Name: Kushneria indalinina CG2.1
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 132885

Type strain:

Species: Kushneria indalinina

Strain Designation: CG2.1

Culture col. no.: DSM 14324, CECT 5902, CIP 109528, LMG 23625, JCM 19369

Strain history: CIP <- 2007, CECT <- 2001, A. Ramos Cormenzana, Cabo de Gata, Almeria, Spain: strain CG2.1

NCBI tax ID(s): 1122140 (strain), 184067 (species)

SI-ID 291031

LMG 23625, CECT 5902, DSM 14324, CIP 109528, JCM 19369

Section

Kushneria indalinina CG2.1 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from water sample from a solar saltern.

Gram-negative
motile
rod-shaped
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Oceanospirillales*
	Family	*Halomonadaceae*
	Genus	*Kushneria*
	Species	**Kushneria indalinina**
	Full Scientific Name (LPSN)	Kushneria indalinina (Cabrera et al. 2007) Sánchez-Porro et al. 2009

Synonym

Halomonas indalinina

Information on morphological and physiological properties Morphology

[Ref.: #31952]	Gram stain	negative
[Ref.: #120075]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %99.945

[Ref.: #31952]	Cell length	1.1-1.3 µm

[Ref.: #31952]	Cell width	0.8 µm

[Ref.: #31952]	Cell shape	rod-shaped
[Ref.: #120075]	Cell shape	rod-shaped

[Ref.: #31952]	Motility	yes
[Ref.: #120075]	Motility	yes
[Ref.: #69480]	Motility	yes Confidence in %95.564

Information on culture and growth conditions Culture and growth conditions

[Ref.: #24673]

Culture medium

BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)

[Ref.: #24673]

Culture medium growth

[Ref.: #24673]

Culture medium link

Medium recipe at MediaDive

[Ref.: #24673]

Culture medium composition

expand / minimize

[Ref.: #37757]

Culture medium

Marine agar (MA)

[Ref.: #37757]

Culture medium growth

[Ref.: #37757]

Culture medium composition

expand / minimize

[Ref.: #120075]

Culture medium

CIP Medium 13

[Ref.: #120075]

Culture medium growth

[Ref.: #120075]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #24673]	growth	28 °C
[Ref.: #31952]	growth	15-40 °C
[Ref.: #31952]	optimum	27.5 °C
[Ref.: #37757]	growth	30 °C
[Ref.: #67770]	growth	28 °C

[Ref.: #24673]	Temperature range	mesophilic
[Ref.: #31952]	Temperature range	mesophilic
[Ref.: #37757]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #31952]			optimum	7
[Ref.: #31952]			growth	5-9

Information on physiology and metabolism Physiology and metabolism

[Ref.: #31952]

Oxygen tolerance

aerobe

[Ref.: #31952]	Ability of spore formation	no
[Ref.: #69481]	Ability of spore formation	no Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.904

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #31952]		NaCl		growth	3-25	%
[Ref.: #31952]		NaCl		optimum	8.75	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #31952]	acetate ChEBI	+	carbon source
[Ref.: #68371]	amygdalin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #31952]	arabinose ChEBI	+	carbon source
[Ref.: #68371]	arbutin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #31952]	arginine ChEBI	+	carbon source
[Ref.: #31952]	cellobiose ChEBI	+	carbon source
[Ref.: #68371]	cellobiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #31952]	citrate ChEBI	+	carbon source
[Ref.: #68371]	D-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-lyxose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-ribose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-sorbitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-tagatose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	erythritol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #31952]	formate ChEBI	+	carbon source
[Ref.: #31952]	fructose ChEBI	+	carbon source
[Ref.: #68371]	galactitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #31952]	galactose ChEBI	+	carbon source
[Ref.: #68371]	gentiobiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #31952]	gluconate ChEBI	+	carbon source
[Ref.: #68371]	gluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #31952]	glucose ChEBI	+	carbon source
[Ref.: #68371]	glycerol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycogen ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	inulin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-rhamnose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-sorbose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #31952]	lactate ChEBI	+	carbon source
[Ref.: #31952]	lactose ChEBI	+	carbon source
[Ref.: #68371]	lactose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #31952]	malate ChEBI	+	carbon source
[Ref.: #31952]	maltose ChEBI	+	carbon source
[Ref.: #68371]	maltose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #31952]	mannitol ChEBI	+	carbon source
[Ref.: #31952]	mannose ChEBI	+	carbon source
[Ref.: #68371]	melezitose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	melibiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-glucopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-mannoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl beta-D-xylopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	myo-inositol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	N-acetylglucosamine ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #31952]	nitrate ChEBI	+	reduction
[Ref.: #120075]	nitrate ChEBI	+	reduction
[Ref.: #120075]	nitrite ChEBI	-	reduction
[Ref.: #68371]	Potassium 2-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	Potassium 5-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #31952]	pyruvate ChEBI	+	carbon source
[Ref.: #68371]	raffinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #31952]	ribitol ChEBI	+	carbon source
[Ref.: #68371]	ribitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	salicin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #31952]	serine ChEBI	+	carbon source
[Ref.: #68371]	starch ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #31952]	sucrose ChEBI	+	carbon source
[Ref.: #68371]	sucrose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	trehalose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	turanose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	xylitol ChEBI	-	builds acid from	* from API 50CH acid
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #120075]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	+	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	+	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	+	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #31952]	catalase	+	1.11.1.6
[Ref.: #120075]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	-		* from API zym
[Ref.: #31952]	gelatinase	+
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	-		* from API zym
[Ref.: #120075]	oxidase	-
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #120075]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® 50CH acid:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	Acid from glycerolGLY	Acid from erythritolERY	Acid from D-arabinoseDARA	Acid from L-arabinoseLARA	Acid from D-riboseRIB	Acid from D-xyloseDXYL	Acid from L-xyloseLXYL	Acid from D-adonitolADO	Acid from methyl beta-D-xylopyranosideMDX	Acid from D-galactoseGAL	Acid from D-glucoseGLU	Acid from D-fructoseFRU	Acid from D-mannoseMNE	Acid from L-sorboseSBE	Acid from L-rhamnoseRHA	Acid from dulcitolDUL	Acid from inositolINO	Acid from D-mannitolMAN	Acid from D-sorbitolSOR	Acid from methyl alpha-D-mannopyranosideMDM	Acid from methyl alpha-D-glucopyranosideMDG	Acid from N-acetyl-glucosamineNAG	Acid from amygdalinAMY	Acid from arbutinARB	Acid from esculinESC	Acid from salicinSAL	Acid from cellobioseCEL	Acid from maltoseMAL	Acid from lactoseLAC	Acid from melibioseMEL	Acid from sucroseSAC	Acid from trehaloseTRE	Acid from inulinINU	Acid from melezitoseMLZ	Acid from raffinoseRAF	Acid from starchAMD	Acid from glycogenGLYG	Acid from xylitolXLT	Acid from gentiobioseGEN	Acid from turanoseTUR	Acid from D-lyxoseLYX	Acid from D-tagatoseTAG	Acid from D-fucoseDFUC	Acid from L-fucoseLFUC	Acid from D-arabitolDARL	Acid from L-arabitolLARL	Acid from gluconateGNT	Acid from 2-Ketogluconate2KG	Acid from 5-Ketogluconate5KG
[Ref.: #120075]	11581	not determinedn.d.	-	-	-	+/-	-	+/-	-	-	-	+/-	+/-	+/-	-	-	-	-	-	-	-	-	-	-	-	-	+/-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+/-	-	-	-	-	-	-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #120075]	11932	-	+	+	-	-	+	-	-	-	-	+	-	+	+	-	+	+	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #24673]	Sample type/isolated from	water sample from a solar saltern
[Ref.: #24673]	Geographic location (country and/or sea, region)	Almería, Cabo de Gata
[Ref.: #24673]	Country	Spain
[Ref.: #24673]	Country ISO 3 Code	ESP
[Ref.: #24673]	Continent	Europe

[Ref.: #67770]	Sample type/isolated from	Water from solar saltern at Cabo de Gata
[Ref.: #67770]	Geographic location (country and/or sea, region)	Almería
[Ref.: #67770]	Country	Spain
[Ref.: #67770]	Country ISO 3 Code	ESP
[Ref.: #67770]	Continent	Europe

[Ref.: #120075]	Sample type/isolated from	Environment, Water, salt saltern
[Ref.: #120075]	Geographic location (country and/or sea, region)	Almeria
[Ref.: #120075]	Country	Spain
[Ref.: #120075]	Country ISO 3 Code	ESP
[Ref.: #120075]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	-
#Condition	#Saline	-

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AJ427627 (>99% sequence identity) for Kushneria from Microbeatlas

Aquatic Samples	20
Soil Samples	1
Animal Samples	4
Plant Samples
Total Samples	25

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #24673]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #120075]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #24673]	Halomonas indalinina partial 16S rRNA gene, type strain CG2.1T	AJ427627	1527	ENA	184067 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Kushneria indalinina DSM 14324	GCA_003385845	scaffold	GenBank	1122140 tax ID
[Ref.: #66792]	Kushneria indalinina DSM 14324	1122140.3	wgs	PATRIC	1122140 tax ID	*
[Ref.: #66792]	Kushneria indalinina DSM 14324	2756170352	draft	JGI IMG/M	184067 tax ID	*

[Ref.: #24673]	GC-content	60.9 mol%	thermal denaturation, midpoint method (Tm)
[Ref.: #31952]	GC-content	60.9 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Kushneria indalinina DSM 14324 DSM 14324 NCBI: GCA_003385845.1
[Ref.: #69481]	spore-forming	no	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Kushneria indalinina DSM 14324 PATRIC: 1122140.3
[Ref.: #69480]	motile	yes	89.321	yes
[Ref.: #69480]	flagellated	no	68.46	no
[Ref.: #69480]	gram-positive	no	98.033	yes
[Ref.: #69480]	anaerobic	no	98.618	no
[Ref.: #69480]	halophile	yes	87.719	no
[Ref.: #69480]	spore-forming	no	95.419	no
[Ref.: #69480]	thermophile	no	99.391	yes
[Ref.: #69480]	glucose-util	yes	93.748	yes
[Ref.: #69480]	aerobic	yes	89.115	yes
[Ref.: #69480]	glucose-ferment	no	52.155	no

Availability in culture collections External links

[Ref.: #24673]

Culture collection no.

DSM 14324, CECT 5902, CIP 109528, LMG 23625, JCM 19369

[Ref.: #91167]

SI-ID 291031

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Description of Kushneria aurantia gen. nov., sp. nov., a novel member of the family Halomonadaceae, and a proposal for reclassification of Halomonas marisflavi as Kushneria marisflavi comb. nov., of Halomonas indalinina as Kushneria indalinina comb. nov. and of Halomonas avicenniae as Kushneria avicenniae comb. nov.	Sanchez-Porro C, de la Haba RR, Soto-Ramirez N, Marquez MC, Montalvo-Rodriguez R, Ventosa A	Int J Syst Evol Microbiol	10.1099/ijs.0.001461-0	2009	*
Phylogeny	Halomonas indalinina sp. nov., a moderately halophilic bacterium isolated from a solar saltern in Cabo de Gata, Almeria, southern Spain.	Cabrera A, Aguilera M, Fuentes S, Incerti C, Russell NJ, Ramos-Cormenzana A, Monteoliva-Sanchez M	Int J Syst Evol Microbiol	10.1099/ijs.0.64702-0	2007	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#24673

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 14324

#31952

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #28208 (see below)

#37757 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68371

Automatically annotated from API 50CH acid .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#91167

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID291031.1 )

#120075 Collection of Institut Pasteur ; Curators of the CIP; CIP 109528

#28208

IJSEM 376 2007 ( DOI 10.1099/ijs.0.64702-0 , PubMed 17267982 )

* These data were automatically processed and therefore are not curated

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Kushneria indalinina DSM 14324 DSM 14324

Kushneria indalinina DSM 14324

Change proposal

Change proposal