Pseudoclavibacter endophyticus EGI 60007 | Type strain | DSM 29943, CGMCC 1.15081, KCTC 39112

Strain identifier

BacDive ID: 132433

Type strain:

Species: Pseudoclavibacter endophyticus

Strain Designation: EGI 60007

Culture col. no.: DSM 29943, CGMCC 1.15081, KCTC 39112

Section

Name and taxonomic classification
Morphology and physiology
Culture and growth conditions
Isolation, sampling and environmental information
Molecular biology
Strain availability

External links

Information on the name and the taxonomic classification.Name and taxonomic classification

	Last LPSN update	31-03-2020 (DD-MM-YYYY)
	Domain	Bacteria
	Phylum	Actinobacteria
	Class	Actinobacteria
	Order	Micrococcales
	Family	Microbacteriaceae
	Genus	Pseudoclavibacter
	Species	Pseudoclavibacter endophyticus
	Full Scientific Name (PNU)	Pseudoclavibacter endophyticus Li et al. 2016

Information on morphological and physiological propertiesMorphology and physiology

[Ref.: #43744]	Gram stain	positive
[Ref.: #43744]	Cell length	1.1-2.3 µm
[Ref.: #43744]	Cell width	0.4-0.6 µm
[Ref.: #43744]	Cell shape	rod-shaped
[Ref.: #43744]	Motility	no

[Ref.: #43744]	Colony color	White, opaque
[Ref.: #43744]	Colony shape	circular
[Ref.: #43744]	Incubation period	7 days
[Ref.: #43744]	Cultivation medium used	R2A agar

	Enzyme	Enzyme activity	EC number
[Ref.: #43744]	acid phosphatase	-	3.1.3.2
[Ref.: #43744]	alkaline phosphatase	-	3.1.3.1
[Ref.: #43744]	alpha-galactosidase	-	3.2.1.22
[Ref.: #43744]	alpha-glucosidase	-	3.2.1.20
[Ref.: #43744]	alpha-mannosidase	-	3.2.1.24
[Ref.: #43744]	beta-D-fucosidase	-	3.2.1.38
[Ref.: #43744]	beta-galactosidase	-	3.2.1.23
[Ref.: #43744]	beta-glucosidase	+	3.2.1.21
[Ref.: #43744]	beta-glucuronidase	-	3.2.1.31
[Ref.: #43744]	catalase	+	1.11.1.6
[Ref.: #43744]	chymotrypsin	+	3.4.4.5
[Ref.: #43744]	cystine arylamidase	+	3.4.11.3
[Ref.: #43744]	cytochrome oxidase	-	1.9.3.1
[Ref.: #43744]	esterase (C 4)	+
[Ref.: #43744]	esterase Lipase (C 8)	+
[Ref.: #43744]	gelatinase	-
[Ref.: #43744]	leucine arylamidase	+	3.4.11.1
[Ref.: #43744]	lipase (C 14)	-
[Ref.: #43744]	N-acetyl-beta-glucosaminidase	-	3.2.1.52
[Ref.: #43744]	naphthol-AS-BI-phosphohydrolase	+
[Ref.: #43744]	trypsin	-	3.4.21.4
[Ref.: #43744]	urease	-	3.5.1.5
[Ref.: #43744]	valine arylamidase	+
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Type of FA analysis

whole cell analysis

Incubation medium

TSA

Agar/liquid

agar

Incubation temperature (°C)

Incubation time (d)

Incubation pH

Incubation oxygen condition

aerobic

Treatment/condition

Software version

Sherlock 6.1

Library/peak naming table

TSBA6

System

MIS MIDI

Instrument

Agilent 7890A

Method/protocol

Cutoff value

1.00

Reference

[Ref.: #43744]

Fatty acid	Percentage
C10:0 3OH	1.1
C12:0	10.9
C12:0 3OH	2.8
C14:0	0.8
C16:0	11.6
C16:1ω5c	0.5
C16:1ω7c / C16:1ω6c	8.2
C17:0	0.8
C17:0cyclo	13
C17:1ω8c	0.9
C18:0	0.6
C18:1ω7c / C18:1ω6c	18.3
C18:1ω9c	0.4
C19:0ω8c-cyclo	9.3
C11:0 iso 3OH	0.3

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #43744]		NaCl		growth	0-11	%(w/v)
[Ref.: #43744]		NaCl		optimum	4	%(w/v)

	Metabolite	Antibiotic sensitivity
[Ref.: #43744]	1 % sodium lactate	yes
[Ref.: #43744]	aztreonam ChEBI	yes
[Ref.: #43744]	lincomycin ChEBI	yes
[Ref.: #43744]	nalidixic acid ChEBI	yes
[Ref.: #43744]	potassium tellurite ChEBI	yes
[Ref.: #43744]	sodium bromate ChEBI	yes
[Ref.: #43744]	sodium butyrate ChEBI	yes
[Ref.: #43744]	tetrazolium violet ChEBI	yes
[Ref.: #43744]	troleandomycin ChEBI	yes

	Metabolite production	Metabolite	Production
[Ref.: #43744]		indole ChEBI	yes

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #43744]	(S)-lactate ChEBI	-	respiration
[Ref.: #43744]	2-hydroxybutyrate ChEBI	-	respiration
[Ref.: #43744]	2-oxobutanoate ChEBI	-	respiration
[Ref.: #43744]	2-oxoglutarate ChEBI	-	respiration
[Ref.: #43744]	4-hydroxyphenylacetic acid ChEBI	+	respiration
[Ref.: #43744]	acetate ChEBI	+	respiration
[Ref.: #43744]	acetoacetate ChEBI	+	respiration
[Ref.: #43744]	arabinose ChEBI	-	builds acid from
[Ref.: #43744]	beta-hydroxybutyrate ChEBI	+	respiration
[Ref.: #43744]	citrate ChEBI	-	respiration
[Ref.: #43744]	D-fructose ChEBI	-	builds acid from
[Ref.: #43744]	D-fructose ChEBI	+	respiration
[Ref.: #43744]	D-fructose 6-phosphate ChEBI	-	respiration
[Ref.: #43744]	D-gluconate ChEBI	-	respiration
[Ref.: #43744]	D-glucose 6-phosphate ChEBI	-	respiration
[Ref.: #43744]	D-malate ChEBI	-	respiration
[Ref.: #43744]	D-mannitol ChEBI	+	respiration
[Ref.: #43744]	D-mannose ChEBI	-	builds acid from
[Ref.: #43744]	D-xylose ChEBI	-	builds acid from
[Ref.: #43744]	esculin ChEBI	+	fermentation
[Ref.: #43744]	formate ChEBI	+	respiration
[Ref.: #43744]	gelatin ChEBI	-	hydrolysis
[Ref.: #43744]	glucose ChEBI	-	builds acid from
[Ref.: #43744]	glycerol ChEBI	-	builds acid from
[Ref.: #43744]	glycerol ChEBI	-	respiration
[Ref.: #43744]	L-aspartate ChEBI	+	respiration
[Ref.: #43744]	L-glutamate ChEBI	+	respiration
[Ref.: #43744]	L-malate ChEBI	-	respiration
[Ref.: #43744]	mannitol ChEBI	-	builds acid from
[Ref.: #43744]	melibiose ChEBI	-	builds acid from
[Ref.: #43744]	methyl pyruvate ChEBI	-	respiration
[Ref.: #43744]	myo-inositol ChEBI	-	builds acid from
[Ref.: #43744]	myo-inositol ChEBI	-	respiration
[Ref.: #43744]	N-acetylglucosamine ChEBI	-	builds acid from
[Ref.: #43744]	N-acetylglucosamine ChEBI	-	respiration
[Ref.: #43744]	pectin ChEBI	-	respiration
[Ref.: #43744]	propionate ChEBI	-	respiration
[Ref.: #43744]	rhamnose ChEBI	-	builds acid from
[Ref.: #43744]	sorbitol ChEBI	-	builds acid from
[Ref.: #43744]	sucrose ChEBI	-	builds acid from
	Only first 10 entries are displayed. Click here to see all.

[Ref.: #43744]

Oxygen tolerance

aerobe

Information on culture and growth conditionsCulture and growth conditions

[Ref.: #43744]

Culture medium

GYEA

[Ref.: #43744]

Culture medium growth

[Ref.: #43744]

Culture medium

LB (Luria-Bertani) MEDIUM

[Ref.: #43744]

Culture medium growth

[Ref.: #43744]

Culture medium

Reasoner's 2A agar (R2A)

[Ref.: #43744]

Culture medium growth

[Ref.: #43744]

Culture medium

Trypticase Soy Agar (TSA)

[Ref.: #43744]

Culture medium growth

[Ref.: #24222]

Culture medium

TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)

[Ref.: #24222]

Culture medium growth

[Ref.: #24222]

Culture medium link

medium recipe provided by DSMZ

		Temperature
[Ref.: #24222]	growth	28 ̊C
[Ref.: #43744]	growth	10-60 ̊C
[Ref.: #43744]	optimum	25-35 ̊C

[Ref.: #24222]	Temperature range	mesophilic
[Ref.: #43744]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #43744]			optimum	8
[Ref.: #43744]			growth	7-9

Information on isolation source, the sampling and environmental conditionsIsolation, sampling and environmental information

[Ref.: #24222]

Sample type/isolated from

surface-sterilized healthy roots of Glycyrrhiza uralensis F.

[Ref.: #24222]

Host species

Glycyrrhiza uralensis

[Ref.: #24222]

Geographic location (country and/or sea, region)

Xinjiang Province, Yili County (43.39° N 83.84° E)

[Ref.: #24222]

Country

China

[Ref.: #24222]

Country ISO 3 Code

CHN

[Ref.: #24222]

Continent

Asia

[Ref.: #24222]

Geographic location

43.3900°/83.8400°

[Ref.: #43744]

Sample type/isolated from

Healthy root of Glycyrrhiza uralensis F. collected from Yili County

[Ref.: #43744]

Geographic location (country and/or sea, region)

Yili County, Xinjiang Province

[Ref.: #43744]

Country

China

[Ref.: #43744]

Country ISO 3 Code

CHN

[Ref.: #43744]

Continent

Asia

Isolation sources categories

#Host Body-Site	#Plant	#Sterilized plant part
#Host	#Plants	#Herbaceous plants (Grass,Crops)
#Host Body-Site	#Plant	#Root (Rhizome)

What are isolation sources categories?

* marker position based on {}

Information on genomic background e.g. entries in nucleic sequence databassMolecular biology

[Ref.: #43744]	GC-content	63.3 mol%	high performance liquid chromatography (HPLC)
[Ref.: #24222]	GC-content	63.3 mol%	high performance liquid chromatography (HPLC)

Sequence information:

	Sequence database	Sequence accession description	Sequence accession number	Sequence type	Sequence length (bp)	Associated NCBI tax ID
[Ref.: #24222]	16S rRNA gene, Marker Gene (GenBank Direct submission)	Pseudoclavibacter endophyticus strain EGI 60007 16S ribosomal RNA gene, partial sequence	KP721484 ENA	16S	1490	1778590 tax ID

Availability in culture collectionsStrain availability

[Ref.: #24222]

Culture collection no.

DSM 29943, CGMCC 1.15081, KCTC 39112

[Ref.: #24222]

Strain history

<- W.-J. Li, YIM; EGI60007 <- L. Li

References

#20215

Leibniz Institute DSMZ D.Gleim, M.Kracht, N.Weiss et. al.: Prokaryotic Nomenclature Up-to-date - compilation of all names of Bacteria and Archaea, validly published according to the Bacteriological Code since 1. Jan. 1980, and validly published nomenclatural changes since .

#24222

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 29943

#43744

Yan-Qiong Li, Li Li, Yu-Song Fu, Zhao-Qiong Cui, Yan-Qing Duan, Nimaichand Salam, Jian-Wei Guo, Wei Chen, Wen-Jun Li: Pseudoclavibacter endophyticus sp. nov., isolated from roots of Glycyrrhiza uralensis. IJSEM 66: 1287 - 1292 2016 ( DOI 10.1099/ijsem.0.000876 )

* These References are textmined

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