Amycolatopsis ultiminotia (DSM 45180, JCM 16898, NRRL B-24662)

Name: Amycolatopsis ultiminotia (DSM 45180, JCM 16898, NRRL B-24662)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 13268

Type strain

Strain Designation RP-AC36

Culture col. no. DSM 45180 JCM 16898 NRRL B-24662 RP-AC36

NCBI tax ID(s) 543629

Special Collections DSMZ JCM Wink compendium Literature strains

History <- S. D. Lee; RP-Ac 36 DSM 45180 <-- S. D. Lee RP-Ac 36.

Links

Amycolatopsis ultiminotia RP-AC36 is an aerobe, mesophilic, Gram-positive prokaryote that builds an aerial mycelium and was isolated from rhizosphere of a cliff-associated plant .

Gram-positive rod-shaped aerobe mesophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 399558

JCM 16898,DSM 45180

@ref 20215

Last LPSN update 2026-02-09 11:21:05

Domain Bacteria

Phylum Actinomycetota

Class Actinomycetes

Order Pseudonocardiales

Family Pseudonocardiaceae

Genus Amycolatopsis

Species Amycolatopsis ultiminotia

Full scientific name Amycolatopsis ultiminotia Lee 2009

Morphology

Cell morphology

@ref	Gram stain	Cell shape
29112	positive	rod-shaped

Colony morphology

@ref	Colony color	Incubation period	Medium used
20176	Sand yellow (1002)	10-14 days	ISP 2
20176	Ivory (1014)	10-14 days	ISP 3
20176	Ivory (1014)	10-14 days	ISP 7

Multicellular morphology

@ref	Complex name	Complex color	Medium name
20176	Aerial Mycelium	Light ivory	ISP 2
20176			ISP 3
20176			IPS 7

Pigmentation

29112

Productionyes

Multimedia

@ref:	15643
multimedia content:	DSM_45180.jpg
multimedia.multimedia content:	https://www.dsmz.de/microorganisms/photos/DSM_45180.jpg
caption:	Medium 553 28°C
intellectual property rights:	© Leibniz-Institut DSMZ
manual_annotation:	1

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
15643	GPHF-MEDIUM (DSMZ Medium 553)	Medium recipe at MediaDive	Name: GPHF-MEDIUM (DSMZ Medium 553) Composition: Agar 20.0 g/l Glucose 10.0 g/l Beef extract 5.0 g/l Yeast extract 5.0 g/l Casein peptone 5.0 g/l CaCl2 x 2 H2O 0.74 g/l Distilled water
15643	GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)	Medium recipe at MediaDive	Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
20176	ISP 2		Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
20176	ISP 3		Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
20176	ISP 7		Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
15643	positive	growth	28	mesophilic
20176	positive	optimum	28	mesophilic
29112	positive	growth	10-37
29112	positive	optimum	28.5	mesophilic
67770	positive	growth	28	mesophilic

Culture pH

@ref	Ability	Type	PH	PH range
29112	positive	optimum	7.1
29112	positive	growth	5.1-12.1	alkaliphile

Physiology and metabolism

Oxygen tolerance

29112

Oxygen toleranceaerobe

Halophily

@ref	Salt	Growth	Tested relation	Concentration
29112	NaCl	positive	growth	0-5 %
29112	NaCl	positive	optimum	2.5 %

Observation

67770

Observationquinones: MK-9(H₄)

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
20176	22599 ChEBI	arabinose	+/-
29112	17057 ChEBI	cellobiose	+	carbon source
20176	62968 ChEBI	cellulose	-
29112	4853 ChEBI	esculin	+	hydrolysis
20176	28757 ChEBI	fructose	+
20176	17234 ChEBI	glucose	+
29112	17234 ChEBI	glucose	+	carbon source
29112	17754 ChEBI	glycerol	+	carbon source
29112	17716 ChEBI	lactose	+	carbon source
29112	17306 ChEBI	maltose	+	carbon source
20176	29864 ChEBI	mannitol	+
20176	17268 ChEBI	myo-inositol	+
20176	16634 ChEBI	raffinose	+/-
20176	26546 ChEBI	rhamnose	+
29112	26546 ChEBI	rhamnose	+	carbon source
20176	17992 ChEBI	sucrose	+/-
20176	18222 ChEBI	xylose	+/-
29112	18222 ChEBI	xylose	+	carbon source

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68382	alpha-chymotrypsin	+	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	+	3.2.1.22	from API zym
68382	alpha-glucosidase	-	3.2.1.20	from API zym
68382	alpha-mannosidase	+	3.2.1.24	from API zym
68382	beta-galactosidase	+	3.2.1.23	from API zym
68382	beta-glucosidase	-	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
29112	catalase	+	1.11.1.6
68382	cystine arylamidase	+	3.4.11.3	from API zym
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	+		from API zym
68382	N-acetyl-beta-glucosaminidase	+	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
68382	trypsin	-	3.4.21.4	from API zym
68382	valine arylamidase	+		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
20176	not determinedn.d.	+	+	+	+	+	+	+	-	+	+	+	+	+	-	-	-	+	+	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Host	#Plants	#Herbaceous plants (Grass,Crops)
#Host Body-Site	#Plant	#Rhizosphere

Isolation

@ref	Sample type	Host species	Geographic location	Country	Country ISO 3 Code	Continent
15643	rhizosphere of a cliff-associated plant (Peucedanum japonicum Thunb.)	Peucedanum japonicum	Jeju, Mara Island	Republic of Korea	KOR	Asia
67770	Rhizosphere of a cliff-associated plant, Peucedanum japonicum Thunb.	Peucedanum japonicum	Mara Island, Jeju	Republic of Korea	KOR	Asia

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
15643	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	NCBI tax ID	Score
124043	ASM3953692v1 assembly for Amycolatopsis ultiminotia JCM 16898	scaffold	GCA_039536925	543629	57.28

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
15643	Amycolatopsis ultiminotia partial 16S rRNA gene, type strain RP-AC36T	FM177516	1413		543629

GC content

@ref	GC-content (mol%)	Method
15643	67.5	high performance liquid chromatography (HPLC)
29112	67.5

Genome-based predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Amycolatopsis cihanbeyliensis sp. nov., a halotolerant actinomycete isolated from a salt mine.	Tatar D, Sazak A, Guven K, Cetin D, Sahin N	Int J Syst Evol Microbiol	10.1099/ijs.0.050963-0	2013
Phylogeny	Amycolatopsis jiangsuensis sp. nov., a novel endophytic actinomycete isolated from a coastal plant in Jiangsu, China.	Xing K, Liu W, Zhang YJ, Bian GK, Zhang WD, Tamura T, Lee JS, Qin S, Jiang JH	Antonie Van Leeuwenhoek	10.1007/s10482-012-9823-y	2012
Phylogeny	Amycolatopsis ultiminotia sp. nov., isolated from rhizosphere soil, and emended description of the genus Amycolatopsis.	Lee SD	Int J Syst Evol Microbiol	10.1099/ijs.0.006577-0	2009

References

#15643	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 45180
#20176	Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#25539	IJSEM 1401 2009 ( DOI 10.1099/ijs.0.006577-0 , PubMed 19502324 )
#29112	Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #25539
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68382	Automatically annotated from API zym .
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Amycolatopsis ultiminotia (DSM 45180, JCM 16898, NRRL B-24662)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Multicellular morphology

Pigmentation

Multimedia

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Halophily

Observation

Metabolite utilization

Enzymes

API zym

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_039536925

16S sequences

GC content

Genome-based predictions

Literature

Literature

References

BacDive

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