Name: Amycolatopsis jejuensis N7-3
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 13262

Type strain:

Species: Amycolatopsis jejuensis

Strain Designation: N7-3

Culture col. no.: DSM 45042, JCM 13280, NRRL B-24427, CIP 109183, NCIMB 14306

Strain history: S. D. Lee N7-3.

NCBI tax ID(s): 330084 (species)

Section

Amycolatopsis jejuensis N7-3 is an aerobe, spore-forming, mesophilic bacterium that was isolated from dried bat dung in natural cave.

Gram-positive
rod-shaped
spore-forming
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	07-12-2022 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Pseudonocardiales*
	Family	*Pseudonocardiaceae*
	Genus	*Amycolatopsis*
	Species	**Amycolatopsis jejuensis**
	Full Scientific Name (LPSN)	Amycolatopsis jejuensis Lee 2006

Information on morphological and physiological properties Morphology

[Ref.: #31605]	Gram stain	positive
[Ref.: #31605]	Cell shape	rod-shaped

[Ref.: #31605]

Pigment production

yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #12269]

Culture medium

GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)

[Ref.: #12269]

Culture medium growth

[Ref.: #12269]

Culture medium link

Medium recipe at MediaDive

[Ref.: #12269]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #12269]	growth	28 °C
[Ref.: #31605]	growth	10-30 °C
[Ref.: #31605]	optimum	10-30 °C
[Ref.: #67770]	growth	28 °C

[Ref.: #12269]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #31605]

Oxygen tolerance

aerobe

[Ref.: #31605]

Ability of spore formation

yes

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #31605]		NaCl		growth	2	%
[Ref.: #31605]		NaCl		optimum	2	%

[Ref.: #31605]	Observation	aggregates in chains
[Ref.: #67770]	Observation	quinones: MK-9(H₄)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68369]	adipate ChEBI	+	assimilation	* from API 20NE
[Ref.: #31605]	arabinose ChEBI	+	carbon source
[Ref.: #68369]	arginine ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	-	fermentation	* from API 20NE
[Ref.: #68369]	D-mannitol ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	D-mannose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	decanoate ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	esculin ChEBI	+	hydrolysis	* from API 20NE
[Ref.: #31605]	fructose ChEBI	+	carbon source
[Ref.: #31605]	galactose ChEBI	+	carbon source
[Ref.: #68369]	gelatin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	gluconate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	L-arabinose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	malate ChEBI	+	assimilation	* from API 20NE
[Ref.: #31605]	mannitol ChEBI	+	carbon source
[Ref.: #31605]	mannose ChEBI	+	carbon source
[Ref.: #68369]	N-acetylglucosamine ChEBI	+	assimilation	* from API 20NE
[Ref.: #31605]	nitrate ChEBI	+	reduction
[Ref.: #68369]	nitrate ChEBI	-	reduction	* from API 20NE
[Ref.: #31605]	ribitol ChEBI	+	carbon source
[Ref.: #68369]	tryptophan ChEBI	-	energy source	* from API 20NE
[Ref.: #68369]	urea ChEBI	-	hydrolysis	* from API 20NE
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite
[Ref.: #31605]		hydrogen sulfide ChEBI
[Ref.: #68369]		indole ChEBI	* from API 20NE

	Physiological tests	Metabolite	Indole test
[Ref.: #68369]		indole ChEBI	-	* from API 20NE

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68369]	arginine dihydrolase	-	3.5.3.6	* from API 20NE
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68369]	beta-glucosidase	+	3.2.1.21	* from API 20NE
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #31605]	catalase	+	1.11.1.6
[Ref.: #68369]	gelatinase	-		* from API 20NE
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	+	3.2.1.52	* from API zym
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #31605]	urease	+	3.5.1.5
[Ref.: #68369]	urease	-	3.5.1.5	* from API 20NE
	Only first 10 entries are displayed. Click here to see all.

API® 20NE:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitratesNO3	Indole productionTRP	Fermentation of D-glucoseGLU_ Ferm	Arginine dihydrolaseADH (Arg)	Urease/urea hydrolysisURE	Esculin hydrolysis/beta-GlucosidaseESC	Gelatinase/gelatin hydrolysisGEL	beta-GalactosidasePNPG	Assimilation of D-glucoseGLU_ Assim	Assimilation of L-arabinoseARA	Assimilation of D-mannoseMNE	Assimilation of D-mannitolMAN	Assimilation of N-acetyl-glucosamineNAG	Assimilation of maltoseMAL	Assimilation of gluconateGNT	Assimilation of capric acidCAP	Assimilation adipic acidADI	Assimilation of malic acidMLT	Assimilation of citric acidCIT	Assimilation of phenyl-acetic acidPAC	Cytochrome oxidaseOX
[Ref.: #12269]	22750	-	-	-	-	-	+	-	-	+	-	+	+	+	+/-	+	-	+	+	+	+/-	not determinedn.d.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #12269]	6507	-	+	+/-	+/-	-	+	+/-	+/-	-	-	+	+/-	-	-	-	+	-	+	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #12269]	Sample type/isolated from	dried bat dung in natural cave
[Ref.: #12269]	Geographic location (country and/or sea, region)	Jeju
[Ref.: #12269]	Country	Republic of Korea
[Ref.: #12269]	Country ISO 3 Code	KOR
[Ref.: #12269]	Continent	Asia

[Ref.: #67770]	Sample type/isolated from	Dried bat dung inside a natural cave
[Ref.: #67770]	Geographic location (country and/or sea, region)	Jeju Island
[Ref.: #67770]	Country	Republic of Korea
[Ref.: #67770]	Country ISO 3 Code	KOR
[Ref.: #67770]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Environmental	#Terrestrial	-
#Host	#Mammals	-
#Host Body Product	#Gastrointestinal tract	#Feces (Stool)

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence DQ000200 (>99% sequence identity) for Amycolatopsis jejuensis subclade from Microbeatlas

Aquatic Samples	18
Soil Samples	232
Animal Samples	61
Plant Samples	41
Total Samples	352

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #12269]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #12269]	Amycolatopsis jejuensis strain N7-3 16S ribosomal RNA gene, partial sequence	DQ000200	1514	ENA	330084 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Amycolatopsis jejuensis NRRL B-24427	GCA_000717335	contig	GenBank	330084 tax ID

[Ref.: #12269]	GC-content	71.7 mol%
[Ref.: #67770]	GC-content	71.7 mol%	high performance liquid chromatography (HPLC)
[Ref.: #67770]	GC-content	68.6 mol%	genome sequence analysis

Availability in culture collections External links

[Ref.: #12269]

Culture collection no.

DSM 45042, JCM 13280, NRRL B-24427, CIP 109183, NCIMB 14306

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Amycolatopsis panacis sp. nov., isolated from Panax notoginseng rhizospheric soil.	Peng G, Xiong DS, Li LC, Hu JY, Bao S, Chen YW, Li YQ, Xu LH, Miao CP, Zhao LX	Int J Syst Evol Microbiol	10.1099/ijsem.0.003202	2019	*
Phylogeny	Amycolatopsis jiangsuensis sp. nov., a novel endophytic actinomycete isolated from a coastal plant in Jiangsu, China.	Xing K, Liu W, Zhang YJ, Bian GK, Zhang WD, Tamura T, Lee JS, Qin S, Jiang JH	Antonie Van Leeuwenhoek	10.1007/s10482-012-9823-y	2012	*
Phylogeny	Amycolatopsis jejuensis sp. nov. and Amycolatopsis halotolerans sp. nov., novel actinomycetes isolated from a natural cave.	Lee SD	Int J Syst Evol Microbiol	10.1099/ijs.0.63881-0	2006	*

References

#12269

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 45042

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#31605

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #27893 (see below)

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68369

Automatically annotated from API 20NE .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#27893

IJSEM 549 2006 ( DOI 10.1099/ijs.0.63881-0 , PubMed 16514025 )

* These data were automatically processed and therefore are not curated

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