Name: Brucella vulpis F60
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 132528

Type strain:

Species: Brucella vulpis

Strain Designation: F60

Culture col. no.: DSM 101715, BCCN 09-2

Strain history: <- H. C. Scholz, Bundeswehr Institute of Microbiology, Munich, Germany <- E. Hofer <- R. Posch <- K. Reisp, Austrian Agency for Health and Food Safety; F60

NCBI tax ID(s): 981386 (species)

SI-ID 403993

DSM 101715

Section

Brucella vulpis F60 is an aerobe, mesophilic, Gram-negative bacterium that forms circular colonies and was isolated from mandibular lymph nodes of a red foxes .

coccus-shaped
colony-forming
Gram-negative
aerobe
mesophilic
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Alphaproteobacteria*
	Order	*Hyphomicrobiales*
	Family	*Brucellaceae*
	Genus	*Brucella*
	Species	**Brucella vulpis**
	Full Scientific Name (LPSN)	Brucella vulpis Scholz et al. 2016

Information on morphological and physiological properties Morphology

[Ref.: #43648]	Gram stain	negative

[Ref.: #43648]	Cell length	0.4-1.3 µm

[Ref.: #43648]	Cell width	0.5-0.7 µm

[Ref.: #43648]	Cell shape	coccus-shaped

[Ref.: #43648]	Motility	no

[Ref.: #43648]	Type of hemolysis	gamma
[Ref.: #43648]	Colony size	0.5 mm
[Ref.: #43648]	Colony color	Greyish
[Ref.: #43648]	Colony shape	circular
[Ref.: #43648]	Incubation period	3 days
[Ref.: #43648]	Cultivation medium used	Columbia Agar

Information on culture and growth conditions Culture and growth conditions

[Ref.: #43648]

Culture medium

Columbia Agar

[Ref.: #43648]

Culture medium growth

[Ref.: #24317]

Culture medium

COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)

[Ref.: #24317]

Culture medium growth

[Ref.: #24317]

Culture medium link

Medium recipe at MediaDive

[Ref.: #24317]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #24317]			growth	37 °C
[Ref.: #43648]			growth	30-37 °C

[Ref.: #24317]	Temperature range	mesophilic
[Ref.: #43648]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #43648]

Oxygen tolerance

aerobe

[Ref.: #43648]

Ability of spore formation

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #43648]	(-)-D-fructose ChEBI	+	assimilation
[Ref.: #43648]	4-nitrophenyl alpha-D-glucopyranoside ChEBI	-	assimilation
[Ref.: #43648]	4-nitrophenyl beta-D-xylopyranoside ChEBI	-	assimilation
[Ref.: #43648]	4-nitrophenyl N-acetyl-beta-D-glucosaminide ChEBI	+	assimilation
[Ref.: #43648]	4-nitrophenyl phosphate ChEBI	-	assimilation
[Ref.: #43648]	6-deoxy-d-galactose ChEBI	+/-	assimilation
[Ref.: #43648]	acetate ChEBI	+/-	assimilation
[Ref.: #43648]	adenine ChEBI	+/-	assimilation
[Ref.: #43648]	adipate ChEBI	-	assimilation
[Ref.: #68369]	adipate ChEBI	-	assimilation	* from API 20NE
[Ref.: #68381]	alpha-cyclodextrin ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68381]	arginine ChEBI	+	hydrolysis	* from API rID32STR
[Ref.: #68369]	arginine ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #43648]	asparagine ChEBI	-	assimilation
[Ref.: #43648]	beta-hydroxybutyrate ChEBI	-	assimilation
[Ref.: #43648]	bis-4-nitrophenyl phosphate ChEBI	-	assimilation
[Ref.: #43648]	D-alanine ChEBI	-	assimilation
[Ref.: #43648]	D-arabinose ChEBI	-	assimilation
[Ref.: #68381]	D-arabitol ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #43648]	D-galactose ChEBI	-	assimilation
[Ref.: #43648]	D-glucose ChEBI	-	assimilation
[Ref.: #68369]	D-glucose ChEBI	-	fermentation	* from API 20NE
[Ref.: #43648]	D-histidine ChEBI	-	assimilation
[Ref.: #68369]	D-mannitol ChEBI	-	assimilation	* from API 20NE
[Ref.: #68381]	D-mannitol ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #43648]	D-proline ChEBI	-	assimilation
[Ref.: #43648]	D-ribose ChEBI	-	assimilation
[Ref.: #68381]	D-ribose ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #43648]	D-serine ChEBI	+/-	assimilation
[Ref.: #68381]	D-tagatose ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #43648]	D-xylose ChEBI	-	assimilation
[Ref.: #68369]	decanoate ChEBI	-	assimilation	* from API 20NE
[Ref.: #43648]	erythritol ChEBI	+/-	assimilation
[Ref.: #68369]	esculin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #43648]	fumarate ChEBI	-	assimilation
[Ref.: #68369]	gelatin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	gluconate ChEBI	-	assimilation	* from API 20NE
[Ref.: #43648]	glutaric acid ChEBI	-	assimilation
[Ref.: #43648]	glycine ChEBI	-	assimilation
[Ref.: #68381]	glycogen ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #43648]	glycolate ChEBI	-	assimilation
[Ref.: #43648]	glyoxylate ChEBI	+/-	assimilation
[Ref.: #68381]	hippurate ChEBI	-	hydrolysis	* from API rID32STR
[Ref.: #43648]	inosine ChEBI	+	assimilation
[Ref.: #43648]	L-alanine ChEBI	-	assimilation
[Ref.: #43648]	L-arabinose ChEBI	-	assimilation
[Ref.: #68369]	L-arabinose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68381]	L-arabinose ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #43648]	L-arginine ChEBI	+/-	assimilation
[Ref.: #43648]	L-asparagine ChEBI	+/-	assimilation
[Ref.: #43648]	L-cystine ChEBI	+/-	assimilation
[Ref.: #43648]	L-fucose ChEBI	-	assimilation
[Ref.: #43648]	L-glutamate ChEBI	-	assimilation
[Ref.: #43648]	L-proline ChEBI	-	assimilation
[Ref.: #43648]	L-serine ChEBI	-	assimilation
[Ref.: #43648]	lactate ChEBI	-	assimilation
[Ref.: #68381]	lactose ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68369]	malate ChEBI	-	assimilation	* from API 20NE
[Ref.: #43648]	maltose ChEBI	-	assimilation
[Ref.: #68369]	maltose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68381]	maltose ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68381]	melezitose ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68381]	melibiose ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #43648]	mesaconate ChEBI	-	assimilation
[Ref.: #68381]	methyl beta-D-glucopyranoside ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #43648]	myo-inositol ChEBI	+/-	assimilation
[Ref.: #43648]	nitrate ChEBI	-	reduction
[Ref.: #68369]	nitrate ChEBI	-	reduction	* from API 20NE
[Ref.: #43648]	nitrite ChEBI	-	reduction
[Ref.: #43648]	palatinose ChEBI	-	assimilation
[Ref.: #43648]	propionate ChEBI	-	assimilation
[Ref.: #68381]	pullulan ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68381]	raffinose ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #43648]	ribitol ChEBI	-	assimilation
[Ref.: #68381]	sorbitol ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #43648]	sucrose ChEBI	-	assimilation
[Ref.: #68381]	sucrose ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68381]	trehalose ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68369]	tryptophan ChEBI	-	energy source	* from API 20NE
[Ref.: #68369]	urea ChEBI	+	hydrolysis	* from API 20NE, API rID32STR
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Production
[Ref.: #43648]	acetoin ChEBI	yes
[Ref.: #43648]	hydrogen sulfide ChEBI	no
[Ref.: #68381]	acetoin ChEBI	no	* from API rID32STR
[Ref.: #68369]	indole ChEBI	no	* from API 20NE

	Metabolite	Voges-Proskauer-test	Indole test
[Ref.: #43648]	acetoin ChEBI	+
[Ref.: #68381]	acetoin ChEBI	-		* from API rID32STR
[Ref.: #68369]	indole ChEBI		-	* from API 20NE

	Enzyme	Enzyme activity	EC number
[Ref.: #68381]	Alanyl-Phenylalanyl-Proline arylamidase	+		* from API rID32STR
[Ref.: #68381]	alkaline phosphatase	+	3.1.3.1	* from API rID32STR
[Ref.: #68381]	alpha-galactosidase	-	3.2.1.22	* from API rID32STR
[Ref.: #68381]	arginine dihydrolase	+	3.5.3.6	* from API rID32STR
[Ref.: #68369]	arginine dihydrolase	-	3.5.3.6	* from API 20NE
[Ref.: #68381]	beta-galactosidase	-	3.2.1.23	* from API rID32STR
[Ref.: #68381]	beta-glucosidase	-	3.2.1.21	* from API rID32STR, API 20NE
[Ref.: #68381]	beta-glucuronidase	-	3.2.1.31	* from API rID32STR
[Ref.: #68381]	beta-mannosidase	-	3.2.1.25	* from API rID32STR
[Ref.: #43648]	catalase	+	1.11.1.6
[Ref.: #43648]	cytochrome oxidase	-	1.9.3.1
[Ref.: #68369]	cytochrome oxidase	-	1.9.3.1	* from API 20NE
[Ref.: #68369]	gelatinase	-		* from API 20NE
[Ref.: #68381]	glycyl tryptophan arylamidase	+		* from API rID32STR
[Ref.: #68381]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API rID32STR
[Ref.: #43648]	nitrate reductase	-	1.7.99.4
[Ref.: #68381]	pyrrolidonyl arylamidase	-	3.4.19.3	* from API rID32STR
[Ref.: #43648]	urease	+	3.5.1.5
[Ref.: #68381]	urease	+	3.5.1.5	* from API rID32STR, API 20NE
	Only first 10 entries are displayed. Click here to see all.

API® 20NE:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitratesNO3	Indole productionTRP	Fermentation of D-glucoseGLU_ Ferm	Arginine dihydrolaseADH (Arg)	Urease/urea hydrolysisURE	Esculin hydrolysis/beta-GlucosidaseESC	Gelatinase/gelatin hydrolysisGEL	beta-GalactosidasePNPG	Assimilation of D-glucoseGLU_ Assim	Assimilation of L-arabinoseARA	Assimilation of D-mannoseMNE	Assimilation of D-mannitolMAN	Assimilation of N-acetyl-glucosamineNAG	Assimilation of maltoseMAL	Assimilation of gluconateGNT	Assimilation of capric acidCAP	Assimilation adipic acidADI	Assimilation of malic acidMLT	Assimilation of citric acidCIT	Assimilation of phenyl-acetic acidPAC	Cytochrome oxidaseOX
[Ref.: #24317]	24616	-	-	-	-	+	-	-	-	+/-	-	+/-	-	-	-	-	-	-	-	-	-	-
[Ref.: #24317]	24615	-	-	-	-	+	-	-	-	+/-	-	+	-	+/-	-	-	-	-	-	-	-	-

API® rID32STR:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Arginine dihydrolaseADH (Arg)	beta-Glucosidasebeta GLU	beta-Galactosidasebeta GAR	beta-Glucuronidasebeta GUR	alpha-Galactosidasealpha GAL	Alkaline PhosphatasePAL	Acid from D-riboseRIB	Acid from D-mannitolMAN	Acid from sorbitolSOR	Acid from lactoseLAC	Acid from trehaloseTRE	Acid from raffinoseRAF	Acid from sucroseSAC	Acid from L-arabinoseLARA	Acid from D-arabitolDARL	Acid from alpha-cyclodextrinCDEX	Acetoin production (Voges Proskauer test)VP	Alanyl-Phenylalanyl-Proline arylamidaseAPPA	beta-Galactosidasebeta GAL	Pyrrolidonyl arylamidasePyrA	N-Acetyl-glucosaminidasebeta NAG	Glycyl-tryptophan arylamidaseGTA	Hydrolysis of hippuric acidHIP	Acid from glycogenGLYG	Acid from pullulanPUL	Acid from maltoseMAL	Acid from melibioseMEL	Acid from melezitoseMLZ	Acidification of methyl beta-D-glucopyranosideMbeta DG	Acid from D-tagatoseTAG	beta-Mannosidasebeta MAN	Urease/urea hydrolysisURE
[Ref.: #24317]	10752	+	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	+	-	-	-	-	-	-	-	-	-	+
[Ref.: #24317]	10751	+	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	+	-	-	-	-	-	-	-	-	-	+

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #24317]	Sample type/isolated from	mandibular lymph nodes of a red foxes (Vulpes vulpes)
[Ref.: #24317]	Host species	Vulpes vulpes
[Ref.: #24317]	Geographic location (country and/or sea, region)	Lower Austria
[Ref.: #24317]	Country	Austria
[Ref.: #24317]	Country ISO 3 Code	AUT
[Ref.: #24317]	Continent	Europe

[Ref.: #43648]	Sample type/isolated from	mandibular lymph nodes of a red fox (Vulpes vulpes)
[Ref.: #43648]	Geographic location (country and/or sea, region)	Lower Austria
[Ref.: #43648]	Country	Austria
[Ref.: #43648]	Country ISO 3 Code	AUT
[Ref.: #43648]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Host Body-Site	#Oral cavity and airways	-
#Host	#Mammals	#Canidae (Dog)
#Host Body-Site	#Organ	#Lymph node

What are isolation sources categories?

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Brucella vulpis F60	GCA_900000005	complete	GenBank	981386 tax ID	*

[Ref.: #43648]	GC-content	57.2 mol%
[Ref.: #24317]	GC-content	57.2 mol%	sequence analysis

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Brucella vulpis F60 NCBI: GCA_900000005.1
[Ref.: #69480]	motile	no	67.01	no
[Ref.: #69480]	flagellated	no	87.715	no
[Ref.: #69480]	gram-positive	no	98.537	yes
[Ref.: #69480]	anaerobic	no	98.408	no
[Ref.: #69480]	aerobic	yes	90.544	yes
[Ref.: #69480]	halophile	no	87.136	no
[Ref.: #69480]	spore-forming	no	96.402	yes
[Ref.: #69480]	glucose-ferment	no	89.283	yes
[Ref.: #69480]	thermophile	no	93.354	yes
[Ref.: #69480]	glucose-util	yes	83.801	yes

Availability in culture collections External links

[Ref.: #24317]

Culture collection no.

DSM 101715, BCCN 09-2

[Ref.: #90854]

SI-ID 403993

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Brucella vulpis sp. nov., isolated from mandibular lymph nodes of red foxes (Vulpes vulpes).	Scholz HC, Revilla-Fernandez S, Dahouk SA, Hammerl JA, Zygmunt MS, Cloeckaert A, Koylass M, Whatmore AM, Blom J, Vergnaud G, Witte A, Aistleitner K, Hofer E	Int J Syst Evol Microbiol	10.1099/ijsem.0.000998	2016	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#24317

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 101715

#43648

Holger C. Scholz, Sandra Revilla-Fernandez, Sascha Al Dahouk, Jens A. Hammerl, Michel S. Zygmunt, Axel Cloeckaert, Mark Koylass, Adrian M. Whatmore, Jochen Blom, Gilles Vergnaud, Angela Witte, Karin Aistleitner, Erwin Hofer: Brucella vulpis sp. nov., isolated from mandibular lymph nodes of red foxes (Vulpes vulpes). IJSEM 66: 2090 - 2098 2016 ( DOI 10.1099/ijsem.0.000998 , PubMed 26928956 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68369

Automatically annotated from API 20NE .

#68381

Automatically annotated from API rID32STR .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#90854

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID403993.1 )

* These data were automatically processed and therefore are not curated

Change proposal

You found an error in BacDive? Please tell us about it!

Note that changes will be reviewed and judged. If your changes are legitimate, changes will occur within the next BacDive update. Only proposed changes supported by the according reference will be reviewed. The BacDive team reserves the right to reject proposed changes.

Search for species Brucella vulpis in external resources:
SILVA
BRENDA
PANGAEA
StrainInfo
GBIF