Name: Sulfuriferula thiophila DSM 101871
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 132527

Type strain:

Species: Sulfuriferula thiophila

Culture col. no.: DSM 101871, NBRC 111150, MSt mst6, MSt 6

Strain history: <- T. Watanabe, Hokkaido University, Sapporo, Japan; mst6 <- T. Watanabe {2014}

NCBI tax ID(s): 1781211 (species)

SI-ID 401433

DSM 101871

Section

Sulfuriferula thiophila DSM 101871 is an aerobe, Gram-negative, motile bacterium that was isolated from hot spring water from a storage tank for a public bath.

Gram-negative
motile
rod-shaped
aerobe
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Betaproteobacteria*
	Order	*Nitrosomonadales*
	Family	*Sulfuricellaceae*
	Genus	*Sulfuriferula*
	Species	**Sulfuriferula thiophila**
	Full Scientific Name (LPSN)	Sulfuriferula thiophila Watanabe et al. 2016

Information on morphological and physiological properties Morphology

[Ref.: #43987]	Gram stain	negative

[Ref.: #43987]	Cell length	1.2-4.0 µm

[Ref.: #43987]	Cell width	0.5-0.7 µm

[Ref.: #43987]	Cell shape	rod-shaped

[Ref.: #43987]	Motility	yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #43987]

Culture medium

bicarbonate-buffered low-salt defined medium

[Ref.: #43987]

Culture medium growth

[Ref.: #43987]

Culture medium composition

expand / minimize

[Ref.: #24316]

Culture medium

SULFURIFERULA THIOPHILA MEDIUM (DSMZ Medium 1585a)

[Ref.: #24316]

Culture medium growth

[Ref.: #24316]

Culture medium link

Medium recipe at MediaDive

[Ref.: #24316]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #24316]	growth	30 °C
[Ref.: #43987]	growth	5-34 °C
[Ref.: #43987]	optimum	32 °C

[Ref.: #24316]	Temperature range	mesophilic
[Ref.: #43987]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #43987]			optimum	5.9-6.2
[Ref.: #43987]			growth	4.6-8.1

Information on physiology and metabolism Physiology and metabolism

[Ref.: #43987]

Oxygen tolerance

aerobe

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #43987]		NaCl		growth	0-0.110	M
[Ref.: #43987]		NaCl		optimum	0	M

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #43987]	elemental sulfur ChEBI	+	electron donor
[Ref.: #43987]	hydrogen sulfide ChEBI	-	electron donor
[Ref.: #43987]	nitrate ChEBI	-	reduction
[Ref.: #43987]	tetrathionate ChEBI	+	electron donor
[Ref.: #43987]	thiosulfate ChEBI	+	electron donor

	Enzymes	Enzyme	Enzyme activity	EC number
[Ref.: #43987]		catalase	+	1.11.1.6
[Ref.: #43987]		cytochrome oxidase	+	1.9.3.1

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #24316]	Sample type/isolated from	hot spring water from a storage tank for a public bath
[Ref.: #24316]	Geographic location (country and/or sea, region)	Yamanashi Prefecture, Hokuto City, Masutomi hot spring
[Ref.: #24316]	Country	Japan
[Ref.: #24316]	Country ISO 3 Code	JPN
[Ref.: #24316]	Continent	Asia
[Ref.: #24316]	Geographic location	35.8647°/138.5300°

[Ref.: #43987]	Sample type/isolated from	spring water sample obtained from a storage tank at Masutomi hot spring located in Hokuto City, Yamanashi Prefecture, Japan
[Ref.: #43987]	Sampling date	2014-06
[Ref.: #43987]	Geographic location (country and/or sea, region)	Masutomi hot spring located in Hokuto City, Yamanashi Prefecture
[Ref.: #43987]	Country	Japan
[Ref.: #43987]	Country ISO 3 Code	JPN
[Ref.: #43987]	Continent	Asia
[Ref.: #43987]	Geographic location	35.8647°/138.5300°
[Ref.: #43987]	Enrichment culture	bicarbonate-buffered low-salt defined medium
[Ref.: #43987]	Enrichment culture composition	0.2 g/L MgCl2.6H2O, 0.1 g/L CaCl2.2H2O, 0.1 g/L NH4Cl, 0.1 g/L KH2PO4, 0.1 g/L KCl, 5 g/L Na2S2O3.5H2O, 1 ml trace element solution, 1 ml selenite-tungstate solution, 30 ml NaHCO3 solution, dissolved in 20 mM MOPS-NaOH, 1,5% agar, pH 7.1
* marker position based on {}

Isolation sources categories

#Engineered	#Built environment	#Water reservoir (Aquarium/pool)
#Condition	#Thermophilic (>45°C)	-
#Environmental	#Aquatic	#Spring
#Environmental	#Aquatic	#Thermal spring

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence LC115045 (>99% sequence identity) for Sulfuriferula thiophila subclade from Microbeatlas

Aquatic Samples	218
Soil Samples	68
Animal Samples	10
Plant Samples	1
Total Samples	297

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #24316]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #24316]	Sulfuriferula thiophila gene for 16S ribosomal RNA, partial sequence	LC115045	1460	ENA	1781211 tax ID

[Ref.: #24316]

GC-content

52.6 mol%

high performance liquid chromatography (HPLC)

Availability in culture collections External links

[Ref.: #24316]

Culture collection no.

DSM 101871, NBRC 111150, MSt mst6, MSt 6

[Ref.: #90853]

SI-ID 401433

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Sulfuriferula thiophila sp. nov., a chemolithoautotrophic sulfur-oxidizing bacterium, and correction of the name Sulfuriferula plumbophilusWatanabe, Kojima and Fukui 2015 to Sulfuriferula plumbiphila corrig.	Watanabe T, Kojima H, Fukui M	Int J Syst Evol Microbiol	10.1099/ijsem.0.000988	2016	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#24316

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 101871

#43987

Tomohiro Watanabe, Hisaya Kojima, Manabu Fukui: Sulfuriferula thiophila sp. nov., a chemolithoautotrophic sulfur-oxidizing bacterium, and correction of the name Sulfuriferula plumbophilus Watanabe, Kojima and Fukui 2015 to Sulfuriferula plumbiphila corrig.. IJSEM 66: 2041 - 2045 2016 ( DOI 10.1099/ijsem.0.000988 , PubMed 26908287 )

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#90853

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID401433.1 )

* These data were automatically processed and therefore are not curated

Change proposal

You found an error in BacDive? Please tell us about it!

Note that changes will be reviewed and judged. If your changes are legitimate, changes will occur within the next BacDive update. Only proposed changes supported by the according reference will be reviewed. The BacDive team reserves the right to reject proposed changes.

Search for species Sulfuriferula thiophila in external resources:
SILVA
BRENDA
PANGAEA
StrainInfo
GBIF