Name: Tsukamurella serpentis HKU54
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 132604

Type strain:

Species: Tsukamurella serpentis

Strain Designation: HKU54

Culture col. no.: DSM 100915, JCM 31017

Strain history: J. L. L. Teng; Dept. of Microbiol., Univ. of Hong Kong, China; HKU54.

NCBI tax ID(s): 1750603 (species)

Other strains from Tsukamurella serpentis

T. serpentis HKU55, DSM 100916, JCM 31018

Section

Tsukamurella serpentis HKU54 is an aerobe, thermophilic, Gram-positive bacterium that forms irregular colonies and was isolated from oral cavity of a healthy Chinese cobra Naja atra.

colony-forming
Gram-positive
rod-shaped
aerobe
thermophilic
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	07-12-2022 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Mycobacteriales*
	Family	*Tsukamurellaceae*
	Genus	*Tsukamurella*
	Species	**Tsukamurella serpentis**
	Full Scientific Name (PNU)	Tsukamurella serpentis Tang et al. 2016

Information on morphological and physiological properties Morphology

[Ref.: #43103]	Gram stain	positive
[Ref.: #43103]	Cell shape	rod-shaped
[Ref.: #43103]	Motility	no

[Ref.: #43103]	Colony size	2 mm
[Ref.: #43103]	Colony color	cream-to-yellow
[Ref.: #43103]	Colony shape	irregular
[Ref.: #43103]	Incubation period	2 days
[Ref.: #43103]	Cultivation medium used	Columbia agar with 5 % defibrinated sheep blood agar

Multimedia content

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Caption:

Medium 693 25°C

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Intellectual property rights:

Information on culture and growth conditions Culture and growth conditions

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Culture medium

Columbia agar with 5 % defibrinated sheep blood agar

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Culture medium growth

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Culture medium

brain-heart infusion agar

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Culture medium growth

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Culture medium

nutritient agar (NA)

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Culture medium growth

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Culture medium

trypticase soy agar (TSA)

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Culture medium growth

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Culture medium

chocolate agar

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Culture medium growth

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Culture medium

MacConkey agar

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Culture medium growth

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Culture medium

TRYPTICASE SOY BROTH AGAR+BLOOD (DSMZ Medium 535a)

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Culture medium growth

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Culture medium link

Medium recipe provided by DSMZ

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Culture medium composition

expand / minimize

	Kind of temperature		Temperature
[Ref.: #24393]		growth	25 °C
[Ref.: #43103]	no	growth	42 °C
[Ref.: #43103]		growth	10-37 °C
[Ref.: #67770]		growth	28 °C

[Ref.: #24393]	Temperature range	mesophilic
[Ref.: #43103]	Temperature range	thermophilic
[Ref.: #67770]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

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Oxygen tolerance

aerobe

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Ability of spore formation

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #43103]		NaCl		maximum	7	%(w/v)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #43103]	2-dehydro-D-gluconate ChEBI	-	assimilation
[Ref.: #43103]	5-dehydro-D-gluconate ChEBI	-	assimilation
[Ref.: #43103]	amygdalin ChEBI	-	assimilation
[Ref.: #43103]	arbutin ChEBI	-	assimilation
[Ref.: #43103]	cellobiose ChEBI	-	assimilation
[Ref.: #43103]	D-arabinose ChEBI	-	assimilation
[Ref.: #43103]	D-arabitol ChEBI	+	assimilation
[Ref.: #43103]	D-fructose ChEBI	+	assimilation
[Ref.: #43103]	D-fucose ChEBI	-	assimilation
[Ref.: #43103]	D-galactose ChEBI	+	assimilation
[Ref.: #43103]	D-glucose ChEBI	+	assimilation
[Ref.: #43103]	D-lyxose ChEBI	-	assimilation
[Ref.: #43103]	D-mannitol ChEBI	+	assimilation
[Ref.: #43103]	D-ribose ChEBI	-	assimilation
[Ref.: #43103]	D-sorbitol ChEBI	+	assimilation
[Ref.: #43103]	D-tagatose ChEBI	-	assimilation
[Ref.: #43103]	D-xylose ChEBI	-	assimilation
[Ref.: #43103]	esculin ferric citrate	-	assimilation
[Ref.: #43103]	galactitol ChEBI	-	assimilation
[Ref.: #43103]	gentiobiose ChEBI	-	assimilation
[Ref.: #43103]	glycogen ChEBI	-	assimilation
[Ref.: #43103]	inulin ChEBI	-	assimilation
[Ref.: #43103]	L-arabinose ChEBI	-	assimilation
[Ref.: #43103]	L-arabitol ChEBI	-	assimilation
[Ref.: #43103]	L-fucose ChEBI	-	assimilation
[Ref.: #43103]	L-rhamnose ChEBI	-	assimilation
[Ref.: #43103]	L-sorbose ChEBI	-	assimilation
[Ref.: #43103]	L-xylose ChEBI	-	assimilation
[Ref.: #43103]	lactose ChEBI	-	assimilation
[Ref.: #43103]	maltose ChEBI	+	assimilation
[Ref.: #43103]	melezitose ChEBI	-	assimilation
[Ref.: #43103]	melibiose ChEBI	-	assimilation
[Ref.: #43103]	methyl alpha-D-mannoside ChEBI	-	assimilation
[Ref.: #43103]	methyl D-glucoside ChEBI	-	assimilation
[Ref.: #43103]	myo-inositol ChEBI	+	assimilation
[Ref.: #43103]	N-acetylglucosamine ChEBI	-	assimilation
[Ref.: #43103]	potassium gluconate ChEBI	-	assimilation
[Ref.: #43103]	raffinose ChEBI	-	assimilation
[Ref.: #43103]	ribitol ChEBI	-	assimilation
[Ref.: #43103]	salicin ChEBI	-	assimilation
[Ref.: #43103]	starch ChEBI	-	assimilation
[Ref.: #43103]	sucrose ChEBI	+	assimilation
[Ref.: #43103]	trehalose ChEBI	+	assimilation
[Ref.: #43103]	turanose ChEBI	+	assimilation
[Ref.: #43103]	tyrosine ChEBI	-	hydrolysis
[Ref.: #43103]	xanthine ChEBI	-	hydrolysis
[Ref.: #43103]	xylitol ChEBI	+	assimilation
	Only first 10 entries are displayed. Click here to see all.

	Enzymes	Enzyme	Enzyme activity	EC number
[Ref.: #43103]		catalase	+	1.11.1.6
[Ref.: #43103]		cytochrome oxidase	-	1.9.3.1

Fatty acid profile

Metadata FA analysis

Reference

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Fatty acid	Percentage
C18:1ω9c	45.4
C16:0	20.1
C16:1ω6c / C16:1ω7c / C15:0 iso 2OH	14.9
C14:0	4.8
C18:0	4.4
C20:1ω9c	2.4
C16:1ω9c	1.6
C12:0	1.1
C17:1ω8c	1.1
C17:0	1

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #24393]	Sample type/isolated from	oral cavity of a healthy Chinese cobra Naja atra
[Ref.: #24393]	Host species	Naja atra
[Ref.: #24393]	Geographic location (country and/or sea, region)	Hong Kong, Queen Mary Hospital
[Ref.: #24393]	Country	China
[Ref.: #24393]	Country ISO 3 Code	CHN
[Ref.: #24393]	Continent	Asia

[Ref.: #43103]	Sample type/isolated from	oral cavities of healthy Chinese cobras (Naja atra)
[Ref.: #43103]	Geographic location (country and/or sea, region)	Hong Kong
[Ref.: #43103]	Country	China
[Ref.: #43103]	Country ISO 3 Code	CHN
[Ref.: #43103]	Continent	Asia
[Ref.: #43103]	Enrichment culture	Columbia agar (Oxoid) with 5 % defibrinated sheep blood
[Ref.: #43103]	Enrichment culture duration	2 days
[Ref.: #43103]	Enrichment culture temperature	25 ̊C

[Ref.: #67770]	Sample type/isolated from	Oral cavity of two Chinese cobras (Naja atra)
[Ref.: #67770]	Host species	Naja atra
[Ref.: #67770]	Geographic location (country and/or sea, region)	Hong Kong
[Ref.: #67770]	Country	China
[Ref.: #67770]	Country ISO 3 Code	CHN
[Ref.: #67770]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Host	#Reptilia	-
#Host Body-Site	#Oral cavity and airways	-

What are isolation sources categories?

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Global distribution of 16S sequence KT581020 (>99% sequence identity) for Tsukamurella serpentis from Microbeatlas

Aquatic Samples	78
Soil Samples	60
Animal Samples	118
Plant Samples	41
Total Samples	297

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

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Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #24393]	Tsukamurella serpentis strain HKU54 16S ribosomal RNA gene, partial sequence	KT581020	1407	ENA	1750603 tax ID

[Ref.: #43103]	GC-content	69.2 mol%
[Ref.: #24393]	GC-content	69.2 mol%	thermal denaturation, midpoint method (Tm)
[Ref.: #67770]	GC-content	66.7-71.7 mol%	thermal denaturation, midpoint method (Tm)

Availability in culture collections External links

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Culture collection no.

DSM 100915, JCM 31017

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Tsukamurella serpentis sp. nov., isolated from the oral cavity of Chinese cobras (Naja atra).	Tang Y, Teng JLL, Cheung CLW, Ngan AHY, Huang Y, Wong SSY, Yip EKT, Ng KHL, Que TL, Lau SKP, Woo PCY	Int J Syst Evol Microbiol	10.1099/ijsem.0.001187	2016	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#24393

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 100915

#43103

Ying Tang, Jade L. L. Teng, Candy L. W. Cheung, Antonio H. Y. Ngan, Yi Huang, Samson S. Y. Wong, Eric K. T. Yip, Kenneth H. L. Ng, Tak-Lun Que, Susanna K. P. Lau, Patrick C. Y. Woo: Tsukamurella serpentis sp. nov., isolated from the oral cavity of Chinese cobras (Naja atra). IJSEM 66: 3329 - 3336 2016 ( DOI 10.1099/ijsem.0.001187 , PubMed 27257031 )

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

* These data were automatically processed and therefore are not curated

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