Nocardioides zeicaulis (DSM 101604, CCM 8654, CIP 110980)

Name: Nocardioides zeicaulis (DSM 101604, CCM 8654, CIP 110980)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 132482

Type strain

Strain Designation JM-601

Culture col. no. DSM 101604 CCM 8654 CIP 110980 JM-601 JCM 32381

NCBI tax ID(s) 1776857

Special Collections DSMZ JCM CIP Literature strains

History <- P. Kämpfer, Justus-Liebig-Universität, Giessen, Germany; JM-601 <- J. McInroy CCM 8654 <-- S. P. Glaeser; Justus-Liebig-Univ. Giessen, Germany; JM-601 <-- J. A. McInroy; Auburn Univ., USA. CIP <- 2015, P. Kämpfer, Giessen Univ., Giessen, Germany: strain JM-601

Links

Nocardioides zeicaulis JM-601 is an obligate aerobe, mesophilic, Gram-positive prokaryote that forms circular colonies and was isolated from stem tissue of healthy 10-week-old, field-grown maize , isolated as an endophyte.

Gram-positive rod-shaped colony-forming obligate aerobe mesophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 403774

JCM 32381,DSM 101604,CIP 110980

@ref 20215

Last LPSN update 2026-02-09 11:31:43

Domain Bacteria

Phylum Actinomycetota

Class Actinomycetes

Order Propionibacteriales

Family Nocardioidaceae

Genus Nocardioides

Species Nocardioides zeicaulis

Full scientific name Nocardioides zeicaulis Kämpfer et al. 2016

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape	Motility
43685	positive	1.5-2.5 µm	0.9-1.5 µm	rod-shaped
124030	positive			rod-shaped

Colony morphology

@ref	Colony size	Colony color	Colony shape	Incubation period	Medium used
43685	0.5 mm	Yellow	circular	2 days	TSA agar

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
24271	TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)	Medium recipe at MediaDive	Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water
42168	MEDIUM 72- for trypto casein soja agar		Distilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
43685	Trypticase Soy Agar (TSA)
24271	R2A MEDIUM (DSMZ Medium 830)	Medium recipe at MediaDive	Name: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water
124030	CIP Medium 72	Medium recipe at CIP
43685	Trypticase Soy Agar (TSA)

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
24271	positive	growth	28	mesophilic
42168	positive	growth	30	mesophilic
43685	positive	growth	10-37
43685	positive	optimum	28	mesophilic
67770	positive	growth	28	mesophilic

Culture pH

@ref	Ability	Type	PH
43685	positive	optimum	7
43685	positive	growth	5.5-8

Physiology and metabolism

Oxygen tolerance

43685

Oxygen toleranceobligate aerobe

Spore formation

@ref	Type of spore	Spore formation
43685	endospore

Halophily

@ref	Salt	Growth	Tested relation	Concentration
43685	NaCl	positive	growth	1-3 %

Observation

67770

Observationquinones: MK-8(H₄)

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
43685	17128 ChEBI	adipate	-	assimilation
43685	27613 ChEBI	amygdalin	-	fermentation
43685	18305 ChEBI	arbutin	-	assimilation
43685	17057 ChEBI	cellobiose	-	builds acid from
43685	17057 ChEBI	cellobiose	+	assimilation
43685	18333 ChEBI	D-arabitol	-	builds acid from
43685	15824 ChEBI	D-fructose	+	assimilation
43685	12936 ChEBI	D-galactose	+	assimilation
43685	18391 ChEBI	D-gluconate	+	assimilation
43685	17634 ChEBI	D-glucose	-	builds acid from
43685	17634 ChEBI	D-glucose	+	assimilation
43685	16899 ChEBI	D-mannitol	-	builds acid from
43685	16024 ChEBI	D-mannose	+	assimilation
43685	17924 ChEBI	D-sorbitol	-	assimilation
43685	17924 ChEBI	D-sorbitol	-	builds acid from
43685	65327 ChEBI	D-xylose	-	builds acid from
43685	65327 ChEBI	D-xylose	+	assimilation
43685	27689 ChEBI	decanoate	-	assimilation
43685	17113 ChEBI	erythritol	-	builds acid from
43685	16813 ChEBI	galactitol	-	builds acid from
43685	17234 ChEBI	glucose	-	fermentation
43685	17234 ChEBI	glucose	+	assimilation
43685	28087 ChEBI	glycogen	-	fermentation
43685	30849 ChEBI	L-arabinose	-	builds acid from
43685	30849 ChEBI	L-arabinose	-	fermentation
43685	30849 ChEBI	L-arabinose	+	assimilation
43685	62345 ChEBI	L-rhamnose	-	builds acid from
43685	62345 ChEBI	L-rhamnose	+	assimilation
43685	17716 ChEBI	lactose	-	builds acid from
43685	17716 ChEBI	lactose	-	fermentation
43685	25115 ChEBI	malate	-	assimilation
43685	17306 ChEBI	maltose	-	builds acid from
43685	17306 ChEBI	maltose	-	fermentation
43685	17306 ChEBI	maltose	+	assimilation
43685	29864 ChEBI	mannitol	-	fermentation
43685	28053 ChEBI	melibiose	-	builds acid from
43685	28053 ChEBI	melibiose	-	fermentation
43685	28053 ChEBI	melibiose	+	assimilation
43685	17268 ChEBI	myo-inositol	-	assimilation
43685	17268 ChEBI	myo-inositol	-	builds acid from
43685	17268 ChEBI	myo-inositol	-	fermentation
43685	28037 ChEBI	N-acetylgalactosamine	-	assimilation
43685	506227 ChEBI	N-acetylglucosamine	-	assimilation
43685	17632 ChEBI	nitrate	+	reduction
43685	18401 ChEBI	phenylacetate	-	assimilation
43685	16634 ChEBI	raffinose	-	builds acid from
43685	26546 ChEBI	rhamnose	-	fermentation
43685	15963 ChEBI	ribitol	-	assimilation
43685	15963 ChEBI	ribitol	-	builds acid from
43685	33942 ChEBI	ribose	-	assimilation
43685	33942 ChEBI	ribose	-	fermentation
43685	17814 ChEBI	salicin	-	assimilation
43685	17814 ChEBI	salicin	-	builds acid from
43685	53258 ChEBI	sodium citrate	-	assimilation
43685	30911 ChEBI	sorbitol	-	fermentation
43685	17992 ChEBI	sucrose	-	builds acid from
43685	17992 ChEBI	sucrose	-	fermentation
43685	17992 ChEBI	sucrose	+	assimilation
43685	27082 ChEBI	trehalose	-	builds acid from
43685	27082 ChEBI	trehalose	+	assimilation
43685	18222 ChEBI	xylose	-	fermentation

Metabolite production

@ref	Chebi-ID	Metabolite	Production
43685	16136 ChEBI	hydrogen sulfide
43685	35581 ChEBI	indole

Enzymes

@ref	Value	Activity	Ec
43685	alkaline phosphatase	+	3.1.3.1
43685	alpha-fucosidase	-	3.2.1.51
43685	alpha-galactosidase	+	3.2.1.22
43685	alpha-glucosidase	+	3.2.1.20
43685	alpha-mannosidase	-	3.2.1.24
43685	amylase	+
43685	arginine dihydrolase	-	3.5.3.6
43685	beta-galactosidase	+/-	3.2.1.23
43685	catalase	+	1.11.1.6
43685	cystine arylamidase	+	3.4.11.3
43685	cytochrome oxidase	-	1.9.3.1
43685	esterase Lipase (C 8)	+
43685	gelatinase	-
43685	leucine arylamidase	+	3.4.11.1
43685	lysine decarboxylase	-	4.1.1.18
43685	N-acetyl-beta-glucosaminidase	-	3.2.1.52
43685	ornithine decarboxylase	-	4.1.1.17
43685	protease	+
43685	pyrazinamidase	-	3.5.1.B15
43685	tryptophan deaminase	-	4.1.99.1
43685	urease	-	3.5.1.5
43685	valine arylamidase	+

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Engineered	#Agriculture	#Field
#Host	#Plants	#Herbaceous plants (Grass,Crops)
#Host Body-Site	#Plant	#Stem (Branch)

Isolation

@ref	Sample type	Host species	Geographic location	Country	Country ISO 3 Code	Continent	Isolation date
24271	stem tissue of healthy 10-week-old, field-grown maize (Zea mays, cultivar 'Sweet Belle'), isolated as an endophyte	Zea mays	Alabama, Tallassee Plant Breeding Unit facility, E.V. Smith Research Center	USA	USA	North America
43685	isolated as an endophyte of stem tissue of healthy maize (Zea mays)		Plant Breeding Unit facility, E. V. Smith Research Center, Tallassee, Alabama	USA	USA	North America	1990-07
67770	Stem tissue of healthy 10-week-old, field-grown maize (Zea mays, cultivar 'Sweet Belle') at the Plant Breeding Unit facility, E.V. Smith Research Center	Zea mays	Tallassee, AL	USA	USA	North America
124030	Field grown maize, stem-endopyte		Tallassee, Alabama, USA	United States of America	USA	North America	1990-05-02

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
24271	1	Risk group (German classification)
124030	1	Risk group (French classification)

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	NCBI tax ID	Score
124043	ASM4243234v1 assembly for Nocardioides zeicaulis CCM 8654	scaffold	GCA_042432345	1776857	57.57

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
24271	Nocardioides zeicaulis strain JM-601 16S ribosomal RNA gene, partial sequence	KU201963	1425		1776857
124043	Nocardioides zeicaulis strain CCM 8654 16S ribosomal RNA gene, partial sequence.	MT760232	1338		1776857

Genome-based predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Nocardioides zeicaulis sp. nov., an endophyte actinobacterium of maize.	Kampfer P, Glaeser SP, McInroy JA, Busse HJ	Int J Syst Evol Microbiol	10.1099/ijsem.0.000959	2016

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#24271	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 101604
#42168	; Curators of the CIP;
#43685	Peter Kämpfer, Stefanie P. Glaeser, John A. McInroy, Hans-Jürgen Busse: Nocardioides zeicaulis sp. nov., an endophyte actinobacterium of maize. IJSEM 66: 1869 - 1874 2016 ( DOI 10.1099/ijsem.0.000959 , PubMed 26867539 )
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#124030	Collection of Institut Pasteur ; Curators of the CIP; CIP 110980
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Nocardioides zeicaulis (DSM 101604, CCM 8654, CIP 110980)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Observation

Metabolite utilization

Metabolite production

Enzymes

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_042432345

16S sequences

Genome-based predictions

Literature

Literature

References

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