Name: Enterococcus lemanii PC32
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 132913

Type strain:

Species: Enterococcus lemanii

Strain Designation: PC32

Culture col. no.: DSM 105069, NRRL B-59661, CCM 8482, CCUG 61260

Strain history: <- CCUG; CCUG 61260 <- T. R. Whitehead and M. A. Cotta; PC32

NCBI tax ID(s): 1159752 (species)

SI-ID 390260

CCUG 61260, NRRL B-59661, LMG 28568, CCM 8482, DSM 105069

Other strains from Enterococcus lemanii

E. lemanii CCUG 61261
E. lemanii CCUG 61369

Section

Enterococcus lemanii PC32 is a microaerophile, mesophilic, Gram-positive bacterium that was isolated from swine manure storage pit.

Gram-positive
mesophilic
microaerophile
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Lactobacillales*
	Family	*Enterococcaceae*
	Genus	*Enterococcus*
	Species	**Enterococcus lemanii**
	Full Scientific Name (LPSN)	Enterococcus lemanii Cotta et al. 2013

Information on morphological and physiological properties Morphology

[Ref.: #69480]	Gram stain	positive Confidence in %100

Information on culture and growth conditions Culture and growth conditions

[Ref.: #24701]

Culture medium

COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)

[Ref.: #24701]

Culture medium growth

[Ref.: #24701]

Culture medium link

Medium recipe at MediaDive

[Ref.: #24701]

Culture medium composition

expand / minimize

[Ref.: #24701]

Culture medium

TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)

[Ref.: #24701]

Culture medium growth

[Ref.: #24701]

Culture medium link

Medium recipe at MediaDive

[Ref.: #24701]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #24701]			growth	37 °C
[Ref.: #62596]			growth	37 °C

[Ref.: #24701]	Temperature range	mesophilic
[Ref.: #62596]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #62596]

Oxygen tolerance

microaerophile

[Ref.: #69480]

Ability of spore formation

no Confidence in %99.674

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68381]	alpha-cyclodextrin ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68381]	arginine ChEBI	-	hydrolysis	* from API rID32STR
[Ref.: #68381]	D-arabitol ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68381]	D-mannitol ChEBI	+	builds acid from	* from API rID32STR
[Ref.: #68381]	D-ribose ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68381]	D-tagatose ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68381]	glycogen ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68381]	hippurate ChEBI	-	hydrolysis	* from API rID32STR
[Ref.: #68381]	L-arabinose ChEBI	+	builds acid from	* from API rID32STR
[Ref.: #68381]	lactose ChEBI	+	builds acid from	* from API rID32STR
[Ref.: #68381]	maltose ChEBI	+	builds acid from	* from API rID32STR
[Ref.: #68381]	melezitose ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68381]	melibiose ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68381]	methyl beta-D-glucopyranoside ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68381]	pullulan ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68381]	raffinose ChEBI	+	builds acid from	* from API rID32STR
[Ref.: #68381]	sorbitol ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68381]	sucrose ChEBI	+	builds acid from	* from API rID32STR
[Ref.: #68381]	trehalose ChEBI	+	builds acid from	* from API rID32STR
[Ref.: #68381]	urea ChEBI	-	hydrolysis	* from API rID32STR
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #68381]		acetoin ChEBI	no	* from API rID32STR

	Physiological tests	Metabolite	Voges-Proskauer-test
[Ref.: #68381]		acetoin ChEBI	-	* from API rID32STR

	Enzyme	Enzyme activity	EC number
[Ref.: #68381]	Alanyl-Phenylalanyl-Proline arylamidase	-		* from API rID32STR
[Ref.: #68381]	alkaline phosphatase	-	3.1.3.1	* from API rID32STR
[Ref.: #68381]	alpha-galactosidase	+	3.2.1.22	* from API rID32STR
[Ref.: #68381]	arginine dihydrolase	-	3.5.3.6	* from API rID32STR
[Ref.: #68381]	beta-galactosidase	+	3.2.1.23	* from API rID32STR
[Ref.: #68381]	beta-glucosidase	-	3.2.1.21	* from API rID32STR
[Ref.: #68381]	beta-glucuronidase	-	3.2.1.31	* from API rID32STR
[Ref.: #68381]	beta-mannosidase	-	3.2.1.25	* from API rID32STR
[Ref.: #68381]	glycyl tryptophan arylamidase	-		* from API rID32STR
[Ref.: #68381]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API rID32STR
[Ref.: #68381]	pyrrolidonyl arylamidase	+	3.4.19.3	* from API rID32STR
[Ref.: #68381]	urease	-	3.5.1.5	* from API rID32STR
	Only first 10 entries are displayed. Click here to see all.

API® rID32STR:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Arginine dihydrolaseADH (Arg)	beta-Glucosidasebeta GLU	beta-Galactosidasebeta GAR	beta-Glucuronidasebeta GUR	alpha-Galactosidasealpha GAL	Alkaline PhosphatasePAL	Acid from D-riboseRIB	Acid from D-mannitolMAN	Acid from sorbitolSOR	Acid from lactoseLAC	Acid from trehaloseTRE	Acid from raffinoseRAF	Acid from sucroseSAC	Acid from L-arabinoseLARA	Acid from D-arabitolDARL	Acid from alpha-cyclodextrinCDEX	Acetoin production (Voges Proskauer test)VP	Alanyl-Phenylalanyl-Proline arylamidaseAPPA	beta-Galactosidasebeta GAL	Pyrrolidonyl arylamidasePyrA	N-Acetyl-glucosaminidasebeta NAG	Glycyl-tryptophan arylamidaseGTA	Hydrolysis of hippuric acidHIP	Acid from glycogenGLYG	Acid from pullulanPUL	Acid from maltoseMAL	Acid from melibioseMEL	Acid from melezitoseMLZ	Acidification of methyl beta-D-glucopyranosideMbeta DG	Acid from D-tagatoseTAG	beta-Mannosidasebeta MAN	Urease/urea hydrolysisURE
[Ref.: #24701]	10741	-	-	+	-	+	-	-	+	-	+	+	+	+	+	-	-	-	-	+	+	-	-	-	-	-	+	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #24701]	Sample type/isolated from	swine manure storage pit
[Ref.: #24701]	Geographic location (country and/or sea, region)	Eureka near Peoria, Illinois
[Ref.: #24701]	Country	USA
[Ref.: #24701]	Country ISO 3 Code	USA
[Ref.: #24701]	Continent	North America

[Ref.: #62596]	Sample type/isolated from	Swine manure stored in a deep pit
[Ref.: #62596]	Sampling date	1998-07-01
[Ref.: #62596]	Geographic location (country and/or sea, region)	Eureka,Illinois
[Ref.: #62596]	Country	USA
[Ref.: #62596]	Country ISO 3 Code	USA
[Ref.: #62596]	Continent	North America
* marker position based on {}

Isolation sources categories

#Engineered	#Built environment	#Container (Reservoir)
#Host	#Mammals	#Suidae (Pig,Swine)
#Host Body Product	#Gastrointestinal tract	#Feces (Stool)

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AF445301 (>99% sequence identity) for Enterococcus from Microbeatlas

Aquatic Samples	82
Soil Samples	9
Animal Samples	219
Plant Samples	19
Total Samples	329

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #24701]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #24701]	Enterococcus lemanii strain PC32 16S ribosomal RNA gene, partial sequence	AF445301	1551	ENA	1159752 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Enterococcus lemanii DSM 105069	GCA_016909125	contig	GenBank	1159752 tax ID	*
[Ref.: #66792]	Enterococcus lemanii strain DSM 105069	1159752.3	wgs	PATRIC	1159752 tax ID	*
[Ref.: #66792]	Enterococcus lemanii DSM 105069	2901272077	draft	JGI IMG/M	1159752 tax ID	*

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Enterococcus lemanii strain DSM 105069 PATRIC: 1159752.3
[Ref.: #69481]	spore-forming	no	58	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Enterococcus lemanii strain DSM 105069 PATRIC: 1159752.3
[Ref.: #69480]	motile	yes	52.632	no
[Ref.: #69480]	gram-positive	yes	89.825	no
[Ref.: #69480]	anaerobic	no	94.201	yes
[Ref.: #69480]	aerobic	no	96.566	yes
[Ref.: #69480]	halophile	yes	59.636	no
[Ref.: #69480]	spore-forming	no	64.079	no
[Ref.: #69480]	flagellated	no	79.883	no
[Ref.: #69480]	thermophile	no	96.632	no
[Ref.: #69480]	glucose-util	yes	88.718	no
[Ref.: #69480]	glucose-ferment	yes	83.968	no

Availability in culture collections External links

[Ref.: #24701]

Culture collection no.

DSM 105069, NRRL B-59661, CCM 8482, CCUG 61260

[Ref.: #91191]

SI-ID 390260

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Erratum to: Two novel species Enterococcus lemanii sp. nov. and Enterococcus eurekensis sp. nov., isolated from a swine-manure storage pit.	Cotta MA, Whitehead TR, Falsen E, Moore E, Lawson PA	Antonie Van Leeuwenhoek	10.1007/s10482-013-9908-2	2013	*
Phylogeny	Two novel species Enterococcus lemanii sp. nov. and Enterococcus eurekensis sp. nov., isolated from a swine-manure storage pit.	Cotta MA, Whitehead TR, Falsen E, Moore E, Lawson PA	Antonie Van Leeuwenhoek	10.1007/s10482-012-9789-9	2012	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#24701

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 105069

#62596 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 61260

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68381

Automatically annotated from API rID32STR .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#91191

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID390260.1 )

* These data were automatically processed and therefore are not curated

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Enterococcus lemanii strain DSM 105069

Enterococcus lemanii strain DSM 105069

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