Anaerocolumna cellulosilytica (DSM 100423, JCM 30819, SN 021)

Name: Anaerocolumna cellulosilytica (DSM 100423, JCM 30819, SN 021)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 132584

Type strain

Culture col. no. DSM 100423 JCM 30819 SN 021

NCBI tax ID(s) 433286

Special Collections DSMZ JCM Literature strains

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Anaerocolumna cellulosilytica DSM 100423 is an obligate anaerobe, chemoorganotroph, spore-forming prokaryote that was isolated from decomposed rice straw in a methanogenic reactor treating waste from cattle farms.

spore-forming Gram-positive motile rod-shaped obligate anaerobe chemoorganotroph mesophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 402717

JCM 30819,DSM 100423

@ref 20215

Last LPSN update 2025-12-16 10:01:33

Domain Bacillati

Phylum Bacillota

Class Clostridia

Order Eubacteriales

Family Lachnospiraceae

Genus Anaerocolumna

Species Anaerocolumna cellulosilytica

Full scientific name Anaerocolumna cellulosilytica Ueki et al. 2016

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape	Motility	Flagellum arrangement
43082	positive	9.5-12.0 µm	0.8 µm	rod-shaped		peritrichous

Colony morphology

@ref	Colony color	Medium used
43082	translucent	PY4S agar

Culture and growth conditions

Culture medium

@ref	Name	Composition
43082	PY4S agar	peptone/yeast extract (PY) basal medium supplemented with glucose, cellobiose, maltose and soluble starch
43082	PYG
24373	WILKINS-CHALGREN ANAEROBE BROTH (DSMZ Medium 339)	Name: WILKINS-CHALGREN ANAEROBE BROTH (DSMZ Medium 339) Composition: dehydrated Wilkins-Chalgren medium 33.0 g/l L-Cysteine HCl 0.3 g/l Sodium resazurin 0.0005 g/l Distilled water
43082	chopped meat broth

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
24373	positive	growth	35	mesophilic
43082	positive	optimum	35	mesophilic
43082	positive	growth	15-40
67770	positive	growth	30	mesophilic

Culture pH

@ref	Ability	Type	PH
43082	positive	optimum	7.6
43082	positive	growth	6.2-8.5

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance
43082	obligate anaerobe
125439	facultative anaerobe

Nutrition type

43082

Typechemoorganotroph

Spore formation

@ref	Spore description	Spore formation
43082	terminal, spherical spores from swollen cells

Halophily

@ref	Salt	Growth	Tested relation	Concentration
43082	NaCl	positive	growth	0-30 g/L
43082	NaCl	positive	optimum	0 g/L

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
43082	28816 ChEBI	2-deoxy-d-ribose	-	assimilation
43082	27613 ChEBI	amygdalin	+	assimilation
43082	22599 ChEBI	arabinose	+	assimilation
43082	85146 ChEBI	carboxymethylcellulose	+	assimilation
43082	17057 ChEBI	cellobiose	+	assimilation
43082	62968 ChEBI	cellulose	+	assimilation
43082	23652 ChEBI	dextrin	+	assimilation
43082	4853 ChEBI	esculin	+	assimilation
43082	4853 ChEBI	esculin	+	hydrolysis
43082	28757 ChEBI	fructose	+	assimilation
43082	29806 ChEBI	fumarate	-	assimilation
43082	16813 ChEBI	galactitol	-	assimilation
43082	28260 ChEBI	galactose	+	assimilation
43082	5291 ChEBI	gelatin	-	hydrolysis
43082	17234 ChEBI	glucose	+	assimilation
43082	17754 ChEBI	glycerol	-	assimilation
43082	28087 ChEBI	glycogen	+	assimilation
43082	15443 ChEBI	inulin	-	assimilation
43082	24996 ChEBI	lactate	-	assimilation
43082	17716 ChEBI	lactose	+	assimilation
43082	25115 ChEBI	malate	-	assimilation
43082	17306 ChEBI	maltose	+	assimilation
43082	29864 ChEBI	mannitol	-	assimilation
43082	37684 ChEBI	mannose	+	assimilation
43082	6731 ChEBI	melezitose	-	assimilation
43082	28053 ChEBI	melibiose	+	assimilation
43082		milk	+	hydrolysis
43082	17268 ChEBI	myo-inositol	-	assimilation
43082	17632 ChEBI	nitrate	-	reduction
43082	17309 ChEBI	pectin	+	assimilation
43082	15361 ChEBI	pyruvate	-	assimilation
43082	16634 ChEBI	raffinose	-	assimilation
43082	26546 ChEBI	rhamnose	-	assimilation
43082	17814 ChEBI	salicin	+	assimilation
43082	30911 ChEBI	sorbitol	-	assimilation
43082	27922 ChEBI	sorbose	-	assimilation
43082	28017 ChEBI	starch	+	assimilation
43082	28017 ChEBI	starch	+	hydrolysis
43082	30031 ChEBI	succinate	-	assimilation
43082	17992 ChEBI	sucrose	-	assimilation
43082	27082 ChEBI	trehalose	-	assimilation
43082	37166 ChEBI	xylan	+	assimilation
43082	18222 ChEBI	xylose	+	assimilation

Metabolite production

@ref	Chebi-ID	Metabolite
43082	30089 ChEBI	acetate
43082	16526 ChEBI	carbon dioxide
43082	18276 ChEBI	dihydrogen
43082	16236 ChEBI	ethanol
43082	16136 ChEBI	hydrogen sulfide
43082	35581 ChEBI	indole
43082	24996 ChEBI	lactate
43082	30031 ChEBI	succinate

Enzymes

@ref	Value	Activity	Ec
43082	catalase	-	1.11.1.6
43082	cytochrome oxidase	-	1.9.3.1
43082	lecithinase	-
43082	lipase	-

Fatty acid profile

Metadata FA analysis

@ref

43082

Fatty acid	Percentage	ECL
C16:0 ALDE	20.6
C16:0 DMA	17.4
C17:1ω8c / C17:2	12.3
C18:1ω7c DMA	9
Unknown 14.762 / C15:2	5.6
C16:0	5.5
C17:2 / C17:1ω9c	4.2	16758
C16:1ω7c DMA	3.8
C18:1ω9c DMA	3.1
C14:0 DMA	1.8
C19:0 cyclo 11,12 DMA	1.4
C16:0 3OH	1.2
C14:0	0.6

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2
#Engineered	#Bioreactor
#Engineered	#Waste
#Host	#Plants
#Host	#Plants

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
24373	decomposed rice straw in a methanogenic reactor treating waste from cattle farms	Hokkaido, Betsukai-machi	Japan	JPN	Asia
43082	methanogenic reactor treating waste from cattle farms (samples of small pieces of decomposed rice straw)	Betsukai-machi, Hokkaido	Japan	JPN	Asia
67770	Methanogenic reactor		Japan	JPN	Asia

Taxonmaps

69479

Global distribution of 16S sequence AB298755 (>99% sequence identity) for Anaerocolumna cellulosilytica subclade from Microbeatlas

Aquatic Samples	89
Soil Samples	436
Animal Samples	199
Plant Samples	428
Total Samples	1152

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
24373	1	Risk group (German classification)

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM1420287v1 assembly for Anaerocolumna cellulosilytica DSM 100423	scaffold	GCA_014202875	433286.3	2861627031	433286	64.75

Genome browser: GCA_014202875

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
24373	Anaerocolumna cellulosilytica gene for 16S rRNA, partial sequence	AB298755	1478		433286

GC content

@ref	GC-content (mol%)	Method
24373	38.1	high performance liquid chromatography (HPLC)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Anaerocolumna cellulosilytica DSM 100423
NCBI: GCA_014202875

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	yes	87.20	no
125439	motility	BacteriaNetⓘ	yes	87.50	no
125439	gram_stain	BacteriaNetⓘ	variable	81.20	no
125439	oxygen_tolerance	BacteriaNetⓘ	facultative anaerobe	96.70	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Anaerocolumna cellulosilytica DSM 100423
NCBI: GCA_014202875.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	73.11	yes
125438	anaerobic	anaerobicⓘ	yes	85.79	yes
125438	aerobic	aerobicⓘ	no	91.11	yes
125438	spore-forming	spore-formingⓘ	yes	81.37	yes
125438	thermophilic	thermophileⓘ	no	94.62	yes
125438	flagellated	motile2+ⓘ	yes	79.10	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Descriptions of Anaerotaenia torta gen. nov., sp. nov. and Anaerocolumna cellulosilytica gen. nov., sp. nov. isolated from a methanogenic reactor of cattle waste and reclassification of Clostridium aminovalericum, Clostridium jejuense and Clostridium xylanovorans as Anaerocolumna species.	Ueki A, Ohtaki Y, Kaku N, Ueki K	Int J Syst Evol Microbiol	10.1099/ijsem.0.001123	2016
Phylogeny	Anaerocolumna sedimenticola sp. nov., isolated from fresh water sediment.	Kim J, Kim JY, Song HS, Kim YB, Whon TW, Ahn SW, Lee SH, Yoo S, Kim YJ, Myoung J, Choi YE, Son HS, Roh SW	Antonie Van Leeuwenhoek	10.1007/s10482-021-01536-4	2021

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#24373	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 100423
#43082	Atsuko Ueki, Yoshimi Ohtaki, Nobuo Kaku, Katsuji Ueki: Descriptions of Anaerotaenia torta gen. nov., sp. nov. and Anaerocolumna cellulosilytica gen. nov., sp. nov. isolated from a methanogenic reactor of cattle waste and reclassification of Clostridium aminovalericum, Clostridium jejuense and Clostridium xylanovorans as Anaerocolumna species. IJSEM 66: 2936 - 2943 2016 ( DOI 10.1099/ijsem.0.001123 , PubMed 27126251 )
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Anaerocolumna cellulosilytica (DSM 100423, JCM 30819, SN 021)

Name and taxonomic classification