Rothia aerolata (DSM 102816, CCM 8669, LMG 29446)

Name: Rothia aerolata (DSM 102816, CCM 8669, LMG 29446)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 132581

Type strain

Strain Designation 140917-MRSA-09

Culture col. no. DSM 102816 CCM 8669 LMG 29446 140917-MRSA-09 JCM 31759 1 more

NCBI tax ID(s) 1812262

Special Collections DSMZ JCM Literature strains CIP

History <- P. Kämpfer, Justus-Liebig-Universität, Giessen, Germany; 140917-MRSA-09 CCM 8669 <-- P. Kämpfer; Justus-Liebig-Univ. Giessen, Germany; 140917-MRSA-09. CIP <- 2016, P. Kämpfer, Giessen Univ., Giessen, Germany: strain 140917-MRSA-09

Links

Rothia aerolata 140917-MRSA-09 is an aerobe, Gram-positive, rod-shaped bacterium that was isolated from exhaust air of a pig barn .

Gram-positive rod-shaped aerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 413549

LMG 29446,CIP 111029

@ref 20215

Last LPSN update 2026-02-09 11:31:45

Domain Bacteria

Phylum Actinomycetota

Class Actinomycetes

Order Micrococcales

Family Micrococcaceae

Genus Rothia

Species Rothia aerolata

Full scientific name Rothia aerolata Kämpfer et al. 2016

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Confidence
43071	positive	rod-shaped
43071		coccus-shaped
125438			94
125438	positive		92.401
116070	positive	oval-shaped

Colony morphology

@ref	Colony size	Colony color	Medium used
43071	1-2 mm	creamy-whitish to beige	TSA

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
42184	MEDIUM 72- for trypto casein soja agar		Distilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
43071	Trypticase Soy Agar (TSA)
43071	Nutrient agar (NA)
43071	PYE agar
43071	blood agar
24370	TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)	Medium recipe at MediaDive	Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
116070	CIP Medium 72	Medium recipe at CIP
43071	MacConkey agar

Culture temp

@ref	Growth	Type	Temperature (°C)
24370	positive	growth	28
42184	positive	growth	30
43071	inconsistent	growth	42
43071	negative	growth	50
43071	negative	growth	10
43071	positive	growth	15-36
67770	positive	growth	28

Culture pH

@ref	Ability	Type	PH	PH range
43071	positive	growth	6.5-9.5	alkaliphile
43071	inconsistent	growth	5.5
43071		growth	4.5

Physiology and metabolism

Oxygen tolerance

43071

Oxygen toleranceaerobe

Spore formation

@ref	Type of spore	Spore formation	Confidence
43071	spore
125439			92.8

Halophily

@ref	Salt	Growth	Tested relation	Concentration
43071	NaCl	positive	growth	0-5 %(w/v)
43071	NaCl	inconsistent	growth	6-8 %
43071	NaCl		growth	9 %

Observation

67770

Observationquinones: MK-8, MK-7

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
43071		2-deoxythymidine-5'-4-nitrophenyl phosphate	+/-	hydrolysis
43071	30916 ChEBI	2-oxoglutarate	-	assimilation
43071	16193 ChEBI	3-hydroxybenzoate	-	assimilation
43071	37054 ChEBI	3-hydroxybutyrate	-	assimilation
43071	17879 ChEBI	4-hydroxybenzoate	-	assimilation
43071	91122 ChEBI	4-nitrophenyl alpha-D-glucopyranoside	+	hydrolysis
43071	90259 ChEBI	4-nitrophenyl beta-D-glucopyranoside	-	hydrolysis
43071	90146 ChEBI	4-nitrophenyl beta-D-glucuronide	-	hydrolysis
43071	90148 ChEBI	4-nitrophenyl beta-D-xylopyranoside	-	hydrolysis
43071	17440 ChEBI	4-nitrophenyl phosphate	+	hydrolysis
43071	30089 ChEBI	acetate	+	assimilation
43071	17128 ChEBI	adipate	-	assimilation
43071	27907 ChEBI	alpha-L-rhamnose	-	assimilation
43071	18305 ChEBI	arbutin	-	assimilation
43071	78208 ChEBI	azelaate	-	assimilation
43071	16958 ChEBI	beta-alanine	+	assimilation
43071	3122 ChEBI	bis-4-nitrophenyl phosphate	+	hydrolysis
43071	17057 ChEBI	cellobiose	-	assimilation
43071	17057 ChEBI	cellobiose	-	builds acid from
43071	16383 ChEBI	cis-aconitate	-	assimilation
43071	16947 ChEBI	citrate	-	assimilation
43071	18333 ChEBI	D-arabitol	-	builds acid from
43071	15824 ChEBI	D-fructose	+	assimilation
43071	12936 ChEBI	D-galactose	+	assimilation
43071	8391 ChEBI	D-gluconate	-	assimilation
43071	17634 ChEBI	D-glucose	+	builds acid from
43071	17634 ChEBI	D-glucose	+	assimilation
43071	16899 ChEBI	D-mannitol	-	assimilation
43071	16899 ChEBI	D-mannitol	-	builds acid from
43071	16024 ChEBI	D-mannose	+	assimilation
43071	16988 ChEBI	D-ribose	+	assimilation
43071	17924 ChEBI	D-sorbitol	-	assimilation
43071	65327 ChEBI	D-xylose	-	assimilation
43071	65327 ChEBI	D-xylose	+	builds acid from
43071	17113 ChEBI	erythritol	-	builds acid from
43071	4853 ChEBI	esculin	+	hydrolysis
43071	29806 ChEBI	fumarate	+	assimilation
43071	16813 ChEBI	galactitol	-	builds acid from
43071	16865 ChEBI	gamma-aminobutyric acid	-	assimilation
43071	17234 ChEBI	glucose	+	builds acid from
43071	30921 ChEBI	glutarate	-	assimilation
43071	28087 ChEBI	glycogen	-	builds acid from
43071	17240 ChEBI	itaconate	-	assimilation
43071	16977 ChEBI	L-alanine	-	assimilation
43071	30849 ChEBI	L-arabinose	+	assimilation
43071	30849 ChEBI	L-arabinose	+	builds acid from
43071	29991 ChEBI	L-aspartate	+	assimilation
43071		L-glutamate-gamma-3-carboxy-4-nitroanilide	-	hydrolysis
43071	15603 ChEBI	L-leucine	-	assimilation
43071	15589 ChEBI	L-malate	+	assimilation
43071	15729 ChEBI	L-ornithine	+	assimilation
43071	17295 ChEBI	L-phenylalanine	-	assimilation
43071		L-proline-4-nitroanilide	+	assimilation
43071	17115 ChEBI	L-serine	+	assimilation
43071	16828 ChEBI	L-tryptophan	-	assimilation
43071	24996 ChEBI	lactate	+	assimilation
43071	17716 ChEBI	lactose	-	builds acid from
43071	68428 ChEBI	maltitol	+	assimilation
43071	17306 ChEBI	maltose	+	assimilation
43071	17306 ChEBI	maltose	+	builds acid from
43071	29864 ChEBI	mannitol	-	builds acid from
43071	28053 ChEBI	melibiose	+	assimilation
43071	28053 ChEBI	melibiose	-	builds acid from
43071	36986 ChEBI	mesaconate	-	assimilation
43071	17268 ChEBI	myo-inositol	-	assimilation
43071	17268 ChEBI	myo-inositol	-	builds acid from
43071	28037 ChEBI	N-acetylgalactosamine	-	assimilation
43071	506227 ChEBI	N-acetylglucosamine	-	assimilation
43071	17632 ChEBI	nitrate	+	reduction
43071		O-nitrophenyl-beta-D-galactopyranosid	-	hydrolysis
43071	55394 ChEBI	p-nitrophenylphosphocholine	-	hydrolysis
43071	18401 ChEBI	phenylacetate	-	assimilation
43071	17272 ChEBI	propionate	+	assimilation
43071	17148 ChEBI	putrescine	-	assimilation
43071	15361 ChEBI	pyruvate	+	assimilation
43071	16634 ChEBI	raffinose	-	builds acid from
43071	26546 ChEBI	rhamnose	-	builds acid from
43071	15963 ChEBI	ribitol	+	assimilation
43071	33942 ChEBI	ribose	-	builds acid from
43071	17814 ChEBI	salicin	-	assimilation
43071	17814 ChEBI	salicin	-	builds acid from
43071	30911 ChEBI	sorbitol	-	builds acid from
43071	9300 ChEBI	suberic acid	-	assimilation
43071	17992 ChEBI	sucrose	-	builds acid from
43071	17992 ChEBI	sucrose	+	assimilation
43071	17992 ChEBI	sucrose	+	builds acid from
43071	15708 ChEBI	trans-aconitate	+	assimilation
43071	27082 ChEBI	trehalose	+	assimilation
43071	27082 ChEBI	trehalose	+	builds acid from
43071	18222 ChEBI	xylose	-	builds acid from

Metabolite production

@ref	Chebi-ID	Metabolite	Production
43071	15688 ChEBI	acetoin

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test
43071	15688 ChEBI	acetoin	-

Enzymes

@ref	Value	Activity	Ec
43071	alkaline phosphatase	-	3.1.3.1
43071	alpha-galactosidase	+	3.2.1.22
43071	alpha-glucosidase	-	3.2.1.20
43071	beta-galactosidase	-	3.2.1.23
43071	beta-glucosidase	-	3.2.1.21
43071	beta-glucuronidase	-	3.2.1.31
43071	catalase	+	1.11.1.6
43071	cytochrome oxidase	-	1.9.3.1
43071	esterase (C 4)	-
43071	esterase Lipase (C 8)	-
43071	gelatinase	-
43071	N-acetyl-beta-glucosaminidase	-	3.2.1.52
43071	pyrazinamidase	+	3.5.1.B15
43071	pyrrolidonyl arylamidase	-	3.4.19.3
43071	trypsin	-	3.4.21.4
43071	urease	-	3.5.1.5
43071	valine arylamidase	-

Fatty acid profile

Metadata FA analysis

@ref

43071

Fatty acid	Percentage
C15:0 anteiso	51.2
C17:0 anteiso	26.8
C16:0 iso	10.8
C16:0	6.5
C15:0 iso	1.6
C17:0 iso	0.7
C14:0 iso	0.6
C14:0	0.6
C13:0 anteiso	0.5

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Engineered	#Agriculture	#Livestock (Husbandry)
#Environmental	#Air	-

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent	Isolation date
24370	exhaust air of a pig barn (bioaerosol sampling)	North Rhine-Westphalia	Germany	DEU	Europe
43071	exhausted air of a pig barn (bioaerosol sampling)	North-Rhine-Westphalia, Germany	Germany	DEU	Europe
67770	Exhaust air of a pig barn	North Rhine-Westphalia	Germany	DEU	Europe
116070	Environment, Air exhaust duct of pig fattening farms	North Rhein-Westphalia, Germany	Germany	DEU	Europe	2014-01-02

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
24370	1	Risk group (German classification)
116070	1	Risk group (French classification)

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM1463558v1 assembly for Rothia aerolata CCM 8669	scaffold	GCA_014635585	1812262.3	2989492736	1812262	71.42

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
24370	Rothia aerolata strain 140917-MRSA-09 16S ribosomal RNA gene, partial sequence	KU878871	1391	1812262
124043	Rothia aerolata strain CCM 8669 16S ribosomal RNA gene, partial sequence.	MT760050	1353	1812262
124043	Rothia aerolata strain CCM 8669 16S ribosomal RNA gene, partial sequence.	MT758018	1353	1812262

GC content

@ref	GC-content (mol%)	Method
43071	58.9	Thermal denaturation, fluorometry
24370	58.9	fluorimetric
67770	58.9	thermal denaturation, midpoint method (Tm)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Rothia aerolata CCM 8669
NCBI: GCA_014635585

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	92.80	no
125439	motility	BacteriaNetⓘ	no	83.60	no
125439	gram_stain	BacteriaNetⓘ	variable	69.90	no
125439	oxygen_tolerance	BacteriaNetⓘ	anaerobe	86.30	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Rothia aerolata CCM 8669
NCBI: GCA_014635585.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	92.40	no
125438	anaerobic	anaerobicⓘ	no	95.30	yes
125438	aerobic	aerobicⓘ	yes	69.70	yes
125438	spore-forming	spore-formingⓘ	no	79.68	yes
125438	thermophilic	thermophileⓘ	no	94.61	no
125438	flagellated	motile2+ⓘ	no	94.00	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Rothia aerolata sp. nov., isolated from exhaust air of a pig barn.	Kampfer P, Kleinhagauer T, Busse HJ, Klug K, Jackel U, Glaeser SP	Int J Syst Evol Microbiol	10.1099/ijsem.0.001153	2016

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#24370	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 102816
#42184	; Curators of the CIP;
#43071	Peter Kämpfer, Tanita Kleinhagauer, Hans-Jürgen Busse, Kerstin Klug, Udo Jäckel and Stefanie P. Glaeser: Rothia aerolata sp. nov., isolated from exhaust air of a pig barn. IJSEM 66: 3102 - 3107 2016 ( DOI 10.1099/ijsem.0.001153 , PubMed 27188856 )
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#116070	Collection of Institut Pasteur ; Curators of the CIP; CIP 111029
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Rothia aerolata (DSM 102816, CCM 8669, LMG 29446)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Observation

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

Fatty acid profile

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_014635585

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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