Amycolatopsis australiensis (DSM 44671, CIP 109398, NBRC 106146)

Name: Amycolatopsis australiensis (DSM 44671, CIP 109398, NBRC 106146)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 13248

Type strain

Strain Designation GY048

Culture col. no. DSM 44671 CIP 109398 NBRC 106146 NCIMB 14142 GY048 1 more

NCBI tax ID(s) 546364

Special Collections DSMZ JCM CIP Wink compendium

History <- M. Goodfellow; GY048 <- G. Y. A. Tan NCIMB 14142 <-- A. L. Jones <-- G. Y. A. Tan GY048. CIP <- 2006, DSMZ <- M. Goodfellow <- G.Y.A. Tan, Newcastle Univ., Newcastle upon Tyne, UK: strain GY048

Links

Amycolatopsis australiensis GY048 is an obligate aerobe, mesophilic, Gram-positive prokaryote that builds an aerial mycelium and was isolated from composite soil.

Gram-positive rod-shaped obligate aerobe mesophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 297126

NBRC 106146,DSM 44671,NCIMB 14142,JCM 15587,CIP 109398

@ref 20215

Last LPSN update 2026-02-09 11:21:05

Domain Bacteria

Phylum Actinomycetota

Class Actinomycetes

Order Pseudonocardiales

Family Pseudonocardiaceae

Genus Amycolatopsis

Species Amycolatopsis australiensis

Full scientific name Amycolatopsis australiensis Tan et al. 2006

BacDive ID	Other strains from **Amycolatopsis australiensis** (3)	Type strain
13249	A. australiensis GY056, DSM 44672
13250	A. australiensis GY269, DSM 44673
13251	A. australiensis GY276, DSM 44674

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Confidence
116647	positive	rod-shaped
125439	positive		99.8

Colony morphology

@ref	Colony color	Incubation period	Medium used
20092	Sand yellow (1002)	10-14 days	ISP 2
20092	Beige (1001)	10-14 days	ISP 3
20092	Beige (1001)	10-14 days	ISP 4
20092	Beige (1001)	10-14 days	ISP 5
20092	Beige (1001)	10-14 days	ISP 6
20092	Beige (1001)	10-14 days	ISP 7

Multicellular morphology

@ref	Complex name	Complex color	Medium name
20092	Aerial Mycelium	Pure white (9010)	ISP 2
20092	Aerial Mycelium	Pure white (9001)	ISP 3
20092	Aerial Mycelium	Pure white (9001)	ISP 4
20092	Aerial Mycelium		ISP 5
20092			ISP 6
20092	Aerial Mycelium	White	ISP 7

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
11968	GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)	Medium recipe at MediaDive	Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
20092	ISP 2		Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
20092	ISP 3		Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
20092	ISP 4		Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
20092	ISP 5		Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
20092	ISP 6		Name: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
20092	ISP 7		Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
37544	MEDIUM 57 - for Streptomyces, Nocardioides, Lentzea albidocapillata and Streptoverticillium reticulum		Distilled water make up to (1000.000 ml);Agar (15.000 g);Glucose (4.000g);Yeast extract (4.000 g);Malt extract (10.000 g);Calcium carbonate (2.000 g)
116647	CIP Medium 57	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
11968	positive	growth	28	mesophilic
20092	positive	optimum	28	mesophilic
37544	positive	growth	30	mesophilic
67770	positive	growth	28	mesophilic

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
116647	obligate aerobe
125439	obligate aerobe	99.6

Observation

67770

Observationquinones: MK-9(H₄), MK-8(H₂), MK-9(H₆)

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
20092	22599 ChEBI	arabinose	+
20092	62968 ChEBI	cellulose	+
20092	28757 ChEBI	fructose	+
20092	17234 ChEBI	glucose	+
20092	29864 ChEBI	mannitol	+
20092	17268 ChEBI	myo-inositol	+
116647	17632 ChEBI	nitrate	-	reduction
116647	16301 ChEBI	nitrite	-	reduction
20092	16634 ChEBI	raffinose	+
20092	26546 ChEBI	rhamnose	-
20092	17992 ChEBI	sucrose	+
20092	18222 ChEBI	xylose	-

Metabolite production

@ref	Chebi-ID	Metabolite	Production
116647	35581 ChEBI	indole

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68382	alpha-chymotrypsin	+	3.4.21.1	from API zym
68382	alpha-fucosidase	+	3.2.1.51	from API zym
68382	alpha-galactosidase	+	3.2.1.22	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68382	alpha-mannosidase	+	3.2.1.24	from API zym
68382	beta-galactosidase	+	3.2.1.23	from API zym
68382	beta-glucosidase	+	3.2.1.21	from API zym
116647	catalase	+	1.11.1.6
68382	cystine arylamidase	+	3.4.11.3	from API zym
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	N-acetyl-beta-glucosaminidase	+	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
116647	oxidase	-
68382	trypsin	+	3.4.21.4	from API zym
116647	urease	+	3.5.1.5
68382	valine arylamidase	+		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
20092	not determinedn.d.	+	+	+	+	+	+	+	+	+	+	+	+	+	-	+	+	+	+	+
116647	-	+	+	+	-	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Engineered	#Biodegradation	#Composting
#Environmental	#Terrestrial	#Soil

Isolation

@ref	Sample type	Country	Country ISO 3 Code	Continent
11968	composite soil	Australia	AUS	Australia and Oceania
67770	Arid Australian soil
116647	Environment, Soil, composite	Australia	AUS	Australia and Oceania

Taxonmaps

69479

Global distribution of 16S sequence AY129753 (>99% sequence identity) for Amycolatopsis australiensis from Microbeatlas

Aquatic Samples	6
Soil Samples	99
Animal Samples	12
Plant Samples	29
Total Samples	146

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
11968	1	Risk group (German classification) According to TRBA 466
116647	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
67770	IMG-taxon 2642422517 annotated assembly for Amycolatopsis australiensis DSM 44671	contig	GCA_900119165	546364.3	2642422517	546364	76.62

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
11968	Amycolatopsis australiensis strain GY048 16S ribosomal RNA gene, partial sequence	AY129753	1461		546364

GC content

@ref	GC-content (mol%)	Method
67770	71.9	genome sequence analysis

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Amycolatopsis australiensis DSM 44671
NCBI: GCA_900119165

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	yes	78.90	no
125439	motility	BacteriaNetⓘ	no	82.50	no
125439	gram_stain	BacteriaNetⓘ	positive	99.80	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	99.60	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Amycolatopsis australiensis strain DSM 44671
PATRIC: 546364.3

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	87.66	no
125438	anaerobic	anaerobicⓘ	no	97.90	yes
125438	spore-forming	spore-formingⓘ	yes	73.88	no
125438	thermophilic	thermophileⓘ	no	96.99	yes
125438	aerobic	aerobicⓘ	yes	82.49	yes
125438	flagellated	motile2+ⓘ	no	87.87	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Genetics	A Genomic Survey of the Natural Product Biosynthetic Potential of Actinomycetes Isolated from New Zealand Lichens.	Hou P, Nowak VV, Taylor CJ, Calcott MJ, Knight A, Owen JG.	mSystems	10.1128/msystems.01030-22	2023
Phylogeny	Amycolatopsis australiensis sp. nov., an actinomycete isolated from arid soils.	Tan GYA, Robinson S, Lacey E, Goodfellow M	Int J Syst Evol Microbiol	10.1099/ijs.0.64260-0	2006

References

#11968	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 44671
#20092	Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#37544	; Curators of the CIP;
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#116647	Collection of Institut Pasteur ; Curators of the CIP; CIP 109398
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Amycolatopsis australiensis (DSM 44671, CIP 109398, NBRC 106146)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Multicellular morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Observation

Metabolite utilization

Metabolite production

Enzymes

API zym

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_900119165

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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