL 0044 TA 597 18 more

NCBI tax ID(s) 47990

Special Collections DSMZ JCM KCTC Wink compendium JMRC

History DSMZ <- ATCC <- T. Okuda, MCRL, 0044 JCM <-- KCC A-0282 <-- ATCC 25242 <-- Tanabe Seiyaku Co., Ltd., Japan; MCRL 0044 (TA 597).

Links

Kutzneria viridogrisea MCRL 0044 is a spore-forming bacterium that builds an aerial mycelium and was isolated from soil.

spore-forming genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 38436

ATCC 25242,DSM 43850,JCM 3282,CCRC 13303,HUT 6593,IFO 15561,VKM Ac-1297,CECT 3305,KCTC 9436,AS 4.1348,KCTC 9661,NRRL B-24059,NBRC 15561,BCRC 13303,CGMCC 4.1348

@ref 20215

Last LPSN update 2026-05-29 08:28:00

Domain Bacteria

Phylum Actinomycetota

Class Actinomycetes

Order Pseudonocardiales

Family Pseudonocardiaceae

Genus Kutzneria

Species Kutzneria viridogrisea

Full scientific name Kutzneria viridogrisea (Okuda et al. 1966) Stackebrandt et al. 1994

Synonyms (1)

Streptosporangium viridogriseum

Morphology

Cell morphology

@ref	Gram stain	Confidence	Motility
125439	positive	99.373
125439		94.924

Colony morphology

@ref	Colony color	Incubation period	Medium used
19605	Beige	10-14 days	ISP 2
19605	Beige	10-14 days	ISP 3
19605	Beige	10-14 days	ISP 4
19605	Beige	10-14 days	ISP 5
19605		10-14 days	ISP 6
19605	Beige	10-14 days	ISP 7

Multicellular morphology

@ref	Complex name	Complex color	Medium name
19605	Aerial Mycelium	White	ISP 2
19605			ISP 3
19605			ISP 4
19605	Aerial Mycelium	White	ISP 5
19605			ISP 6
19605	Aerial Mycelium	White	ISP 7

Multimedia

@ref:	11332
multimedia content:	DSM_43850.jpg
multimedia.multimedia content:	https://www.dsmz.de/microorganisms/photos/DSM_43850.jpg
caption:	Medium 65 28°C
intellectual property rights:	© Leibniz-Institut DSMZ
manual_annotation:	1

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
11332	ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84)	Medium recipe at MediaDive	Name: ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84) Composition: Agar 20.0 g/l Rolled oats 20.0 g/l ZnSO4 x 7 H2O 0.001 g/l MnCl2 x 4 H2O 0.001 g/l FeSO4 x 7 H2O 0.001 g/l Distilled water
11332	GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)	Medium recipe at MediaDive	Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
19605	ISP 2		Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19605	ISP 3		Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19605	ISP 4		Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19605	ISP 5		Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
19605	ISP 6		Name: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
19605	ISP 7		Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes

Culture temp

@ref	Growth	Type	Temperature (°C)
11332	positive	growth	28
19605	positive	optimum	30
67770	positive	growth	28

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
125439	obligate aerobe	98.424

Spore formation

@ref	Spore description	Type of spore	Spore formation
19605	Formation of sporangia, spore surface smooth	spore

Compound production

@ref	Compound
11332	sporaviridin
20216	Sporaviridin
19605	Sporaviridin
67770	Sporaviridin

Halophily

@ref	Salt	Growth	Tested relation	Concentration
19605	NaCl	positive	maximum	2.5 %

Observation

67770

Observationquinones: MK-9(II,III-H₄)

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
19605	22599 ChEBI	arabinose	-
68368	29016 ChEBI	arginine	+	hydrolysis	from API 20E
19605	62968 ChEBI	cellulose	-
68368	16947 ChEBI	citrate	-	assimilation	from API 20E
19605	28757 ChEBI	fructose	+
68368	5291 ChEBI	gelatin	+	hydrolysis	from API 20E
19605	17234 ChEBI	glucose	+
68368	25094 ChEBI	lysine	+	degradation	from API 20E
19605	29864 ChEBI	mannitol	+
19605	17268 ChEBI	myo-inositol	+
68368	18257 ChEBI	ornithine	+	degradation	from API 20E
19605	16634 ChEBI	raffinose	-
19605	26546 ChEBI	rhamnose	-
19605	17992 ChEBI	sucrose	-
68368	27897 ChEBI	tryptophan	-	energy source	from API 20E
68368	16199 ChEBI	urea	+	hydrolysis	from API 20E
19605	18222 ChEBI	xylose	-

Metabolite production

@ref	Chebi-ID	Metabolite
68368	15688 ChEBI	acetoin	from API 20E
68368	16136 ChEBI	hydrogen sulfide	from API 20E
68368	35581 ChEBI	indole	from API 20E

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test	Indole test
68368	15688 ChEBI	acetoin	+		from API 20E
68368	35581 ChEBI	indole		-	from API 20E

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68382	alpha-chymotrypsin	+	3.4.21.1	from API zym
68382	alpha-fucosidase	+	3.2.1.51	from API zym
68382	alpha-galactosidase	+	3.2.1.22	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68382	alpha-mannosidase	+	3.2.1.24	from API zym
68368	arginine dihydrolase	+	3.5.3.6	from API 20E
68382	beta-galactosidase	+	3.2.1.23	from API zym
68368	beta-galactosidase	-	3.2.1.23	from API 20E
68382	beta-glucosidase	+	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
68382	cystine arylamidase	+	3.4.11.3	from API zym
68382	esterase (C 4)	-		from API zym
68382	esterase lipase (C 8)	+		from API zym
68368	gelatinase	+		from API 20E
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	+		from API zym
68368	lysine decarboxylase	+	4.1.1.18	from API 20E
68382	N-acetyl-beta-glucosaminidase	+	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
68368	ornithine decarboxylase	+	4.1.1.17	from API 20E
68382	trypsin	+	3.4.21.4	from API zym
68368	tryptophan deaminase	-	4.1.99.1	from API 20E
68368	urease	+	3.5.1.5	from API 20E
68382	valine arylamidase	+		from API zym

API 20E

@ref	ONPG	ADH (Arg)	LDC (Lys)	ODC	CIT	H2S productionH2S	URE	TDA (Trp)	IND	Acetoin production (Voges Proskauer test)VP	GEL	GLU	MAN	INO	Sor	RHA	SAC	MEL	AMY	ARA	OX	Nitrite productionNO2	Reduction to N2N2	MotilityMOB	Growth on MacConkey mediumMAC	OF-O	OF-F
19605	-	+	+	+	-	-	+	-	-	+	+	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
19605	not determinedn.d.	+	-	+	+	+	+	+	+	+	+	+	+	+	-	+	+	+	+	+

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Terrestrial	#Soil

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
11332	soil
67770	Soil	Kawaguchi City, Saitama Pref.	Japan	JPN	Asia

Taxonmaps

69479

Global distribution of 16S sequence U58530 (>99% sequence identity) for Kutzneria from Microbeatlas

Aquatic Samples	34
Soil Samples	1307
Animal Samples	80
Plant Samples	348
Total Samples	1769

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
11332	1	Risk group (German classification) According to TRBA 466
19605	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
124043	ASM4676372v1 assembly for Kutzneria viridogrisea DSM 43850	contig	GCA_046763725			47990	75.46
124043	ASM1413872v1 assembly for Kutzneria viridogrisea DSM 43850	contig	GCA_014138725	47990.4	2863105245	47990	74.71
124043	ASM3952106v1 assembly for Kutzneria viridogrisea JCM 3282	scaffold	GCA_039521065			47990	54.37

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
20218	S.viridogriseum gene for 16S ribosomal RNA	X70429	1362	47990
20218	Kutzneria viridogrisea 16S ribosomal RNA gene sequence	U58530	1410	47990
124043	Kutzneria viridogrisea strain JCM 3282 16S ribosomal RNA gene, partial sequence.	MT760436	1371	47990

GC content

@ref	GC-content (mol%)	Method
67770	70.3	high performance liquid chromatography (HPLC)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: ASM4676372v1 assembly for Kutzneria viridogrisea DSM 43850
NCBI: GCA_046763725

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	gram_stain	BacteriaNetⓘ	positive	99.37	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	98.42	no
125439	motility	BacteriaNetⓘ	no	94.92	no
125439	spore_formation	BacteriaNetⓘ	yes	82.11	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Kutzneria viridogrisea strain DSM 43850
PATRIC: 47990.4

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	84.98	no
125438	anaerobic	anaerobicⓘ	no	94.84	no
125438	spore-forming	spore-formingⓘ	yes	88.52	yes
125438	aerobic	aerobicⓘ	yes	87.74	no
125438	thermophilic	thermophileⓘ	no	94.77	yes
125438	flagellated	motile2+ⓘ	no	88.00	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Genetics	Metabologenomics analysis reveals antibiotic crypticity of Kutzneria viridogrisea DSM 43850.	Dong BC, Liu F, Hu GA, Yu WC, Li ZY, Guan YT, Zhang WW, Wang H, Wei B.	J Appl Microbiol	10.1093/jambio/lxaf114	2025
	Comparative genome features and secondary metabolite biosynthetic potential of Kutzneria chonburiensis and other species of the genus Kutzneria.	Chanama M, Prombutara P, Chanama S.	Sci Rep	10.1038/s41598-023-36039-x	2023
Metabolism	Complete genome sequence of producer of the glycopeptide antibiotic Aculeximycin Kutzneria albida DSM 43870T, a representative of minor genus of Pseudonocardiaceae.	Rebets Y, Tokovenko B, Lushchyk I, Ruckert C, Zaburannyi N, Bechthold A, Kalinowski J, Luzhetskyy A.	BMC Genomics	10.1186/1471-2164-15-885	2014
Phylogeny	Kutzneria chonburiensis sp. nov., isolated from soil.	Chanama M, Thongkrachang N, Suriyachadkun C, Chanama S	Int J Syst Evol Microbiol	10.1099/ijsem.0.000552	2015

References

#11332	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 43850
#19605	Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20216	Curators of the JMRC: Jena Microbial Resource Collection (JMRC):
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68368	Automatically annotated from API 20E .
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Kutzneria viridogrisea (DSM 43850, ATCC 25242, NIHJ 523)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Multicellular morphology

Multimedia

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Compound production

Halophily

Observation

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

API 20E

API zym

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_046763725

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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