Tsukamurella serpentis (DSM 100915, JCM 31017, HKU54)

Name: Tsukamurella serpentis (DSM 100915, JCM 31017, HKU54)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 132604

Type strain

Strain Designation HKU54

Culture col. no. DSM 100915 JCM 31017 HKU54

NCBI tax ID(s) 1750603

Special Collections DSMZ JCM Literature strains

History <- J. L. L. Teng, Univ. of Hong Kong; HKU54 J. L. L. Teng; Dept. of Microbiol., Univ. of Hong Kong, China; HKU54.

Links

Tsukamurella serpentis HKU54 is an aerobe, Gram-positive, rod-shaped prokaryote that forms irregular colonies and was isolated from oral cavity of a healthy Chinese cobra Naja atra.

Gram-positive rod-shaped colony-forming aerobe 16S sequence

Name and taxonomic classification

SI-ID 407697

JCM 31017,DSM 100915

@ref 20215

Last LPSN update 2026-02-09 11:31:45

Domain Bacteria

Phylum Actinomycetota

Class Actinomycetes

Order Mycobacteriales

Family Tsukamurellaceae

Genus Tsukamurella

Species Tsukamurella serpentis

Full scientific name Tsukamurella serpentis Tang et al. 2016

BacDive ID	Other strains from **Tsukamurella serpentis** (1)	Type strain
158213	T. serpentis HKU55, DSM 100916, JCM 31018

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Motility
43103	positive	rod-shaped

Colony morphology

@ref	Colony size	Colony color	Colony shape	Incubation period	Medium used
43103	2 mm	cream-to-yellow	irregular	2 days	Columbia agar with 5 % defibrinated sheep blood agar

Multimedia

@ref:	24393
multimedia content:	DSM_100915.jpg
multimedia.multimedia content:	https://www.dsmz.de/microorganisms/photos/DSM_100915.jpg
caption:	Medium 693 25°C
intellectual property rights:	© Leibniz-Institut DSMZ
manual_annotation:	1

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
43103	Columbia agar with 5 % defibrinated sheep blood agar
43103	brain-heart infusion agar
43103	nutritient agar (NA)
43103	trypticase soy agar (TSA)
43103	chocolate agar
43103	MacConkey agar
24393	TRYPTICASE SOY BROTH AGAR+BLOOD (DSMZ Medium 535a)	Medium recipe at MediaDive	Name: TRYPTICASE SOY BROTH AGAR+BLOOD (DSMZ Medium 535a) Composition: None 50.0 g/l Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
24393	positive	growth	25	mesophilic
43103	negative	growth	42
43103	positive	growth	10-37
67770	positive	growth	28	mesophilic

Physiology and metabolism

Oxygen tolerance

43103

Oxygen toleranceaerobe

Spore formation

43103

Spore formationno

Halophily

@ref	Salt	Growth	Tested relation	Concentration
43103	NaCl	positive	maximum	7 %(w/v)

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
43103	16808 ChEBI	2-dehydro-D-gluconate	-	assimilation
43103	58143 ChEBI	5-dehydro-D-gluconate	-	assimilation
43103	27613 ChEBI	amygdalin	-	assimilation
43103	18305 ChEBI	arbutin	-	assimilation
43103	17057 ChEBI	cellobiose	-	assimilation
43103	17108 ChEBI	D-arabinose	-	assimilation
43103	18333 ChEBI	D-arabitol	+	assimilation
43103	15824 ChEBI	D-fructose	+	assimilation
43103	28847 ChEBI	D-fucose	-	assimilation
43103	12936 ChEBI	D-galactose	+	assimilation
43103	17634 ChEBI	D-glucose	+	assimilation
43103	62318 ChEBI	D-lyxose	-	assimilation
43103	16899 ChEBI	D-mannitol	+	assimilation
43103	16988 ChEBI	D-ribose	-	assimilation
43103	17924 ChEBI	D-sorbitol	+	assimilation
43103	16443 ChEBI	D-tagatose	-	assimilation
43103	65327 ChEBI	D-xylose	-	assimilation
43103		esculin ferric citrate	-	assimilation
43103	16813 ChEBI	galactitol	-	assimilation
43103	28066 ChEBI	gentiobiose	-	assimilation
43103	28087 ChEBI	glycogen	-	assimilation
43103	15443 ChEBI	inulin	-	assimilation
43103	30849 ChEBI	L-arabinose	-	assimilation
43103	18403 ChEBI	L-arabitol	-	assimilation
43103	18287 ChEBI	L-fucose	-	assimilation
43103	62345 ChEBI	L-rhamnose	-	assimilation
43103	17266 ChEBI	L-sorbose	-	assimilation
43103	65328 ChEBI	L-xylose	-	assimilation
43103	17716 ChEBI	lactose	-	assimilation
43103	17306 ChEBI	maltose	+	assimilation
43103	6731 ChEBI	melezitose	-	assimilation
43103	28053 ChEBI	melibiose	-	assimilation
43103	43943 ChEBI	methyl alpha-D-mannoside	-	assimilation
43103	37657 ChEBI	methyl D-glucoside	-	assimilation
43103	17268 ChEBI	myo-inositol	+	assimilation
43103	506227 ChEBI	N-acetylglucosamine	-	assimilation
43103	32032 ChEBI	potassium gluconate	-	assimilation
43103	16634 ChEBI	raffinose	-	assimilation
43103	15963 ChEBI	ribitol	-	assimilation
43103	17814 ChEBI	salicin	-	assimilation
43103	28017 ChEBI	starch	-	assimilation
43103	17992 ChEBI	sucrose	+	assimilation
43103	27082 ChEBI	trehalose	+	assimilation
43103	32528 ChEBI	turanose	+	assimilation
43103	18186 ChEBI	tyrosine	-	hydrolysis
43103	15318 ChEBI	xanthine	-	hydrolysis
43103	17151 ChEBI	xylitol	+	assimilation

Enzymes

@ref	Value	Activity	Ec
43103	catalase	+	1.11.1.6
43103	cytochrome oxidase	-	1.9.3.1

Fatty acid profile

Metadata FA analysis

@ref

43103

Fatty acid	Percentage
C18:1ω9c	45.4
C16:0	20.1
C16:1ω6c / C16:1ω7c / C15:0 iso 2OH	14.9
C14:0	4.8
C18:0	4.4
C20:1ω9c	2.4
C16:1ω9c	1.6
C12:0	1.1
C17:1ω8c	1.1
C17:0	1

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2
#Host	#Reptilia
#Host Body-Site	#Oral cavity and airways

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent	Enrichment culture	Enrichment culture duration	Enrichment culture temperature	Host species
24393	oral cavity of a healthy Chinese cobra Naja atra	Hong Kong, Queen Mary Hospital	China	CHN	Asia				Naja atra
43103	oral cavities of healthy Chinese cobras (Naja atra)	Hong Kong	China	CHN	Asia	Columbia agar (Oxoid) with 5 % defibrinated sheep blood	2 days	25
67770	Oral cavity of two Chinese cobras (Naja atra)	Hong Kong	China	CHN	Asia				Naja atra

Taxonmaps

69479

Global distribution of 16S sequence KT581020 (>99% sequence identity) for Tsukamurella serpentis from Microbeatlas

Aquatic Samples	78
Soil Samples	60
Animal Samples	118
Plant Samples	41
Total Samples	297

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
24393	1	Risk group (German classification)

Sequence information

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
24393	Tsukamurella serpentis strain HKU54 16S ribosomal RNA gene, partial sequence	KT581020	1407		1750603
124043	Tsukamurella serpentis strain JCM 31017 16S ribosomal RNA gene, partial sequence.	KX924572	1221		1750603

GC content

@ref	GC-content (mol%)	Method
43103	69.2
24393	69.2	thermal denaturation, midpoint method (Tm)
67770	66.7-71.7	thermal denaturation, midpoint method (Tm)

Genome-based predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Tsukamurella serpentis sp. nov., isolated from the oral cavity of Chinese cobras (Naja atra).	Tang Y, Teng JLL, Cheung CLW, Ngan AHY, Huang Y, Wong SSY, Yip EKT, Ng KHL, Que TL, Lau SKP, Woo PCY	Int J Syst Evol Microbiol	10.1099/ijsem.0.001187	2016

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#24393	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 100915
#43103	Ying Tang, Jade L. L. Teng, Candy L. W. Cheung, Antonio H. Y. Ngan, Yi Huang, Samson S. Y. Wong, Eric K. T. Yip, Kenneth H. L. Ng, Tak-Lun Que, Susanna K. P. Lau, Patrick C. Y. Woo: Tsukamurella serpentis sp. nov., isolated from the oral cavity of Chinese cobras (Naja atra). IJSEM 66: 3329 - 3336 2016 ( DOI 10.1099/ijsem.0.001187 , PubMed 27257031 )
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Tsukamurella serpentis (DSM 100915, JCM 31017, HKU54)

Name and taxonomic classification