Thermithiobacillus plumbiphilus (DSM 101799, NBRC 111508, wk12)

Name: Thermithiobacillus plumbiphilus (DSM 101799, NBRC 111508, wk12)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 132526

Type strain

Strain Designation wk12

Culture col. no. DSM 101799 NBRC 111508 wk12

NCBI tax ID(s) 1729899

Special Collections DSMZ Literature strains

Links

Thermithiobacillus plumbiphilus wk12 is a Gram-negative, motile, rod-shaped prokaryote that was isolated from industrially synthesized lead sulfide.

Gram-negative motile rod-shaped 16S sequence

Name and taxonomic classification

SI-ID 408113

NBRC 111508,DSM 101799

@ref 20215

Last LPSN update 2025-12-16 10:01:32

Domain Pseudomonadati

Phylum Pseudomonadota

Class Acidithiobacillia

Order Acidithiobacillales

Family Thermithiobacillaceae

Genus Thermithiobacillus

Species Thermithiobacillus plumbiphilus

Full scientific name Thermithiobacillus plumbiphilus Watanabe et al. 2016

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape	Motility
43988	negative	0.7-2.2 µm	0.5-1.0 µm	rod-shaped

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
24315	SULFURIFERULA THIOPHILA MEDIUM (DSMZ Medium 1585a)		Medium recipe at MediaDive	Name: SULFURIFERULA THIOPHILA MEDIUM (DSMZ Medium 1585a) Composition: Na2S2O3 x 5 H2O 5.0 g/l MOPS 4.2 g/l NaHCO3 2.5 g/l MgCl2 x 6 H2O 0.2 g/l KCl 0.1 g/l KH2PO4 0.1 g/l (NH4)2SO4 0.1 g/l CaCl2 x 2 H2O 0.1 g/l HCl 0.0025 g/l FeCl2 x 4 H2O 0.0015 g/l NaOH 0.0005 g/l CoCl2 x 6 H2O 0.00019 g/l MnCl2 x 4 H2O 0.0001 g/l ZnCl2 7e-05 g/l Na2MoO4 x 2 H2O 3.6e-05 g/l NiCl2 x 6 H2O 2.4e-05 g/l H3BO3 6e-06 g/l Na2WO4 x 2 H2O 4e-06 g/l Na2SeO3 x 5 H2O 3e-06 g/l CuCl2 x 2 H2O 2e-06 g/l Distilled water
43988	bicarbonate-buffered low-salt defined medium			0.2 g/L MgCl2.6H2O, 0.1 g/L CaCl2.2H2O, 0.1 g/L KH4Cl, 0.1 g/L KH2PO4, 0.1 g/L KCl, 1 ml trace element solution, 1 ml selenite-tungstate solution, 1 ml vitamin solution DSM 141, 10 ml 1M Na2S2O3 solution, 30 ml 1M NaHCO3 solution

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
24315	positive	growth	30	mesophilic
43988	positive	growth	5-37
43988	positive	optimum	28-32	mesophilic

Culture pH

@ref	Ability	Type	PH	PH range
43988	positive	optimum	6.4-7.1
43988	positive	growth	5.8-8.7	alkaliphile

Physiology and metabolism

Spore formation

43988

Spore formationno

Halophily

@ref	Salt	Growth	Tested relation	Concentration
43988	NaCl	positive	optimum	0 M
43988	NaCl	positive	growth	0.4-2.5

Enzymes

@ref	Value	Activity	Ec
43988	catalase	-	1.11.1.6
43988	cytochrome oxidase	-	1.9.3.1

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Engineered	#Industrial	#Industrial production

Isolation

@ref	Sample type	Country	Country ISO 3 Code	Continent	Enrichment culture	Enrichment culture composition	Enrichment culture temperature
24315	industrially synthesized lead (II) sulfide	Japan	JPN	Asia
43988	industrially synthesized lead (II) sulfide				modified ATCC 290 S6 medium	3 g/L Na2HPO4.12H2O, 1.8 g/L KH2PO4, 0.1 g/L MgSO4.7H2O, 0.1 g/L (NH4)2SO4, 0.04 g/L CaCl2 . 2H2O, 0.03 g FeCl3.6H2O, 0.03 g/L MnSO4.5H2O, 10 g/L Na2S2O3, with decreased thiosulfate concentration to 0.4 mM	22

Taxonmaps

69479

Global distribution of 16S sequence LC088006 (>99% sequence identity) for Thermithiobacillus plumbiphilus from Microbeatlas

Aquatic Samples	116
Soil Samples	276
Animal Samples	15
Plant Samples	14
Total Samples	421

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
24315	1	Risk group (German classification)

Sequence information

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
24315	Thermithiobacillus plumbiphilus gene for 16S ribosomal RNA, partial sequence	LC088006	1419		1729899

GC content

@ref	GC-content (mol%)	Method
24315	58.5	high performance liquid chromatography (HPLC)

Genome-based predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Thermithiobacillus plumbiphilus sp. nov., a sulfur-oxidizing bacterium isolated from lead sulfide.	Watanabe T, Miura A, Shinohara A, Kojima H, Fukui M	Int J Syst Evol Microbiol	10.1099/ijsem.0.000972	2016

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#24315	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 101799
#43988	Tomohiro Watanabe, Aya Miura, Arisa Shinohara, Hisaya Kojima, Manabu Fukui: Thermithiobacillus plumbiphilus sp. nov., a sulfur-oxidizing bacterium isolated from lead sulfide. IJSEM 66: 1986 - 1989 2016 ( DOI 10.1099/ijsem.0.000972 , PubMed 26873326 )
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Thermithiobacillus plumbiphilus (DSM 101799, NBRC 111508, wk12)

Name and taxonomic classification

Morphology

Cell morphology

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Spore formation

Halophily

Enzymes

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

16S sequences

GC content

Genome-based predictions

Literature

Literature

References

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