Name: Vibrio metoecus OP3H
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 132931

Type strain:

Species: Vibrio metoecus

Strain Designation: OP3H

Culture col. no.: DSM 104290, CIP 110643, LMG 27764

Strain history: CIP <- 2013, Y. Boucher, Alberta Univ., Edmonton, Canada: strain 0P3H "Vibrio metecus"

NCBI tax ID(s): 1481663 (species)

SI-ID 390544

LMG 27764, CIP 110643, DSM 104290, CCUG 74468

Section

Vibrio metoecus OP3H is a mesophilic, Gram-negative, motile bacterium that was isolated from water of oysterpond.

Gram-negative
motile
rod-shaped
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	"Vibrionales"
	Family	*Vibrionaceae*
	Genus	*Vibrio*
	Species	**Vibrio metoecus**
	Full Scientific Name (LPSN)	Vibrio metoecus Kirchberger et al. 2014

Information on morphological and physiological properties Morphology

[Ref.: #120925]	Gram stain	negative

[Ref.: #120925]	Cell shape	rod-shaped

[Ref.: #120925]	Motility	yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #24719]

Culture medium

TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)

[Ref.: #24719]

Culture medium growth

[Ref.: #24719]

Culture medium link

Medium recipe at MediaDive

[Ref.: #24719]

Culture medium composition

expand / minimize

[Ref.: #120925]

Culture medium

CIP Medium 72

[Ref.: #120925]

Culture medium growth

[Ref.: #120925]

Culture medium link

Medium recipe provided by DSMZ

	Temperatures	Kind of temperature		Temperature
[Ref.: #24719]			growth	28 °C

[Ref.: #24719]

Temperature range

mesophilic

Information on physiology and metabolism Physiology and metabolism

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68368]	amygdalin ChEBI	-	fermentation	* from API 20E
[Ref.: #68368]	arginine ChEBI	-	hydrolysis	* from API 20E
[Ref.: #68368]	citrate ChEBI	+	assimilation	* from API 20E
[Ref.: #68368]	D-glucose ChEBI	+	fermentation	* from API 20E
[Ref.: #68368]	D-mannitol ChEBI	+	fermentation	* from API 20E
[Ref.: #68368]	gelatin ChEBI	+	hydrolysis	* from API 20E
[Ref.: #68368]	L-arabinose ChEBI	-	fermentation	* from API 20E
[Ref.: #68368]	L-rhamnose ChEBI	-	fermentation	* from API 20E
[Ref.: #68368]	lysine ChEBI	+	degradation	* from API 20E
[Ref.: #68368]	melibiose ChEBI	-	fermentation	* from API 20E
[Ref.: #68368]	myo-inositol ChEBI	-	fermentation	* from API 20E
[Ref.: #68368]	ornithine ChEBI	+	degradation	* from API 20E
[Ref.: #68368]	sorbitol ChEBI	-	fermentation	* from API 20E
[Ref.: #68368]	sucrose ChEBI	+	fermentation	* from API 20E
[Ref.: #68368]	tryptophan ChEBI	+	energy source	* from API 20E
[Ref.: #68368]	urea ChEBI	-	hydrolysis	* from API 20E
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Production
[Ref.: #68368]	acetoin ChEBI	no	* from API 20E
[Ref.: #68368]	hydrogen sulfide ChEBI	no	* from API 20E
[Ref.: #68368]	indole ChEBI	yes	* from API 20E

	Physiological tests	Metabolite	Voges-Proskauer-test	Indole test
[Ref.: #68368]		acetoin ChEBI	-		* from API 20E
[Ref.: #68368]		indole ChEBI		+	* from API 20E

	Enzyme	Enzyme activity	EC number
[Ref.: #68368]	arginine dihydrolase	-	3.5.3.6	* from API 20E
[Ref.: #68368]	beta-galactosidase	+	3.2.1.23	* from API 20E
[Ref.: #68368]	cytochrome oxidase	-	1.9.3.1	* from API 20E
[Ref.: #68368]	gelatinase	+		* from API 20E
[Ref.: #68368]	lysine decarboxylase	+	4.1.1.18	* from API 20E
[Ref.: #68368]	ornithine decarboxylase	+	4.1.1.17	* from API 20E
[Ref.: #68368]	tryptophan deaminase	-	4.1.99.1	* from API 20E
[Ref.: #68368]	urease	-	3.5.1.5	* from API 20E

API® 20E:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	beta-GalactosidaseONPG	Arginine dihydrolaseADH (Arg)	Lysine decarboxylaseLDC (Lys)	Ornithine decarboxylaseODC	Citrate utilizationCIT	H2S productionH2S	Urease/urea hydrolysisURE	Tryptophan deaminaseTDA (Trp)	Indole productionIND	Acetoin production (Voges Proskauer test)VP	Gelatinase/gelatin hydrolysisGEL	Fermentation/oxidation of D-glucoseGLU	Fermentation/oxidation of D-mannitolMAN	Fermentation/oxidation of inositolINO	Fermentation/oxidation of sorbitolSor	Fermentation/oxidation of L-rhamnoseRHA	Fermentation/oxidation of sucroseSAC	Fermentation/oxidation of melibioseMEL	Fermentation/oxidation of amygdalinAMY	Fermentation/oxidation of L-arabinoseARA	Cytochrome oxidaseOX	Nitrite productionNO2	Reduction to N2N2	MotilityMOB	Growth on MacConkey mediumMAC	Oxidative catabolisation of D-glucoseOF-O	Fermentation of D-glucoseOF-F
[Ref.: #24719]	17685	+	-	+	+	+	-	-	-	+	-	+	+	+	-	-	-	+	-	-	-	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #24719]	Sample type/isolated from	water of oysterpond
[Ref.: #24719]	Geographic location (country and/or sea, region)	Massachussetts
[Ref.: #24719]	Country	USA
[Ref.: #24719]	Country ISO 3 Code	USA
[Ref.: #24719]	Continent	North America

[Ref.: #120925]	Sample type/isolated from	Animal, Oyster pond
[Ref.: #120925]	Geographic location (country and/or sea, region)	Falmouth, MA, USA , Massachusetts
[Ref.: #120925]	Country	United States of America
[Ref.: #120925]	Country ISO 3 Code	USA
[Ref.: #120925]	Continent	North America
[Ref.: #120925]	Isolation date	2006-10-02
* marker position based on {}

Isolation sources categories

#Engineered	#Food production	#Aquaculture
#Environmental	#Aquatic	#Pond (small)

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence KJ647312 (>99% sequence identity) for Vibrio cholerae from Microbeatlas

Aquatic Samples	4727
Soil Samples	252
Animal Samples	8651
Plant Samples	275
Total Samples	13905

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #120925]

Biosafety level

Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #24719]	Vibrio metoecus strain OP3H 16S ribosomal RNA gene, partial sequence	KJ647312	1411	ENA	1481663 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Vibrio metoecus OP3H	GCA_000696385	contig	GenBank	1481663 tax ID	*

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Vibrio metoecus OP3H NCBI: GCA_000696385.1
[Ref.: #69480]	motile	yes	91.082	no
[Ref.: #69480]	flagellated	yes	89.728	no
[Ref.: #69480]	gram-positive	no	99.056	no
[Ref.: #69480]	anaerobic	no	96.635	no
[Ref.: #69480]	halophile	no	58.704	no
[Ref.: #69480]	spore-forming	no	95.694	no
[Ref.: #69480]	glucose-util	yes	90.695	no
[Ref.: #69480]	aerobic	yes	82.905	no
[Ref.: #69480]	thermophile	no	98.522	no
[Ref.: #69480]	glucose-ferment	yes	89.85	yes

Availability in culture collections External links

[Ref.: #24719]

Culture collection no.

DSM 104290, CIP 110643, LMG 27764

[Ref.: #91209]

SI-ID 390544

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Vibrio metoecus sp. nov., a close relative of Vibrio cholerae isolated from coastal brackish ponds and clinical specimens.	Kirchberger PC, Turnsek M, Hunt DE, Haley BJ, Colwell RR, Polz MF, Tarr CL, Boucher Y	Int J Syst Evol Microbiol	10.1099/ijs.0.060145-0	2014	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#24719

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 104290

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68368

Automatically annotated from API 20E .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#91209

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID390544.1 )

#120925 Collection of Institut Pasteur ; Curators of the CIP; CIP 110643
* These data were automatically processed and therefore are not curated

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