Name: Phytoactinopolyspora alkaliphila DSM 101529
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 132519

Type strain:

Species: Phytoactinopolyspora alkaliphila

Culture col. no.: DSM 101529, CGMCC 4.7225, KCTC 39701, EGI 80629, JCM 32495

Strain history: CGMCC 4.7225 <-- Y.-G. Zhang; Xinjiang Inst. of Ecol. & Geogr., CAS, China; EGI 80629.

NCBI tax ID(s): 1783498 (species)

SI-ID 406689

DSM 101529

Section

Phytoactinopolyspora alkaliphila DSM 101529 is an aerobe, spore-forming, Gram-positive bacterium that was isolated from saline-alkaline desert soil.

Gram-positive
rod-shaped
spore-forming
aerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Jiangellales*
	Family	*Jiangellaceae*
	Genus	*Phytoactinopolyspora*
	Species	**Phytoactinopolyspora alkaliphila**
	Full Scientific Name (LPSN)	Phytoactinopolyspora alkaliphila Zhang et al. 2016

Information on morphological and physiological properties Morphology

[Ref.: #43661]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #43661]	Cell shape	rod-shaped

[Ref.: #69480]	Motility	no Confidence in %91.04

Multimedia content

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Caption:

Medium 535 37°C

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Intellectual property rights:

Information on culture and growth conditions Culture and growth conditions

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Culture medium

BHI MEDIUM (DSMZ Medium 215)

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Culture medium growth

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Culture medium link

Medium recipe provided by DSMZ

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Culture medium

TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

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Culture medium composition

expand / minimize

		Temperature
[Ref.: #24308]	growth	37 °C
[Ref.: #43661]	growth	15-45 °C
[Ref.: #43661]	optimum	30 °C
[Ref.: #67770]	growth	37 °C

[Ref.: #24308]	Temperature range	mesophilic
[Ref.: #43661]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #43661]			optimum	9-10
[Ref.: #43661]			growth	6-11

Information on physiology and metabolism Physiology and metabolism

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Oxygen tolerance

aerobe

[Ref.: #43661]	Spore description	short chains of spores; spore chains do not aggregate at maturity
[Ref.: #43661]	Type of spore	spore
[Ref.: #43661]	Ability of spore formation	yes
[Ref.: #69481]	Ability of spore formation	yes Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %92.322

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #43661]		NaCl		growth	0-13	%(w/v)
[Ref.: #43661]		NaCl		optimum	3-5	%(w/v)

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Observation

quinones: MK-9(H₄)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #43661]	cellulose ChEBI	-	hydrolysis
[Ref.: #43661]	D-arabinose ChEBI	+	carbon source
[Ref.: #43661]	D-fructose ChEBI	+	carbon source
[Ref.: #43661]	D-galactose ChEBI	+	carbon source
[Ref.: #43661]	D-glucose ChEBI	+	carbon source
[Ref.: #43661]	D-mannose ChEBI	+	carbon source
[Ref.: #43661]	D-ribose ChEBI	-	carbon source
[Ref.: #43661]	D-sorbitol ChEBI	+	carbon source
[Ref.: #43661]	D-xylose ChEBI	+	carbon source
[Ref.: #43661]	dl-alanine ChEBI	+	nitrogen source
[Ref.: #43661]	gelatin ChEBI	+	hydrolysis
[Ref.: #43661]	glycerol ChEBI	+	carbon source
[Ref.: #43661]	L-arginine ChEBI	-	nitrogen source
[Ref.: #43661]	L-asparagine ChEBI	+	nitrogen source
[Ref.: #43661]	L-aspartate ChEBI	+	nitrogen source
[Ref.: #43661]	L-cystine ChEBI	+	nitrogen source
[Ref.: #43661]	L-glutamate ChEBI	+	nitrogen source
[Ref.: #43661]	L-histidine ChEBI	+	nitrogen source
[Ref.: #43661]	L-isoleucine ChEBI	+	nitrogen source
[Ref.: #43661]	L-lysine ChEBI	+	nitrogen source
[Ref.: #43661]	L-methionine ChEBI	+	nitrogen source
[Ref.: #43661]	L-phenylalanine ChEBI	+	nitrogen source
[Ref.: #43661]	L-rhamnose ChEBI	+	carbon source
[Ref.: #43661]	L-serine ChEBI	+	nitrogen source
[Ref.: #43661]	L-sorbose ChEBI	+	carbon source
[Ref.: #43661]	L-threonine ChEBI	+	nitrogen source
[Ref.: #43661]	L-tryptophan ChEBI	+	nitrogen source
[Ref.: #43661]	L-tyrosine ChEBI	-	nitrogen source
[Ref.: #43661]	L-valine ChEBI	+	nitrogen source
[Ref.: #43661]	lactose ChEBI	+	carbon source
[Ref.: #43661]	malate ChEBI	+	carbon source
[Ref.: #43661]	maltose ChEBI	+	carbon source
[Ref.: #43661]	myo-inositol ChEBI	+	carbon source
[Ref.: #43661]	nitrate ChEBI	-	reduction
[Ref.: #43661]	raffinose ChEBI	+	carbon source
[Ref.: #43661]	sodium citrate ChEBI	+	carbon source
[Ref.: #43661]	sodium pyruvate ChEBI	+	carbon source
[Ref.: #43661]	starch ChEBI	-	hydrolysis
[Ref.: #43661]	sucrose ChEBI	+	carbon source
[Ref.: #43661]	trehalose ChEBI	+	carbon source
[Ref.: #43661]	tween 20 ChEBI	+	hydrolysis
[Ref.: #43661]	tween 40 ChEBI	+	hydrolysis
[Ref.: #43661]	tween 60 ChEBI	+	hydrolysis
[Ref.: #43661]	tween 80 ChEBI	+	hydrolysis
[Ref.: #43661]	xylitol ChEBI	+	carbon source
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Antibiotic sensitivity	Concentration		Antibiotic resistance	Concentration
[Ref.: #43661]	amphotericin b ChEBI	yes	30	µg (disc)
[Ref.: #43661]	ampicillin ChEBI	yes	10	µg (disc)
[Ref.: #43661]	azithromycin ChEBI				yes	15	µg
[Ref.: #43661]	cefalotin ChEBI	yes	30	µg
[Ref.: #43661]	chloramphenicol ChEBI	yes	30	µg (disc)
[Ref.: #43661]	clarithromycin ChEBI				yes	15	µg
[Ref.: #43661]	erythromycin ChEBI				yes	15	µg
[Ref.: #43661]	gentamicin ChEBI	yes	10	µg (disc)
[Ref.: #43661]	kanamycin ChEBI				yes	30	µg
[Ref.: #43661]	lincomycin ChEBI	yes	2	µg (disc)
[Ref.: #43661]	neomycin ChEBI				yes	30	µg
[Ref.: #43661]	novobiocin ChEBI	yes	30	µg (disc)
[Ref.: #43661]	penicillin g ChEBI	yes	10	µg (disc)
[Ref.: #43661]	polymyxin b ChEBI	yes	300	µg (disc)
[Ref.: #43661]	roxithromycin ChEBI				yes	15	µg
[Ref.: #43661]	spectinomycin ChEBI	yes	100	µg
[Ref.: #43661]	streptomycin ChEBI	yes	10	µg
[Ref.: #43661]	tetracycline ChEBI				yes	30	µg
[Ref.: #43661]	vancomycin ChEBI	yes	30	µg
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #43661]		hydrogen sulfide ChEBI	no

	Enzymes	Enzyme	Enzyme activity	EC number
[Ref.: #43661]		catalase	+	1.11.1.6
[Ref.: #43661]		cytochrome oxidase	-	1.9.3.1

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #24308]	Sample type/isolated from	saline-alkaline desert soil
[Ref.: #24308]	Geographic location (country and/or sea, region)	Gurbantünggüt Desert, Xinjiang, Karamay
[Ref.: #24308]	Country	China
[Ref.: #24308]	Country ISO 3 Code	CHN
[Ref.: #24308]	Continent	Asia

[Ref.: #43661]	Sample type/isolated from	A soi sample collected in Karamay
[Ref.: #43661]	Geographic location (country and/or sea, region)	Karamay, Xinjiang
[Ref.: #43661]	Country	China
[Ref.: #43661]	Country ISO 3 Code	CHN
[Ref.: #43661]	Continent	Asia

[Ref.: #67770]	Sample type/isolated from	Soil in Karamay
[Ref.: #67770]	Geographic location (country and/or sea, region)	Xinjiang, north-west China
[Ref.: #67770]	Country	China
[Ref.: #67770]	Country ISO 3 Code	CHN
[Ref.: #67770]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Environmental	#Terrestrial	#Desert
#Condition	#Alkaline	-
#Condition	#Saline	-
#Environmental	#Terrestrial	#Soil

What are isolation sources categories?

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Global distribution of 16S sequence KU042078 (>99% sequence identity) for Phytoactinopolyspora alkaliphila subclade from Microbeatlas

Aquatic Samples	11
Soil Samples	123
Animal Samples	27
Plant Samples	3
Total Samples	164

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

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Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #24308]	Phytoactinopolyspora alkaliphila strain EGI 80629 16S ribosomal RNA gene, partial sequence	KU042078	1518	ENA	1783498 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Phytoactinopolyspora alkaliphila CGMCC 4.7225	GCA_010550695	scaffold	GenBank	1783498 tax ID
[Ref.: #66792]	Phytoactinopolyspora alkaliphila strain CGMCC 4.7225	1783498.3	wgs	PATRIC	1783498 tax ID	*

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GC-content

67.3 mol%

high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Phytoactinopolyspora alkaliphila CGMCC 4.7225 NCBI: GCA_010550695.1
[Ref.: #69481]	spore-forming	yes	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Phytoactinopolyspora alkaliphila strain CGMCC 4.7225 PATRIC: 1783498.3
[Ref.: #69480]	motile	no	86.047	no
[Ref.: #69480]	gram-positive	yes	91.023	yes
[Ref.: #69480]	anaerobic	no	98.498	no
[Ref.: #69480]	aerobic	yes	92.857	yes
[Ref.: #69480]	halophile	no	54.702	no
[Ref.: #69480]	spore-forming	yes	60.372	yes
[Ref.: #69480]	thermophile	no	94.231	no
[Ref.: #69480]	glucose-util	yes	88.014	no
[Ref.: #69480]	flagellated	no	98.126	no
[Ref.: #69480]	glucose-ferment	no	90.183	no

Availability in culture collections External links

[Ref.: #24308]

Culture collection no.

DSM 101529, CGMCC 4.7225, KCTC 39701, EGI 80629, JCM 32495

[Ref.: #90845]

SI-ID 406689

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Phytoactinopolyspora halophila sp. nov., a halophilic actinomycete isolated from a saline soil.	Ding ZG, Ji Y, Yin M, Zhao YR, Feng YZ, Chunyu WX, Tang SK	Int J Syst Evol Microbiol	10.1099/ijsem.0.003153	2018	*
Phylogeny	Phytoactinopolyspora halotolerans sp. nov., a halotolerant actinobacterium isolated from a saline soil in Xinjiang, northwest of China.	Ji Y, Chunyu WX, Li EY, Ding ZG, Yin M, Zhao YR, Wang Y, Tang SK	Antonie Van Leeuwenhoek	10.1007/s10482-017-0923-6	2017	*
Phylogeny	Phytoactinopolyspora alkaliphila sp. nov., an alkaliphilic actinomycete isolated from a saline-alkaline soil.	Zhang YG, Lu XH, Ding YB, Zhou XK, Li L, Guo JW, Wang HF, Duan YQ, Li WJ	Int J Syst Evol Microbiol	10.1099/ijsem.0.000992	2016	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#24308

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 101529

#43661

Yong-Guang Zhang, Xin-Hua Lu, Yan-Bo Ding, Xing-Kui Zhou, Li Li, Jian-Wei Guo, Hong-Fei Wang, Yan-Qing Duan, Wen-Jun Li: Phytoactinopolyspora alkaliphila sp. nov., an alkaliphilic actinomycete isolated from a saline-alkaline soil. IJSEM 66: 2058 - 2063 2016 ( DOI 10.1099/ijsem.0.000992 , PubMed 26920762 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#90845

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID406689.1 )

* These data were automatically processed and therefore are not curated

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Phytoactinopolyspora alkaliphila CGMCC 4.7225

Phytoactinopolyspora alkaliphila strain CGMCC 4.7225

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