Name: Brachybacterium aquaticum KWS-1
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 132661

Type strain:

Species: Brachybacterium aquaticum

Strain Designation: KWS-1

Culture col. no.: DSM 28796, JCM 30059, MTCC 11836

Strain history: S. Mayilraj; MTCC, India; KWS-1.

NCBI tax ID(s): 1432564 (species)

Special Collections

Section

Brachybacterium aquaticum KWS-1 is an aerobe, mesophilic, Gram-positive bacterium that forms circular colonies and has a pale-yellowish pigmentation.

coccus-shaped
colony-forming
Gram-positive
pigmented
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	09-12-2024 (DD-MM-YYYY)
	Domain	*Bacteria*
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Micrococcales*
	Family	*Dermabacteraceae*
	Genus	*Brachybacterium*
	Species	**Brachybacterium aquaticum**
	Full Scientific Name (LPSN)	Brachybacterium aquaticum Kaur et al. 2016

Information on morphological and physiological properties Morphology

[Ref.: #24973]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %92.098

[Ref.: #24973]	Cell shape	coccus-shaped

[Ref.: #69480]	Motility	no Confidence in %91

[Ref.: #24973]	Colony size	0.4-3.0 mm
[Ref.: #24973]	Colony color	straw-yellow
[Ref.: #24973]	Colony shape	circular
[Ref.: #24973]	Cultivation medium used	TSA (Trypticase soy agar)

[Ref.: #24973]	Pigment production	yes
[Ref.: #24973]	Pigmentation color	pale-yellowish

Information on culture and growth conditions Culture and growth conditions

[Ref.: #24973]

Culture medium

TSA (Trypticase soy agar)

[Ref.: #24973]

Culture medium growth

		Temperature
[Ref.: #24973]	optimum	30 °C
[Ref.: #24973]	growth	20.0-37.0 °C
[Ref.: #67770]	growth	28 °C

	Kind of pH		pH
[Ref.: #24973]		optimum	8
[Ref.: #24973]		growth	7.0-11.0
[Ref.: #24973]	no	growth	5
[Ref.: #24973]		growth	11

Information on physiology and metabolism Physiology and metabolism

[Ref.: #24973]

Oxygen tolerance

aerobe

[Ref.: #69481]

Ability of spore formation

no Confidence in %99

	Salt		Salt conc.
[Ref.: #24973]	NaCl	maximum	8	%(w/v)
[Ref.: #24973]	NaCl	growth	5	%(w/v)
[Ref.: #24973]	NaCl	growth	7	%(w/v)

[Ref.: #24973]	Murein short key	A31
[Ref.: #24973]	Murein types	A1gamma m-Dpm-direct

[Ref.: #67770]

Observation

quinones: MK-7

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #24973]	(R)-amygdalin ChEBI	-	builds acid from
[Ref.: #24973]	casein	-	hydrolysis
[Ref.: #24973]	cellobiose ChEBI	-	builds acid from
[Ref.: #24973]	cyclodextrin ChEBI	-	builds acid from
[Ref.: #24973]	D-galactose ChEBI	-	builds acid from
[Ref.: #24973]	D-mannitol ChEBI	-	builds acid from
[Ref.: #24973]	D-mannose ChEBI	-	builds acid from
[Ref.: #24973]	D-ribose ChEBI	-	builds acid from
[Ref.: #24973]	D-sorbitol ChEBI	-	builds acid from
[Ref.: #24973]	D-xylose ChEBI	-	builds acid from
[Ref.: #24973]	fructose ChEBI	+	builds acid from
[Ref.: #24973]	galactose ChEBI	+	builds acid from
[Ref.: #24973]	gelatin ChEBI	-	hydrolysis
[Ref.: #24973]	glucose ChEBI	+	builds acid from
[Ref.: #24973]	lactose ChEBI	-	builds acid from
[Ref.: #24973]	maltose ChEBI	-	builds acid from
[Ref.: #24973]	methyl beta-D-glucopyranoside ChEBI	-	builds acid from
[Ref.: #24973]	N-acetylglucosamine ChEBI	-	builds acid from
[Ref.: #24973]	nitrate ChEBI	-	reduction
[Ref.: #24973]	O-nitrophenyl-beta-D-galactopyranosid	-	hydrolysis
[Ref.: #24973]	pullulan ChEBI	-	builds acid from
[Ref.: #24973]	raffinose ChEBI	-	builds acid from
[Ref.: #24973]	rhamnose ChEBI	-	builds acid from
[Ref.: #24973]	salicin ChEBI	-	builds acid from
[Ref.: #24973]	starch ChEBI	+	hydrolysis
[Ref.: #24973]	sucrose ChEBI	-	builds acid from
[Ref.: #24973]	sucrose ChEBI	+	builds acid from
[Ref.: #24973]	trehalose ChEBI	-	builds acid from
[Ref.: #24973]	tryptophan ChEBI	-	energy source
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Antibiotic resistance
[Ref.: #24973]	bacitracin ChEBI	no
[Ref.: #24973]	novobiocin ChEBI	no
[Ref.: #24973]	optochin ChEBI	no
[Ref.: #24973]	polymyxin b ChEBI	no

	Metabolite	Production
[Ref.: #24973]	acetoin ChEBI	no
[Ref.: #24973]	hydrogen sulfide ChEBI	no
[Ref.: #24973]	indole ChEBI	no

	Physiological tests	Metabolite	Voges-Proskauer-test	Indole test
[Ref.: #24973]		acetoin ChEBI	-
[Ref.: #24973]		indole ChEBI		-

	Enzyme	Enzyme activity	EC number
[Ref.: #24973]	alanine arylamidase	+	3.4.11.2
[Ref.: #24973]	alpha-galactosidase	-	3.2.1.22
[Ref.: #24973]	alpha-glucosidase	-	3.2.1.20
[Ref.: #24973]	alpha-mannosidase	-	3.2.1.24
[Ref.: #24973]	arginine dihydrolase	+	3.5.3.6
[Ref.: #24973]	beta-galactosidase	+	3.2.1.23
[Ref.: #24973]	beta-glucuronidase	-	3.2.1.31
[Ref.: #24973]	catalase	+	1.11.1.6
[Ref.: #24973]	cytochrome oxidase	-	1.9.3.1
[Ref.: #24973]	L-aspartate arylamidase	-	3.4.11.21
[Ref.: #24973]	phosphatase	-
[Ref.: #24973]	proline-arylamidase	+	3.4.11.5
[Ref.: #24973]	pyrrolidonyl arylamidase	-	3.4.19.3
[Ref.: #24973]	tripeptide aminopeptidase	-	3.4.11.4
[Ref.: #24973]	tryptophan deaminase	-	4.1.99.1
[Ref.: #24973]	tyrosine arylamidase	+
[Ref.: #24973]	urease	-	3.5.1.5
	Only first 10 entries are displayed. Click here to see all.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #24450]	Sample type/isolated from	seawater
[Ref.: #24450]	Geographic location (country and/or sea, region)	Kanyakumari coastal region of the Bay of Bengal, Kovalam (8.0914651° N 77.5184862° E)
[Ref.: #24450]	Country	India
[Ref.: #24450]	Country ISO 3 Code	IND
[Ref.: #24450]	Continent	Asia
[Ref.: #24450]	Geographic location	8.0915°/77.5185°

[Ref.: #67770]	Sample type/isolated from	Seawater from the Kanyakumari coastal region of the Bay of Bengal
[Ref.: #67770]	Country	India
[Ref.: #67770]	Country ISO 3 Code	IND
[Ref.: #67770]	Continent	Asia

[Ref.: #24973]	Enrichment culture	TSA (Trypticase soy agar)(HiMedia)
[Ref.: #24973]	Isolation/enrichment procedure	dilution-plate technique
* marker position based on {}

Isolation sources categories

#Environmental

#Aquatic

#Marine

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence KF701619 (>99% sequence identity) for Brachybacterium aquaticum from Microbeatlas

Aquatic Samples	40
Soil Samples	11
Animal Samples	173
Plant Samples	9
Total Samples	233

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #24450]	Brachybacterium aquaticum strain KWS1 16S ribosomal RNA gene, partial sequence	KF701619	1423	GenBank ENA	1432564 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Brachybacterium aquaticum DSM 28796	GCA_014204755	contig	GenBank ENA	1432564 tax ID	*
[Ref.: #66792]	Brachybacterium aquaticum strain DSM 28796	1432564.3	wgs	PATRIC	1432564 tax ID	*
[Ref.: #66792]	Brachybacterium aquaticum DSM 28796	2868155942	draft	JGI IMG/M	1432564 tax ID	*

[Ref.: #24973]	GC-content	71.8 mol%	Thermal denaturation, fluorometry
[Ref.: #24450]	GC-content	71.8 mol%	thermal denaturation, midpoint method (Tm)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Brachybacterium aquaticum strain DSM 28796 PATRIC: 1432564.3
[Ref.: #69481]	spore-forming	spore-formingⓘ	no	99	no

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Brachybacterium aquaticum strain DSM 28796 PATRIC: 1432564.3
[Ref.: #69480]	gram-positive	gram-positiveⓘ	yes	92.098	yes
[Ref.: #69480]	anaerobic	anaerobicⓘ	no	92.411	yes
[Ref.: #69480]	spore-forming	spore-formingⓘ	no	79.017	no
[Ref.: #69480]	aerobic	aerobicⓘ	yes	79.112	yes
[Ref.: #69480]	thermophilic	thermophileⓘ	no	95	yes
[Ref.: #69480]	flagellated	motile2+ⓘ	no	91	no

Availability in culture collections External links

[Ref.: #24450]

Culture collection no.

DSM 28796, JCM 30059, MTCC 11836

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Brachybacterium aquaticum sp. nov., a novel actinobacterium isolated from seawater.	Kaur G, Kumar N, Mual P, Kumar A, Kumar RM, Mayilraj S	Int J Syst Evol Microbiol	10.1099/ijsem.0.001414	2016	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#24450

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 28796

#24973

G. K. Kaur, Narender,Mual, Poonam,Kumar, Anand,Kumar, Rajendran Mathan,Mayilraj, Shanmugam: Brachybacterium aquaticum sp. nov., a novel actinobacterium isolated from seawater. IJSEM 66: 4705 - 4710 2016 ( DOI 10.1099/ijsem.0.001414 , PubMed 27514821 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

* These data were automatically processed and therefore are not curated

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Brachybacterium aquaticum strain DSM 28796

Brachybacterium aquaticum strain DSM 28796

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