Name: Anaerotaenia torta FH052
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 132583

Type strain:

Species: Anaerotaenia torta

Strain Designation: FH052

Culture col. no.: DSM 100431, JCM 30820

Strain history: A. Ueki; Fac. of Agric., Yamagata Univ., Japan; FH052.

NCBI tax ID(s): 433293 (species)

SI-ID 400259

DSM 100431

Section

Anaerotaenia torta FH052 is an obligate anaerobe, chemoorganotroph, spore-forming bacterium that was isolated from digester fluid without plant residue or other solid material from a methanogenic reactor treating waste from cattle farms.

Gram-positive
motile
rod-shaped
spore-forming
chemoorganotroph
obligate anaerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Clostridia*
	Order	*Eubacteriales*
	Family	*Lachnospiraceae*
	Genus	*Anaerotaenia*
	Species	**Anaerotaenia torta**
	Full Scientific Name (LPSN)	Anaerotaenia torta Ueki et al. 2016

Information on morphological and physiological properties Morphology

[Ref.: #43082]	Gram stain	positive

[Ref.: #43082]	Cell length	6.4-9 µm

[Ref.: #43082]	Cell width	0.4-0.5 µm

[Ref.: #43082]	Cell shape	rod-shaped

[Ref.: #43082]	Motility	yes

[Ref.: #43082]	Flagellum arrangement	peritrichous

[Ref.: #43082]	Colony color	translucent
[Ref.: #43082]	Cultivation medium used	PY4S agar

Information on culture and growth conditions Culture and growth conditions

[Ref.: #43082]

Culture medium

PY4S agar

[Ref.: #43082]

Culture medium growth

[Ref.: #43082]

Culture medium composition

expand / minimize

[Ref.: #43082]

Culture medium

PYG medium

[Ref.: #43082]

Culture medium growth

[Ref.: #24372]

Culture medium

PY + X MEDIUM (DSMZ Medium 104b)

[Ref.: #24372]

Culture medium growth

[Ref.: #24372]

Culture medium link

Medium recipe at MediaDive

[Ref.: #24372]

Culture medium composition

expand / minimize

[Ref.: #43082]

Culture medium

chopped meat broth

[Ref.: #43082]

Culture medium growth

		Temperature
[Ref.: #24372]	growth	35 °C
[Ref.: #43082]	optimum	35-40 °C
[Ref.: #43082]	growth	20-40 °C
[Ref.: #67770]	growth	30 °C

[Ref.: #24372]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #43082]			optimum	7.4
[Ref.: #43082]			growth	6.8-8.2

Information on physiology and metabolism Physiology and metabolism

[Ref.: #43082]

Oxygen tolerance

obligate anaerobe

[Ref.: #43082]

Nutrition type

chemoorganotroph

[Ref.: #43082]	Spore description	terminal, spherical spores
[Ref.: #43082]	Ability of spore formation	yes

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #43082]		NaCl		growth	0-10	g/L
[Ref.: #43082]		NaCl		optimum	0	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #43082]	amygdalin ChEBI	-	assimilation
[Ref.: #43082]	arabinose ChEBI	+	assimilation
[Ref.: #43082]	carboxymethylcellulose ChEBI	-	assimilation
[Ref.: #43082]	cellobiose ChEBI	+	assimilation
[Ref.: #43082]	cellulose ChEBI	-	assimilation
[Ref.: #43082]	dextrin ChEBI	-	assimilation
[Ref.: #43082]	esculin ChEBI	-	assimilation
[Ref.: #43082]	esculin ChEBI	-	hydrolysis
[Ref.: #43082]	fructose ChEBI	+	assimilation
[Ref.: #43082]	fumarate ChEBI	-	assimilation
[Ref.: #43082]	galactitol ChEBI	-	assimilation
[Ref.: #43082]	galactose ChEBI	+	assimilation
[Ref.: #43082]	gelatin ChEBI	-	hydrolysis
[Ref.: #43082]	glucose ChEBI	+	assimilation
[Ref.: #43082]	glycerol ChEBI	-	assimilation
[Ref.: #43082]	glycogen ChEBI	-	assimilation
[Ref.: #43082]	inulin ChEBI	-	assimilation
[Ref.: #43082]	lactate ChEBI	-	assimilation
[Ref.: #43082]	lactose ChEBI	-	assimilation
[Ref.: #43082]	malate ChEBI	-	assimilation
[Ref.: #43082]	maltose ChEBI	-	assimilation
[Ref.: #43082]	mannitol ChEBI	+	assimilation
[Ref.: #43082]	mannose ChEBI	+	assimilation
[Ref.: #43082]	melezitose ChEBI	-	assimilation
[Ref.: #43082]	melibiose ChEBI	-	assimilation
[Ref.: #43082]	milk	-	hydrolysis
[Ref.: #43082]	myo-inositol ChEBI	-	assimilation
[Ref.: #43082]	nitrate ChEBI	-	reduction
[Ref.: #43082]	pectin ChEBI	-	assimilation
[Ref.: #43082]	pyruvate ChEBI	+	assimilation
[Ref.: #43082]	raffinose ChEBI	-	assimilation
[Ref.: #43082]	rhamnose ChEBI	+	assimilation
[Ref.: #43082]	ribose ChEBI	+	assimilation
[Ref.: #43082]	salicin ChEBI	+	assimilation
[Ref.: #43082]	sorbitol ChEBI	-	assimilation
[Ref.: #43082]	sorbose ChEBI	-	assimilation
[Ref.: #43082]	starch ChEBI	-	assimilation
[Ref.: #43082]	starch ChEBI	-	hydrolysis
[Ref.: #43082]	succinate ChEBI	-	assimilation
[Ref.: #43082]	sucrose ChEBI	+	assimilation
[Ref.: #43082]	trehalose ChEBI	-	assimilation
[Ref.: #43082]	xylan ChEBI	+	assimilation
[Ref.: #43082]	xylose ChEBI	+	assimilation
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Production
[Ref.: #43082]	acetate ChEBI	yes
[Ref.: #43082]	carbon dioxide ChEBI	yes
[Ref.: #43082]	dihydrogen ChEBI	yes
[Ref.: #43082]	ethanol ChEBI	yes
[Ref.: #43082]	hydrogen sulfide ChEBI	no
[Ref.: #43082]	indole ChEBI	no
[Ref.: #43082]	lactate ChEBI	no
[Ref.: #43082]	succinate ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #43082]	catalase	-	1.11.1.6
[Ref.: #43082]	cytochrome oxidase	-	1.9.3.1
[Ref.: #43082]	lecithinase	-
[Ref.: #43082]	lipase	-

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #43082]

Fatty acid	Percentage	ECL
C16:0	30.8
C18:1ω7c DMA	19
C18:1ω7c / Unknown 17.834	10.6	17823
C16:0 DMA	8.7
C17:1ω8c / C17:2	5	16799
C16:1ω7c DMA	4.8
C16:0 3OH	2.9
C14:0	2.1
C16:0 ALDE	2.1
Unknown 14.762 / C15:2	1.8	14778
C14:0 DMA	1.8
C18:1ω9c DMA	1
C19:0 cyclo 11,12 DMA	1

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #24372]	Sample type/isolated from	digester fluid without plant residue or other solid material from a methanogenic reactor treating waste from cattle farms
[Ref.: #24372]	Geographic location (country and/or sea, region)	Hokkaido, Betsukai-machi
[Ref.: #24372]	Country	Japan
[Ref.: #24372]	Country ISO 3 Code	JPN
[Ref.: #24372]	Continent	Asia

[Ref.: #43082]	Sample type/isolated from	methanogenic reactor treating waste from cattle farms (digester fluid)
[Ref.: #43082]	Geographic location (country and/or sea, region)	Betsukai-machi, Hokkaido
[Ref.: #43082]	Country	Japan
[Ref.: #43082]	Country ISO 3 Code	JPN
[Ref.: #43082]	Continent	Asia
[Ref.: #43082]	Enrichment culture	peptone/yeast extract (PY) basal medium
[Ref.: #43082]	Enrichment culture composition	10 g/l trypticase, 5 g/l yeast extract, 0.2 g Na2CO3, 0.3 g/l L-cysteine hydrochloride dihydrate, 1 mg/l sodium resazurin, salt solutions
[Ref.: #43082]	Enrichment culture temperature	30 ̊C
[Ref.: #43082]	Isolation/enrichment procedure	anaerobic roll-tube method

[Ref.: #67770]	Sample type/isolated from	Methanogenic reactor
[Ref.: #67770]	Country	Japan
[Ref.: #67770]	Country ISO 3 Code	JPN
[Ref.: #67770]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Engineered	#Agriculture	#Livestock (Husbandry)
#Engineered	#Bioreactor	-
#Host	#Mammals	#Bovinae (Cow, Cattle)
#Host Body Product	#Gastrointestinal tract	#Feces (Stool)

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AB298768 (>99% sequence identity) for Anaerotaenia torta subclade from Microbeatlas

Aquatic Samples	12
Soil Samples	4
Animal Samples	18
Plant Samples	1
Total Samples	35

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #24372]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #24372]	Anaerotaenia torta gene for 16S rRNA, partial sequence	AB298768	1479	ENA	433293 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Anaerotaenia torta DSM 100431	2861347227	draft	JGI IMG/M	433293 tax ID	*

[Ref.: #24372]

GC-content

47.2 mol%

high performance liquid chromatography (HPLC)

Availability in culture collections External links

[Ref.: #24372]

Culture collection no.

DSM 100431, JCM 30820

[Ref.: #90900]

SI-ID 400259

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Descriptions of Anaerotaenia torta gen. nov., sp. nov. and Anaerocolumna cellulosilytica gen. nov., sp. nov. isolated from a methanogenic reactor of cattle waste and reclassification of Clostridium aminovalericum, Clostridium jejuense and Clostridium xylanovorans as Anaerocolumna species.	Ueki A, Ohtaki Y, Kaku N, Ueki K	Int J Syst Evol Microbiol	10.1099/ijsem.0.001123	2016	*
Phylogeny	Variimorphobacter saccharofermentans gen. nov., sp. nov., a new member of the family Lachnospiraceae, isolated from a maize-fed biogas fermenter.	Rettenmaier R, Thieme N, Streubel J, Di Bello L, Kowollik ML, Huang L, Maus I, Klingl A, Liebl W, Zverlov VV	Int J Syst Evol Microbiol	10.1099/ijsem.0.005044	2021	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#24372

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 100431

#43082

Atsuko Ueki, Yoshimi Ohtaki, Nobuo Kaku, Katsuji Ueki: Descriptions of Anaerotaenia torta gen. nov., sp. nov. and Anaerocolumna cellulosilytica gen. nov., sp. nov. isolated from a methanogenic reactor of cattle waste and reclassification of Clostridium aminovalericum, Clostridium jejuense and Clostridium xylanovorans as Anaerocolumna species. IJSEM 66: 2936 - 2943 2016 ( DOI 10.1099/ijsem.0.001123 , PubMed 27126251 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#90900

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID400259.1 )

* These data were automatically processed and therefore are not curated

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