Name: Amycolatopsis halotolerans N4-6
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 13261

Type strain:

Species: Amycolatopsis halotolerans

Strain Designation: N4-6

Culture col. no.: DSM 45041, JCM 13279, NRRL B-24428, CIP 109182, NCIMB 14386

Strain history: CIP <- 2006, JCM <- S. D. Lee: strain N4-6

NCBI tax ID(s): 330083 (species)

SI-ID 290956

JCM 13279, NRRL B-24428, DSM 45041, CIP 109182

Section

Amycolatopsis halotolerans N4-6 is an aerobe, spore-forming, mesophilic bacterium that was isolated from soil sample inside a natural cave.

Gram-positive
rod-shaped
spore-forming
aerobe
mesophilic
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Pseudonocardiales*
	Family	*Pseudonocardiaceae*
	Genus	*Amycolatopsis*
	Species	**Amycolatopsis halotolerans**
	Full Scientific Name (LPSN)	Amycolatopsis halotolerans Lee 2006

Information on morphological and physiological properties Morphology

[Ref.: #31605]	Gram stain	positive
[Ref.: #119822]	Gram stain	positive

[Ref.: #31605]	Cell shape	rod-shaped
[Ref.: #119822]	Cell shape	rod-shaped

[Ref.: #119822]	Motility	no

[Ref.: #31605]

Pigment production

yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #12268]

Culture medium

GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)

[Ref.: #12268]

Culture medium growth

[Ref.: #12268]

Culture medium link

Medium recipe at MediaDive

[Ref.: #12268]

Culture medium composition

expand / minimize

[Ref.: #119822]

Culture medium

CIP Medium 115

[Ref.: #119822]

Culture medium growth

[Ref.: #119822]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #119822]

Culture medium

CIP Medium 737

[Ref.: #119822]

Culture medium growth

[Ref.: #119822]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #12268]	growth	28 °C
[Ref.: #31605]	growth	10-37 °C
[Ref.: #31605]	optimum	10-37 °C
[Ref.: #67770]	growth	28 °C

[Ref.: #12268]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #31605]			optimum	7
[Ref.: #31605]			growth	7

Information on physiology and metabolism Physiology and metabolism

[Ref.: #31605]	Oxygen tolerance	aerobe
[Ref.: #119822]	Oxygen tolerance	obligate aerobe

[Ref.: #31605]

Ability of spore formation

yes

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #31605]		NaCl		growth	7	%
[Ref.: #31605]		NaCl		optimum	7	%

[Ref.: #31605]	Observation	aggregates in chains
[Ref.: #67770]	Observation	quinones: MK-9(H₄), MK-9(H₆), MK-9(H₈)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #31605]	cellobiose ChEBI	+	carbon source
[Ref.: #31605]	fructose ChEBI	+	carbon source
[Ref.: #31605]	galactose ChEBI	+	carbon source
[Ref.: #31605]	glycerol ChEBI	+	carbon source
[Ref.: #31605]	mannitol ChEBI	+	carbon source
[Ref.: #31605]	mannose ChEBI	+	carbon source
[Ref.: #31605]	melibiose ChEBI	+	carbon source
[Ref.: #31605]	nitrate ChEBI	+	reduction
[Ref.: #119822]	nitrate ChEBI	-	reduction
[Ref.: #119822]	nitrite ChEBI	-	reduction
[Ref.: #31605]	ribitol ChEBI	+	carbon source
[Ref.: #31605]	ribose ChEBI	+	carbon source
[Ref.: #31605]	sucrose ChEBI	+	carbon source
[Ref.: #31605]	xylose ChEBI	+	carbon source
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #119822]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	+	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	+	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	+	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	+	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #31605]	catalase	+	1.11.1.6
[Ref.: #119822]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #31605]	cytochrome oxidase	+	1.9.3.1
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	+		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	+	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #119822]	oxidase	-
[Ref.: #68382]	trypsin	+	3.4.21.4	* from API zym
[Ref.: #31605]	urease	+	3.5.1.5
[Ref.: #119822]	urease	+	3.5.1.5
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #119822]	11701	-	+	+	+	+	+	-	-	+	+	+	+	-	+	-	+	+	+	+	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #12268]	Sample type/isolated from	soil sample inside a natural cave
[Ref.: #12268]	Geographic location (country and/or sea, region)	Jeju Island
[Ref.: #12268]	Country	Republic of Korea
[Ref.: #12268]	Country ISO 3 Code	KOR
[Ref.: #12268]	Continent	Asia

[Ref.: #67770]	Sample type/isolated from	Soil inside a natural cave
[Ref.: #67770]	Geographic location (country and/or sea, region)	Jeju Island
[Ref.: #67770]	Country	Republic of Korea
[Ref.: #67770]	Country ISO 3 Code	KOR
[Ref.: #67770]	Continent	Asia

[Ref.: #119822]	Sample type/isolated from	Environment, Soil sample from a natural cave
[Ref.: #119822]	Geographic location (country and/or sea, region)	Jeju Island
[Ref.: #119822]	Country	Republic of Korea
[Ref.: #119822]	Country ISO 3 Code	KOR
[Ref.: #119822]	Continent	Asia
[Ref.: #119822]	Isolation date	2002-10-01
* marker position based on {}

Isolation sources categories

#Environmental	#Terrestrial	#Geologic
#Environmental	#Terrestrial	#Soil

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #12268]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #119822]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #12268]	Amycolatopsis halotolerans strain N4-6 16S ribosomal RNA gene, partial sequence	DQ000196	1512	ENA	330083 tax ID

[Ref.: #12268]	GC-content	72.5 mol%
[Ref.: #67770]	GC-content	72.5 mol%	high performance liquid chromatography (HPLC)

Availability in culture collections External links

[Ref.: #12268]

Culture collection no.

DSM 45041, JCM 13279, NRRL B-24428, CIP 109182, NCIMB 14386

[Ref.: #82465]

SI-ID 290956

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Amycolatopsis jejuensis sp. nov. and Amycolatopsis halotolerans sp. nov., novel actinomycetes isolated from a natural cave.	Lee SD	Int J Syst Evol Microbiol	10.1099/ijs.0.63881-0	2006	*

References

#12268

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 45041

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#31605

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #27893 (see below)

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68382

Automatically annotated from API zym .

#82465

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID290956.1 )

#119822 Collection of Institut Pasteur ; Curators of the CIP; CIP 109182

#27893

IJSEM 549 2006 ( DOI 10.1099/ijs.0.63881-0 , PubMed 16514025 )

* These data were automatically processed and therefore are not curated

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