Amycolatopsis balhimycina FH 1894 is an obligate aerobe, Gram-positive, rod-shaped bacterium that builds an aerial mycelium and produces antibiotic compounds.
antibiotic compound production Gram-positive rod-shaped obligate aerobe genome sequence 16S sequence Bacteria| @ref 20215 |
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| Domain Bacteria |
| Phylum Actinomycetota |
| Class Actinomycetes |
| Order Pseudonocardiales |
| Family Pseudonocardiaceae |
| Genus Amycolatopsis |
| Species Amycolatopsis balhimycina |
| Full scientific name Amycolatopsis balhimycina Wink et al. 2003 |
| BacDive ID | Other strains from Amycolatopsis balhimycina (1) | Type strain |
|---|---|---|
| 104705 | A. balhimycina SF006185, |
| @ref | Colony color | Incubation period | Medium used | Hemolysis ability | |
|---|---|---|---|---|---|
| 20077 | Daffodil yellow (1007) | 10-14 days | ISP 2 | ||
| 20077 | Daffodil yellow (1007) | 10-14 days | ISP 3 | ||
| 20077 | Daffodil yellow (1007) | 10-14 days | ISP 4 | ||
| 20077 | Daffodil yellow (1007) | 10-14 days | ISP 5 | ||
| 20077 | Daffodil yellow (1007) | 10-14 days | ISP 6 | ||
| 20077 | Daffodil yellow (1007) | 10-14 days | ISP 7 | ||
| 116716 | 0 |
| @ref | Name | Growth | Medium link | Composition | |
|---|---|---|---|---|---|
| 11906 | GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) | Medium recipe at MediaDive | Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water | ||
| 20077 | ISP 2 | Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes | |||
| 20077 | ISP 3 | Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms: | |||
| 20077 | ISP 4 | Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml | |||
| 20077 | ISP 5 | Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes | |||
| 20077 | ISP 6 | Name: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes | |||
| 20077 | ISP 7 | Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes | |||
| 40012 | MEDIUM 57 - for Streptomyces, Nocardioides, Lentzea albidocapillata and Streptoverticillium reticulum | Distilled water make up to (1000.000 ml);Agar (15.000 g);Glucose (4.000g);Yeast extract (4.000 g);Malt extract (10.000 g);Calcium carbonate (2.000 g) | |||
| 116716 | CIP Medium 57 | Medium recipe at CIP |
| 67770 | Observationquinones: MK-9(H4) |
| @ref | Chebi-ID | Metabolite | Utilization activity | Kind of utilization tested | |
|---|---|---|---|---|---|
| 20077 | 22599 ChEBI | arabinose | + | ||
| 68368 | 29016 ChEBI | arginine | - | hydrolysis | from API 20E |
| 20077 | 62968 ChEBI | cellulose | +/- | ||
| 116716 | 16947 ChEBI | citrate | - | carbon source | |
| 68368 | 16947 ChEBI | citrate | + | assimilation | from API 20E |
| 116716 | 4853 ChEBI | esculin | + | hydrolysis | |
| 20077 | 28757 ChEBI | fructose | + | ||
| 68368 | 5291 ChEBI | gelatin | + | hydrolysis | from API 20E |
| 20077 | 17234 ChEBI | glucose | + | ||
| 116716 | 606565 ChEBI | hippurate | + | hydrolysis | |
| 68368 | 25094 ChEBI | lysine | - | degradation | from API 20E |
| 20077 | 29864 ChEBI | mannitol | + | ||
| 20077 | 17268 ChEBI | myo-inositol | + | ||
| 116716 | 17632 ChEBI | nitrate | - | reduction | |
| 116716 | 17632 ChEBI | nitrate | - | respiration | |
| 116716 | 16301 ChEBI | nitrite | - | reduction | |
| 68368 | 18257 ChEBI | ornithine | - | degradation | from API 20E |
| 20077 | 16634 ChEBI | raffinose | +/- | ||
| 20077 | 26546 ChEBI | rhamnose | + | ||
| 20077 | 17992 ChEBI | sucrose | + | ||
| 68368 | 27897 ChEBI | tryptophan | - | energy source | from API 20E |
| 68368 | 16199 ChEBI | urea | + | hydrolysis | from API 20E |
| 20077 | 18222 ChEBI | xylose | - |
| @ref | Metabolite | Is sensitive | Is resistant | |
|---|---|---|---|---|
| 116716 | 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate) |
| @ref | Value | Activity | Ec | |
|---|---|---|---|---|
| 68382 | acid phosphatase | + | 3.1.3.2 | from API zym |
| 116716 | alcohol dehydrogenase | - | 1.1.1.1 | |
| 68382 | alkaline phosphatase | + | 3.1.3.1 | from API zym |
| 68382 | alpha-glucosidase | + | 3.2.1.20 | from API zym |
| 68382 | alpha-mannosidase | - | 3.2.1.24 | from API zym |
| 116716 | amylase | + | ||
| 68368 | arginine dihydrolase | - | 3.5.3.6 | from API 20E |
| 68382 | beta-galactosidase | + | 3.2.1.23 | from API zym |
| 116716 | beta-galactosidase | + | 3.2.1.23 | |
| 68368 | beta-galactosidase | - | 3.2.1.23 | from API 20E |
| 68382 | beta-glucuronidase | - | 3.2.1.31 | from API zym |
| 116716 | caseinase | - | 3.4.21.50 | |
| 116716 | catalase | + | 1.11.1.6 | |
| 116716 | DNase | - | ||
| 68382 | esterase (C 4) | + | from API zym | |
| 68382 | esterase lipase (C 8) | + | from API zym | |
| 116716 | gamma-glutamyltransferase | + | 2.3.2.2 | |
| 116716 | gelatinase | - | ||
| 68368 | gelatinase | + | from API 20E | |
| 116716 | lecithinase | - | ||
| 68382 | leucine arylamidase | + | 3.4.11.1 | from API zym |
| 116716 | lipase | - | ||
| 116716 | lysine decarboxylase | - | 4.1.1.18 | |
| 68368 | lysine decarboxylase | - | 4.1.1.18 | from API 20E |
| 68382 | N-acetyl-beta-glucosaminidase | + | 3.2.1.52 | from API zym |
| 116716 | ornithine decarboxylase | - | 4.1.1.17 | |
| 68368 | ornithine decarboxylase | - | 4.1.1.17 | from API 20E |
| 116716 | oxidase | - | ||
| 116716 | tryptophan deaminase | - | ||
| 68368 | tryptophan deaminase | - | 4.1.99.1 | from API 20E |
| 116716 | tween esterase | + | ||
| 116716 | urease | + | 3.5.1.5 | |
| 68368 | urease | + | 3.5.1.5 | from API 20E |
Global distribution of 16S sequence AJ508239 (>99% sequence identity) for Amycolatopsis from Microbeatlas ![]()
| @ref | Description | Assembly level | INSDC accession | BV-BRC accession | IMG accession | NCBI tax ID | Score | |
|---|---|---|---|---|---|---|---|---|
| 67770 | ASM38429v1 assembly for Amycolatopsis balhimycina FH 1894 DSM 44591 | scaffold | 1089545 | 77.06 |
| @ref | Description | Accession | Length | Database | NCBI tax ID | |
|---|---|---|---|---|---|---|
| 11906 | Amycolatopsis balhimycetica 16S rRNA gene, type strain DSM 44591T | AJ508239 | 1449 | 208443 |
| @ref | GC-content (mol%) | Method | |
|---|---|---|---|
| 67770 | 70.8 | genome sequence analysis |
| @ref | Trait | Model | Prediction | Confidence in % | In training data |
|---|---|---|---|---|---|
| 125439 | oxygen_tolerance | BacteriaNetⓘ | obligate aerobe | 99.17 | no |
| 125439 | motility | BacteriaNetⓘ | no | 88.04 | no |
| 125439 | gram_stain | BacteriaNetⓘ | positive | 98.13 | no |
| 125439 | spore_formation | BacteriaNetⓘ | yes | 60.09 | no |
| @ref | Trait | Model | Prediction | Confidence in % | In training data |
|---|---|---|---|---|---|
| 125438 | gram-positive | gram-positiveⓘ | yes | 87.21 | no |
| 125438 | anaerobic | anaerobicⓘ | no | 96.88 | yes |
| 125438 | spore-forming | spore-formingⓘ | yes | 68.83 | no |
| 125438 | aerobic | aerobicⓘ | yes | 83.12 | no |
| 125438 | thermophilic | thermophileⓘ | no | 96.50 | no |
| 125438 | flagellated | motile2+ⓘ | no | 86.50 | no |
| Topic | Title | Authors | Journal | DOI | Year | |
|---|---|---|---|---|---|---|
| The Impact of Heterologous Regulatory Genes from Lipodepsipeptide Biosynthetic Gene Clusters on the Production of Teicoplanin and A40926. | Zhukrovska K, Binda E, Fedorenko V, Marinelli F, Yushchuk O. | Antibiotics (Basel) | 10.3390/antibiotics13020115 | 2024 | ||
| Unveiling the substrate specificity of the ABC transporter Tba and its role in glycopeptide biosynthesis. | Gericke N, Beqaj D, Kronenberger T, Kulik A, Gavriilidou A, Franz-Wachtel M, Schoppmeier U, Harbig T, Rapp J, Grin I, Ziemert N, Link H, Nieselt K, Macek B, Wohlleben W, Stegmann E, Wagner S. | iScience | 10.1016/j.isci.2025.112135 | 2025 | ||
| Genetics | Genome neighborhood network reveals insights into enediyne biosynthesis and facilitates prediction and prioritization for discovery. | Rudolf JD, Yan X, Shen B. | J Ind Microbiol Biotechnol | 10.1007/s10295-015-1671-0 | 2016 | |
| A proof-reading mechanism for non-proteinogenic amino acid incorporation into glycopeptide antibiotics. | Kaniusaite M, Tailhades J, Marschall EA, Goode RJA, Schittenhelm RB, Cryle MJ. | Chem Sci | 10.1039/c9sc03678d | 2019 | ||
| Genetics | Distribution in microbial genomes of genes similar to lodA and goxA which encode a novel family of quinoproteins with amino acid oxidase activity. | Campillo-Brocal JC, Chacon-Verdu MD, Lucas-Elio P, Sanchez-Amat A. | BMC Genomics | 10.1186/s12864-015-1455-y | 2015 | |
| Phylogeny | Three new antibiotic producing species of the genus Amycolatopsis, Amycolatopsis balhimycina sp. nov., A. tolypomycina sp. nov., A. vancoresmycina sp. nov., and description of Amycolatopsis keratiniphila subsp. keratiniphila subsp. nov. and A. keratiniphila subsp. nogabecina subsp. nov. | Wink JM, Kroppenstedt RM, Ganguli BN, Nadkarni SR, Schumann P, Seibert G, Stackebrandt E | Syst Appl Microbiol | 10.1078/072320203322337290 | 2003 |
| #11906 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 44591 |
| #20077 | Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig . |
| #20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 ) |
| #20216 | Curators of the JMRC: Jena Microbial Resource Collection (JMRC): |
| #40012 | ; Curators of the CIP; |
| #67770 | Japan Collection of Microorganism (JCM) ; Curators of the JCM; |
| #68368 | Automatically annotated from API 20E . |
| #68382 | Automatically annotated from API zym . |
| #69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta . |
| #116716 | Collection of Institut Pasteur ; Curators of the CIP; CIP 108516 |
| #125438 | Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 ) |
| #125439 | Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 . |
| #126262 | A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 ) |
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